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The map label for this gene is leuS

Identifier: 88658156

GI number: 88658156

Start: 809295

End: 811784

Strand: Reverse

Name: leuS

Synonym: ECH_0794

Alternate gene names: 88658156

Gene position: 811784-809295 (Counterclockwise)

Preceding gene: 88658451

Following gene: 88658121

Centisome position: 69.01

GC content: 32.13

Gene sequence:

>2490_bases
ATGCATTATGATTTCAAAAAAGTAGAACAAGATATACAAGGAAAATGGAATTTTTATACTGATGCAAAACAAGCTCAGTG
CTACGTTTTAGAGATGTTTCCTTATCCTTCTGGTAACATACATATGGGTCACTTGAGGAATTATACTATAGGTGATGTTA
TTGCGAGATATAAACGTGCTTGTGGTATTAATGTCTTTCATCCGATAGGATGGGATGCTTTTGGATTACCAGCAGAAAAT
GCTGCTTTATCTTATAATATAAATCCACATACTTGGACTGAAAGCAATATTGATAATATGAGATGTCAGTTGAAATCTAT
AGGATTATCCTATGATTGGGATAAGGAATTAGCTACTTGTGATCCAAGTTATTATAAACATGAACAAGCATTTTTTCTTG
ATTTTCTAAAGTGTGGTTTAGCTTATCGCAAAGAGTCATTGGTAAATTGGGATCCTGTGGATCAAACTGTGCTTGCTAAT
GAACAAGTTATAGATGGAAGGGGATGGCGTTCTGGAGCTGTGGTAGAAAAAAGAAAATTATTTCAATGGTTTCTAAAGAT
TACTGATTTTGCAGAAGAGTTATTGAATGACTTACAAATCTTAGATCAGTGGCCAGAGAAAGTAAAGTTGATGCAAGAAA
AATGGATTGGTAAGTCTCAGGGTGTAGTAATAGATTTTGAAATTTTAGGTATTAATAAAACGTTACAAGTTTTTACTACA
TGTCCCCATACATTGTTTGGAGCAGCATTTATTGCAGTATCTTTTGATCATCCGATTTTACAATATGTTAATGATTCAAA
AGTAATTCAATTGATAAATGATTTTGACAGAAAAAATTTAATCAGTGATGTATCTAGTACAATAGAAAAATTTGGAATAG
ATTCTGGATTAGTTGTAAAACATCCTTTGTTAAATGTAAATTTACCAGTTTATATTGTAAATTTTGTCTTAATGGATTAT
GCAACGGGGGCAATTTTTGGTTGTCCAGCACATGATCAGCGTGATTTTGAATTTGCAAAGAAATATTCCTTGCCAGTAAA
GCAAGTGGTATTTCCTGAAGTTGATGTGAACTTAGATAAAGAAGCTTATGTTGGCTCAGGTATTATGAAACACTCTGATT
TTTTAGATGGTATGACTGTAGATGAAGCTAAAAAAGCTATAGTTGCAAAGCTTACATTTTTAGGGGTGTGCAAAGAAATA
ACATATTATAGAATGCATGATTGGGGGATATCTAGACAACGTTATTGGGGGTGTCCTATACCGATAATATATTGTAAAAA
ATGTGGTACGGTTCCTGTAGATAAAAAAGATTTACCAGTAACTTTACCTAAAGATGTTGATTTTACAAAATATGGTAATC
CTTTAGACAATCATCCTACTTGGAAATATGTAAAATGTCCATCTTGTGGTATGGATGCTGAACGTGAGACTGATACTTTT
GACACTTTTTTTGAATCTTCTTGGTATTTTGCAGCATTTTGTGGAACTAGCAATGGAATAAATAAGGATGTGTGTAACAT
GTTATTACCAGTCAATTATTATGTAGGTGGGGTAGAACATGCAGTATTGCATTTGTTGTATTCTAGATTCTTCTGTCGAG
CATTAACTAAGTGTGGATATTTTAATATTAAGGAACCTTTTTCTAATTTGATAACGCAAGGTATGGTTTGTCATTCTACT
TATTCGGATGAGCAGGGTAATTACTTATTTCCTGAAGAAGCAAAAAAAATGATGGAAAATGGTCAGCATGTTACTGTTGG
TAGAGCTGAGAAGATGAGTAAGTCGAAAAAAAATGTGGTGAATCTTGAATATATTATTGATAAGTACGGAGCAGATACAG
CAAGATTATTTATATTGTCAGATACTCCTCCTGAGCGTGATATAGAATGGCTAGATGATGGTATAGAGGGGGCAAGTAGG
TATCTTAGTAAATTATGGAGGGTAATAATTTCTTATGATAAGTTTAATTTAAATTTTAACAAAGAAAATATAATAGGAGA
TGATGTTAAATATAGAAGATCTGTGCATAAAATTTTAAGTGGCATTACAAATGATTTAGATTTTTGCCGTTTAAATTGTG
CTGTAGCTAAGTTTAGGGAATTAAGCAATATCATTAGTGAAATGATAAGAACTTCAGTAAATTGTTATGTAGTGAGTGAA
GCAATATATATTCTAATTAGAGTAATAGAACCTTTTATTCCACATATAGCTGAAAAATTATGGGAAAATATAGGGGGTAA
AGGTATGTTATGGAATCAGGTATGGCCTAAAGCAGATTCAGAGCTACTTGTTGAAAGAAATGTTACAATTGTGGTGCAGG
TTAATGGTAAATTTGTGAAAGCATTAACAGTTGCTAATGATATTGATGATGATCAACTTAAATCTATGGCTTTGGAAATA
GCTAAGAATAGAATAGGTGGCAATGTTGTAAAAGATATATATGTTATTCCAAAACGAGTAATAAATATCGTTGCTGTCAA
ACCGAGCTAA

Upstream 100 bases:

>100_bases
TGGTATGTGTATATTTTGTATTGCTTGAATTATCTGATAATGTACTGTACAACAACTAAGCTGAAATAGTTTTTAATGGA
ATTTTTATCAACGGTGTACC

Downstream 100 bases:

>100_bases
AAATTTGATAATCTGATGTATAAGTTTTTGTAGATAGCTTTTTCATTTCAAGATTATGAGTAGTAACTAATCTTTTTGGT
GGGTTTGTTTAGTATAGTAT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MHYDFKKVEQDIQGKWNFYTDAKQAQCYVLEMFPYPSGNIHMGHLRNYTIGDVIARYKRACGINVFHPIGWDAFGLPAEN
AALSYNINPHTWTESNIDNMRCQLKSIGLSYDWDKELATCDPSYYKHEQAFFLDFLKCGLAYRKESLVNWDPVDQTVLAN
EQVIDGRGWRSGAVVEKRKLFQWFLKITDFAEELLNDLQILDQWPEKVKLMQEKWIGKSQGVVIDFEILGINKTLQVFTT
CPHTLFGAAFIAVSFDHPILQYVNDSKVIQLINDFDRKNLISDVSSTIEKFGIDSGLVVKHPLLNVNLPVYIVNFVLMDY
ATGAIFGCPAHDQRDFEFAKKYSLPVKQVVFPEVDVNLDKEAYVGSGIMKHSDFLDGMTVDEAKKAIVAKLTFLGVCKEI
TYYRMHDWGISRQRYWGCPIPIIYCKKCGTVPVDKKDLPVTLPKDVDFTKYGNPLDNHPTWKYVKCPSCGMDAERETDTF
DTFFESSWYFAAFCGTSNGINKDVCNMLLPVNYYVGGVEHAVLHLLYSRFFCRALTKCGYFNIKEPFSNLITQGMVCHST
YSDEQGNYLFPEEAKKMMENGQHVTVGRAEKMSKSKKNVVNLEYIIDKYGADTARLFILSDTPPERDIEWLDDGIEGASR
YLSKLWRVIISYDKFNLNFNKENIIGDDVKYRRSVHKILSGITNDLDFCRLNCAVAKFRELSNIISEMIRTSVNCYVVSE
AIYILIRVIEPFIPHIAEKLWENIGGKGMLWNQVWPKADSELLVERNVTIVVQVNGKFVKALTVANDIDDDQLKSMALEI
AKNRIGGNVVKDIYVIPKRVINIVAVKPS

Sequences:

>Translated_829_residues
MHYDFKKVEQDIQGKWNFYTDAKQAQCYVLEMFPYPSGNIHMGHLRNYTIGDVIARYKRACGINVFHPIGWDAFGLPAEN
AALSYNINPHTWTESNIDNMRCQLKSIGLSYDWDKELATCDPSYYKHEQAFFLDFLKCGLAYRKESLVNWDPVDQTVLAN
EQVIDGRGWRSGAVVEKRKLFQWFLKITDFAEELLNDLQILDQWPEKVKLMQEKWIGKSQGVVIDFEILGINKTLQVFTT
CPHTLFGAAFIAVSFDHPILQYVNDSKVIQLINDFDRKNLISDVSSTIEKFGIDSGLVVKHPLLNVNLPVYIVNFVLMDY
ATGAIFGCPAHDQRDFEFAKKYSLPVKQVVFPEVDVNLDKEAYVGSGIMKHSDFLDGMTVDEAKKAIVAKLTFLGVCKEI
TYYRMHDWGISRQRYWGCPIPIIYCKKCGTVPVDKKDLPVTLPKDVDFTKYGNPLDNHPTWKYVKCPSCGMDAERETDTF
DTFFESSWYFAAFCGTSNGINKDVCNMLLPVNYYVGGVEHAVLHLLYSRFFCRALTKCGYFNIKEPFSNLITQGMVCHST
YSDEQGNYLFPEEAKKMMENGQHVTVGRAEKMSKSKKNVVNLEYIIDKYGADTARLFILSDTPPERDIEWLDDGIEGASR
YLSKLWRVIISYDKFNLNFNKENIIGDDVKYRRSVHKILSGITNDLDFCRLNCAVAKFRELSNIISEMIRTSVNCYVVSE
AIYILIRVIEPFIPHIAEKLWENIGGKGMLWNQVWPKADSELLVERNVTIVVQVNGKFVKALTVANDIDDDQLKSMALEI
AKNRIGGNVVKDIYVIPKRVINIVAVKPS
>Mature_829_residues
MHYDFKKVEQDIQGKWNFYTDAKQAQCYVLEMFPYPSGNIHMGHLRNYTIGDVIARYKRACGINVFHPIGWDAFGLPAEN
AALSYNINPHTWTESNIDNMRCQLKSIGLSYDWDKELATCDPSYYKHEQAFFLDFLKCGLAYRKESLVNWDPVDQTVLAN
EQVIDGRGWRSGAVVEKRKLFQWFLKITDFAEELLNDLQILDQWPEKVKLMQEKWIGKSQGVVIDFEILGINKTLQVFTT
CPHTLFGAAFIAVSFDHPILQYVNDSKVIQLINDFDRKNLISDVSSTIEKFGIDSGLVVKHPLLNVNLPVYIVNFVLMDY
ATGAIFGCPAHDQRDFEFAKKYSLPVKQVVFPEVDVNLDKEAYVGSGIMKHSDFLDGMTVDEAKKAIVAKLTFLGVCKEI
TYYRMHDWGISRQRYWGCPIPIIYCKKCGTVPVDKKDLPVTLPKDVDFTKYGNPLDNHPTWKYVKCPSCGMDAERETDTF
DTFFESSWYFAAFCGTSNGINKDVCNMLLPVNYYVGGVEHAVLHLLYSRFFCRALTKCGYFNIKEPFSNLITQGMVCHST
YSDEQGNYLFPEEAKKMMENGQHVTVGRAEKMSKSKKNVVNLEYIIDKYGADTARLFILSDTPPERDIEWLDDGIEGASR
YLSKLWRVIISYDKFNLNFNKENIIGDDVKYRRSVHKILSGITNDLDFCRLNCAVAKFRELSNIISEMIRTSVNCYVVSE
AIYILIRVIEPFIPHIAEKLWENIGGKGMLWNQVWPKADSELLVERNVTIVVQVNGKFVKALTVANDIDDDQLKSMALEI
AKNRIGGNVVKDIYVIPKRVINIVAVKPS

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=846, Percent_Identity=34.7517730496454, Blast_Score=488, Evalue=1e-138,
Organism=Escherichia coli, GI1786861, Length=858, Percent_Identity=40.9090909090909, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=388, Percent_Identity=23.4536082474227, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71997510, Length=196, Percent_Identity=48.9795918367347, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71997517, Length=196, Percent_Identity=48.9795918367347, Blast_Score=207, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI212645227, Length=307, Percent_Identity=28.6644951140065, Blast_Score=125, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323414, Length=792, Percent_Identity=40.1515151515151, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI21355409, Length=843, Percent_Identity=34.0450771055753, Blast_Score=436, Evalue=1e-122,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_EHRCR (Q2GG41)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507592.1
- ProteinModelPortal:   Q2GG41
- SMR:   Q2GG41
- STRING:   Q2GG41
- GeneID:   3927180
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0794
- TIGR:   ECH_0794
- eggNOG:   COG0495
- HOGENOM:   HBG693845
- OMA:   YARYCSP
- PhylomeDB:   Q2GG41
- ProtClustDB:   PRK00390
- BioCyc:   ECHA205920:ECH_0794-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 94869; Mature: 94869

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00073 ACYL_COA_DH_2

Important sites: BINDING 594-594

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYDFKKVEQDIQGKWNFYTDAKQAQCYVLEMFPYPSGNIHMGHLRNYTIGDVIARYKRA
CCCCHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHH
CGINVFHPIGWDAFGLPAENAALSYNINPHTWTESNIDNMRCQLKSIGLSYDWDKELATC
CCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHCC
DPSYYKHEQAFFLDFLKCGLAYRKESLVNWDPVDQTVLANEQVIDGRGWRSGAVVEKRKL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHHHH
FQWFLKITDFAEELLNDLQILDQWPEKVKLMQEKWIGKSQGVVIDFEILGINKTLQVFTT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHC
CPHTLFGAAFIAVSFDHPILQYVNDSKVIQLINDFDRKNLISDVSSTIEKFGIDSGLVVK
CCHHHHHHHHEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
HPLLNVNLPVYIVNFVLMDYATGAIFGCPAHDQRDFEFAKKYSLPVKQVVFPEVDVNLDK
CCCEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCC
EAYVGSGIMKHSDFLDGMTVDEAKKAIVAKLTFLGVCKEITYYRMHDWGISRQRYWGCPI
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
PIIYCKKCGTVPVDKKDLPVTLPKDVDFTKYGNPLDNHPTWKYVKCPSCGMDAERETDTF
CEEEECCCCCCCCCCCCCCEECCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHH
DTFFESSWYFAAFCGTSNGINKDVCNMLLPVNYYVGGVEHAVLHLLYSRFFCRALTKCGY
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
FNIKEPFSNLITQGMVCHSTYSDEQGNYLFPEEAKKMMENGQHVTVGRAEKMSKSKKNVV
CCCHHHHHHHHHCCEEEECCCCCCCCCEECHHHHHHHHHCCCEEEECCHHHHHHHHCCEE
NLEYIIDKYGADTARLFILSDTPPERDIEWLDDGIEGASRYLSKLWRVIISYDKFNLNFN
EHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
KENIIGDDVKYRRSVHKILSGITNDLDFCRLNCAVAKFRELSNIISEMIRTSVNCYVVSE
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
AIYILIRVIEPFIPHIAEKLWENIGGKGMLWNQVWPKADSELLVERNVTIVVQVNGKFVK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCEEEEEECCCEEE
ALTVANDIDDDQLKSMALEIAKNRIGGNVVKDIYVIPKRVINIVAVKPS
EEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MHYDFKKVEQDIQGKWNFYTDAKQAQCYVLEMFPYPSGNIHMGHLRNYTIGDVIARYKRA
CCCCHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHH
CGINVFHPIGWDAFGLPAENAALSYNINPHTWTESNIDNMRCQLKSIGLSYDWDKELATC
CCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHCC
DPSYYKHEQAFFLDFLKCGLAYRKESLVNWDPVDQTVLANEQVIDGRGWRSGAVVEKRKL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHHHH
FQWFLKITDFAEELLNDLQILDQWPEKVKLMQEKWIGKSQGVVIDFEILGINKTLQVFTT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHC
CPHTLFGAAFIAVSFDHPILQYVNDSKVIQLINDFDRKNLISDVSSTIEKFGIDSGLVVK
CCHHHHHHHHEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
HPLLNVNLPVYIVNFVLMDYATGAIFGCPAHDQRDFEFAKKYSLPVKQVVFPEVDVNLDK
CCCEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCC
EAYVGSGIMKHSDFLDGMTVDEAKKAIVAKLTFLGVCKEITYYRMHDWGISRQRYWGCPI
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
PIIYCKKCGTVPVDKKDLPVTLPKDVDFTKYGNPLDNHPTWKYVKCPSCGMDAERETDTF
CEEEECCCCCCCCCCCCCCEECCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHH
DTFFESSWYFAAFCGTSNGINKDVCNMLLPVNYYVGGVEHAVLHLLYSRFFCRALTKCGY
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
FNIKEPFSNLITQGMVCHSTYSDEQGNYLFPEEAKKMMENGQHVTVGRAEKMSKSKKNVV
CCCHHHHHHHHHCCEEEECCCCCCCCCEECHHHHHHHHHCCCEEEECCHHHHHHHHCCEE
NLEYIIDKYGADTARLFILSDTPPERDIEWLDDGIEGASRYLSKLWRVIISYDKFNLNFN
EHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
KENIIGDDVKYRRSVHKILSGITNDLDFCRLNCAVAKFRELSNIISEMIRTSVNCYVVSE
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
AIYILIRVIEPFIPHIAEKLWENIGGKGMLWNQVWPKADSELLVERNVTIVVQVNGKFVK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCEEEEEECCCEEE
ALTVANDIDDDQLKSMALEIAKNRIGGNVVKDIYVIPKRVINIVAVKPS
EEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA