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The map label for this gene is pmbA [H]

Identifier: 89052894

GI number: 89052894

Start: 405127

End: 406476

Strand: Reverse

Name: pmbA [H]

Synonym: Jann_0403

Alternate gene names: 89052894

Gene position: 406476-405127 (Counterclockwise)

Preceding gene: 89052895

Following gene: 89052893

Centisome position: 9.41

GC content: 67.56

Gene sequence:

>1350_bases
ATGACAGACACGCAACTCGCGCCCCTTGCCCACGCCCTTCTGGAGGCTGCCACCCGCGCCGGTGCAGATGCCGCCGATGC
CCTTGTGGTGGATGGCACTTCCGTCAGCATCGGCGTTCTCAACGGCGCGCTGGAGCAGGCCGAGCGATCCGAGGGGATCG
ACATGGGCCTGCGGGTTCTGGTCGGCCAACGGCAGGCCTGCGTGTCTGCGTCTGATACATCTGCGGCCACGGTCACGGAC
ATGGCCGAGCGCGCCGTCGCCATGGCGCGCCTTGCCCCGGAAGATCCCCATGCGGGTCTGGCAGATGTGGACCAGCTCAG
CACCGTTCGTGACGGGGCCGCACTGGACATGAATGATCCGGGCACGGACCCGGACCCCGCCGCGTTGGAGGCCATGGCGC
TGGAGGCGGAGGCGGCCGCGCTGGCTGTTGACGGCATTTCGCAGGTCTCGGATGCAGGTGCGGGCTTCTCGCGGCGTCAG
ATCTTTCTGGCGGCAACCAACGGTTTTGCGGGCGGCTATGCGCGCACGGACCACGGCCTTCACTGCATTGCAATCACGGG
CGAAGGCGCGTCGATGGAGCGGGATTACTTCGGCGACGGGCGCAACCACGCGGGCGACCTGATGTCGCCAGCGGATGTCG
GGCGCATCGCGGCAGAGCGAACCGTGGCGCGCGCAGGCGCAAGGAAACCGCCGACCGGAGCGTTTCCGGTCATGTATGAC
GAACGCGTGGCGGGCGGCCTGATCGGCCACCTTCTGGCCGCCACCAACGGCACGGCAATTGCCCGCGGCGCGTCGTGGGC
GCGGGATCTTCTGGGGGACCAGATTTTGCCCAGGGGCATCTCCCTGATCGAGGAGCCGCACCGCCCGCGCACCTCTGCCA
GCCGTCCGTTCGACGCAGAGGGCCTGCCCACTGCGGATCGCCTGATTGTATCGGACGGCGTGCTCACGGGCTGGACACTG
GACCTTTCGACCGCGCGCAAACTGGGGCTGACCTCCACCGCGTCGGCCACGCGCAGCACCGGCGCCCCACCATCTCCGTC
CGCTGGTAATGCATGCCTGACAGAGGGGGAGCGCACCCGGGAGGACCTGTTGAAGGAGATGGGAACCGGTTTGCTGATCA
CCTCGCTCATCGGCTCTTCCATCAACGCCACCACGGGCGATTACTCTCGCGGTGCATCGGGTTTCTGGGTGGAGAACGGA
GAGATCACCCACGCCGTCAATGAATGCACGGTCGCGGGTAATCTGCGCGACATGCTGCGCGGGATCGTGCCTGCCAATGA
CGCCCGCGCGCATCTCAGCCGTGTGGTGCCGTCGCTTCTCGTCGAGGGGCTGACGATTGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
GACCGATGGTTGCGACGGCCCGGGGCACCTGCTTCGGGCCGTTTCGCTTGCAAGCCCCCGGTCGCGGCTCTACCTCCAAG
GCAACAGACTGAGGTTTCCC

Downstream 100 bases:

>100_bases
CGACGATTTACAACTCCTGATCGCCGCTGCCCTACGCGCCGGTGAGATCGCGAAATTGCACTTTGGCAACGATCCGAAAG
TCTATGACAAGGGCGACGAA

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTDTQLAPLAHALLEAATRAGADAADALVVDGTSVSIGVLNGALEQAERSEGIDMGLRVLVGQRQACVSASDTSAATVTD
MAERAVAMARLAPEDPHAGLADVDQLSTVRDGAALDMNDPGTDPDPAALEAMALEAEAAALAVDGISQVSDAGAGFSRRQ
IFLAATNGFAGGYARTDHGLHCIAITGEGASMERDYFGDGRNHAGDLMSPADVGRIAAERTVARAGARKPPTGAFPVMYD
ERVAGGLIGHLLAATNGTAIARGASWARDLLGDQILPRGISLIEEPHRPRTSASRPFDAEGLPTADRLIVSDGVLTGWTL
DLSTARKLGLTSTASATRSTGAPPSPSAGNACLTEGERTREDLLKEMGTGLLITSLIGSSINATTGDYSRGASGFWVENG
EITHAVNECTVAGNLRDMLRGIVPANDARAHLSRVVPSLLVEGLTIAGA

Sequences:

>Translated_449_residues
MTDTQLAPLAHALLEAATRAGADAADALVVDGTSVSIGVLNGALEQAERSEGIDMGLRVLVGQRQACVSASDTSAATVTD
MAERAVAMARLAPEDPHAGLADVDQLSTVRDGAALDMNDPGTDPDPAALEAMALEAEAAALAVDGISQVSDAGAGFSRRQ
IFLAATNGFAGGYARTDHGLHCIAITGEGASMERDYFGDGRNHAGDLMSPADVGRIAAERTVARAGARKPPTGAFPVMYD
ERVAGGLIGHLLAATNGTAIARGASWARDLLGDQILPRGISLIEEPHRPRTSASRPFDAEGLPTADRLIVSDGVLTGWTL
DLSTARKLGLTSTASATRSTGAPPSPSAGNACLTEGERTREDLLKEMGTGLLITSLIGSSINATTGDYSRGASGFWVENG
EITHAVNECTVAGNLRDMLRGIVPANDARAHLSRVVPSLLVEGLTIAGA
>Mature_448_residues
TDTQLAPLAHALLEAATRAGADAADALVVDGTSVSIGVLNGALEQAERSEGIDMGLRVLVGQRQACVSASDTSAATVTDM
AERAVAMARLAPEDPHAGLADVDQLSTVRDGAALDMNDPGTDPDPAALEAMALEAEAAALAVDGISQVSDAGAGFSRRQI
FLAATNGFAGGYARTDHGLHCIAITGEGASMERDYFGDGRNHAGDLMSPADVGRIAAERTVARAGARKPPTGAFPVMYDE
RVAGGLIGHLLAATNGTAIARGASWARDLLGDQILPRGISLIEEPHRPRTSASRPFDAEGLPTADRLIVSDGVLTGWTLD
LSTARKLGLTSTASATRSTGAPPSPSAGNACLTEGERTREDLLKEMGTGLLITSLIGSSINATTGDYSRGASGFWVENGE
ITHAVNECTVAGNLRDMLRGIVPANDARAHLSRVVPSLLVEGLTIAGA

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=431, Percent_Identity=38.5150812064965, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46061; Mature: 45930

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQLAPLAHALLEAATRAGADAADALVVDGTSVSIGVLNGALEQAERSEGIDMGLRVL
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHCCHHHHHHHH
VGQRQACVSASDTSAATVTDMAERAVAMARLAPEDPHAGLADVDQLSTVRDGAALDMNDP
HCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCCC
GTDPDPAALEAMALEAEAAALAVDGISQVSDAGAGFSRRQIFLAATNGFAGGYARTDHGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCE
HCIAITGEGASMERDYFGDGRNHAGDLMSPADVGRIAAERTVARAGARKPPTGAFPVMYD
EEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEC
ERVAGGLIGHLLAATNGTAIARGASWARDLLGDQILPRGISLIEEPHRPRTSASRPFDAE
CHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
GLPTADRLIVSDGVLTGWTLDLSTARKLGLTSTASATRSTGAPPSPSAGNACLTEGERTR
CCCCHHHEEEECCCEEEEEEEHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHCCCHHHH
EDLLKEMGTGLLITSLIGSSINATTGDYSRGASGFWVENGEITHAVNECTVAGNLRDMLR
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEHHHHHEECCCHHHHHH
GIVPANDARAHLSRVVPSLLVEGLTIAGA
HCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTQLAPLAHALLEAATRAGADAADALVVDGTSVSIGVLNGALEQAERSEGIDMGLRVL
CCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHCCHHHHHHHH
VGQRQACVSASDTSAATVTDMAERAVAMARLAPEDPHAGLADVDQLSTVRDGAALDMNDP
HCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCCC
GTDPDPAALEAMALEAEAAALAVDGISQVSDAGAGFSRRQIFLAATNGFAGGYARTDHGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCE
HCIAITGEGASMERDYFGDGRNHAGDLMSPADVGRIAAERTVARAGARKPPTGAFPVMYD
EEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEC
ERVAGGLIGHLLAATNGTAIARGASWARDLLGDQILPRGISLIEEPHRPRTSASRPFDAE
CHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
GLPTADRLIVSDGVLTGWTLDLSTARKLGLTSTASATRSTGAPPSPSAGNACLTEGERTR
CCCCHHHEEEECCCEEEEEEEHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHCCCHHHH
EDLLKEMGTGLLITSLIGSSINATTGDYSRGASGFWVENGEITHAVNECTVAGNLRDMLR
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEHHHHHEECCCHHHHHH
GIVPANDARAHLSRVVPSLLVEGLTIAGA
HCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]