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The map label for this gene is nuoM [H]

Identifier: 88658135

GI number: 88658135

Start: 555900

End: 557351

Strand: Direct

Name: nuoM [H]

Synonym: ECH_0555

Alternate gene names: 88658135

Gene position: 555900-557351 (Clockwise)

Preceding gene: 88657655

Following gene: 88657624

Centisome position: 47.26

GC content: 27.69

Gene sequence:

>1452_bases
ATGCTATTTTTGATGGTTTTATTACCAATATTGGGGTCATGTTTATTAGCAGTTAGTAATTTTAAGGCAAGTTCGTTATC
TATAAGAGTAATTTCTTTATTATGTGCTGGAACTTCATTTTTTATAAATATTATTGTAGCTGTAAAGTTTGATTATTTAT
ATAAAGGTTTTCAGTTTATACATAATCTGGCACCAGGTTTTGTAGTCGGTATAGATGGATTATCTTTGCCACTTCTATTA
TTAACAACTTTTTTATTTTTATTATCTGTTGTTTTTGCTTTATATAACATGCAATCAAATAATTTGCGCATATTTCTTGC
TTTACTGCTGTTATTAGAAGGATTGACCGTAGGAGTGTTTGTTTCACTTGATATAGTAATGTTCTATATATTTTTTGAAT
CTGTTTTAATTCCAATGTTTTTCATTATAGGAATGTGGGGACATGAGGATAGGATATATGCTACGTTTAAGTTGTTTTTA
TATACTTTAAGTGGTTCTTTATTATTTTTAATAGCAATATTATATATATATTTCTTTTCTGGTAAAATAAGCGATATAGG
ACAGTTGACTTATATTTTATCAAATTATTTGAACCTTAAAGCACAGAAGTGGGTATGGATTGCATTTTTTATTTCGTTTG
CGATAAAGATACCTGTTATACCTTTTCACACTTGGTTACCTGATGCTCATGTTCAAGCGCCTACAGTAGGATCTGTTTTA
TTGGCTGGTATTCTTATTAAAGTTGGTACATATGCATTACTACGGTTTTCTCTACCTATGCTACCAGAAGCTAGTATGTA
TTTTTCAAATTTTGTAATGATATTAAGCATCATAGGTGTAATTTATGCTTCATTAATATCTTTTGTTCAAACTAATATTA
AGAAATTAATAGCATATTCTTCAATAGCTCATATGGGTTTTGTGACAGCAGGGATATTTTCTTTTAATGAATATGGAATA
TCAGGAGCTGTATTTCAAATGATTAGCCATGGGTTAGTGTCCGCAGCATTATTTATTTGTGTTGGTATACTTTATAATCG
TACTCATACTTTAGAGATAGTAAGATATAGTGGATTAGCAAAAACAATGCCTAAATTTTCCATTATGTTTATATTTTTTT
CGATGGCTTCGATTGCTTTACCTGGGACTTCAGGTTTTATAGGAGAATTTTTATCTATATTAGGAATTTTTCATTATTCT
AAATTATTTTCAATCTTTATTACTATTGGAGTTATATTAGGTGCTTTATACATGCTGTTTTTATGTAAAAGAATAATCTG
GAGTGTACAAAATTGTGATTTAATTAATTGTAATCTTAATAAAAATGAGTTATTTATTTTAGTGATTTTAGCTGCTTTTA
TATTATTATTTGGATTATATCCTTATTATATCTTATTAAAATGCTTAACTCCATTCATAGAGCAGTTGTCTTTAAGAAAC
TTTGTACTTTAG

Upstream 100 bases:

>100_bases
TACAAACTGGTTTTATTTTTGATTATGCATTTATTATGCTATTGGGTTTGATAAGTATTATCATGTGGTTAATTTATAAT
AATATAAGATTTTAAAGCAT

Downstream 100 bases:

>100_bases
TAGCATTTGAGATTATTTATTATGTATTGGAATAATTTTGTTTATATAATACCAGAAATTTTTTTAGTTAGTTCATCTTT
AATCTTCTTATTATTGGGGA

Product: NADH dehydrogenase I, M subunit

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLFLMVLLPILGSCLLAVSNFKASSLSIRVISLLCAGTSFFINIIVAVKFDYLYKGFQFIHNLAPGFVVGIDGLSLPLLL
LTTFLFLLSVVFALYNMQSNNLRIFLALLLLLEGLTVGVFVSLDIVMFYIFFESVLIPMFFIIGMWGHEDRIYATFKLFL
YTLSGSLLFLIAILYIYFFSGKISDIGQLTYILSNYLNLKAQKWVWIAFFISFAIKIPVIPFHTWLPDAHVQAPTVGSVL
LAGILIKVGTYALLRFSLPMLPEASMYFSNFVMILSIIGVIYASLISFVQTNIKKLIAYSSIAHMGFVTAGIFSFNEYGI
SGAVFQMISHGLVSAALFICVGILYNRTHTLEIVRYSGLAKTMPKFSIMFIFFSMASIALPGTSGFIGEFLSILGIFHYS
KLFSIFITIGVILGALYMLFLCKRIIWSVQNCDLINCNLNKNELFILVILAAFILLFGLYPYYILLKCLTPFIEQLSLRN
FVL

Sequences:

>Translated_483_residues
MLFLMVLLPILGSCLLAVSNFKASSLSIRVISLLCAGTSFFINIIVAVKFDYLYKGFQFIHNLAPGFVVGIDGLSLPLLL
LTTFLFLLSVVFALYNMQSNNLRIFLALLLLLEGLTVGVFVSLDIVMFYIFFESVLIPMFFIIGMWGHEDRIYATFKLFL
YTLSGSLLFLIAILYIYFFSGKISDIGQLTYILSNYLNLKAQKWVWIAFFISFAIKIPVIPFHTWLPDAHVQAPTVGSVL
LAGILIKVGTYALLRFSLPMLPEASMYFSNFVMILSIIGVIYASLISFVQTNIKKLIAYSSIAHMGFVTAGIFSFNEYGI
SGAVFQMISHGLVSAALFICVGILYNRTHTLEIVRYSGLAKTMPKFSIMFIFFSMASIALPGTSGFIGEFLSILGIFHYS
KLFSIFITIGVILGALYMLFLCKRIIWSVQNCDLINCNLNKNELFILVILAAFILLFGLYPYYILLKCLTPFIEQLSLRN
FVL
>Mature_483_residues
MLFLMVLLPILGSCLLAVSNFKASSLSIRVISLLCAGTSFFINIIVAVKFDYLYKGFQFIHNLAPGFVVGIDGLSLPLLL
LTTFLFLLSVVFALYNMQSNNLRIFLALLLLLEGLTVGVFVSLDIVMFYIFFESVLIPMFFIIGMWGHEDRIYATFKLFL
YTLSGSLLFLIAILYIYFFSGKISDIGQLTYILSNYLNLKAQKWVWIAFFISFAIKIPVIPFHTWLPDAHVQAPTVGSVL
LAGILIKVGTYALLRFSLPMLPEASMYFSNFVMILSIIGVIYASLISFVQTNIKKLIAYSSIAHMGFVTAGIFSFNEYGI
SGAVFQMISHGLVSAALFICVGILYNRTHTLEIVRYSGLAKTMPKFSIMFIFFSMASIALPGTSGFIGEFLSILGIFHYS
KLFSIFITIGVILGALYMLFLCKRIIWSVQNCDLINCNLNKNELFILVILAAFILLFGLYPYYILLKCLTPFIEQLSLRN
FVL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=326, Percent_Identity=35.2760736196319, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI251831117, Length=304, Percent_Identity=26.9736842105263, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788613, Length=405, Percent_Identity=39.0123456790123, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1788827, Length=296, Percent_Identity=25.6756756756757, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788831, Length=282, Percent_Identity=25.177304964539, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788614, Length=406, Percent_Identity=23.8916256157635, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI2367154, Length=239, Percent_Identity=25.9414225941423, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788829, Length=339, Percent_Identity=23.598820058997, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693160, Length=277, Percent_Identity=24.5487364620939, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54214; Mature: 54214

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]