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The map label for this gene is nodL [H]

Identifier: 89052846

GI number: 89052846

Start: 358833

End: 359387

Strand: Reverse

Name: nodL [H]

Synonym: Jann_0355

Alternate gene names: 89052846

Gene position: 359387-358833 (Counterclockwise)

Preceding gene: 89052849

Following gene: 89052844

Centisome position: 8.32

GC content: 57.3

Gene sequence:

>555_bases
ATGAAAACAGAACGACAGAAGATGGACGCGGGCAAATGGTACACATGCCTTGATGATGAGCTTGAAGCGCTTCGTGTTAA
GGCGCGTGCTGCGGTGCATGAACACAATACTCTGCCGCCGGATGCCCGAGGTGCGATGGGACCAAAACTCGCGCAACTTG
TCAGGACTGGTGCGGCAATCATTGAGGCACCGTTCCACTGTGCATACGGCATCAATATCGTGCTTGGTGAGCAGGTTTTT
CTGAATGCAGGTTGCACAATCCTGGATTGCGCCAAGGTGACGATCAGCGACCAGTGTCAGCTTGGGCCGAACGTGCAGAT
CTATTGTGCCGAACACCATAAGGACCCGATCAAGCGAAGCGTCGAGGGGTTGGAGATCGCCCGTCCAGTCTTCATCGGGA
AGCAAGCCTGGATTGGTGGGGGAGCGATCATTCTGGCCGGTGTTACAATTGGGGCCGGTGCAGTCGTCGGGGCGGGCAGC
GTCGTCACGAAGGATGTTGGTCCCGGCGCGGTTGTGGTCGGCAATCCAGCCCGACCCGTTGGTCGAGCCGAATAA

Upstream 100 bases:

>100_bases
AATTGTTCAGGTATGCTCCATAGGGCGGACTCCAAAGAACAAGGATAAACATTTTCCGCAGCGCTTGTGCGACACCCGTT
AGAGACAAACCGGAGACGTC

Downstream 100 bases:

>100_bases
TCACGCACGACGCCTTTGCATGCCACACTATCCCGCGAAGTACTCTGTCAGCACGCGTCCATAGACCCTCTTCAGGTCCT
CGATATCGCTGATCCGCACG

Product: transferase hexapeptide protein

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKTERQKMDAGKWYTCLDDELEALRVKARAAVHEHNTLPPDARGAMGPKLAQLVRTGAAIIEAPFHCAYGINIVLGEQVF
LNAGCTILDCAKVTISDQCQLGPNVQIYCAEHHKDPIKRSVEGLEIARPVFIGKQAWIGGGAIILAGVTIGAGAVVGAGS
VVTKDVGPGAVVVGNPARPVGRAE

Sequences:

>Translated_184_residues
MKTERQKMDAGKWYTCLDDELEALRVKARAAVHEHNTLPPDARGAMGPKLAQLVRTGAAIIEAPFHCAYGINIVLGEQVF
LNAGCTILDCAKVTISDQCQLGPNVQIYCAEHHKDPIKRSVEGLEIARPVFIGKQAWIGGGAIILAGVTIGAGAVVGAGS
VVTKDVGPGAVVVGNPARPVGRAE
>Mature_184_residues
MKTERQKMDAGKWYTCLDDELEALRVKARAAVHEHNTLPPDARGAMGPKLAQLVRTGAAIIEAPFHCAYGINIVLGEQVF
LNAGCTILDCAKVTISDQCQLGPNVQIYCAEHHKDPIKRSVEGLEIARPVFIGKQAWIGGGAIILAGVTIGAGAVVGAGS
VVTKDVGPGAVVVGNPARPVGRAE

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=183, Percent_Identity=43.7158469945355, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI1786537, Length=177, Percent_Identity=32.7683615819209, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322243, Length=167, Percent_Identity=43.1137724550898, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 19286; Mature: 19286

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTERQKMDAGKWYTCLDDELEALRVKARAAVHEHNTLPPDARGAMGPKLAQLVRTGAAI
CCCCHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHH
IEAPFHCAYGINIVLGEQVFLNAGCTILDCAKVTISDQCQLGPNVQIYCAEHHKDPIKRS
HCCCCEEECCCEEEECCEEEECCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHH
VEGLEIARPVFIGKQAWIGGGAIILAGVTIGAGAVVGAGSVVTKDVGPGAVVVGNPARPV
HCCHHHHCCEEECCCCEECCCEEEEEEEEECCCCEECCCCEEEECCCCCEEEECCCCCCC
GRAE
CCCC
>Mature Secondary Structure
MKTERQKMDAGKWYTCLDDELEALRVKARAAVHEHNTLPPDARGAMGPKLAQLVRTGAAI
CCCCHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHH
IEAPFHCAYGINIVLGEQVFLNAGCTILDCAKVTISDQCQLGPNVQIYCAEHHKDPIKRS
HCCCCEEECCCEEEECCEEEECCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHH
VEGLEIARPVFIGKQAWIGGGAIILAGVTIGAGAVVGAGSVVTKDVGPGAVVVGNPARPV
HCCHHHHCCEEECCCCEECCCEEEEEEEEECCCCEECCCCEEEECCCCCEEEECCCCCCC
GRAE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 40 {triethanolamine}} 90 {maltose}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1558960; 8801423; 11481432 [H]