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The map label for this gene is ndk

Identifier: 88658311

GI number: 88658311

Start: 1142222

End: 1142650

Strand: Reverse

Name: ndk

Synonym: ECH_1117

Alternate gene names: 88658311

Gene position: 1142650-1142222 (Counterclockwise)

Preceding gene: 88657898

Following gene: 88658075

Centisome position: 97.14

GC content: 32.4

Gene sequence:

>429_bases
TTGTTAGAGAAAACTTTGTCTATATTAAAACCAGATGTGATAAAGCGTAATATTACTGGGCAGGTGAATTCTTATATAGA
AAATTCAGGACTTAAAATAATTATACAAAAGATGTGTTTACTAACACGTTGTCAAGCTGAAGAATTTTATGCGATACATA
AATCTCAACATTTTTTTGTACCTCTAATTGATTTTATGGTATCTGGTCCAATAATAGTGCAAGTGTTGCAAGGAGAAAAT
GCTATAAGCTTATATAGAGAAATTATGGGTGCTACAGATCCTAAAAAAGCAAGTCCTGGAACGATTAGAGCAGATTTTGC
TGAGAATATAGATGCAAATTGTGTCCATGGTTCGGATAGTTTAGATAATGCCATGAGAGAAATAAGATTTTTCTTTAGTG
ACTATGAGCTCCTAGCGTTAAATGGGTAA

Upstream 100 bases:

>100_bases
ATGAAATATAAGATAAAATTTATTTACTTTTTAGCTTGATTGGATCAATAATTTATATTAAACATGAAAGTCAGTTTGTC
CGGGTAAGGGGGGATGTATT

Downstream 100 bases:

>100_bases
TTACCTGTATTGAAATATATTCATGTTAAAGCCTGAATTTATAATAACATGTTGTTATTTTAAGTTCAGGTACCTTGGTT
TGTTGTAATATAAGTATTTA

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MLEKTLSILKPDVIKRNITGQVNSYIENSGLKIIIQKMCLLTRCQAEEFYAIHKSQHFFVPLIDFMVSGPIIVQVLQGEN
AISLYREIMGATDPKKASPGTIRADFAENIDANCVHGSDSLDNAMREIRFFFSDYELLALNG

Sequences:

>Translated_142_residues
MLEKTLSILKPDVIKRNITGQVNSYIENSGLKIIIQKMCLLTRCQAEEFYAIHKSQHFFVPLIDFMVSGPIIVQVLQGEN
AISLYREIMGATDPKKASPGTIRADFAENIDANCVHGSDSLDNAMREIRFFFSDYELLALNG
>Mature_142_residues
MLEKTLSILKPDVIKRNITGQVNSYIENSGLKIIIQKMCLLTRCQAEEFYAIHKSQHFFVPLIDFMVSGPIIVQVLQGEN
AISLYREIMGATDPKKASPGTIRADFAENIDANCVHGSDSLDNAMREIRFFFSDYELLALNG

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family

Homologues:

Organism=Homo sapiens, GI37693993, Length=136, Percent_Identity=36.7647058823529, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI4826862, Length=138, Percent_Identity=36.231884057971, Blast_Score=107, Evalue=5e-24,
Organism=Homo sapiens, GI66392203, Length=137, Percent_Identity=33.5766423357664, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI66392227, Length=137, Percent_Identity=33.5766423357664, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI66392205, Length=137, Percent_Identity=33.5766423357664, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI4505409, Length=137, Percent_Identity=33.5766423357664, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI66392192, Length=137, Percent_Identity=33.5766423357664, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI4557797, Length=136, Percent_Identity=33.8235294117647, Blast_Score=100, Evalue=7e-22,
Organism=Homo sapiens, GI38045913, Length=136, Percent_Identity=33.8235294117647, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI7019465, Length=138, Percent_Identity=35.5072463768116, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI37574614, Length=138, Percent_Identity=35.5072463768116, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI5031951, Length=138, Percent_Identity=34.0579710144928, Blast_Score=84, Evalue=3e-17,
Organism=Homo sapiens, GI4505413, Length=136, Percent_Identity=32.3529411764706, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI30023828, Length=135, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI148839372, Length=143, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1788866, Length=136, Percent_Identity=46.3235294117647, Blast_Score=142, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17506807, Length=135, Percent_Identity=36.2962962962963, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322783, Length=136, Percent_Identity=38.9705882352941, Blast_Score=112, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45549037, Length=139, Percent_Identity=33.8129496402878, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI18860097, Length=138, Percent_Identity=34.0579710144928, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDK_EHRCR (Q2GF82)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507901.1
- ProteinModelPortal:   Q2GF82
- SMR:   Q2GF82
- STRING:   Q2GF82
- GeneID:   3928009
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_1117
- TIGR:   ECH_1117
- eggNOG:   COG0105
- HOGENOM:   HBG445152
- OMA:   ERPFFGA
- PhylomeDB:   Q2GF82
- ProtClustDB:   PRK00668
- BioCyc:   ECHA205920:ECH_1117-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00451
- InterPro:   IPR001564
- InterPro:   IPR023005
- Gene3D:   G3DSA:3.30.70.141
- PANTHER:   PTHR11349
- PRINTS:   PR01243
- SMART:   SM00562

Pfam domain/function: PF00334 NDK; SSF54919 NDK

EC number: =2.7.4.6

Molecular weight: Translated: 15957; Mature: 15957

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00469 NDP_KINASES

Important sites: ACT_SITE 116-116 BINDING 10-10 BINDING 58-58 BINDING 86-86 BINDING 92-92 BINDING 103-103 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKTLSILKPDVIKRNITGQVNSYIENSGLKIIIQKMCLLTRCQAEEFYAIHKSQHFFV
CCCHHHHHHCHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
PLIDFMVSGPIIVQVLQGENAISLYREIMGATDPKKASPGTIRADFAENIDANCVHGSDS
HHHHHHHCCHHEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEECHHCCCCCCEECCCCH
LDNAMREIRFFFSDYELLALNG
HHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MLEKTLSILKPDVIKRNITGQVNSYIENSGLKIIIQKMCLLTRCQAEEFYAIHKSQHFFV
CCCHHHHHHCHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
PLIDFMVSGPIIVQVLQGENAISLYREIMGATDPKKASPGTIRADFAENIDANCVHGSDS
HHHHHHHCCHHEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEECHHCCCCCCEECCCCH
LDNAMREIRFFFSDYELLALNG
HHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA