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The map label for this gene is kefA [C]

Identifier: 89052627

GI number: 89052627

Start: 126057

End: 127412

Strand: Direct

Name: kefA [C]

Synonym: Jann_0136

Alternate gene names: 89052627

Gene position: 126057-127412 (Clockwise)

Preceding gene: 89052625

Following gene: 89052629

Centisome position: 2.92

GC content: 60.03

Gene sequence:

>1356_bases
ATGGACCCCGAAGTCGCCCCCGAAGACATCCCGGAAGACATCGTTGATGCCGAAGCGGAAGCATTTGACGCCGCCTTGAC
GGAAATTACGGGTCTGATGGGCCAGATAGAGACGTTTCTGGCTTCGCTCTTGCGCACCTGGAATGCCTATCAGATCGGGA
TCGTCATCGCGCTTCTGGCGGCCGCCTGGCTGATGAAACGCGCCTTTGGGCCGCGTATCCGGACCTGGATGGCCTCACGG
GAGGGTTGGCCAAAATGGCGCATGCGTGTCCTGGCGATCATCCATCGTCGCCTGTTCATCATCTTTTTCGTCCTTCTGAT
CTGGACCTTCGTCTTGATCATGCGGGAGGTCACCTGGCCATCGCGCTCCTTCCTGCTGACCATCGTGGCGGAACTGGCGA
CTGCATGGCTCTTCGTGGTCTTCGTTACACGGCTCATCCGCTCTGCCTTCCTGCGCGGCATCATACGCTACGGGGCGTGG
ATCTTCGTGACGCTGGTGATCCTGGATCTCACGGATGAGGCCGCGGCACTGCTGGATTCCGTGGGCTTCAATCTGGGCGA
TGCGCGGCTGTCGCTGCTAACACTTGTGCAGGCGATCCTCGTGGTCGCCGCCCTCGTGGTCATCGCGCGTTTTCTGACCA
GCACCGCCACCGGCCGCATCCAGAAGAACGAGGAAATCTCGCCGTCCATGCAGGTTCTGGCGGTCAAATTCCTGCAAGTC
GCCTTCTACGGCATTGCGATCTACATGGGGCTGCGGCTGACTGGTGTGGACCTGACGGGGCTTGCCGTCCTGTCCGGTGC
GATCGGCGTGGGCCTGGGTTTCGGCCTGCAGAAGGTGGTCTCCAATCTGGTGTCCGGCGTCATCATCCTGCTGGACAAGT
CGATCAAACCGGGTGACGTGATTTCGCTGGGGGAGACGTTCGGCTGGATCAACTCGCTTGGCGCGCGCTATGTCAGCGTC
GTGACCCGGGACGGGAAGGAATACCTGATCCCGAATGAGGATCTGATCACCAGCCAGGTGGTCAACTGGTCCCATTCCAA
CGAATTCGTGCGCCTCGATATCTATTTCGGCACAGCCTACGGCGATGACCCCCACATCGTGCGCAAACTGGCGATTGAGG
CGGCCCAGGGCGTCGAACGTGTCCTCAGCCACCGGGCACCGGTTTGTCACATCGTGGGCTTCGGAGACAGCTCCGTCGAC
TATATCCTGCGGTTCTGGATCAAGGACCCGACGGGCGGGCTCACCAACATCCGCGGCAACGTCTACCTGGCGTTGTGGGA
TACGTTCAGCGCCAACAACATCTCCATCCCATTCCCGCAGCGAGAAATCAAGATGTTGGACAAACAGGAAGATTAG

Upstream 100 bases:

>100_bases
CACGCGTGAATTACACGGGCGGACGCGCCTTGCCAAGGGCGGCGCTTGCCCGCTTGCCCCCGAGACGTCTAGTCTAAGCC
CTGAGCAGTGAAGGACCGAA

Downstream 100 bases:

>100_bases
AGACTTTTGGTGGTGGCCAGCATCTTCGGATCAAGCGCCAGTTCGATGATGTGCAAGCCGCCCGTCAGGGCTTGCCGATA
GGCGTCTGCGAATTGAGCAT

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MDPEVAPEDIPEDIVDAEAEAFDAALTEITGLMGQIETFLASLLRTWNAYQIGIVIALLAAAWLMKRAFGPRIRTWMASR
EGWPKWRMRVLAIIHRRLFIIFFVLLIWTFVLIMREVTWPSRSFLLTIVAELATAWLFVVFVTRLIRSAFLRGIIRYGAW
IFVTLVILDLTDEAAALLDSVGFNLGDARLSLLTLVQAILVVAALVVIARFLTSTATGRIQKNEEISPSMQVLAVKFLQV
AFYGIAIYMGLRLTGVDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDVISLGETFGWINSLGARYVSV
VTRDGKEYLIPNEDLITSQVVNWSHSNEFVRLDIYFGTAYGDDPHIVRKLAIEAAQGVERVLSHRAPVCHIVGFGDSSVD
YILRFWIKDPTGGLTNIRGNVYLALWDTFSANNISIPFPQREIKMLDKQED

Sequences:

>Translated_451_residues
MDPEVAPEDIPEDIVDAEAEAFDAALTEITGLMGQIETFLASLLRTWNAYQIGIVIALLAAAWLMKRAFGPRIRTWMASR
EGWPKWRMRVLAIIHRRLFIIFFVLLIWTFVLIMREVTWPSRSFLLTIVAELATAWLFVVFVTRLIRSAFLRGIIRYGAW
IFVTLVILDLTDEAAALLDSVGFNLGDARLSLLTLVQAILVVAALVVIARFLTSTATGRIQKNEEISPSMQVLAVKFLQV
AFYGIAIYMGLRLTGVDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDVISLGETFGWINSLGARYVSV
VTRDGKEYLIPNEDLITSQVVNWSHSNEFVRLDIYFGTAYGDDPHIVRKLAIEAAQGVERVLSHRAPVCHIVGFGDSSVD
YILRFWIKDPTGGLTNIRGNVYLALWDTFSANNISIPFPQREIKMLDKQED
>Mature_451_residues
MDPEVAPEDIPEDIVDAEAEAFDAALTEITGLMGQIETFLASLLRTWNAYQIGIVIALLAAAWLMKRAFGPRIRTWMASR
EGWPKWRMRVLAIIHRRLFIIFFVLLIWTFVLIMREVTWPSRSFLLTIVAELATAWLFVVFVTRLIRSAFLRGIIRYGAW
IFVTLVILDLTDEAAALLDSVGFNLGDARLSLLTLVQAILVVAALVVIARFLTSTATGRIQKNEEISPSMQVLAVKFLQV
AFYGIAIYMGLRLTGVDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDVISLGETFGWINSLGARYVSV
VTRDGKEYLIPNEDLITSQVVNWSHSNEFVRLDIYFGTAYGDDPHIVRKLAIEAAQGVERVLSHRAPVCHIVGFGDSSVD
YILRFWIKDPTGGLTNIRGNVYLALWDTFSANNISIPFPQREIKMLDKQED

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=197, Percent_Identity=32.48730964467, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI2367355, Length=197, Percent_Identity=28.4263959390863, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789291, Length=269, Percent_Identity=27.1375464684015, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 50132; Mature: 50132

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPEVAPEDIPEDIVDAEAEAFDAALTEITGLMGQIETFLASLLRTWNAYQIGIVIALLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAWLMKRAFGPRIRTWMASREGWPKWRMRVLAIIHRRLFIIFFVLLIWTFVLIMREVTWP
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRSFLLTIVAELATAWLFVVFVTRLIRSAFLRGIIRYGAWIFVTLVILDLTDEAAALLDS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VGFNLGDARLSLLTLVQAILVVAALVVIARFLTSTATGRIQKNEEISPSMQVLAVKFLQV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AFYGIAIYMGLRLTGVDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDV
HHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCE
ISLGETFGWINSLGARYVSVVTRDGKEYLIPNEDLITSQVVNWSHSNEFVRLDIYFGTAY
EEHHHHHHHHHHHHHHHHHHHEECCCEEECCCCHHHHHHHHCCCCCCCEEEEEEEEECCC
GDDPHIVRKLAIEAAQGVERVLSHRAPVCHIVGFGDSSVDYILRFWIKDPTGGLTNIRGN
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
VYLALWDTFSANNISIPFPQREIKMLDKQED
EEEEEEECCCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MDPEVAPEDIPEDIVDAEAEAFDAALTEITGLMGQIETFLASLLRTWNAYQIGIVIALLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAWLMKRAFGPRIRTWMASREGWPKWRMRVLAIIHRRLFIIFFVLLIWTFVLIMREVTWP
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRSFLLTIVAELATAWLFVVFVTRLIRSAFLRGIIRYGAWIFVTLVILDLTDEAAALLDS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VGFNLGDARLSLLTLVQAILVVAALVVIARFLTSTATGRIQKNEEISPSMQVLAVKFLQV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AFYGIAIYMGLRLTGVDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDV
HHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCE
ISLGETFGWINSLGARYVSVVTRDGKEYLIPNEDLITSQVVNWSHSNEFVRLDIYFGTAY
EEHHHHHHHHHHHHHHHHHHHEECCCEEECCCCHHHHHHHHCCCCCCCEEEEEEEEECCC
GDDPHIVRKLAIEAAQGVERVLSHRAPVCHIVGFGDSSVDYILRFWIKDPTGGLTNIRGN
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
VYLALWDTFSANNISIPFPQREIKMLDKQED
EEEEEEECCCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]