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The map label for this gene is pyrF

Identifier: 88658602

GI number: 88658602

Start: 807257

End: 807952

Strand: Reverse

Name: pyrF

Synonym: ECH_0792

Alternate gene names: 88658602

Gene position: 807952-807257 (Counterclockwise)

Preceding gene: 88658121

Following gene: 88657950

Centisome position: 68.69

GC content: 34.63

Gene sequence:

>696_bases
ATGTTTACTAATCCGATTATTTGTGCATTGGATACTCATGATATCAACCATGCATTACTGTTGACTAAGATGCTTTATGG
AAGAGTATCGATGGTAAAGTTGGGACTAGAGTTTTTTACAGCGTATGGGTTATCTGGTGTACAAGCGATAGCTGATTGTG
GTGTTCCTATATTTCTTGATTTAAAATTACATGATATTCCTAATACGGTAAGTAAAGCTATATCAGTGATAGCATCTTTA
AATGTTGCTATGCTTACTATTCATGTTAGTGGTGGTAGGGAGATGATGATCAGAGCTATGGATAGCATATCTGGTAGTGT
AACAAAGTTGGTAGGTGTAACTGTATTAACGAGTATGGATGATTCTGATTTAAAGGAAATTGGTGTTAATGAATCACCTG
TTCAGCAAGTTATGCTTTTGTCTAAGTTAGCTAGAGAAGTTGGGTTATATGGTATAGTATGCTCTGCTTTTGAAGCAAAG
GAAGTGCGTAATCGATATACAGAAAAGGATTTAAAGCTTATAGTGCCAGGTATAAGATTTGATAGTGACTGTAATGATCA
AAAAAGGGTAAAGAGCCCTAAAGATGCAATGTTAGCTGGTGCAAATTATTTAGTAATTGGACGTCCAATTACCATGAGTA
GTGATCCTGTGCAAACAGTTGAAGATATTTTATTATCAATATCAAAATGTATATGA

Upstream 100 bases:

>100_bases
ATAATATGACGATGAGGTAATATATAATATTTATTCAAATTAATAAAGTTGTGTTAAATAGTTAATATTAGTATAATTTG
AATGTTTTTTAGGAATATTT

Downstream 100 bases:

>100_bases
GTTGCGAAATTGATAATGTTTTGTATCAAAGGTCATATGTTTTTGTTAAGTTATGAACAGTTAAAAGGTAGATATCATAA
TTTTTTAGGATAAAATGTTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MFTNPIICALDTHDINHALLLTKMLYGRVSMVKLGLEFFTAYGLSGVQAIADCGVPIFLDLKLHDIPNTVSKAISVIASL
NVAMLTIHVSGGREMMIRAMDSISGSVTKLVGVTVLTSMDDSDLKEIGVNESPVQQVMLLSKLAREVGLYGIVCSAFEAK
EVRNRYTEKDLKLIVPGIRFDSDCNDQKRVKSPKDAMLAGANYLVIGRPITMSSDPVQTVEDILLSISKCI

Sequences:

>Translated_231_residues
MFTNPIICALDTHDINHALLLTKMLYGRVSMVKLGLEFFTAYGLSGVQAIADCGVPIFLDLKLHDIPNTVSKAISVIASL
NVAMLTIHVSGGREMMIRAMDSISGSVTKLVGVTVLTSMDDSDLKEIGVNESPVQQVMLLSKLAREVGLYGIVCSAFEAK
EVRNRYTEKDLKLIVPGIRFDSDCNDQKRVKSPKDAMLAGANYLVIGRPITMSSDPVQTVEDILLSISKCI
>Mature_231_residues
MFTNPIICALDTHDINHALLLTKMLYGRVSMVKLGLEFFTAYGLSGVQAIADCGVPIFLDLKLHDIPNTVSKAISVIASL
NVAMLTIHVSGGREMMIRAMDSISGSVTKLVGVTVLTSMDDSDLKEIGVNESPVQQVMLLSKLAREVGLYGIVCSAFEAK
EVRNRYTEKDLKLIVPGIRFDSDCNDQKRVKSPKDAMLAGANYLVIGRPITMSSDPVQTVEDILLSISKCI

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=40.4347826086956, Blast_Score=166, Evalue=9e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_EHRCR (Q2GG43)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507590.1
- STRING:   Q2GG43
- GeneID:   3927934
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0792
- TIGR:   ECH_0792
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2GG43
- ProtClustDB:   CLSK837647
- BioCyc:   ECHA205920:ECH_0792-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25121; Mature: 25121

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00156 OMPDECASE; PS00599 AA_TRANSFER_CLASS_2

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 117-117 BINDING 179-179 BINDING 187-187 BINDING 207-207 BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTNPIICALDTHDINHALLLTKMLYGRVSMVKLGLEFFTAYGLSGVQAIADCGVPIFLD
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEE
LKLHDIPNTVSKAISVIASLNVAMLTIHVSGGREMMIRAMDSISGSVTKLVGVTVLTSMD
EEECCCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHEECCC
DSDLKEIGVNESPVQQVMLLSKLAREVGLYGIVCSAFEAKEVRNRYTEKDLKLIVPGIRF
CHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
DSDCNDQKRVKSPKDAMLAGANYLVIGRPITMSSDPVQTVEDILLSISKCI
CCCCCCHHHHCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTNPIICALDTHDINHALLLTKMLYGRVSMVKLGLEFFTAYGLSGVQAIADCGVPIFLD
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEE
LKLHDIPNTVSKAISVIASLNVAMLTIHVSGGREMMIRAMDSISGSVTKLVGVTVLTSMD
EEECCCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHEECCC
DSDLKEIGVNESPVQQVMLLSKLAREVGLYGIVCSAFEAKEVRNRYTEKDLKLIVPGIRF
CHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
DSDCNDQKRVKSPKDAMLAGANYLVIGRPITMSSDPVQTVEDILLSISKCI
CCCCCCHHHHCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA