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The map label for this gene is lipB

Identifier: 88657879

GI number: 88657879

Start: 363550

End: 364194

Strand: Direct

Name: lipB

Synonym: ECH_0371

Alternate gene names: 88657879

Gene position: 363550-364194 (Clockwise)

Preceding gene: 88657719

Following gene: 88658020

Centisome position: 30.91

GC content: 31.16

Gene sequence:

>645_bases
ATGTCATATGATTTAATAGAGTGGAAAATAGAGTCTCTACAAATAGATTATCAAGAGGCAATATGCTTTATGCAACAAAG
GATAGAGGATATTTATAATGGAGTGCAAAAGGAATTAGTTTGGTTGCTTGAACATCCTCCGTTGTATACTGCTGGTACTG
GAGCTAAAGTTGAAGATTTACTAGTTGATAATTTATTTCCTGTATATGCTACAAATAGAGGTGGTAAATATACTTATCAT
GGCCCTGGGCAAAGGATTGCTTATGTTATGTTGAATCTGAAAACCAGAAACAAGTGTAATATTAGATTATATGTTGAGAC
TTTAGGCAATTGGATTGTTGAAACTTTAAGTCATTTTTCAGTCAAGTCGTATTTTAATCCTGACTTGATAGGTGTATGGG
TAACTCATGGTGGTACTGAAAAAAAAATTGCTGCATTTGGAATTAGGATAAGGAAGTGGGTAACTTATCATGGAGTATCT
ATAAATATTGATACAAATCTTTCTCATTATTCAGGCATAGTACCATGTGGTATTAAAGATTATGGTATTACTTCTCTTAG
GAAGTTGGGAATTAATATCTCTTATGAAGAATTTGATATTGTACTACAAGAAAAGTTTAATGAAATTTTTTCTAATTTTA
ATTGA

Upstream 100 bases:

>100_bases
AGCTTACTACATCTGCATTTTTGTCGAAGTTATATAATAGTTTTGGTCTATTATTGAGTGTATAGTGTTATGATGATTTT
TTTGATGTGTGATTAAGTCT

Downstream 100 bases:

>100_bases
AAGAACACTGTAATATGTTGAAATTAAGTGGCATATACTCTTCTATCTTGTAATAGCAGCATTTTTAATGCAATCAATGT
AAATTTGGAGTTGGTAAATG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSYDLIEWKIESLQIDYQEAICFMQQRIEDIYNGVQKELVWLLEHPPLYTAGTGAKVEDLLVDNLFPVYATNRGGKYTYH
GPGQRIAYVMLNLKTRNKCNIRLYVETLGNWIVETLSHFSVKSYFNPDLIGVWVTHGGTEKKIAAFGIRIRKWVTYHGVS
INIDTNLSHYSGIVPCGIKDYGITSLRKLGINISYEEFDIVLQEKFNEIFSNFN

Sequences:

>Translated_214_residues
MSYDLIEWKIESLQIDYQEAICFMQQRIEDIYNGVQKELVWLLEHPPLYTAGTGAKVEDLLVDNLFPVYATNRGGKYTYH
GPGQRIAYVMLNLKTRNKCNIRLYVETLGNWIVETLSHFSVKSYFNPDLIGVWVTHGGTEKKIAAFGIRIRKWVTYHGVS
INIDTNLSHYSGIVPCGIKDYGITSLRKLGINISYEEFDIVLQEKFNEIFSNFN
>Mature_213_residues
SYDLIEWKIESLQIDYQEAICFMQQRIEDIYNGVQKELVWLLEHPPLYTAGTGAKVEDLLVDNLFPVYATNRGGKYTYHG
PGQRIAYVMLNLKTRNKCNIRLYVETLGNWIVETLSHFSVKSYFNPDLIGVWVTHGGTEKKIAAFGIRIRKWVTYHGVSI
NIDTNLSHYSGIVPCGIKDYGITSLRKLGINISYEEFDIVLQEKFNEIFSNFN

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=180, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI87081767, Length=168, Percent_Identity=36.3095238095238, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323268, Length=208, Percent_Identity=30.2884615384615, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355483, Length=175, Percent_Identity=37.7142857142857, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_EHRCR (Q2GH92)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507191.1
- ProteinModelPortal:   Q2GH92
- SMR:   Q2GH92
- STRING:   Q2GH92
- GeneID:   3927690
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0371
- TIGR:   ECH_0371
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q2GH92
- ProtClustDB:   PRK14341
- BioCyc:   ECHA205920:ECH_0371-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24686; Mature: 24554

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDLIEWKIESLQIDYQEAICFMQQRIEDIYNGVQKELVWLLEHPPLYTAGTGAKVEDL
CCCCHHEEEEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHH
LVDNLFPVYATNRGGKYTYHGPGQRIAYVMLNLKTRNKCNIRLYVETLGNWIVETLSHFS
HHHCCCEEEEECCCCEEEEECCCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
VKSYFNPDLIGVWVTHGGTEKKIAAFGIRIRKWVTYHGVSINIDTNLSHYSGIVPCGIKD
HHHCCCCCEEEEEEECCCCCCCHHHHHHEEEEEEEEECEEEEEECCHHHHCCCCCCCCCC
YGITSLRKLGINISYEEFDIVLQEKFNEIFSNFN
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYDLIEWKIESLQIDYQEAICFMQQRIEDIYNGVQKELVWLLEHPPLYTAGTGAKVEDL
CCCHHEEEEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHH
LVDNLFPVYATNRGGKYTYHGPGQRIAYVMLNLKTRNKCNIRLYVETLGNWIVETLSHFS
HHHCCCEEEEECCCCEEEEECCCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
VKSYFNPDLIGVWVTHGGTEKKIAAFGIRIRKWVTYHGVSINIDTNLSHYSGIVPCGIKD
HHHCCCCCEEEEEEECCCCCCCHHHHHHEEEEEEEEECEEEEEECCHHHHCCCCCCCCCC
YGITSLRKLGINISYEEFDIVLQEKFNEIFSNFN
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA