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The map label for this gene is tsf

Identifier: 88658488

GI number: 88658488

Start: 515947

End: 516813

Strand: Direct

Name: tsf

Synonym: ECH_0515

Alternate gene names: 88658488

Gene position: 515947-516813 (Clockwise)

Preceding gene: 88657981

Following gene: 88657703

Centisome position: 43.86

GC content: 31.03

Gene sequence:

>867_bases
ATGAAAGTTGATATTAATGCAGTAAAAGAATTGCGTAATTTGACAGGTGCTGGTGTTGGTGATTGTAAGGAAGCATTAAA
TTCTTGTGGTGGTGACATAGAAAAAGCAAAAAATTATCTTAGGGAGCAAGGTATTGCAAAAGCTTACAAAAAATCTACTA
AGGATGTATCAGATGGTTTAGTAGCAGTTCATGTTAATGGAAACCAAGGCGCAATTTTAGAAGTGAATTCGGAAACTGAT
TTTGTTGCTCGTAATGAAAAATTTCAAAAATTGGTGTTAAATCTTGTTTCTTTAGCTAATCAGTATGCTGTTGAAGATAT
AGAAGATTTTTTAAAACATGAATATGTTAGTGGAACTAGTATTCATGATGAAATTATGACTAATATTGCAGTAATAGGAG
AAAATATTCATCTTAATAAAATAGGGTACTTATCTGTTAATGCTGGTGTTGTAGGTGGGTATATACATAGTCCTGTGGTT
AATAATCTTGGTAAAATAGGAGCAATAGTTGCTTTAGAGTCTACTGCTGATAATGACAAATTAAATGTACTTGCAAGACA
AATAGCTATGCATATTGTTGCTGCAAGACCAGAAGCATTATCTGTGGATCTTTTAGATAAAGATATTTTGGATAAGGAAC
GTGAAATTATAAAGAAGCAGGTCGACCAATTAAATAAACCAGTGTCAGTAGCAGAAAGAATAATAGATGGTAGAATAGCT
AAATTTTACCAAGATGTTGTTTTATTAGAGCAGATCTTTGTGATGGATAATCAATTAACAATTTCTGAATTAATAAAGAA
AAAAGAATCAGAATTGGGTGCAAGTATTAATCTTGTAGGGTATAAGCTATTTGTCATTAGTAAATAA

Upstream 100 bases:

>100_bases
TTGAGAGCAAATAATCGAGATCATAAAAACAATAAAAATAATAGTACTATTGATAATGCTGAAAATCTTAAGGAAGAAAA
TCTGGTAGGAGGAAGTAATA

Downstream 100 bases:

>100_bases
TGCTATAAAGGTTAGTATTTTTATAGTTTAAATTTGTAGGACATGGTAGGTATACTATCTTAGTTGTTTATGGCTTGTAT
ACAATTTTTATGTAGTTAGT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKVDINAVKELRNLTGAGVGDCKEALNSCGGDIEKAKNYLREQGIAKAYKKSTKDVSDGLVAVHVNGNQGAILEVNSETD
FVARNEKFQKLVLNLVSLANQYAVEDIEDFLKHEYVSGTSIHDEIMTNIAVIGENIHLNKIGYLSVNAGVVGGYIHSPVV
NNLGKIGAIVALESTADNDKLNVLARQIAMHIVAARPEALSVDLLDKDILDKEREIIKKQVDQLNKPVSVAERIIDGRIA
KFYQDVVLLEQIFVMDNQLTISELIKKKESELGASINLVGYKLFVISK

Sequences:

>Translated_288_residues
MKVDINAVKELRNLTGAGVGDCKEALNSCGGDIEKAKNYLREQGIAKAYKKSTKDVSDGLVAVHVNGNQGAILEVNSETD
FVARNEKFQKLVLNLVSLANQYAVEDIEDFLKHEYVSGTSIHDEIMTNIAVIGENIHLNKIGYLSVNAGVVGGYIHSPVV
NNLGKIGAIVALESTADNDKLNVLARQIAMHIVAARPEALSVDLLDKDILDKEREIIKKQVDQLNKPVSVAERIIDGRIA
KFYQDVVLLEQIFVMDNQLTISELIKKKESELGASINLVGYKLFVISK
>Mature_288_residues
MKVDINAVKELRNLTGAGVGDCKEALNSCGGDIEKAKNYLREQGIAKAYKKSTKDVSDGLVAVHVNGNQGAILEVNSETD
FVARNEKFQKLVLNLVSLANQYAVEDIEDFLKHEYVSGTSIHDEIMTNIAVIGENIHLNKIGYLSVNAGVVGGYIHSPVV
NNLGKIGAIVALESTADNDKLNVLARQIAMHIVAARPEALSVDLLDKDILDKEREIIKKQVDQLNKPVSVAERIIDGRIA
KFYQDVVLLEQIFVMDNQLTISELIKKKESELGASINLVGYKLFVISK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=226, Percent_Identity=33.1858407079646, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI291084500, Length=246, Percent_Identity=32.1138211382114, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI291084498, Length=104, Percent_Identity=37.5, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI291084502, Length=104, Percent_Identity=37.5, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786366, Length=274, Percent_Identity=35.4014598540146, Blast_Score=151, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17561440, Length=220, Percent_Identity=27.7272727272727, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921466, Length=242, Percent_Identity=30.5785123966942, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_EHRCR (Q2GGV3)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507330.1
- ProteinModelPortal:   Q2GGV3
- SMR:   Q2GGV3
- STRING:   Q2GGV3
- GeneID:   3928034
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0515
- TIGR:   ECH_0515
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   ECHA205920:ECH_0515-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 31568; Mature: 31568

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDINAVKELRNLTGAGVGDCKEALNSCGGDIEKAKNYLREQGIAKAYKKSTKDVSDGL
CCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCE
VAVHVNGNQGAILEVNSETDFVARNEKFQKLVLNLVSLANQYAVEDIEDFLKHEYVSGTS
EEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IHDEIMTNIAVIGENIHLNKIGYLSVNAGVVGGYIHSPVVNNLGKIGAIVALESTADNDK
HHHHHHHHHHHHCCCEEECEEEEEEECCCEECCHHHHHHHHHHHHHHEEEEEECCCCCHH
LNVLARQIAMHIVAARPEALSVDLLDKDILDKEREIIKKQVDQLNKPVSVAERIIDGRIA
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KFYQDVVLLEQIFVMDNQLTISELIKKKESELGASINLVGYKLFVISK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEEEC
>Mature Secondary Structure
MKVDINAVKELRNLTGAGVGDCKEALNSCGGDIEKAKNYLREQGIAKAYKKSTKDVSDGL
CCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCE
VAVHVNGNQGAILEVNSETDFVARNEKFQKLVLNLVSLANQYAVEDIEDFLKHEYVSGTS
EEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IHDEIMTNIAVIGENIHLNKIGYLSVNAGVVGGYIHSPVVNNLGKIGAIVALESTADNDK
HHHHHHHHHHHHCCCEEECEEEEEEECCCEECCHHHHHHHHHHHHHHEEEEEECCCCCHH
LNVLARQIAMHIVAARPEALSVDLLDKDILDKEREIIKKQVDQLNKPVSVAERIIDGRIA
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KFYQDVVLLEQIFVMDNQLTISELIKKKESELGASINLVGYKLFVISK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA