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The map label for this gene is clpA [C]

Identifier: 88658335

GI number: 88658335

Start: 573069

End: 575366

Strand: Direct

Name: clpA [C]

Synonym: ECH_0567

Alternate gene names: 88658335

Gene position: 573069-575366 (Clockwise)

Preceding gene: 88658489

Following gene: 88658159

Centisome position: 48.72

GC content: 29.59

Gene sequence:

>2298_bases
ATGAAATTGGAGATGTCAGTGTTATCAAAGAATCTAGAAGCTAGTTTACACAAGGCACTGTCAATTGCATTTGATTTTCA
TCATGAGTATGCTACTTTGGAACATCTTTTATTAGCTTTAACTGATGATATAGATGCAAGGCGTGTTTTTTATGCTTTTC
GTGTATCAATTGATAAACTGAAAATTACAATTATAAATTTTCTGAGATATGAAATACCTACTTTAATTGATAAGGGTATT
TCAGAAGTTAAGCCTACTGCTATTTTTGAACGCTTAATACATAGAGCTATTATACATGCTCATACTTCTGGTAAGAGTGA
GGTAACAGGTGCAAATATTCTTGCTGAAATTTTATCAGAAAAGGATTCTTATAGTGCATGTTTTTTACATGAACAAAATA
TAAAATACATAGATGTTGTAAATTATACTTCTAATAATAATTTATATGCTGGTGAGTTTAATGTAGATCAGGAATTTGTA
AAATATAACGAGTATGGAAAATCGATAGGTAACGTAAATAAAGATATATTGAAGGATAATGAAACTCTAGATAGCTATTG
TGTTAATTTAAATGAAGCAGCAAAAAAAGGAAAAATAGATTATGTAATAGGAAGAGCATATGAATTAGAGAGAACAATGG
AGGTTCTATTAAGGAGAAGGAAAAATAACCCATTATACGTTGGTGATCCTGGAGTTGGAAAAACTGCGATTGTTGAAGGA
TTAGCATTAAAAATTATAGAAGGTGATGTACCAGATCAGTTAAAAAAAATGGTAATATATTCTTTAGATATGGGTGCATT
ATTAGCTGGAACACGCTATCGTGGAGATTTTGAAGAGAGAATAAAATCTGTTATTAAGGCAATAGAAGCAAAGGAAAATG
CAATATTGTTTATTGATGAAATTCATACGATTGTTGGGGCTGGATCTACTAGTGGTGGATCTCTTGATGCAAGTAATTTA
TTAAAGCCTGCTTTAGCTAGAGGGACATTAAGATGTATAGGTGCTACTACTTATAAAGAATATAATAATAATTTTGAAAA
GGATAGAGCACTAGCTAGAAGATATCAAAAAATTAATGTTGAGGAGTCATCAGTTGGTGAAACATTAAGGATATTAGATG
GAATAAAATCTTATTATGAATCACATCATCAAGTTAGGTATACTAATCAAGCAATTAAATATGCTGCAGAATTATCGGAC
AGGTATATTTCAGGAAAAATGCTTCCAGATAAAGCTGTAGATGTTATAGATGAAGCAGGTGTATATTGTAAATTGCATAA
CACTGGTAATAAAGTTATTACTGGAAGTGATATTGAGCATATAATTTCTAGGATTACTGAAATACCATGTAGTAACTTGT
TGTTTAATGATTTGGATAGAGTAAAAAATTTAGAAGAAAATCTAAAAAAAGATATATTTGGTCAAGATTTTGCAATTTCT
CATCTTGTAGATTCGATTAAAATTGCCAAAGCAGGGCTTAGAAATTATAACAAACCTTTAGCAAGTTATTTATTTTCTGG
GCCAACTGGAGTAGGTAAGACTGAATTGGCGAGGCAGTTAGCAAATCATATGGGTATGAAATTAATAAGATTTGATATGT
CTGAATATATGGAATCTCATGCAATTTCTAAAATTATAGGGTCTCCTCCAGGTTATGTAGGATATGATCAAGGAGGATTA
TTAACTGATTCTATTTCAAGACATCAGTATAGTGTATTGTTACTTGATGAAATAGAAAAGGCTCACAGTGATATATATAA
TATATTACTACAGATAATGGATTATGGGTGTGTTACTGATACTTATGGTAAAAAGGTTAATTTTCATAATGTAGTAATTA
TATTAACAACTAATGCTGGTGCTTTTGAGTTAAGTAAAAGTTCTATAGGATTTATTAGAAATAAAAGTTTTACTCATGGA
GATAATGAAAAAGCAATTGAAAGGATCTTTAGTCCAGAATTTCGCAATAGGTTGGATGCTATAATTTCATTTTCATCTTT
AGATCAAGAAGTGATGTTACGTATAGTTAAAAAGTTTATTTATCAGCTTAAAGAGCAATTAGCAAAGAAAAATGTGCATA
TAGATGTAGCTGATGATGTGTTAATGTATTTAGCTCAGTCTGGTTATAATGATGCTTATGGTGTAAGAAATATAGAAAAT
ATTATTTCTAAAAAAGTTAAGAAGTATTTAGCAGAAGAGATATTATTTGGTAAATTATTTAATGGTGGAAATGTAAAAAT
CAAACTTGATGCTGATACTAATAAGTTAGTGTATGATTTTTGTTGTGCAGAAGCATAA

Upstream 100 bases:

>100_bases
ATATAATATTAAAAATGTATTGAATATTATTGATCCTGTAATGCTTATATTTTTATTTTCAACTTATTTTTAATTCTTAG
GTGGTAGAGTTTTTTAAATT

Downstream 100 bases:

>100_bases
ATTTAATATATTAAAGATATTGCTTAAATATAAAATATATATTAAAAGTAATGTATTATCTATATCAATGAACTTTATTT
TATAAGTGCTATTTTTATAA

Product: ATP-dependent Clp protease, ATP-binding subunit ClpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MKLEMSVLSKNLEASLHKALSIAFDFHHEYATLEHLLLALTDDIDARRVFYAFRVSIDKLKITIINFLRYEIPTLIDKGI
SEVKPTAIFERLIHRAIIHAHTSGKSEVTGANILAEILSEKDSYSACFLHEQNIKYIDVVNYTSNNNLYAGEFNVDQEFV
KYNEYGKSIGNVNKDILKDNETLDSYCVNLNEAAKKGKIDYVIGRAYELERTMEVLLRRRKNNPLYVGDPGVGKTAIVEG
LALKIIEGDVPDQLKKMVIYSLDMGALLAGTRYRGDFEERIKSVIKAIEAKENAILFIDEIHTIVGAGSTSGGSLDASNL
LKPALARGTLRCIGATTYKEYNNNFEKDRALARRYQKINVEESSVGETLRILDGIKSYYESHHQVRYTNQAIKYAAELSD
RYISGKMLPDKAVDVIDEAGVYCKLHNTGNKVITGSDIEHIISRITEIPCSNLLFNDLDRVKNLEENLKKDIFGQDFAIS
HLVDSIKIAKAGLRNYNKPLASYLFSGPTGVGKTELARQLANHMGMKLIRFDMSEYMESHAISKIIGSPPGYVGYDQGGL
LTDSISRHQYSVLLLDEIEKAHSDIYNILLQIMDYGCVTDTYGKKVNFHNVVIILTTNAGAFELSKSSIGFIRNKSFTHG
DNEKAIERIFSPEFRNRLDAIISFSSLDQEVMLRIVKKFIYQLKEQLAKKNVHIDVADDVLMYLAQSGYNDAYGVRNIEN
IISKKVKKYLAEEILFGKLFNGGNVKIKLDADTNKLVYDFCCAEA

Sequences:

>Translated_765_residues
MKLEMSVLSKNLEASLHKALSIAFDFHHEYATLEHLLLALTDDIDARRVFYAFRVSIDKLKITIINFLRYEIPTLIDKGI
SEVKPTAIFERLIHRAIIHAHTSGKSEVTGANILAEILSEKDSYSACFLHEQNIKYIDVVNYTSNNNLYAGEFNVDQEFV
KYNEYGKSIGNVNKDILKDNETLDSYCVNLNEAAKKGKIDYVIGRAYELERTMEVLLRRRKNNPLYVGDPGVGKTAIVEG
LALKIIEGDVPDQLKKMVIYSLDMGALLAGTRYRGDFEERIKSVIKAIEAKENAILFIDEIHTIVGAGSTSGGSLDASNL
LKPALARGTLRCIGATTYKEYNNNFEKDRALARRYQKINVEESSVGETLRILDGIKSYYESHHQVRYTNQAIKYAAELSD
RYISGKMLPDKAVDVIDEAGVYCKLHNTGNKVITGSDIEHIISRITEIPCSNLLFNDLDRVKNLEENLKKDIFGQDFAIS
HLVDSIKIAKAGLRNYNKPLASYLFSGPTGVGKTELARQLANHMGMKLIRFDMSEYMESHAISKIIGSPPGYVGYDQGGL
LTDSISRHQYSVLLLDEIEKAHSDIYNILLQIMDYGCVTDTYGKKVNFHNVVIILTTNAGAFELSKSSIGFIRNKSFTHG
DNEKAIERIFSPEFRNRLDAIISFSSLDQEVMLRIVKKFIYQLKEQLAKKNVHIDVADDVLMYLAQSGYNDAYGVRNIEN
IISKKVKKYLAEEILFGKLFNGGNVKIKLDADTNKLVYDFCCAEA
>Mature_765_residues
MKLEMSVLSKNLEASLHKALSIAFDFHHEYATLEHLLLALTDDIDARRVFYAFRVSIDKLKITIINFLRYEIPTLIDKGI
SEVKPTAIFERLIHRAIIHAHTSGKSEVTGANILAEILSEKDSYSACFLHEQNIKYIDVVNYTSNNNLYAGEFNVDQEFV
KYNEYGKSIGNVNKDILKDNETLDSYCVNLNEAAKKGKIDYVIGRAYELERTMEVLLRRRKNNPLYVGDPGVGKTAIVEG
LALKIIEGDVPDQLKKMVIYSLDMGALLAGTRYRGDFEERIKSVIKAIEAKENAILFIDEIHTIVGAGSTSGGSLDASNL
LKPALARGTLRCIGATTYKEYNNNFEKDRALARRYQKINVEESSVGETLRILDGIKSYYESHHQVRYTNQAIKYAAELSD
RYISGKMLPDKAVDVIDEAGVYCKLHNTGNKVITGSDIEHIISRITEIPCSNLLFNDLDRVKNLEENLKKDIFGQDFAIS
HLVDSIKIAKAGLRNYNKPLASYLFSGPTGVGKTELARQLANHMGMKLIRFDMSEYMESHAISKIIGSPPGYVGYDQGGL
LTDSISRHQYSVLLLDEIEKAHSDIYNILLQIMDYGCVTDTYGKKVNFHNVVIILTTNAGAFELSKSSIGFIRNKSFTHG
DNEKAIERIFSPEFRNRLDAIISFSSLDQEVMLRIVKKFIYQLKEQLAKKNVHIDVADDVLMYLAQSGYNDAYGVRNIEN
IISKKVKKYLAEEILFGKLFNGGNVKIKLDADTNKLVYDFCCAEA

Specific function: ATP-Dependent Specificity Component Of The Clpp Protease. It Directs The Protease To Specific Substrates. The Primary Function Of The Clpa-Clpp Complex Appears To Be The Degradation Of Unfolded Or Abnormal Proteins. [C]

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=329, Percent_Identity=31.306990881459, Blast_Score=153, Evalue=6e-37,
Organism=Escherichia coli, GI1787109, Length=761, Percent_Identity=47.9632063074901, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1788943, Length=247, Percent_Identity=55.8704453441296, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=250, Percent_Identity=50.4, Blast_Score=250, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6323002, Length=341, Percent_Identity=40.4692082111437, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR013461
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 86116; Mature: 86116

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEMSVLSKNLEASLHKALSIAFDFHHEYATLEHLLLALTDDIDARRVFYAFRVSIDKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEHHHH
KITIINFLRYEIPTLIDKGISEVKPTAIFERLIHRAIIHAHTSGKSEVTGANILAEILSE
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
KDSYSACFLHEQNIKYIDVVNYTSNNNLYAGEFNVDQEFVKYNEYGKSIGNVNKDILKDN
CCCCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHCCC
ETLDSYCVNLNEAAKKGKIDYVIGRAYELERTMEVLLRRRKNNPLYVGDPGVGKTAIVEG
CCHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHH
LALKIIEGDVPDQLKKMVIYSLDMGALLAGTRYRGDFEERIKSVIKAIEAKENAILFIDE
HHEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEH
IHTIVGAGSTSGGSLDASNLLKPALARGTLRCIGATTYKEYNNNFEKDRALARRYQKINV
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHHCCC
EESSVGETLRILDGIKSYYESHHQVRYTNQAIKYAAELSDRYISGKMLPDKAVDVIDEAG
CHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
VYCKLHNTGNKVITGSDIEHIISRITEIPCSNLLFNDLDRVKNLEENLKKDIFGQDFAIS
CEEEEECCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HLVDSIKIAKAGLRNYNKPLASYLFSGPTGVGKTELARQLANHMGMKLIRFDMSEYMESH
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
AISKIIGSPPGYVGYDQGGLLTDSISRHQYSVLLLDEIEKAHSDIYNILLQIMDYGCVTD
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
TYGKKVNFHNVVIILTTNAGAFELSKSSIGFIRNKSFTHGDNEKAIERIFSPEFRNRLDA
CCCCEEECCEEEEEEEECCCCEEECCCCCCHHCCCCCCCCCCHHHHHHHHCHHHHHHHHH
IISFSSLDQEVMLRIVKKFIYQLKEQLAKKNVHIDVADDVLMYLAQSGYNDAYGVRNIEN
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHHH
IISKKVKKYLAEEILFGKLFNGGNVKIKLDADTNKLVYDFCCAEA
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCEEHHHHHCCC
>Mature Secondary Structure
MKLEMSVLSKNLEASLHKALSIAFDFHHEYATLEHLLLALTDDIDARRVFYAFRVSIDKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEHHHH
KITIINFLRYEIPTLIDKGISEVKPTAIFERLIHRAIIHAHTSGKSEVTGANILAEILSE
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
KDSYSACFLHEQNIKYIDVVNYTSNNNLYAGEFNVDQEFVKYNEYGKSIGNVNKDILKDN
CCCCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHCCC
ETLDSYCVNLNEAAKKGKIDYVIGRAYELERTMEVLLRRRKNNPLYVGDPGVGKTAIVEG
CCHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHH
LALKIIEGDVPDQLKKMVIYSLDMGALLAGTRYRGDFEERIKSVIKAIEAKENAILFIDE
HHEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEH
IHTIVGAGSTSGGSLDASNLLKPALARGTLRCIGATTYKEYNNNFEKDRALARRYQKINV
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHHCCC
EESSVGETLRILDGIKSYYESHHQVRYTNQAIKYAAELSDRYISGKMLPDKAVDVIDEAG
CHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
VYCKLHNTGNKVITGSDIEHIISRITEIPCSNLLFNDLDRVKNLEENLKKDIFGQDFAIS
CEEEEECCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HLVDSIKIAKAGLRNYNKPLASYLFSGPTGVGKTELARQLANHMGMKLIRFDMSEYMESH
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
AISKIIGSPPGYVGYDQGGLLTDSISRHQYSVLLLDEIEKAHSDIYNILLQIMDYGCVTD
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
TYGKKVNFHNVVIILTTNAGAFELSKSSIGFIRNKSFTHGDNEKAIERIFSPEFRNRLDA
CCCCEEECCEEEEEEEECCCCEEECCCCCCHHCCCCCCCCCCHHHHHHHHCHHHHHHHHH
IISFSSLDQEVMLRIVKKFIYQLKEQLAKKNVHIDVADDVLMYLAQSGYNDAYGVRNIEN
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHHH
IISKKVKKYLAEEILFGKLFNGGNVKIKLDADTNKLVYDFCCAEA
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6209404 [H]