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The map label for this gene is gntR [H]

Identifier: 89052802

GI number: 89052802

Start: 318540

End: 319562

Strand: Direct

Name: gntR [H]

Synonym: Jann_0311

Alternate gene names: 89052802

Gene position: 318540-319562 (Clockwise)

Preceding gene: 89052801

Following gene: 89052804

Centisome position: 7.38

GC content: 61.09

Gene sequence:

>1023_bases
TTGCGAGGACGAAATCCATTTGACGGCAAGCCGCAGATGACGCTGCGGGATGTGGCCGATGCGGCCTCGGTCAGTGAAAT
GACGGTCAGCCGGGTTCTGCGGAACCGGGGCGATGTCTCTGACAAAACGCGCGAGCGGGTATTCGAGGCGGCGCGGACGT
TGGGCTATGTGCCCAACAAGATCGCGGGTGCGCTGGCGTCGAACCGTGTGAACCTGGTGGGCGTGGTGATCCCGTCCCTG
TCGAATATGGTATTTCCTGACGTCCTCCACGGTATCGGCGCGGCACTGGATGGGACGGAATTACAGCCGGTCGTTGGAAC
CTCAAAATACGATCTTCAGCAGGAAGAGAAGGTCATTTACGAGATGCTAAGCTGGCGCCCCTCCGGCCTGATCGTCGCGG
GGTTGGAACATACGGACTCGGCCCGGGCGATGATGCGCAACGCGGGCGTACCGGTGGTCGAGGTCATGGACGTCGATGGC
GATCCGGTGGCCGCCGCTGTGGGCATCAGCCACCAGGCGGCGGGACGCGCGATGGCGGACGAGATCCTGGCGCGGGGCTA
CACGAATATCGGATATCTGGGGTCGTCCTCCACAGCCGATGCGCGGGCGCAGAAGCGGTTTCGTGGGTTTGAGGCTGGCA
TGGCAGAGGCAGGCGTGACCCTTGCGGACACGATTTTCTACGAGGGAACGTCGGGCTTTGGCACGGGTCGTAAGATGACC
CAGGCGCTATTGGAGCGAACGCCCGATATAGATTTTCTATACTATAATACGGATATGAACGCCGCCGGGGGCCTATTGTA
TTGCCTTGAAAAAGGCATGGATATCCCGGGGGAGATTGGCCTGGCCGGGTTCAATTCCTTTGAAGTTCTGGATGGTTTGC
CAATGCGGATCGCCACTATGGACTCGCAGCGGGAGGCCATCGGACGCCGCGCGGCAGAACTCATGATCGCCAATGAGTTG
ACCGAAGAGGTCGTGCAGTTGGAGCCGCAGTTCCTGCCCGGCGACACCGTGCGGGACCGCTGA

Upstream 100 bases:

>100_bases
ACGTTTGTCGATGATTTGGCCCGGTGCCGCAAGCGCTTGCGCAGGGGTGGTGCGCGCGCTTATCAAGATGGTGTAGCGCT
CTCGACCGAAGGACACTCCG

Downstream 100 bases:

>100_bases
ACCTGACATCTGGCAGGAGCGTTCTGACAACGGCCAGACTGCCCCCCATAGCGGCGTCGACGTCGATAACATAGTGGCCC
GCCAGACCCGCGGATCGTGG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRGRNPFDGKPQMTLRDVADAASVSEMTVSRVLRNRGDVSDKTRERVFEAARTLGYVPNKIAGALASNRVNLVGVVIPSL
SNMVFPDVLHGIGAALDGTELQPVVGTSKYDLQQEEKVIYEMLSWRPSGLIVAGLEHTDSARAMMRNAGVPVVEVMDVDG
DPVAAAVGISHQAAGRAMADEILARGYTNIGYLGSSSTADARAQKRFRGFEAGMAEAGVTLADTIFYEGTSGFGTGRKMT
QALLERTPDIDFLYYNTDMNAAGGLLYCLEKGMDIPGEIGLAGFNSFEVLDGLPMRIATMDSQREAIGRRAAELMIANEL
TEEVVQLEPQFLPGDTVRDR

Sequences:

>Translated_340_residues
MRGRNPFDGKPQMTLRDVADAASVSEMTVSRVLRNRGDVSDKTRERVFEAARTLGYVPNKIAGALASNRVNLVGVVIPSL
SNMVFPDVLHGIGAALDGTELQPVVGTSKYDLQQEEKVIYEMLSWRPSGLIVAGLEHTDSARAMMRNAGVPVVEVMDVDG
DPVAAAVGISHQAAGRAMADEILARGYTNIGYLGSSSTADARAQKRFRGFEAGMAEAGVTLADTIFYEGTSGFGTGRKMT
QALLERTPDIDFLYYNTDMNAAGGLLYCLEKGMDIPGEIGLAGFNSFEVLDGLPMRIATMDSQREAIGRRAAELMIANEL
TEEVVQLEPQFLPGDTVRDR
>Mature_340_residues
MRGRNPFDGKPQMTLRDVADAASVSEMTVSRVLRNRGDVSDKTRERVFEAARTLGYVPNKIAGALASNRVNLVGVVIPSL
SNMVFPDVLHGIGAALDGTELQPVVGTSKYDLQQEEKVIYEMLSWRPSGLIVAGLEHTDSARAMMRNAGVPVVEVMDVDG
DPVAAAVGISHQAAGRAMADEILARGYTNIGYLGSSSTADARAQKRFRGFEAGMAEAGVTLADTIFYEGTSGFGTGRKMT
QALLERTPDIDFLYYNTDMNAAGGLLYCLEKGMDIPGEIGLAGFNSFEVLDGLPMRIATMDSQREAIGRRAAELMIANEL
TEEVVQLEPQFLPGDTVRDR

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=332, Percent_Identity=31.9277108433735, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=29.9093655589124, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=24.0384615384615, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=26.643598615917, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788474, Length=304, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787580, Length=312, Percent_Identity=25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789068, Length=279, Percent_Identity=23.2974910394265, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=23.1454005934718, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=23.4567901234568, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787906, Length=306, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786540, Length=283, Percent_Identity=27.5618374558304, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=22.6837060702875, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36659; Mature: 36659

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRNPFDGKPQMTLRDVADAASVSEMTVSRVLRNRGDVSDKTRERVFEAARTLGYVPNK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHH
IAGALASNRVNLVGVVIPSLSNMVFPDVLHGIGAALDGTELQPVVGTSKYDLQQEEKVIY
HHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCHHHHHHHHH
EMLSWRPSGLIVAGLEHTDSARAMMRNAGVPVVEVMDVDGDPVAAAVGISHQAAGRAMAD
HHHCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHH
EILARGYTNIGYLGSSSTADARAQKRFRGFEAGMAEAGVTLADTIFYEGTSGFGTGRKMT
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCHHHCCCEEEHHEEECCCCCCCCHHHHH
QALLERTPDIDFLYYNTDMNAAGGLLYCLEKGMDIPGEIGLAGFNSFEVLDGLPMRIATM
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEC
DSQREAIGRRAAELMIANELTEEVVQLEPQFLPGDTVRDR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRGRNPFDGKPQMTLRDVADAASVSEMTVSRVLRNRGDVSDKTRERVFEAARTLGYVPNK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHH
IAGALASNRVNLVGVVIPSLSNMVFPDVLHGIGAALDGTELQPVVGTSKYDLQQEEKVIY
HHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCHHHHHHHHH
EMLSWRPSGLIVAGLEHTDSARAMMRNAGVPVVEVMDVDGDPVAAAVGISHQAAGRAMAD
HHHCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHH
EILARGYTNIGYLGSSSTADARAQKRFRGFEAGMAEAGVTLADTIFYEGTSGFGTGRKMT
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCHHHCCCEEEHHEEECCCCCCCCHHHHH
QALLERTPDIDFLYYNTDMNAAGGLLYCLEKGMDIPGEIGLAGFNSFEVLDGLPMRIATM
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEC
DSQREAIGRRAAELMIANELTEEVVQLEPQFLPGDTVRDR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]