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The map label for this gene is gltX

Identifier: 88658242

GI number: 88658242

Start: 197517

End: 198848

Strand: Direct

Name: gltX

Synonym: ECH_0208

Alternate gene names: 88658242

Gene position: 197517-198848 (Clockwise)

Preceding gene: 161486752

Following gene: 88658238

Centisome position: 16.79

GC content: 33.03

Gene sequence:

>1332_bases
ATGATTACACGTTTTGCTCCGAGTCCGACAGGATATCTTCATGTTGGAAATGTTAGAACGGCTTTAGTTTGTTGGTTATA
TGCTCGTAAGCAAAATGGTAAGTTTTTGTTAAGGTTTGATGATACTGATACTCAGAGGTCGAAAGACGAGTATATAAGGG
AAATAGAGAATGATTTAGTGTGGTTAAATATAAATTGGGACTCTAGTTTTAGACAGTCTTCTAGATTTGACCGCTATGAG
GATGTGTTTAATTATCTGTTGAAGGAAGGGTTGATATATCCTTGCTATGAAAGCAAAGAAGAACTCGAATTTAAAAGGAA
AATGAAATTAAAGTTAGGTTTACCGCCTATTTACGATAGAAGTGCATTGAATCTAACTCAAGCGGAAAAAGATAAGTATT
CTGAAAGAGCTCCATATTTTAGATTTAAAATAGATCAGAATCAGTTAATAAGCTGGAATGATGAGGTACGTGGTAAAGTA
TCATTTAACTCAGAGAATATTAGCGATCCAATCATTAGGAGGGTTGATGGAACATATACTTATATGCTGCCATCTATAAT
AGATGACATGGATTTTAATGTTACTCACATAATACGAGGGGAAGATCATATAAGTAATACAGCAGTTCAAATACAAATGT
TACATGCTTTGAAAGCTAGTATACCTATATTTTCGCATTTGTCTTTACTGTATTCTGATGATAATAAAATATCTAAAAGA
GTTGGTGGTTCTAGTGTGAAAGATATGCAATCTTATGGGTTAGAACCTATGGCTATTAATAGTTATTTTGCTAAAATAGG
TACTTCTAATCCAGTTAGTGTACACACGAGAATGTGTGGGTTAATAGATTCTTTTGATATTACTACTTTTAGTCAAGCTC
CAACTAAATTTAATATAGATGATGTATTAAAATTAAATCCCAAGGTATTATGTAGTATGTCTTTTGATGATGTAAGAAAT
AGGTTACAGTCATTTAATATAACTTCTCCAAGTTTTTGGAATTTTGTATGTGGGAATATAGACAAATTCTCTGATATTGA
AGGATGGGCAAAAATTTGTAGTGGGGATATGATACCTGTGATTGGGCAAGATGATAAAGATTTTATCATGTTAGCACTAA
ATATGCTCCCTCAGGGAGAAGTACGTGATAATACTTGGAATCTGTGGATTTCTAATATAAAGCAGCATACTGATCGTAGA
GCTAAAAATTTATTTACTCCTTTACGGTTAGCGTTGACTGGATTATCTACAGGTCCAGAGCTTGCAAAGTTATTGCCTTT
GATTGGTAGGGTGGAAATTGTACGTAGACTTAGTTATTCTGAAGTACAATAA

Upstream 100 bases:

>100_bases
AGCATTTAAAAATAGAGTATTAGTGATTTGATTGTGTCAATTAATTTATCTTGTTCATATTATTTATTTGTGTAGTGTTG
AATTTTAGGAGATCTTGGTT

Downstream 100 bases:

>100_bases
ATTATTTTATTACTGAGTGTCTTAATATAATGTATTTCTATGTTGTATTTGATCATTTATTGTGATAAAAGTAAGAGATA
TTTCTTTAATTCGATGGCGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MITRFAPSPTGYLHVGNVRTALVCWLYARKQNGKFLLRFDDTDTQRSKDEYIREIENDLVWLNINWDSSFRQSSRFDRYE
DVFNYLLKEGLIYPCYESKEELEFKRKMKLKLGLPPIYDRSALNLTQAEKDKYSERAPYFRFKIDQNQLISWNDEVRGKV
SFNSENISDPIIRRVDGTYTYMLPSIIDDMDFNVTHIIRGEDHISNTAVQIQMLHALKASIPIFSHLSLLYSDDNKISKR
VGGSSVKDMQSYGLEPMAINSYFAKIGTSNPVSVHTRMCGLIDSFDITTFSQAPTKFNIDDVLKLNPKVLCSMSFDDVRN
RLQSFNITSPSFWNFVCGNIDKFSDIEGWAKICSGDMIPVIGQDDKDFIMLALNMLPQGEVRDNTWNLWISNIKQHTDRR
AKNLFTPLRLALTGLSTGPELAKLLPLIGRVEIVRRLSYSEVQ

Sequences:

>Translated_443_residues
MITRFAPSPTGYLHVGNVRTALVCWLYARKQNGKFLLRFDDTDTQRSKDEYIREIENDLVWLNINWDSSFRQSSRFDRYE
DVFNYLLKEGLIYPCYESKEELEFKRKMKLKLGLPPIYDRSALNLTQAEKDKYSERAPYFRFKIDQNQLISWNDEVRGKV
SFNSENISDPIIRRVDGTYTYMLPSIIDDMDFNVTHIIRGEDHISNTAVQIQMLHALKASIPIFSHLSLLYSDDNKISKR
VGGSSVKDMQSYGLEPMAINSYFAKIGTSNPVSVHTRMCGLIDSFDITTFSQAPTKFNIDDVLKLNPKVLCSMSFDDVRN
RLQSFNITSPSFWNFVCGNIDKFSDIEGWAKICSGDMIPVIGQDDKDFIMLALNMLPQGEVRDNTWNLWISNIKQHTDRR
AKNLFTPLRLALTGLSTGPELAKLLPLIGRVEIVRRLSYSEVQ
>Mature_443_residues
MITRFAPSPTGYLHVGNVRTALVCWLYARKQNGKFLLRFDDTDTQRSKDEYIREIENDLVWLNINWDSSFRQSSRFDRYE
DVFNYLLKEGLIYPCYESKEELEFKRKMKLKLGLPPIYDRSALNLTQAEKDKYSERAPYFRFKIDQNQLISWNDEVRGKV
SFNSENISDPIIRRVDGTYTYMLPSIIDDMDFNVTHIIRGEDHISNTAVQIQMLHALKASIPIFSHLSLLYSDDNKISKR
VGGSSVKDMQSYGLEPMAINSYFAKIGTSNPVSVHTRMCGLIDSFDITTFSQAPTKFNIDDVLKLNPKVLCSMSFDDVRN
RLQSFNITSPSFWNFVCGNIDKFSDIEGWAKICSGDMIPVIGQDDKDFIMLALNMLPQGEVRDNTWNLWISNIKQHTDRR
AKNLFTPLRLALTGLSTGPELAKLLPLIGRVEIVRRLSYSEVQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=26.6260162601626, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4826960, Length=284, Percent_Identity=24.6478873239437, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI62241042, Length=256, Percent_Identity=24.609375, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788743, Length=473, Percent_Identity=32.9809725158562, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=30.1652892561983, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786895, Length=167, Percent_Identity=32.3353293413174, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=37.0517928286853, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17543332, Length=294, Percent_Identity=23.469387755102, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=321, Percent_Identity=35.5140186915888, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321192, Length=235, Percent_Identity=26.8085106382979, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=36.1111111111111, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665321, Length=478, Percent_Identity=28.4518828451883, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24649466, Length=245, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864488, Length=331, Percent_Identity=23.2628398791541, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_EHRCR (Q2GHQ1)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507032.1
- STRING:   Q2GHQ1
- GeneID:   3927352
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0208
- TIGR:   ECH_0208
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   RSKQEYI
- ProtClustDB:   PRK12558
- BioCyc:   ECHA205920:ECH_0208-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51095; Mature: 51095

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRFAPSPTGYLHVGNVRTALVCWLYARKQNGKFLLRFDDTDTQRSKDEYIREIENDLV
CCCCCCCCCCCEEEECCHHHHHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHHCCCEE
WLNINWDSSFRQSSRFDRYEDVFNYLLKEGLIYPCYESKEELEFKRKMKLKLGLPPIYDR
EEEECCCCHHHHHHCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHEEECCCCCCCC
SALNLTQAEKDKYSERAPYFRFKIDQNQLISWNDEVRGKVSFNSENISDPIIRRVDGTYT
CCCCCHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCEEEEECCCCCCCHHHHHCCCCEE
YMLPSIIDDMDFNVTHIIRGEDHISNTAVQIQMLHALKASIPIFSHLSLLYSDDNKISKR
EECHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHEEEECCCCHHHHH
VGGSSVKDMQSYGLEPMAINSYFAKIGTSNPVSVHTRMCGLIDSFDITTFSQAPTKFNID
CCCCCHHHHHHCCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
DVLKLNPKVLCSMSFDDVRNRLQSFNITSPSFWNFVCGNIDKFSDIEGWAKICSGDMIPV
HHHCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEE
IGQDDKDFIMLALNMLPQGEVRDNTWNLWISNIKQHTDRRAKNLFTPLRLALTGLSTGPE
ECCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LAKLLPLIGRVEIVRRLSYSEVQ
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITRFAPSPTGYLHVGNVRTALVCWLYARKQNGKFLLRFDDTDTQRSKDEYIREIENDLV
CCCCCCCCCCCEEEECCHHHHHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHHCCCEE
WLNINWDSSFRQSSRFDRYEDVFNYLLKEGLIYPCYESKEELEFKRKMKLKLGLPPIYDR
EEEECCCCHHHHHHCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHEEECCCCCCCC
SALNLTQAEKDKYSERAPYFRFKIDQNQLISWNDEVRGKVSFNSENISDPIIRRVDGTYT
CCCCCHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCEEEEECCCCCCCHHHHHCCCCEE
YMLPSIIDDMDFNVTHIIRGEDHISNTAVQIQMLHALKASIPIFSHLSLLYSDDNKISKR
EECHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHEEEECCCCHHHHH
VGGSSVKDMQSYGLEPMAINSYFAKIGTSNPVSVHTRMCGLIDSFDITTFSQAPTKFNID
CCCCCHHHHHHCCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
DVLKLNPKVLCSMSFDDVRNRLQSFNITSPSFWNFVCGNIDKFSDIEGWAKICSGDMIPV
HHHCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEE
IGQDDKDFIMLALNMLPQGEVRDNTWNLWISNIKQHTDRRAKNLFTPLRLALTGLSTGPE
ECCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LAKLLPLIGRVEIVRRLSYSEVQ
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA