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The map label for this gene is miaA

Identifier: 88658369

GI number: 88658369

Start: 595400

End: 596311

Strand: Direct

Name: miaA

Synonym: ECH_0588

Alternate gene names: 88658369

Gene position: 595400-596311 (Clockwise)

Preceding gene: 88658620

Following gene: 88658282

Centisome position: 50.62

GC content: 28.62

Gene sequence:

>912_bases
ATGAATAATATATTAATCATTACTGGACCTACAGCATCAGGAAAGTCTAAGATATCTATGAAAATAGCGCAAGATAATAA
TGGAATTATTGTGAATTGTGATTCAAAGCAAATATATCGAGAAATCCCTATAATTACAGATCAACCGAATTTGAATGATA
CTTCTGTAGAACACAAATTATATGGATATGTATCAGTAACACAACAATATTCTGTAGGATTATGGATAGAAGATTTAAAG
GATGAAATATCATCCATTATACAACAAAAAAAGTTTCCAGTTATAACTGGAGGTAGTGGAATGTATATCAATAGTCTAAT
ATATGGCTTATCGCAAATTCCTAAGATAGAAGATAGTGTAAGGAATGAAACAAGAAGGCTATTTAAAACTCTAGGGAAAA
AAGAGTTTTATGCATTATTAATAGATAAAGATCCTATAGCAAAATGTTTACATAAAAACAATTCACATCAATTATTAAGA
GCATATGAAGTCATAGAACAAACAGGAATTTCCATATTTGTATGGAAGGAAAATGCACCTCGTGAACCTATATTTAAGAA
TTTTAAGCTTTGTATTCTTATGCCACCACGAAGCGAAATATATAAAAAAATAAACGAACGTTTTATTAATATGATCAATA
CATCTGTAATAGAAGAAATAGAAAATTTGCTTTCACTGAATATACCTGCACATTTTCCTGCAATGAAAGCACACGGTGTG
CCAGAAATAATTCAATATTTACAAAATAAAATCAGTATTGATCAAGCAATAGAAATAGCTCAAAAAAATACTAGGAATTA
TGCTAAACGTCAATATACATGGTTTAAGCATCAATTCCGAAATGCATTATTCTATGAATCTCAGGATCAGTTGTTAGAAT
CTATAAAAAACAGTTATGTATATTATAACTGA

Upstream 100 bases:

>100_bases
TACCTTGATAAAATCATGTATTGTAAAATAACCAGTTTATAATACTATATGATATATTTTATACATACAATATAAGTTAA
CTTAATTATGCATAATTAAC

Downstream 100 bases:

>100_bases
ATTCAAAACTGTTCGAATTAAAACACCTTAATCCAATAATAATGTCATTTCTTGTCTTGCTCTATTTTTTTGTAGCCTTT
ACAATCTACATTTAGATTTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNNILIITGPTASGKSKISMKIAQDNNGIIVNCDSKQIYREIPIITDQPNLNDTSVEHKLYGYVSVTQQYSVGLWIEDLK
DEISSIIQQKKFPVITGGSGMYINSLIYGLSQIPKIEDSVRNETRRLFKTLGKKEFYALLIDKDPIAKCLHKNNSHQLLR
AYEVIEQTGISIFVWKENAPREPIFKNFKLCILMPPRSEIYKKINERFINMINTSVIEEIENLLSLNIPAHFPAMKAHGV
PEIIQYLQNKISIDQAIEIAQKNTRNYAKRQYTWFKHQFRNALFYESQDQLLESIKNSYVYYN

Sequences:

>Translated_303_residues
MNNILIITGPTASGKSKISMKIAQDNNGIIVNCDSKQIYREIPIITDQPNLNDTSVEHKLYGYVSVTQQYSVGLWIEDLK
DEISSIIQQKKFPVITGGSGMYINSLIYGLSQIPKIEDSVRNETRRLFKTLGKKEFYALLIDKDPIAKCLHKNNSHQLLR
AYEVIEQTGISIFVWKENAPREPIFKNFKLCILMPPRSEIYKKINERFINMINTSVIEEIENLLSLNIPAHFPAMKAHGV
PEIIQYLQNKISIDQAIEIAQKNTRNYAKRQYTWFKHQFRNALFYESQDQLLESIKNSYVYYN
>Mature_303_residues
MNNILIITGPTASGKSKISMKIAQDNNGIIVNCDSKQIYREIPIITDQPNLNDTSVEHKLYGYVSVTQQYSVGLWIEDLK
DEISSIIQQKKFPVITGGSGMYINSLIYGLSQIPKIEDSVRNETRRLFKTLGKKEFYALLIDKDPIAKCLHKNNSHQLLR
AYEVIEQTGISIFVWKENAPREPIFKNFKLCILMPPRSEIYKKINERFINMINTSVIEEIENLLSLNIPAHFPAMKAHGV
PEIIQYLQNKISIDQAIEIAQKNTRNYAKRQYTWFKHQFRNALFYESQDQLLESIKNSYVYYN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=23.3009708737864, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1790613, Length=279, Percent_Identity=25.089605734767, Blast_Score=115, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=306, Percent_Identity=25.4901960784314, Blast_Score=102, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_EHRCR (Q2GGN4)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507399.1
- ProteinModelPortal:   Q2GGN4
- STRING:   Q2GGN4
- GeneID:   3928069
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0588
- TIGR:   ECH_0588
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK749553
- BioCyc:   ECHA205920:ECH_0588-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35102; Mature: 35102

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNILIITGPTASGKSKISMKIAQDNNGIIVNCDSKQIYREIPIITDQPNLNDTSVEHKL
CCCEEEEECCCCCCCCEEEEEEEECCCCEEEEECCHHHHHHCCCEECCCCCCCCCHHHHE
YGYVSVTQQYSVGLWIEDLKDEISSIIQQKKFPVITGGSGMYINSLIYGLSQIPKIEDSV
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHH
RNETRRLFKTLGKKEFYALLIDKDPIAKCLHKNNSHQLLRAYEVIEQTGISIFVWKENAP
HHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCC
REPIFKNFKLCILMPPRSEIYKKINERFINMINTSVIEEIENLLSLNIPAHFPAMKAHGV
CCCHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCH
PEIIQYLQNKISIDQAIEIAQKNTRNYAKRQYTWFKHQFRNALFYESQDQLLESIKNSYV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCE
YYN
ECC
>Mature Secondary Structure
MNNILIITGPTASGKSKISMKIAQDNNGIIVNCDSKQIYREIPIITDQPNLNDTSVEHKL
CCCEEEEECCCCCCCCEEEEEEEECCCCEEEEECCHHHHHHCCCEECCCCCCCCCHHHHE
YGYVSVTQQYSVGLWIEDLKDEISSIIQQKKFPVITGGSGMYINSLIYGLSQIPKIEDSV
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHH
RNETRRLFKTLGKKEFYALLIDKDPIAKCLHKNNSHQLLRAYEVIEQTGISIFVWKENAP
HHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCC
REPIFKNFKLCILMPPRSEIYKKINERFINMINTSVIEEIENLLSLNIPAHFPAMKAHGV
CCCHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCH
PEIIQYLQNKISIDQAIEIAQKNTRNYAKRQYTWFKHQFRNALFYESQDQLLESIKNSYV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCE
YYN
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA