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The map label for this gene is pgk [H]

Identifier: 88658248

GI number: 88658248

Start: 47910

End: 49061

Strand: Direct

Name: pgk [H]

Synonym: ECH_0055

Alternate gene names: 88658248

Gene position: 47910-49061 (Clockwise)

Preceding gene: 88658071

Following gene: 88657848

Centisome position: 4.07

GC content: 31.16

Gene sequence:

>1152_bases
ATGAAAAAAATACAAGATTTTAGTTGTAGTGGTAAAACTGTATTGCTGCGTGCGGATTTAAATGTTCCAGTAGATAATGG
GATAGTTTTGGATGATACTAGGATTGTTAGATTAACTACAACTATAAAATATTTATTGAGTAATGATGCGAAAATTGTCA
TAATGTCGCACTATGGATCTCCTAAATCTTATGATAAAGAATTTTCTTTAAAGTTTGTGGTTGAGTATTTGAATAAAATA
TTTGCAACAAATGTAGTATTTATAGATGGTGTAATTGGAGATTATGTAGAACAGACTATTCAATCTGTTCCAGCAGGGAC
TATATTGCTGCTAGAAAATTTGAGATTTTACGCAGAAGAAGAAAAGAATGATTTGAATTTTGCAAAACAACTTGCGTTGT
TGGCTGATATATATGTTAATGATGCTTTTTCTTGTTTACATCGTAAGCATGCTTCTATAGATGCGATTACTAGAGTTATG
CCGTCTTTTATTGGCTTTAATTTTCAAGAAGAAATGAAATATTTGAGTTGTGTTGTTTCAAATAGTGAGAAGCCAGTAGC
TGTTATAGTTGGTGGTTCAAAAATATCAACAAAAGTTCATATGTTAAAAAATTTGATTAAAAAAATAGATTTTTTGATAG
TGGGAGGAGCCATTGCGAATAATTTTTTGTTATCACAAGGTTTAAAAATAGGTAAGTCGTTATACGAAGAGTTAGAAAAA
GATCTTGTAACAGAAATTGTAGATCTTGCTAAGAGATATGAATGTAAGATAATTGTCCCTGTTGATTACGTAGTGGCTAA
AAATTACATTTGTGGGGATAGTACAATAAAAGACAATGACACTTTAGAGTCTGATGATATGATATTAGATGTAGGACCTC
AAACTGTTAACATGATTGCTGCTACGATAAATAAATGTAGAACAGTGCTATGGAATGGTCCGTGTGGTATGTTTGAAAAA
GAACCTTTTTCTAAAGGAACATTTAGTGTTGCGAACTTGTTGTCAAAATTGACTAAGGTAGGAAAGCTAAAAAGTATTGT
TGGAGGTGGAGATAGTATATGTGCAATAAAATTATCTGGACTTTCAAATGAAGACTTTACTTATATTTCTACAGGAGGAG
GAGCTTTATTGCATTTTTTGAGTATCGCATGA

Upstream 100 bases:

>100_bases
TTCTGTAGTTTATAAAGATATTTAACTATTTTAGTGATTTATTAAATTGTTAAACAAGGTAACTTATATGTTAAAGCCTC
ATGATTTTACTTTTCGTAAT

Downstream 100 bases:

>100_bases
TTTTAAAAGTAATATAAAGTTCACTTTAAAGTGTATGTATTGTCTAAATATGACAGAGTTTTATTGGTAATCAGTTTAAA
ATGTGTTTTCTTTAATGTAG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKKIQDFSCSGKTVLLRADLNVPVDNGIVLDDTRIVRLTTTIKYLLSNDAKIVIMSHYGSPKSYDKEFSLKFVVEYLNKI
FATNVVFIDGVIGDYVEQTIQSVPAGTILLLENLRFYAEEEKNDLNFAKQLALLADIYVNDAFSCLHRKHASIDAITRVM
PSFIGFNFQEEMKYLSCVVSNSEKPVAVIVGGSKISTKVHMLKNLIKKIDFLIVGGAIANNFLLSQGLKIGKSLYEELEK
DLVTEIVDLAKRYECKIIVPVDYVVAKNYICGDSTIKDNDTLESDDMILDVGPQTVNMIAATINKCRTVLWNGPCGMFEK
EPFSKGTFSVANLLSKLTKVGKLKSIVGGGDSICAIKLSGLSNEDFTYISTGGGALLHFLSIA

Sequences:

>Translated_383_residues
MKKIQDFSCSGKTVLLRADLNVPVDNGIVLDDTRIVRLTTTIKYLLSNDAKIVIMSHYGSPKSYDKEFSLKFVVEYLNKI
FATNVVFIDGVIGDYVEQTIQSVPAGTILLLENLRFYAEEEKNDLNFAKQLALLADIYVNDAFSCLHRKHASIDAITRVM
PSFIGFNFQEEMKYLSCVVSNSEKPVAVIVGGSKISTKVHMLKNLIKKIDFLIVGGAIANNFLLSQGLKIGKSLYEELEK
DLVTEIVDLAKRYECKIIVPVDYVVAKNYICGDSTIKDNDTLESDDMILDVGPQTVNMIAATINKCRTVLWNGPCGMFEK
EPFSKGTFSVANLLSKLTKVGKLKSIVGGGDSICAIKLSGLSNEDFTYISTGGGALLHFLSIA
>Mature_383_residues
MKKIQDFSCSGKTVLLRADLNVPVDNGIVLDDTRIVRLTTTIKYLLSNDAKIVIMSHYGSPKSYDKEFSLKFVVEYLNKI
FATNVVFIDGVIGDYVEQTIQSVPAGTILLLENLRFYAEEEKNDLNFAKQLALLADIYVNDAFSCLHRKHASIDAITRVM
PSFIGFNFQEEMKYLSCVVSNSEKPVAVIVGGSKISTKVHMLKNLIKKIDFLIVGGAIANNFLLSQGLKIGKSLYEELEK
DLVTEIVDLAKRYECKIIVPVDYVVAKNYICGDSTIKDNDTLESDDMILDVGPQTVNMIAATINKCRTVLWNGPCGMFEK
EPFSKGTFSVANLLSKLTKVGKLKSIVGGGDSICAIKLSGLSNEDFTYISTGGGALLHFLSIA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=395, Percent_Identity=32.9113924050633, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI31543397, Length=395, Percent_Identity=32.6582278481013, Blast_Score=215, Evalue=7e-56,
Organism=Escherichia coli, GI1789294, Length=384, Percent_Identity=39.5833333333333, Blast_Score=247, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17508823, Length=398, Percent_Identity=36.4321608040201, Blast_Score=261, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6319857, Length=399, Percent_Identity=32.5814536340852, Blast_Score=233, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24581203, Length=399, Percent_Identity=36.0902255639098, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17136394, Length=402, Percent_Identity=34.5771144278607, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42206; Mature: 42206

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQDFSCSGKTVLLRADLNVPVDNGIVLDDTRIVRLTTTIKYLLSNDAKIVIMSHYGS
CCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCEEEEHHHHHHHHHCCCCEEEEEEECCC
PKSYDKEFSLKFVVEYLNKIFATNVVFIDGVIGDYVEQTIQSVPAGTILLLENLRFYAEE
CCCCCCHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHCCCCEEEEEHHHHHHHHC
EKNDLNFAKQLALLADIYVNDAFSCLHRKHASIDAITRVMPSFIGFNFQEEMKYLSCVVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
NSEKPVAVIVGGSKISTKVHMLKNLIKKIDFLIVGGAIANNFLLSQGLKIGKSLYEELEK
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
DLVTEIVDLAKRYECKIIVPVDYVVAKNYICGDSTIKDNDTLESDDMILDVGPQTVNMIA
HHHHHHHHHHHHCCCEEEEECHHHHHCCEECCCCCCCCCCCCCCCCEEEECCCHHHHHHH
ATINKCRTVLWNGPCGMFEKEPFSKGTFSVANLLSKLTKVGKLKSIVGGGDSICAIKLSG
HHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
LSNEDFTYISTGGGALLHFLSIA
CCCCCEEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MKKIQDFSCSGKTVLLRADLNVPVDNGIVLDDTRIVRLTTTIKYLLSNDAKIVIMSHYGS
CCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCEEEEHHHHHHHHHCCCCEEEEEEECCC
PKSYDKEFSLKFVVEYLNKIFATNVVFIDGVIGDYVEQTIQSVPAGTILLLENLRFYAEE
CCCCCCHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHCCCCEEEEEHHHHHHHHC
EKNDLNFAKQLALLADIYVNDAFSCLHRKHASIDAITRVMPSFIGFNFQEEMKYLSCVVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
NSEKPVAVIVGGSKISTKVHMLKNLIKKIDFLIVGGAIANNFLLSQGLKIGKSLYEELEK
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
DLVTEIVDLAKRYECKIIVPVDYVVAKNYICGDSTIKDNDTLESDDMILDVGPQTVNMIA
HHHHHHHHHHHHCCCEEEEECHHHHHCCEECCCCCCCCCCCCCCCCEEEECCCHHHHHHH
ATINKCRTVLWNGPCGMFEKEPFSKGTFSVANLLSKLTKVGKLKSIVGGGDSICAIKLSG
HHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
LSNEDFTYISTGGGALLHFLSIA
CCCCCEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA