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The map label for this gene is trmE

Identifier: 88658612

GI number: 88658612

Start: 52852

End: 54171

Strand: Direct

Name: trmE

Synonym: ECH_0060

Alternate gene names: 88658612

Gene position: 52852-54171 (Clockwise)

Preceding gene: 88658476

Following gene: 88658514

Centisome position: 4.49

GC content: 31.67

Gene sequence:

>1320_bases
ATGACTACAATTTTTGCTTTATGTACTCCATGGGGAAGGTCTGGAGTAGCTGTGATTAGAATCTCTGGTGAAGATGCTGC
AAAGGCATTTGTGCATTTTGGAATTAATAGTAGTATTAAACCAAGAACTGCAACGTTTACTCCTTTATACGACAAAGATG
GTGAAGTTATAGATGAAGCGATAGTGGTGTATTTTGTTGCACCAAATAGCTTTACTGGAGAAGATGTAGTTGAGTTTCAT
ACTCATGGTAGTTTTGCTGTTATTAAAATGATATTAGCTGAGCTTGGAAAAATTTTTGTTCCTGCTGGGCCAGGTGAGTT
TTCTTTGCGTGCTTTTTTAAATAATAAAGTTGATTTGACTAGAGCTGAAGCAATAGTAGATCTTATAAACTCTGAAACAG
AAATGCAGGCTAAGCAAGCTATAAGACAAATGTCTGGGGTTTTGGAAAAGTTGTATCAAAATTGGCGACAGCAGTTGATT
GATATATTATCTAATATAGAAGCTTATATTGATTTTCCGGAAGAAGTTAATAGTTCTGCTATAGCTAATATTGATTATCT
ATTAAATAATTTGCAGAAATCTTTAGAAAGTCATCTTAATGATGACCGAAGAGGTGAAAGGTTACGTCAAGGTATTTATG
TTACAATTCTTGGTGAACCTAATTCTGGTAAGTCTACTTTATTTAATCATTTAGCAAAAAGAGATATTGCAATTGTTTCT
GAATATGCTGGTACTACTAGAGATCCTCTAGAAGCACATATAGATGTAGCTGGATATCCAATTATTATTATTGATACTGC
AGGCATTAGAGAGAGTACTGATCCAGTAGAACAAGAAGGGATCAAACGTGCGAAGTTAAAGGCTGAAAATGCTGATTTCA
AAATAGTAATGCTTCCTTACGAGAAACGAGATATTTTTAACAGAGAAATTATGAGCTTGATAGATGATAAGTCTATATGC
ATTTTAAGTAAAGCTGATAATATTACAGATCAGAAATTAATACCTGTGTTTGATTTTAGTTTTATTCCGATTTCTGTATA
TTGTAACATTGGAATTGAAAATTTATTGAATTTAATTAAACAAAAAGTAGAAAAAGATTTTCAATTTTGTAATACTGACC
CTTTTATTACTTCTGAAAGGCAGAGAAAACATATTCAAAATACTTTGAATATTATAAAGTCTGTAGATTTAAGTTTACCT
ATGGAAATAGTGTCAGAGGATTTAAGATTGTCTGTAAGGGAATTAGGGAAAGTGGTAGGTGTTATTAGTGATGATGATAT
ATTAGATAATGTATTTGGTAAATTCTGTATAGGTAAATGA

Upstream 100 bases:

>100_bases
GCTTATGTTGTTTAATGGAATATAATTTTCTATTTAATAATATAGTGAGTTTTATTCTAATGATGATAAAATTCTAGAGT
ACTTCTACTATTTGGAATAA

Downstream 100 bases:

>100_bases
TATTTACCTGGATTATTAGTGGGCATGTGTGTAAGTTCTTGGATTCATAATTTTATTAATAAAATACATATAGTGATTTT
TTGTTGTGGAGTTATTTTGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTTIFALCTPWGRSGVAVIRISGEDAAKAFVHFGINSSIKPRTATFTPLYDKDGEVIDEAIVVYFVAPNSFTGEDVVEFH
THGSFAVIKMILAELGKIFVPAGPGEFSLRAFLNNKVDLTRAEAIVDLINSETEMQAKQAIRQMSGVLEKLYQNWRQQLI
DILSNIEAYIDFPEEVNSSAIANIDYLLNNLQKSLESHLNDDRRGERLRQGIYVTILGEPNSGKSTLFNHLAKRDIAIVS
EYAGTTRDPLEAHIDVAGYPIIIIDTAGIRESTDPVEQEGIKRAKLKAENADFKIVMLPYEKRDIFNREIMSLIDDKSIC
ILSKADNITDQKLIPVFDFSFIPISVYCNIGIENLLNLIKQKVEKDFQFCNTDPFITSERQRKHIQNTLNIIKSVDLSLP
MEIVSEDLRLSVRELGKVVGVISDDDILDNVFGKFCIGK

Sequences:

>Translated_439_residues
MTTIFALCTPWGRSGVAVIRISGEDAAKAFVHFGINSSIKPRTATFTPLYDKDGEVIDEAIVVYFVAPNSFTGEDVVEFH
THGSFAVIKMILAELGKIFVPAGPGEFSLRAFLNNKVDLTRAEAIVDLINSETEMQAKQAIRQMSGVLEKLYQNWRQQLI
DILSNIEAYIDFPEEVNSSAIANIDYLLNNLQKSLESHLNDDRRGERLRQGIYVTILGEPNSGKSTLFNHLAKRDIAIVS
EYAGTTRDPLEAHIDVAGYPIIIIDTAGIRESTDPVEQEGIKRAKLKAENADFKIVMLPYEKRDIFNREIMSLIDDKSIC
ILSKADNITDQKLIPVFDFSFIPISVYCNIGIENLLNLIKQKVEKDFQFCNTDPFITSERQRKHIQNTLNIIKSVDLSLP
MEIVSEDLRLSVRELGKVVGVISDDDILDNVFGKFCIGK
>Mature_438_residues
TTIFALCTPWGRSGVAVIRISGEDAAKAFVHFGINSSIKPRTATFTPLYDKDGEVIDEAIVVYFVAPNSFTGEDVVEFHT
HGSFAVIKMILAELGKIFVPAGPGEFSLRAFLNNKVDLTRAEAIVDLINSETEMQAKQAIRQMSGVLEKLYQNWRQQLID
ILSNIEAYIDFPEEVNSSAIANIDYLLNNLQKSLESHLNDDRRGERLRQGIYVTILGEPNSGKSTLFNHLAKRDIAIVSE
YAGTTRDPLEAHIDVAGYPIIIIDTAGIRESTDPVEQEGIKRAKLKAENADFKIVMLPYEKRDIFNREIMSLIDDKSICI
LSKADNITDQKLIPVFDFSFIPISVYCNIGIENLLNLIKQKVEKDFQFCNTDPFITSERQRKHIQNTLNIIKSVDLSLPM
EIVSEDLRLSVRELGKVVGVISDDDILDNVFGKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=461, Percent_Identity=36.6594360086768, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI193082993, Length=446, Percent_Identity=36.9955156950673, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI193082988, Length=493, Percent_Identity=34.2799188640974, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=36.3436123348018, Blast_Score=265, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17507259, Length=452, Percent_Identity=32.7433628318584, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=34.2857142857143, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=36.5546218487395, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_EHRCR (Q2GI42)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_506891.1
- ProteinModelPortal:   Q2GI42
- SMR:   Q2GI42
- STRING:   Q2GI42
- GeneID:   3927317
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0060
- TIGR:   ECH_0060
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- PhylomeDB:   Q2GI42
- ProtClustDB:   PRK05291
- BioCyc:   ECHA205920:ECH_0060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49224; Mature: 49093

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 78-78 BINDING 116-116 BINDING 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIFALCTPWGRSGVAVIRISGEDAAKAFVHFGINSSIKPRTATFTPLYDKDGEVIDEA
CCEEEEEECCCCCCCEEEEEECCCHHHHHHHEECCCCCCCCCCEEECCCCCCCCHHHCCE
IVVYFVAPNSFTGEDVVEFHTHGSFAVIKMILAELGKIFVPAGPGEFSLRAFLNNKVDLT
EEEEEECCCCCCCCCHHEEECCCCHHHHHHHHHHHCCEEECCCCCCHHHHHHHCCCCCHH
RAEAIVDLINSETEMQAKQAIRQMSGVLEKLYQNWRQQLIDILSNIEAYIDFPEEVNSSA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCHHHCCCHH
IANIDYLLNNLQKSLESHLNDDRRGERLRQGIYVTILGEPNSGKSTLFNHLAKRDIAIVS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EYAGTTRDPLEAHIDVAGYPIIIIDTAGIRESTDPVEQEGIKRAKLKAENADFKIVMLPY
HHCCCCCCCHHHEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECC
EKRDIFNREIMSLIDDKSICILSKADNITDQKLIPVFDFSFIPISVYCNIGIENLLNLIK
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCEEEEEEEEECCHHHHHHHHH
QKVEKDFQFCNTDPFITSERQRKHIQNTLNIIKSVDLSLPMEIVSEDLRLSVRELGKVVG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHE
VISDDDILDNVFGKFCIGK
ECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIFALCTPWGRSGVAVIRISGEDAAKAFVHFGINSSIKPRTATFTPLYDKDGEVIDEA
CEEEEEECCCCCCCEEEEEECCCHHHHHHHEECCCCCCCCCCEEECCCCCCCCHHHCCE
IVVYFVAPNSFTGEDVVEFHTHGSFAVIKMILAELGKIFVPAGPGEFSLRAFLNNKVDLT
EEEEEECCCCCCCCCHHEEECCCCHHHHHHHHHHHCCEEECCCCCCHHHHHHHCCCCCHH
RAEAIVDLINSETEMQAKQAIRQMSGVLEKLYQNWRQQLIDILSNIEAYIDFPEEVNSSA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCHHHCCCHH
IANIDYLLNNLQKSLESHLNDDRRGERLRQGIYVTILGEPNSGKSTLFNHLAKRDIAIVS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EYAGTTRDPLEAHIDVAGYPIIIIDTAGIRESTDPVEQEGIKRAKLKAENADFKIVMLPY
HHCCCCCCCHHHEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECC
EKRDIFNREIMSLIDDKSICILSKADNITDQKLIPVFDFSFIPISVYCNIGIENLLNLIK
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCEEEEEEEEECCHHHHHHHHH
QKVEKDFQFCNTDPFITSERQRKHIQNTLNIIKSVDLSLPMEIVSEDLRLSVRELGKVVG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHE
VISDDDILDNVFGKFCIGK
ECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA