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The map label for this gene is ycjG [H]

Identifier: 89052856

GI number: 89052856

Start: 368087

End: 369040

Strand: Reverse

Name: ycjG [H]

Synonym: Jann_0365

Alternate gene names: 89052856

Gene position: 369040-368087 (Counterclockwise)

Preceding gene: 89052857

Following gene: 89052855

Centisome position: 8.55

GC content: 64.47

Gene sequence:

>954_bases
ATGTTCAAGATCACGCGCGACAAATTCAAGCTGGATGAAGTGTTCACGATTTCTCGCGGGTCAAAGACCCATGCCGAAGT
GCTGACCTTTGAAATCGACCAAGGCGCCAGTGGGCGGGGGCGGGGCGAATGCGTGCCCTACGCCCGTTACGGAGAGACCC
TCGATAGCGTTGAGGCACAGATCCAGGACTACATCGACAACGGCGACAGCGCCCGTTTTCCACCCGGGGCTGCGCGGAAC
GCCATCGAATGCGCCATTCTGGATGCCGAAGCCAAGTGCAACGCCACGACTGTTTGGGAAATGCTCCGCCAGCCCGTGCC
TCAGCCCGTCACCTCGGCCTTCACCCTATCGCTGGACACGCCCGAGAAGATGCGCGCCTCTGCGGCCAAACATGCCGCGC
GCCCGCTCCTGAAAATCAAACTTGGCACAGCCGATGATATGGCCCGCCTCGAAGCGGTCCGCGCAGGCGCGCCCAAGGCC
GCGATCATCGTCGATGCCAATGAAGGCTGGACGGCCGAAATCTACACGGACCTCGCGCCCCACCTCCTCCGCCTGGGCGT
GCAGATGGTGGAACAGCCCCTGCCCGCGGGTCAGGACGATATGCTGTCCGAGATCGCCCGCCCCCTTCCCGTCTGCGCGG
ACGAATCCTGTCACGATCGCGCGTCGCTGCCCGACCTCAAGGGCAAATACGACATGGTCAATATCAAGCTCGACAAGACC
GGCGGCCTGTCCGAAGCCCTCGCGCTTCGCGATGCCGCCCGTGCCGAAGGGTACACGGTGATGGTGGGCTGCATGGTCGG
CTCATCCCTCGCCATGGCCCCTGCCATGCTCGTGGCCCAGGGGGCCGAGATCGTGGACCTCGATGGGCCCCTCCTCCTCG
CCGAAGACCGCACCCCACCGCTCAAATTCGACGGCTCCACAATCCATCCCCCGGAGGCCGCCCTCTGGGGCTGA

Upstream 100 bases:

>100_bases
TGCAAACCCTCGCCGATATCGAAAAGCGGATGGGCCTGCCCACCGCCGATCCGTTCCGCCAGGGCGCGGGCCGCCTCTGC
GATGCGCTGGATGCGATCTG

Downstream 100 bases:

>100_bases
CCGCTCTTTCATCTGGGCAATACAACTCCGGGGGCCAGGGGGCAGAGCCCCCTGACGTTCATTGTATTTTGGCAGGGCCC
ACTGACGTTCATGGTCTTGG

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MFKITRDKFKLDEVFTISRGSKTHAEVLTFEIDQGASGRGRGECVPYARYGETLDSVEAQIQDYIDNGDSARFPPGAARN
AIECAILDAEAKCNATTVWEMLRQPVPQPVTSAFTLSLDTPEKMRASAAKHAARPLLKIKLGTADDMARLEAVRAGAPKA
AIIVDANEGWTAEIYTDLAPHLLRLGVQMVEQPLPAGQDDMLSEIARPLPVCADESCHDRASLPDLKGKYDMVNIKLDKT
GGLSEALALRDAARAEGYTVMVGCMVGSSLAMAPAMLVAQGAEIVDLDGPLLLAEDRTPPLKFDGSTIHPPEAALWG

Sequences:

>Translated_317_residues
MFKITRDKFKLDEVFTISRGSKTHAEVLTFEIDQGASGRGRGECVPYARYGETLDSVEAQIQDYIDNGDSARFPPGAARN
AIECAILDAEAKCNATTVWEMLRQPVPQPVTSAFTLSLDTPEKMRASAAKHAARPLLKIKLGTADDMARLEAVRAGAPKA
AIIVDANEGWTAEIYTDLAPHLLRLGVQMVEQPLPAGQDDMLSEIARPLPVCADESCHDRASLPDLKGKYDMVNIKLDKT
GGLSEALALRDAARAEGYTVMVGCMVGSSLAMAPAMLVAQGAEIVDLDGPLLLAEDRTPPLKFDGSTIHPPEAALWG
>Mature_317_residues
MFKITRDKFKLDEVFTISRGSKTHAEVLTFEIDQGASGRGRGECVPYARYGETLDSVEAQIQDYIDNGDSARFPPGAARN
AIECAILDAEAKCNATTVWEMLRQPVPQPVTSAFTLSLDTPEKMRASAAKHAARPLLKIKLGTADDMARLEAVRAGAPKA
AIIVDANEGWTAEIYTDLAPHLLRLGVQMVEQPLPAGQDDMLSEIARPLPVCADESCHDRASLPDLKGKYDMVNIKLDKT
GGLSEALALRDAARAEGYTVMVGCMVGSSLAMAPAMLVAQGAEIVDLDGPLLLAEDRTPPLKFDGSTIHPPEAALWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=317, Percent_Identity=42.2712933753943, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34058; Mature: 34058

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKITRDKFKLDEVFTISRGSKTHAEVLTFEIDQGASGRGRGECVPYARYGETLDSVEAQ
CCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCHHHHHHHH
IQDYIDNGDSARFPPGAARNAIECAILDAEAKCNATTVWEMLRQPVPQPVTSAFTLSLDT
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCC
PEKMRASAAKHAARPLLKIKLGTADDMARLEAVRAGAPKAAIIVDANEGWTAEIYTDLAP
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHH
HLLRLGVQMVEQPLPAGQDDMLSEIARPLPVCADESCHDRASLPDLKGKYDMVNIKLDKT
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
GGLSEALALRDAARAEGYTVMVGCMVGSSLAMAPAMLVAQGAEIVDLDGPLLLAEDRTPP
CCHHHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHHCCCEEEECCCCEEEECCCCCC
LKFDGSTIHPPEAALWG
EEECCCCCCCCCCCCCC
>Mature Secondary Structure
MFKITRDKFKLDEVFTISRGSKTHAEVLTFEIDQGASGRGRGECVPYARYGETLDSVEAQ
CCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCHHHHHHHH
IQDYIDNGDSARFPPGAARNAIECAILDAEAKCNATTVWEMLRQPVPQPVTSAFTLSLDT
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCC
PEKMRASAAKHAARPLLKIKLGTADDMARLEAVRAGAPKAAIIVDANEGWTAEIYTDLAP
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHH
HLLRLGVQMVEQPLPAGQDDMLSEIARPLPVCADESCHDRASLPDLKGKYDMVNIKLDKT
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
GGLSEALALRDAARAEGYTVMVGCMVGSSLAMAPAMLVAQGAEIVDLDGPLLLAEDRTPP
CCHHHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHHCCCEEEECCCCEEEECCCCCC
LKFDGSTIHPPEAALWG
EEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]