Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 88658087

GI number: 88658087

Start: 610385

End: 611794

Strand: Direct

Name: gltX

Synonym: ECH_0605

Alternate gene names: 88658087

Gene position: 610385-611794 (Clockwise)

Preceding gene: 88657666

Following gene: 88658329

Centisome position: 51.89

GC content: 27.73

Gene sequence:

>1410_bases
ATGTCACATAATGTAATAACTCGATTTGCCCCTTCACCAACTGGACATTTACATTTAGGAGGAGCAAGAACAGCTCTATT
CAACTGGCTATATGCTAAACATAACAATGGAAAATTTTTATTACGCATTGAAGATACTGATAAAAAAAGATCATCTAAAG
AACTTATCGACTCTATTATTAATGCAATGTCTTGGTTAAAAATTCCTTATGATGGGGAAATAGTATTACAATCAAAAAAT
ATATCTAGACATATTGAAATTGCAAATCAACTAATATTAAACAATAAAGCATATTACTGTTATTGTTCTGAGGAAGAAAT
AAATAAAGAAAAAGAAGAATTTAGTAAAAAGGGTTTATATTATAAACACAATTGTATATGGAAAAACAAAAATTTTACAA
TTGATAATCTTACTAGAGTCATTAGATTAAGAAGCCCTACTGAAGGTGTCACATCATTTGACGATAAAGTATATGGTAAT
ATCACAGTAAGTAATACACAATTAGATGATATGGTTTTATTGCGTTCTGATAACACTCCTACATATCTCCTATCCGTAGT
TGTAGATGACCATGACATGAATATTACTCATATTATTAGAGGAACAGACCATTTAACAAATACAGCACGTCAATTATTAA
TATATAATGCATTAGAATGGAATCCTCCTAAATTTGCTCATATTCCATTGATACATGATGAAGATGGTAATAAATTATCT
AAAAGACATCAAGCAATTGGCATACATGAATATAAAAATTTAGGTATATTACCAGAAGCAATATCCAATTACTTGTTAAG
AATGGGATGGAGTCATGAAGATGATGAGATTATTAGTATGGATCAAGCAATAAAGTGGTTTTCTATAAAGAATATCGGAC
AATCTCCGGCAAGATTAGATAATAAAAAACTGGAATTTTTAAACAATCACTATATCAGTCTTACTGAAGATGAAGTGATT
CTGAATATGATTATTCCAATTATAGAAAAAAAGATAGGATATATGCTAAATGAAGTAAAAAAAGGATATTTATTAAAAGG
ATTATATGAACTAAAGAAAAGGACAAAAAATCTAGTAAATTTAGCAAACGAATCTTTGTTTTACGTTGAAGATGTACCAA
TCTCTATTGATCAAGAAGCTAGTGCTATTATAAAAGATTATAAGCACGTGTTTTCTATATTATATAATAATTTATCAAGA
ATTTCAGAAAAAGAATGGAATAACAGCATACTAACATCAACAATAAAAAACATTTCACAAAACTTAGATATAAAGATTAG
TAACATCTATCATTGTCTGCGTGCATCTATTGTTGGAAGAATGAATGCTCCAAGTATTATTGAAATTATGATTAATCTTC
AACAAGAAGAATGTCTAAAACGTATAAAGTATGCACAAAATATAGAATAA

Upstream 100 bases:

>100_bases
ACTTTACTGTTGCTGGTATTATAATTCGATACATCATTATATAATTACTTTACAAAAATGTTGAAATATAAAACAATTCT
AGTTTTTATAAGATTTTTTT

Downstream 100 bases:

>100_bases
GCTTCCTCATTATAATCTTGTACAAAATGCTACTTTAAATTATTGTCTAGAATAACTTATTTATAAAAATAATACATAAC
TTACATAAAATACAATTTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSHNVITRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDKKRSSKELIDSIINAMSWLKIPYDGEIVLQSKN
ISRHIEIANQLILNNKAYYCYCSEEEINKEKEEFSKKGLYYKHNCIWKNKNFTIDNLTRVIRLRSPTEGVTSFDDKVYGN
ITVSNTQLDDMVLLRSDNTPTYLLSVVVDDHDMNITHIIRGTDHLTNTARQLLIYNALEWNPPKFAHIPLIHDEDGNKLS
KRHQAIGIHEYKNLGILPEAISNYLLRMGWSHEDDEIISMDQAIKWFSIKNIGQSPARLDNKKLEFLNNHYISLTEDEVI
LNMIIPIIEKKIGYMLNEVKKGYLLKGLYELKKRTKNLVNLANESLFYVEDVPISIDQEASAIIKDYKHVFSILYNNLSR
ISEKEWNNSILTSTIKNISQNLDIKISNIYHCLRASIVGRMNAPSIIEIMINLQQEECLKRIKYAQNIE

Sequences:

>Translated_469_residues
MSHNVITRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDKKRSSKELIDSIINAMSWLKIPYDGEIVLQSKN
ISRHIEIANQLILNNKAYYCYCSEEEINKEKEEFSKKGLYYKHNCIWKNKNFTIDNLTRVIRLRSPTEGVTSFDDKVYGN
ITVSNTQLDDMVLLRSDNTPTYLLSVVVDDHDMNITHIIRGTDHLTNTARQLLIYNALEWNPPKFAHIPLIHDEDGNKLS
KRHQAIGIHEYKNLGILPEAISNYLLRMGWSHEDDEIISMDQAIKWFSIKNIGQSPARLDNKKLEFLNNHYISLTEDEVI
LNMIIPIIEKKIGYMLNEVKKGYLLKGLYELKKRTKNLVNLANESLFYVEDVPISIDQEASAIIKDYKHVFSILYNNLSR
ISEKEWNNSILTSTIKNISQNLDIKISNIYHCLRASIVGRMNAPSIIEIMINLQQEECLKRIKYAQNIE
>Mature_468_residues
SHNVITRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDKKRSSKELIDSIINAMSWLKIPYDGEIVLQSKNI
SRHIEIANQLILNNKAYYCYCSEEEINKEKEEFSKKGLYYKHNCIWKNKNFTIDNLTRVIRLRSPTEGVTSFDDKVYGNI
TVSNTQLDDMVLLRSDNTPTYLLSVVVDDHDMNITHIIRGTDHLTNTARQLLIYNALEWNPPKFAHIPLIHDEDGNKLSK
RHQAIGIHEYKNLGILPEAISNYLLRMGWSHEDDEIISMDQAIKWFSIKNIGQSPARLDNKKLEFLNNHYISLTEDEVIL
NMIIPIIEKKIGYMLNEVKKGYLLKGLYELKKRTKNLVNLANESLFYVEDVPISIDQEASAIIKDYKHVFSILYNNLSRI
SEKEWNNSILTSTIKNISQNLDIKISNIYHCLRASIVGRMNAPSIIEIMINLQQEECLKRIKYAQNIE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=485, Percent_Identity=30.1030927835052, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=36.480686695279, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI226510925, Length=291, Percent_Identity=31.2714776632302, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=494, Percent_Identity=31.3765182186235, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=328, Percent_Identity=37.5, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324742, Length=220, Percent_Identity=30, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=314, Percent_Identity=24.203821656051, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=30.8943089430894, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_EHRCR (Q2GGL8)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507415.1
- ProteinModelPortal:   Q2GGL8
- SMR:   Q2GGL8
- STRING:   Q2GGL8
- GeneID:   3927900
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0605
- TIGR:   ECH_0605
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- PhylomeDB:   Q2GGL8
- ProtClustDB:   PRK01406
- BioCyc:   ECHA205920:ECH_0605-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54320; Mature: 54189

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNVITRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDKKRSSKELIDSII
CCCCEEEEECCCCCCEEEECCHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHH
NAMSWLKIPYDGEIVLQSKNISRHIEIANQLILNNKAYYCYCSEEEINKEKEEFSKKGLY
HHHHHEECCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECHHHHCHHHHHHHHCCCE
YKHNCIWKNKNFTIDNLTRVIRLRSPTEGVTSFDDKVYGNITVSNTQLDDMVLLRSDNTP
EEEEEEECCCCCCHHHHHHHHEECCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCC
TYLLSVVVDDHDMNITHIIRGTDHLTNTARQLLIYNALEWNPPKFAHIPLIHDEDGNKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHEECCCCCCCCEEEEEEEECCCCCHHH
KRHQAIGIHEYKNLGILPEAISNYLLRMGWSHEDDEIISMDQAIKWFSIKNIGQSPARLD
HHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCC
NKKLEFLNNHYISLTEDEVILNMIIPIIEKKIGYMLNEVKKGYLLKGLYELKKRTKNLVN
CHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANESLFYVEDVPISIDQEASAIIKDYKHVFSILYNNLSRISEKEWNNSILTSTIKNISQ
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NLDIKISNIYHCLRASIVGRMNAPSIIEIMINLQQEECLKRIKYAQNIE
CCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHNVITRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDKKRSSKELIDSII
CCCEEEEECCCCCCEEEECCHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHH
NAMSWLKIPYDGEIVLQSKNISRHIEIANQLILNNKAYYCYCSEEEINKEKEEFSKKGLY
HHHHHEECCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECHHHHCHHHHHHHHCCCE
YKHNCIWKNKNFTIDNLTRVIRLRSPTEGVTSFDDKVYGNITVSNTQLDDMVLLRSDNTP
EEEEEEECCCCCCHHHHHHHHEECCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCC
TYLLSVVVDDHDMNITHIIRGTDHLTNTARQLLIYNALEWNPPKFAHIPLIHDEDGNKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHEECCCCCCCCEEEEEEEECCCCCHHH
KRHQAIGIHEYKNLGILPEAISNYLLRMGWSHEDDEIISMDQAIKWFSIKNIGQSPARLD
HHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCC
NKKLEFLNNHYISLTEDEVILNMIIPIIEKKIGYMLNEVKKGYLLKGLYELKKRTKNLVN
CHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANESLFYVEDVPISIDQEASAIIKDYKHVFSILYNNLSRISEKEWNNSILTSTIKNISQ
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NLDIKISNIYHCLRASIVGRMNAPSIIEIMINLQQEECLKRIKYAQNIE
CCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA