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The map label for this gene is dagA [H]

Identifier: 89052511

GI number: 89052511

Start: 19046

End: 20602

Strand: Reverse

Name: dagA [H]

Synonym: Jann_0020

Alternate gene names: 89052511

Gene position: 20602-19046 (Counterclockwise)

Preceding gene: 89052512

Following gene: 89052510

Centisome position: 0.48

GC content: 60.31

Gene sequence:

>1557_bases
GTGAGAAACCTATACTCCATTCTGTTTGCAATCGCGGCCTATGTGGCCGTGTCCCTGCCTGCTGCGGCTCAGGAGGCAGC
GGCGACACTCGACGAACGGGTCAACGCAGCCTTCGCCAACGCCACGGGCTGGTTCGTGAATTTCATCTTCATGTCGATCC
CCGGCACCAACTTCCCCTGGATCGTCATGTGGCTGGTGATCGGCGCGACGGTTTTTACCATCTACTTCGCTGCTGTGCAG
TTTCGGTTCTTCGGCCATGCCATCGGGCTGGTGAAGGGCGATTACTCCGATCCCAATGATGCGGGCGAGGTCAGCCACTT
TCAGGCGCTGGCCACGGCTCTGTCGGGCACGGTCGGCCTTGGGAACATCGCGGGCGTCGCGGTTGCCGTTGGTATCGGTG
GACCGGGCGCCACGTTCTGGATGATCCTTGCGGGTCTTCTGGGCATGGCGTCGAAGTTCACCGAATGTACGTTGGGCGTG
AAGTACCGCAATGAGTACCCCGATGGCACCGTGTCGGGCGGACCGATGTACTACATCTCCAAGGGCTTCAACGAGCTGGG
CCTGCCGGGCGGCAAGATCCTCGCGGTCCTGTTTTCCATCTTCTGTATCCTCGGCGCGCTTGGCGGGGGCAACATGTTCC
AGGCCAATCAGGCCCACGCACAGATCACCCAGATCACCGGAGATTTCCCCGGCTGGATCACCGGCCTGATCTTCGCGGCG
GTCGTCTTCGCGGTCATCGTGGGTGGCATCAAGTCCATCGCCAAAGTGACGGAAAAGATCGTCCCGTTCATGGGCATCCT
CTACGTCGGTGCGGCGATCATCGTGCTGGTCGTCAACTACGACATGATCGGCTGGGCGTTTGGTCAGATCTTTGCCGGGG
CCTTCACGGGTCTGGGCGTCGCGGGCGGCTTCGTCGGCGCGTTGATCCAGGGCTTCAAGCGCGCGGCCTTCTCCAACGAG
GCGGGCGTTGGCTCTGCGGCGATTGCCCACTCTGCGGTGAAAACCAAAGAACCGATCACCGAGGGCTTCGTGTCCTTGCT
GGAGCCGCTGATTGACACCGTGGTCATCTGCACGATGACGGCGCTGGTCATCATCATCTCTCAGCAGTTGATCATTGATG
AGGCATCGGGCCTTTACATGCTGAACGAGGCGGGAACGGCGATTGCCACAGTTGATGGCAACGGCGGTGTTGCCCTGACA
TCGGCGGCGTTTGGGTCAGCGATCAGCTGGTTCCCGTTCGTGCTGGCGATTGCGGTGATCCTCTTCGCCTTCTCCACCAT
GATCTCGTGGTCGTATTACGGGTTGAAGGCCTGGACGTATCTGTTCGGCGAAGGCAAGACGACGGAGCTGATCTTCAAGC
TCATCTTCTGCGCCTTCATCGTGATCGGAGCGGCGGCGTCGCTTGGGCCCGTCATCGACTTCTCGGATGCCGCGATCTTC
GCGATGGCGGTGGTCAACATCTTCTGCCTCTACTTCCTGATGAAGGTGGTGAAGGCGGAGTTGTCCTCCTACAGCGCGCG
CCTGAAGTCCGGTGAAATCAGGAAGTTCAGCCACTAA

Upstream 100 bases:

>100_bases
TGCCGGCGGGATCACGTCGCGGCTGTTCGGCAGCTCCACCGCAAATCTTGTGATGAACGCCCGCGTTCCTGTCACTGTCG
TCGCATAAGGATTTGGCCCC

Downstream 100 bases:

>100_bases
TACCGGCTGTCTGAACACGGGAGAGGCCGCTGGCGAAAGCTGCGGCCTCTGTCTTTTTTTGCAGATCAGGACTGGCGAAC
GCGTCCGAGCGCGATACTCA

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MRNLYSILFAIAAYVAVSLPAAAQEAAATLDERVNAAFANATGWFVNFIFMSIPGTNFPWIVMWLVIGATVFTIYFAAVQ
FRFFGHAIGLVKGDYSDPNDAGEVSHFQALATALSGTVGLGNIAGVAVAVGIGGPGATFWMILAGLLGMASKFTECTLGV
KYRNEYPDGTVSGGPMYYISKGFNELGLPGGKILAVLFSIFCILGALGGGNMFQANQAHAQITQITGDFPGWITGLIFAA
VVFAVIVGGIKSIAKVTEKIVPFMGILYVGAAIIVLVVNYDMIGWAFGQIFAGAFTGLGVAGGFVGALIQGFKRAAFSNE
AGVGSAAIAHSAVKTKEPITEGFVSLLEPLIDTVVICTMTALVIIISQQLIIDEASGLYMLNEAGTAIATVDGNGGVALT
SAAFGSAISWFPFVLAIAVILFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKLIFCAFIVIGAAASLGPVIDFSDAAIF
AMAVVNIFCLYFLMKVVKAELSSYSARLKSGEIRKFSH

Sequences:

>Translated_518_residues
MRNLYSILFAIAAYVAVSLPAAAQEAAATLDERVNAAFANATGWFVNFIFMSIPGTNFPWIVMWLVIGATVFTIYFAAVQ
FRFFGHAIGLVKGDYSDPNDAGEVSHFQALATALSGTVGLGNIAGVAVAVGIGGPGATFWMILAGLLGMASKFTECTLGV
KYRNEYPDGTVSGGPMYYISKGFNELGLPGGKILAVLFSIFCILGALGGGNMFQANQAHAQITQITGDFPGWITGLIFAA
VVFAVIVGGIKSIAKVTEKIVPFMGILYVGAAIIVLVVNYDMIGWAFGQIFAGAFTGLGVAGGFVGALIQGFKRAAFSNE
AGVGSAAIAHSAVKTKEPITEGFVSLLEPLIDTVVICTMTALVIIISQQLIIDEASGLYMLNEAGTAIATVDGNGGVALT
SAAFGSAISWFPFVLAIAVILFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKLIFCAFIVIGAAASLGPVIDFSDAAIF
AMAVVNIFCLYFLMKVVKAELSSYSARLKSGEIRKFSH
>Mature_518_residues
MRNLYSILFAIAAYVAVSLPAAAQEAAATLDERVNAAFANATGWFVNFIFMSIPGTNFPWIVMWLVIGATVFTIYFAAVQ
FRFFGHAIGLVKGDYSDPNDAGEVSHFQALATALSGTVGLGNIAGVAVAVGIGGPGATFWMILAGLLGMASKFTECTLGV
KYRNEYPDGTVSGGPMYYISKGFNELGLPGGKILAVLFSIFCILGALGGGNMFQANQAHAQITQITGDFPGWITGLIFAA
VVFAVIVGGIKSIAKVTEKIVPFMGILYVGAAIIVLVVNYDMIGWAFGQIFAGAFTGLGVAGGFVGALIQGFKRAAFSNE
AGVGSAAIAHSAVKTKEPITEGFVSLLEPLIDTVVICTMTALVIIISQQLIIDEASGLYMLNEAGTAIATVDGNGGVALT
SAAFGSAISWFPFVLAIAVILFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKLIFCAFIVIGAAASLGPVIDFSDAAIF
AMAVVNIFCLYFLMKVVKAELSSYSARLKSGEIRKFSH

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=467, Percent_Identity=31.4775160599572, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 54467; Mature: 54467

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLYSILFAIAAYVAVSLPAAAQEAAATLDERVNAAFANATGWFVNFIFMSIPGTNFPW
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHH
IVMWLVIGATVFTIYFAAVQFRFFGHAIGLVKGDYSDPNDAGEVSHFQALATALSGTVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
GNIAGVAVAVGIGGPGATFWMILAGLLGMASKFTECTLGVKYRNEYPDGTVSGGPMYYIS
CHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEE
KGFNELGLPGGKILAVLFSIFCILGALGGGNMFQANQAHAQITQITGDFPGWITGLIFAA
CCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
VVFAVIVGGIKSIAKVTEKIVPFMGILYVGAAIIVLVVNYDMIGWAFGQIFAGAFTGLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AGGFVGALIQGFKRAAFSNEAGVGSAAIAHSAVKTKEPITEGFVSLLEPLIDTVVICTMT
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ALVIIISQQLIIDEASGLYMLNEAGTAIATVDGNGGVALTSAAFGSAISWFPFVLAIAVI
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
LFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKLIFCAFIVIGAAASLGPVIDFSDAAIF
HHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHH
AMAVVNIFCLYFLMKVVKAELSSYSARLKSGEIRKFSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRNLYSILFAIAAYVAVSLPAAAQEAAATLDERVNAAFANATGWFVNFIFMSIPGTNFPW
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHH
IVMWLVIGATVFTIYFAAVQFRFFGHAIGLVKGDYSDPNDAGEVSHFQALATALSGTVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
GNIAGVAVAVGIGGPGATFWMILAGLLGMASKFTECTLGVKYRNEYPDGTVSGGPMYYIS
CHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEE
KGFNELGLPGGKILAVLFSIFCILGALGGGNMFQANQAHAQITQITGDFPGWITGLIFAA
CCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
VVFAVIVGGIKSIAKVTEKIVPFMGILYVGAAIIVLVVNYDMIGWAFGQIFAGAFTGLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AGGFVGALIQGFKRAAFSNEAGVGSAAIAHSAVKTKEPITEGFVSLLEPLIDTVVICTMT
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ALVIIISQQLIIDEASGLYMLNEAGTAIATVDGNGGVALTSAAFGSAISWFPFVLAIAVI
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
LFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKLIFCAFIVIGAAASLGPVIDFSDAAIF
HHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHH
AMAVVNIFCLYFLMKVVKAELSSYSARLKSGEIRKFSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]