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The map label for this gene is rpiB [C]

Identifier: 88658472

GI number: 88658472

Start: 643735

End: 644181

Strand: Reverse

Name: rpiB [C]

Synonym: ECH_0638

Alternate gene names: 88658472

Gene position: 644181-643735 (Counterclockwise)

Preceding gene: 88658578

Following gene: 88657957

Centisome position: 54.77

GC content: 32.44

Gene sequence:

>447_bases
ATGTTGACTATGCAGAAAATTTTCATTTCTTCTGATCATGCTGGAATAGAGTTAAGATTAGTTATTAAGTTATATTTAAG
CAATTTAGGTTATGTAGTAGAGGATTATGGTTGCATGGAATCTAGTATTGCTGTAGATTACCCGGATTATGTACAAAATG
TTGTAGATTGTGTCATTAACACTTTAGGGTTCGGTATTTTAATATGTGGTACAGGTATTGGCATGAGTATTGTGGCAAAT
CGTCACAGAAAAATTAGGGCAGCTTTGTGTTATAATGTAGAAGTAGCTAAGTTAGCACGTGAGCATAATAATGCTAATGT
ACTTTGTCTTGGTGCTAGATATATTAATCATGGAGAAGCTCAGTCTATAGTTTATCAGTTCATTACTACAAAATTTTCTG
AAGGGAGGCATGTACAAAGAATAGCAAAAATAGATAATTGTTTATGA

Upstream 100 bases:

>100_bases
CTTGAAATTGTAAAGAGAAATTGTAACGTTTGGGTTGTGACTATGTTTATGATATTGACTTTTCATAGTATTTCATATAG
ATATTAGATCATGTTAAATT

Downstream 100 bases:

>100_bases
GTCAAACGGTTGATCAAAATGAGTTATTAAAGTTTTCAAAAATAGCTGATAAATGGTGGGATGAACATGGCCCATTAAAA
GCCTTGCATGATATACATCC

Product: ribose 5-phosphate isomerase B

Products: D-Ribulose 5-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MLTMQKIFISSDHAGIELRLVIKLYLSNLGYVVEDYGCMESSIAVDYPDYVQNVVDCVINTLGFGILICGTGIGMSIVAN
RHRKIRAALCYNVEVAKLAREHNNANVLCLGARYINHGEAQSIVYQFITTKFSEGRHVQRIAKIDNCL

Sequences:

>Translated_148_residues
MLTMQKIFISSDHAGIELRLVIKLYLSNLGYVVEDYGCMESSIAVDYPDYVQNVVDCVINTLGFGILICGTGIGMSIVAN
RHRKIRAALCYNVEVAKLAREHNNANVLCLGARYINHGEAQSIVYQFITTKFSEGRHVQRIAKIDNCL
>Mature_148_residues
MLTMQKIFISSDHAGIELRLVIKLYLSNLGYVVEDYGCMESSIAVDYPDYVQNVVDCVINTLGFGILICGTGIGMSIVAN
RHRKIRAALCYNVEVAKLAREHNNANVLCLGARYINHGEAQSIVYQFITTKFSEGRHVQRIAKIDNCL

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=142, Percent_Identity=40.1408450704225, Blast_Score=94, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6 [C]

Molecular weight: Translated: 16514; Mature: 16514

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMQKIFISSDHAGIELRLVIKLYLSNLGYVVEDYGCMESSIAVDYPDYVQNVVDCVIN
CCCHHEEEEECCCCCEEHHHHHHHHHHHCCCEECCCCCCCCCEEECCHHHHHHHHHHHHH
TLGFGILICGTGIGMSIVANRHRKIRAALCYNVEVAKLAREHNNANVLCLGARYINHGEA
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCHH
QSIVYQFITTKFSEGRHVQRIAKIDNCL
HHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLTMQKIFISSDHAGIELRLVIKLYLSNLGYVVEDYGCMESSIAVDYPDYVQNVVDCVIN
CCCHHEEEEECCCCCEEHHHHHHHHHHHCCCEECCCCCCCCCEEECCHHHHHHHHHHHHH
TLGFGILICGTGIGMSIVANRHRKIRAALCYNVEVAKLAREHNNANVLCLGARYINHGEA
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCHH
QSIVYQFITTKFSEGRHVQRIAKIDNCL
HHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: alpha-D-Ribose 5-phosphate [C]

Specific reaction: alpha-D-Ribose 5-phosphate <==> D-Ribulose 5-phosphate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA