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The map label for this gene is folC [H]

Identifier: 88658221

GI number: 88658221

Start: 710800

End: 712107

Strand: Reverse

Name: folC [H]

Synonym: ECH_0702

Alternate gene names: 88658221

Gene position: 712107-710800 (Counterclockwise)

Preceding gene: 88658517

Following gene: 88657743

Centisome position: 60.54

GC content: 34.17

Gene sequence:

>1308_bases
ATGGTAAGTATGCCCCACTGGCCTAAACCTATAGGTTTAGCACCGGCTGATCTGGTTTGTGATATGATTCCTGGTAGAAT
GAGACAATTATTAGATAGGCTAGGTAATCCTGAAAAAAAATTACCTCCAGTGATACATATTGCTGGAACTAATGGTAAGG
GGTCAACTATATCGTTTATACGTTATATTTTGAATTCTGCAGGTCTAAAAACTCATATTTACACTTCGCCACACTTAGTA
GAATTTAATGAAAGAGTAGTCCTTGCTGGAAAAAGAATATCTGATCAATATTTACATGAGGTTATAGAGATTTGTAGAGA
AGCCAGTGAGGGTATCCCTGTTACTTTTTTTGAAGGTACAACTGCAGCAATATTATTAGCATTTTCAAAAGTTGACGCAG
ATATTGTATTAGTAGAAACAGGTGTTGGTGGACGTCTTGATGCAACAAATGTAGTACACCCAATATTAAATATTATTACT
TCAATATCATTAGAGCATACAGAAGCATTAGGCAATACTGTTGAATTAATAGCTGGTGAGAAAGCTGGAATAATGAAAGA
GAATGTTACTTGTGTTATTGCCCCACAAGATCAAGAATCAATAATGAACGTTCTAGAATATCATGCAATTAAGAAAAACG
TTCCGTTATACCGAGGGGGAATTGATTGGTTTTGTGAAAGGAAAGGCGATAGTATGCTTTTTAAGAGTAGTGTTGATGAG
TTACTTTTTCCATTGCCCTCATTACGTGGAGAACATCAGATAATAAATGCAGGTAATGCCATAGCTGCTTGTAGTATACT
TTCTGGTAAGTTTGGGTATAATATAGATTATGAAAATATTGTTCAAGGTTTAAAAGAAGCTTATTGGCCAGCTAGATTAG
AAAAAATTACTACTGGTTTCCTATCTAAAATGGTGCCTCAACTTTGGGATTTGTTTTTAGATGGGGCTCATAATCCTGCC
GGAGGTGAAATGCTATCACGTTGGATACAGAATAATGAATTAGGAGATTTGTACATTATTATTGGAATGACTAGAGAAAA
AGATAGTGCTGTATTTTTAAATTATTTAAAACCTTATATAAAATTCTTGTCTAGTGTTTGTATAAAATCTGAACCAAGAG
CTCAAACTGCAGCAGAACTATTACAGACTGCATTGTCATTAGGTATAAAAGCTGTTGCGGCTAAGACTTTGGCAGATGCA
ATTTCTAAAATATTAGAAGTAGGAGATTGTAATAGAAAGTCTACAATTTTAATTTGTGGGTCATTATATTTAGCCGGAGA
TGTTTTATTAGAAAACAGTATGGAGTAA

Upstream 100 bases:

>100_bases
TTTTTAAAAGTTTGGGTATTGATATTTATTGGATAGGGTATATAATATCTTGTATCTAGTTATGCAATCATTTTTTTTGA
GTTACCTAAGGAGGTAGGGT

Downstream 100 bases:

>100_bases
TATATTTCTGCAGAGTTATTTAATTTGATACATGCAGTTTTATTATTATCTTGAAGTTTTTATAGAGAAAGTCGAGTTAA
GGTAACACGGTATCCTTCTG

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MVSMPHWPKPIGLAPADLVCDMIPGRMRQLLDRLGNPEKKLPPVIHIAGTNGKGSTISFIRYILNSAGLKTHIYTSPHLV
EFNERVVLAGKRISDQYLHEVIEICREASEGIPVTFFEGTTAAILLAFSKVDADIVLVETGVGGRLDATNVVHPILNIIT
SISLEHTEALGNTVELIAGEKAGIMKENVTCVIAPQDQESIMNVLEYHAIKKNVPLYRGGIDWFCERKGDSMLFKSSVDE
LLFPLPSLRGEHQIINAGNAIAACSILSGKFGYNIDYENIVQGLKEAYWPARLEKITTGFLSKMVPQLWDLFLDGAHNPA
GGEMLSRWIQNNELGDLYIIIGMTREKDSAVFLNYLKPYIKFLSSVCIKSEPRAQTAAELLQTALSLGIKAVAAKTLADA
ISKILEVGDCNRKSTILICGSLYLAGDVLLENSME

Sequences:

>Translated_435_residues
MVSMPHWPKPIGLAPADLVCDMIPGRMRQLLDRLGNPEKKLPPVIHIAGTNGKGSTISFIRYILNSAGLKTHIYTSPHLV
EFNERVVLAGKRISDQYLHEVIEICREASEGIPVTFFEGTTAAILLAFSKVDADIVLVETGVGGRLDATNVVHPILNIIT
SISLEHTEALGNTVELIAGEKAGIMKENVTCVIAPQDQESIMNVLEYHAIKKNVPLYRGGIDWFCERKGDSMLFKSSVDE
LLFPLPSLRGEHQIINAGNAIAACSILSGKFGYNIDYENIVQGLKEAYWPARLEKITTGFLSKMVPQLWDLFLDGAHNPA
GGEMLSRWIQNNELGDLYIIIGMTREKDSAVFLNYLKPYIKFLSSVCIKSEPRAQTAAELLQTALSLGIKAVAAKTLADA
ISKILEVGDCNRKSTILICGSLYLAGDVLLENSME
>Mature_435_residues
MVSMPHWPKPIGLAPADLVCDMIPGRMRQLLDRLGNPEKKLPPVIHIAGTNGKGSTISFIRYILNSAGLKTHIYTSPHLV
EFNERVVLAGKRISDQYLHEVIEICREASEGIPVTFFEGTTAAILLAFSKVDADIVLVETGVGGRLDATNVVHPILNIIT
SISLEHTEALGNTVELIAGEKAGIMKENVTCVIAPQDQESIMNVLEYHAIKKNVPLYRGGIDWFCERKGDSMLFKSSVDE
LLFPLPSLRGEHQIINAGNAIAACSILSGKFGYNIDYENIVQGLKEAYWPARLEKITTGFLSKMVPQLWDLFLDGAHNPA
GGEMLSRWIQNNELGDLYIIIGMTREKDSAVFLNYLKPYIKFLSSVCIKSEPRAQTAAELLQTALSLGIKAVAAKTLADA
ISKILEVGDCNRKSTILICGSLYLAGDVLLENSME

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=367, Percent_Identity=29.1553133514986, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI66932984, Length=367, Percent_Identity=29.1553133514986, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1788654, Length=394, Percent_Identity=30.9644670050761, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=340, Percent_Identity=30, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=341, Percent_Identity=30.2052785923754, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=341, Percent_Identity=30.2052785923754, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=430, Percent_Identity=30.2325581395349, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324815, Length=359, Percent_Identity=25.3481894150418, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322718, Length=298, Percent_Identity=25.1677852348993, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641571, Length=448, Percent_Identity=27.0089285714286, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24581568, Length=147, Percent_Identity=34.0136054421769, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47705; Mature: 47705

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSMPHWPKPIGLAPADLVCDMIPGRMRQLLDRLGNPEKKLPPVIHIAGTNGKGSTISFI
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHCCCEEEEECCCCCCHHHHHH
RYILNSAGLKTHIYTSPHLVEFNERVVLAGKRISDQYLHEVIEICREASEGIPVTFFEGT
HHHHHCCCCEEEEECCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
TAAILLAFSKVDADIVLVETGVGGRLDATNVVHPILNIITSISLEHTEALGNTVELIAGE
HHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECC
KAGIMKENVTCVIAPQDQESIMNVLEYHAIKKNVPLYRGGIDWFCERKGDSMLFKSSVDE
CCCCEECCCEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCCCHHHHHHHHH
LLFPLPSLRGEHQIINAGNAIAACSILSGKFGYNIDYENIVQGLKEAYWPARLEKITTGF
HHHCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
LSKMVPQLWDLFLDGAHNPAGGEMLSRWIQNNELGDLYIIIGMTREKDSAVFLNYLKPYI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
KFLSSVCIKSEPRAQTAAELLQTALSLGIKAVAAKTLADAISKILEVGDCNRKSTILICG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
SLYLAGDVLLENSME
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSMPHWPKPIGLAPADLVCDMIPGRMRQLLDRLGNPEKKLPPVIHIAGTNGKGSTISFI
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHCCCEEEEECCCCCCHHHHHH
RYILNSAGLKTHIYTSPHLVEFNERVVLAGKRISDQYLHEVIEICREASEGIPVTFFEGT
HHHHHCCCCEEEEECCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
TAAILLAFSKVDADIVLVETGVGGRLDATNVVHPILNIITSISLEHTEALGNTVELIAGE
HHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECC
KAGIMKENVTCVIAPQDQESIMNVLEYHAIKKNVPLYRGGIDWFCERKGDSMLFKSSVDE
CCCCEECCCEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCCCHHHHHHHHH
LLFPLPSLRGEHQIINAGNAIAACSILSGKFGYNIDYENIVQGLKEAYWPARLEKITTGF
HHHCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
LSKMVPQLWDLFLDGAHNPAGGEMLSRWIQNNELGDLYIIIGMTREKDSAVFLNYLKPYI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
KFLSSVCIKSEPRAQTAAELLQTALSLGIKAVAAKTLADAISKILEVGDCNRKSTILICG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
SLYLAGDVLLENSME
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]