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The map label for this gene is dapE

Identifier: 89052845

GI number: 89052845

Start: 357639

End: 358805

Strand: Direct

Name: dapE

Synonym: Jann_0354

Alternate gene names: 89052845

Gene position: 357639-358805 (Clockwise)

Preceding gene: 89052842

Following gene: 89052847

Centisome position: 8.28

GC content: 64.61

Gene sequence:

>1167_bases
ATGTCACAAATTCCGACCCATGCCCCCGTCGATCCGGTGGCCCTCACGGCCGATCTGATCCGCTGCAACTCGGTCACGCC
GCTGGAAGGCGGGGCGCTGGTGTTGCTGGAGGCGCTGTTGTCCGATGCCGGGTTTGACTGCACGCGGGTGGATCGGGGCG
GTATCGCGAACCTCTATGCGCGTTGGGGCACGCGCGGCAACGGCAAGGCGTTTGGCTTCAACGGCCACACCGATGTGGTG
CCCGTGGGGGATGAAGCGGCCTGGACCCACCCCCCGTTTGGCGCGGAGGTCGACGGCGACTGGATGTACGGGCGGGGCGC
CACCGATATGAAATCGGGCGTCGCCGCTTTTGCCGCCGCCGCCATCGACTTCGTGCGTGACACACCTCCCGACGGGGCCA
TCGTTCTGGCCATCACCGGCGATGAGGAGGGCGAAAGCACCGACGGCACCATAGCGCTGCTGGACTGGATGGAGGAGGTG
GGCGAGCGCATCGACGTCTGCATCGTGGGAGAGCCGACCTGCCCCGAGCAGATGGGCGACATGATGAAGATCGGCCGCCG
CGGCGCGCTGACCGCGTTTTTTGAGGTCATCGGCAAACAGGGCCATTCCGCCTATCTTCACAAGGTGATCAATCCGATGC
CTGCCGTGGCGCTTCTGGGCCATCGCCTGTCCACCCATACGCTGGATGAGGGGACGGATCATTTCGATCCCTCTACCTGC
GCCATCACCACCATCGACACAGGCAACCCCGCCAACAACGTGATCCCCGCAAAGACGCGCATGACGGTAAACCTGCGGTT
CAACGATCTGCATTCCGGCGACAGCCTCACCGCGTGGCTGCGGGAGGAGGCCGACCGCGTCGCCGAGGACACCGGCACAA
CGATCCGGATGACCACCAAACTCAGCGGCGAGTCGTTCCTGACGCCCCCCGGTGACCTCTCCGATCTGGTGGGCAAAGCC
GTTCAGGCTGAAACGGGCCGCCAGCCCACCTTGTCGACCACCGGCGGCACATCCGATGCGCGCTTCGTGAAAAACCATTG
CCCCGTCGTGGAATTCGGCCTCGTTGGCCACCGCATGCACCAGGTCGATGAACGCGTGCGGATCAGCGATATCGAGGACC
TGAAGAGGGTCTATGGACGCGTGCTGACAGAGTACTTCGCGGGATAG

Upstream 100 bases:

>100_bases
GCAGCCTTTCCGGGCGGGTGTGCCATCTTGGTGCCACATTGTGATCCATCGGCCACGCCGTTAGGGTCGGACCCACCACA
TCCTGTATCCCGAGGCTTTC

Downstream 100 bases:

>100_bases
TGTGGCATGCAAAGGCGTCGTGCGTGATTATTCGGCTCGACCAACGGGTCGGGCTGGATTGCCGACCACAACCGCGCCGG
GACCAACATCCTTCGTGACG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSQIPTHAPVDPVALTADLIRCNSVTPLEGGALVLLEALLSDAGFDCTRVDRGGIANLYARWGTRGNGKAFGFNGHTDVV
PVGDEAAWTHPPFGAEVDGDWMYGRGATDMKSGVAAFAAAAIDFVRDTPPDGAIVLAITGDEEGESTDGTIALLDWMEEV
GERIDVCIVGEPTCPEQMGDMMKIGRRGALTAFFEVIGKQGHSAYLHKVINPMPAVALLGHRLSTHTLDEGTDHFDPSTC
AITTIDTGNPANNVIPAKTRMTVNLRFNDLHSGDSLTAWLREEADRVAEDTGTTIRMTTKLSGESFLTPPGDLSDLVGKA
VQAETGRQPTLSTTGGTSDARFVKNHCPVVEFGLVGHRMHQVDERVRISDIEDLKRVYGRVLTEYFAG

Sequences:

>Translated_388_residues
MSQIPTHAPVDPVALTADLIRCNSVTPLEGGALVLLEALLSDAGFDCTRVDRGGIANLYARWGTRGNGKAFGFNGHTDVV
PVGDEAAWTHPPFGAEVDGDWMYGRGATDMKSGVAAFAAAAIDFVRDTPPDGAIVLAITGDEEGESTDGTIALLDWMEEV
GERIDVCIVGEPTCPEQMGDMMKIGRRGALTAFFEVIGKQGHSAYLHKVINPMPAVALLGHRLSTHTLDEGTDHFDPSTC
AITTIDTGNPANNVIPAKTRMTVNLRFNDLHSGDSLTAWLREEADRVAEDTGTTIRMTTKLSGESFLTPPGDLSDLVGKA
VQAETGRQPTLSTTGGTSDARFVKNHCPVVEFGLVGHRMHQVDERVRISDIEDLKRVYGRVLTEYFAG
>Mature_387_residues
SQIPTHAPVDPVALTADLIRCNSVTPLEGGALVLLEALLSDAGFDCTRVDRGGIANLYARWGTRGNGKAFGFNGHTDVVP
VGDEAAWTHPPFGAEVDGDWMYGRGATDMKSGVAAFAAAAIDFVRDTPPDGAIVLAITGDEEGESTDGTIALLDWMEEVG
ERIDVCIVGEPTCPEQMGDMMKIGRRGALTAFFEVIGKQGHSAYLHKVINPMPAVALLGHRLSTHTLDEGTDHFDPSTCA
ITTIDTGNPANNVIPAKTRMTVNLRFNDLHSGDSLTAWLREEADRVAEDTGTTIRMTTKLSGESFLTPPGDLSDLVGKAV
QAETGRQPTLSTTGGTSDARFVKNHCPVVEFGLVGHRMHQVDERVRISDIEDLKRVYGRVLTEYFAG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=379, Percent_Identity=36.9393139841689, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790395, Length=369, Percent_Identity=24.390243902439, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_JANSC (Q28VJ1)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508296.1
- ProteinModelPortal:   Q28VJ1
- SMR:   Q28VJ1
- STRING:   Q28VJ1
- MEROPS:   M20.010
- GeneID:   3932795
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0354
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   Q28VJ1
- ProtClustDB:   PRK13009
- BioCyc:   JSP290400:JANN_0354-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41590; Mature: 41459

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 78-78 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIPTHAPVDPVALTADLIRCNSVTPLEGGALVLLEALLSDAGFDCTRVDRGGIANLYA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHH
RWGTRGNGKAFGFNGHTDVVPVGDEAAWTHPPFGAEVDGDWMYGRGATDMKSGVAAFAAA
HCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHH
AIDFVRDTPPDGAIVLAITGDEEGESTDGTIALLDWMEEVGERIDVCIVGEPTCPEQMGD
HHHHHHCCCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHCCEEEEEECCCCCHHHHHH
MMKIGRRGALTAFFEVIGKQGHSAYLHKVINPMPAVALLGHRLSTHTLDEGTDHFDPSTC
HHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
AITTIDTGNPANNVIPAKTRMTVNLRFNDLHSGDSLTAWLREEADRVAEDTGTTIRMTTK
EEEEEECCCCCCCEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
LSGESFLTPPGDLSDLVGKAVQAETGRQPTLSTTGGTSDARFVKNHCPVVEFGLVGHRMH
ECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHCCCEEEEHHHHHHHH
QVDERVRISDIEDLKRVYGRVLTEYFAG
HHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQIPTHAPVDPVALTADLIRCNSVTPLEGGALVLLEALLSDAGFDCTRVDRGGIANLYA
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHH
RWGTRGNGKAFGFNGHTDVVPVGDEAAWTHPPFGAEVDGDWMYGRGATDMKSGVAAFAAA
HCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHH
AIDFVRDTPPDGAIVLAITGDEEGESTDGTIALLDWMEEVGERIDVCIVGEPTCPEQMGD
HHHHHHCCCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHCCEEEEEECCCCCHHHHHH
MMKIGRRGALTAFFEVIGKQGHSAYLHKVINPMPAVALLGHRLSTHTLDEGTDHFDPSTC
HHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
AITTIDTGNPANNVIPAKTRMTVNLRFNDLHSGDSLTAWLREEADRVAEDTGTTIRMTTK
EEEEEECCCCCCCEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
LSGESFLTPPGDLSDLVGKAVQAETGRQPTLSTTGGTSDARFVKNHCPVVEFGLVGHRMH
ECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHCCCEEEEHHHHHHHH
QVDERVRISDIEDLKRVYGRVLTEYFAG
HHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA