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The map label for this gene is rpoA [H]

Identifier: 88658298

GI number: 88658298

Start: 409729

End: 410754

Strand: Direct

Name: rpoA [H]

Synonym: ECH_0432

Alternate gene names: 88658298

Gene position: 409729-410754 (Clockwise)

Preceding gene: 88657813

Following gene: 88657838

Centisome position: 34.83

GC content: 31.77

Gene sequence:

>1026_bases
ATGAGTGATCATTGGAGTAAATTAACTAAGCCTTCTTCTATTAAGGTAGTTCCTAGTGGTGCTTCTCCAAATAAGGCAGA
TTTAATTATTGAACCTCTTGAGAGTGGTTTTGCTTTAACTCTAGGTAATGCTTTAAGAAGAGTTATGATGTCTTCTTTGC
GTGGTTTTGCTGTTTATGGTGTTGAAATAGAGAATGTTTTACACGAATTTACTTCGATTTCTGGGGTTAGAGAAGATGTA
ACAGATATTCTTTTAAATATTAGCATGATGAGAGTAAAGCTTTCTGGTATGAATAGTAAAGTTTTGTCTCTTAAAGTTAA
AGGACCATGTGAAGTAAGATCTGGAATGATACCTGACACTGATGATTGCATTATACTCAATAAGGATTTGTTAATTTGCA
CTTTAGATCAAGGTGTAGATTTTAATATAAAAATGTATGTAAATGCTGGTAAAGGTTATGTTCCTGCTGTAAAGCGTAAG
TCAGTTAATAAACTTGGTGATATACCAATAAATTTCATTGCAACTAATGCTCTTTATAGCCCTATAAAAAAGGCGTCTTT
TAAAGTAGAAAGTAGTCGTATTGGTCAATTTACAGATTATGATCGATTGATTATGTCTGTGGAAACAGATGGGTCTATTT
TACCTGATGAAGCAGTAGCATTAGCTGCAAGAATTTTACAAGATCAATTTCAACCATTTATTAATTTTGATGAAACTGAT
GAGCCTCATAAGAAAGTTGATGCTAAAGATACATTGCCTTATGATTCTAATCTGTTACGTAAAGTTGATGAGTTAGAACT
TTCAGTAAGGTCTTATAATTGTTTGAAAAATGATAATATTACTTATATAGGAGATTTAGTCCAAAAGACTGAATCTGATA
TGTTAAGAACTCCAAATTTTGGTAGAAAGTCGCTTAATGAGATTAATGAGCTTTTAGCAAGTATGAATTTGCATTTAGGA
ATGAAAATTGCGAATTGGCCTCCTGAATCCATTGAGAGTTTAAGTAAACAATATAGTGAAGAATGA

Upstream 100 bases:

>100_bases
TTCTATTGTGATTTTCGATGAGGATTCTAATTCTATTGCACAAGATTCGGGTTATATGGCAGTAGGAAATGCATATTCTA
ATGGAGTGAGTTATATATCA

Downstream 100 bases:

>100_bases
GGTTATATTATGAGGCATCGTGTTGCTCATAGAAAGTTTTCTCGTACCAGTGCTCATAGAATGTCTATGCTTTTAAATTT
GTGTATATCTTTAATTAAGC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSDHWSKLTKPSSIKVVPSGASPNKADLIIEPLESGFALTLGNALRRVMMSSLRGFAVYGVEIENVLHEFTSISGVREDV
TDILLNISMMRVKLSGMNSKVLSLKVKGPCEVRSGMIPDTDDCIILNKDLLICTLDQGVDFNIKMYVNAGKGYVPAVKRK
SVNKLGDIPINFIATNALYSPIKKASFKVESSRIGQFTDYDRLIMSVETDGSILPDEAVALAARILQDQFQPFINFDETD
EPHKKVDAKDTLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLVQKTESDMLRTPNFGRKSLNEINELLASMNLHLG
MKIANWPPESIESLSKQYSEE

Sequences:

>Translated_341_residues
MSDHWSKLTKPSSIKVVPSGASPNKADLIIEPLESGFALTLGNALRRVMMSSLRGFAVYGVEIENVLHEFTSISGVREDV
TDILLNISMMRVKLSGMNSKVLSLKVKGPCEVRSGMIPDTDDCIILNKDLLICTLDQGVDFNIKMYVNAGKGYVPAVKRK
SVNKLGDIPINFIATNALYSPIKKASFKVESSRIGQFTDYDRLIMSVETDGSILPDEAVALAARILQDQFQPFINFDETD
EPHKKVDAKDTLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLVQKTESDMLRTPNFGRKSLNEINELLASMNLHLG
MKIANWPPESIESLSKQYSEE
>Mature_340_residues
SDHWSKLTKPSSIKVVPSGASPNKADLIIEPLESGFALTLGNALRRVMMSSLRGFAVYGVEIENVLHEFTSISGVREDVT
DILLNISMMRVKLSGMNSKVLSLKVKGPCEVRSGMIPDTDDCIILNKDLLICTLDQGVDFNIKMYVNAGKGYVPAVKRKS
VNKLGDIPINFIATNALYSPIKKASFKVESSRIGQFTDYDRLIMSVETDGSILPDEAVALAARILQDQFQPFINFDETDE
PHKKVDAKDTLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLVQKTESDMLRTPNFGRKSLNEINELLASMNLHLGM
KIANWPPESIESLSKQYSEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=332, Percent_Identity=41.566265060241, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 38001; Mature: 37870

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHWSKLTKPSSIKVVPSGASPNKADLIIEPLESGFALTLGNALRRVMMSSLRGFAVYG
CCCHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCEEEEE
VEIENVLHEFTSISGVREDVTDILLNISMMRVKLSGMNSKVLSLKVKGPCEVRSGMIPDT
EEHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCHHHHCCCCCCC
DDCIILNKDLLICTLDQGVDFNIKMYVNAGKGYVPAVKRKSVNKLGDIPINFIATNALYS
CCEEEEECCEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHEEEHHHHHH
PIKKASFKVESSRIGQFTDYDRLIMSVETDGSILPDEAVALAARILQDQFQPFINFDETD
HHHHHHEEECHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
EPHKKVDAKDTLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLVQKTESDMLRTPNF
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCC
GRKSLNEINELLASMNLHLGMKIANWPPESIESLSKQYSEE
CHHHHHHHHHHHHHCCHHCCEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDHWSKLTKPSSIKVVPSGASPNKADLIIEPLESGFALTLGNALRRVMMSSLRGFAVYG
CCHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCEEEEE
VEIENVLHEFTSISGVREDVTDILLNISMMRVKLSGMNSKVLSLKVKGPCEVRSGMIPDT
EEHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCHHHHCCCCCCC
DDCIILNKDLLICTLDQGVDFNIKMYVNAGKGYVPAVKRKSVNKLGDIPINFIATNALYS
CCEEEEECCEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHEEEHHHHHH
PIKKASFKVESSRIGQFTDYDRLIMSVETDGSILPDEAVALAARILQDQFQPFINFDETD
HHHHHHEEECHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
EPHKKVDAKDTLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLVQKTESDMLRTPNF
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCC
GRKSLNEINELLASMNLHLGMKIANWPPESIESLSKQYSEE
CHHHHHHHHHHHHHCCHHCCEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA