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The map label for this gene is fumC [H]

Identifier: 88657861

GI number: 88657861

Start: 367403

End: 368788

Strand: Direct

Name: fumC [H]

Synonym: ECH_0376

Alternate gene names: 88657861

Gene position: 367403-368788 (Clockwise)

Preceding gene: 88658020

Following gene: 88657615

Centisome position: 31.24

GC content: 34.27

Gene sequence:

>1386_bases
ATGAGAAAGGAAAGAGATAGTTTGGGAGAGATAGATGTTCCTGCATGCCATTACTGGGGTGCACAAACACAACGTTCTAT
TGATAATTTTAAGATTGGCTCAGAGAAAATGCCTAAACCTTTAATAAAGGCATTAGGACTTGTTAAATTAGCTGCAGCTC
GTGTCAATATGAAAAATGGTGATATTAGTGAAACAATAGGTAATGCAATTTGTAATGCTGCAAGTGAAATTATAGATGGT
AAGTTTGATGATGAGTTCCCTTTAGTAATTTGGCAAACTGGTTCTGGGACTCAAAGTAATATGAATGTTAATGAAGTAAT
AGCAAATCGTGCAATTGAAATATTAGGTGGAGAAAAAGGTAGTAAATATCCTGTCCATCCAAATGATCATGTGAATTATT
CTCAATCCTCTAATGATACTTTTCCAACAGCAATGCATATTGCGGCTGTTATTGAAACTGAAAATTCATTGTTGCCAAAT
TTGAAAAATTTATATGATGCTCTACATAGTAAGTCTATAGCGTTTCAAAATATTGTTAAAATTGGGCGTACTCACCTTCA
AGATGCTACTCCGTTGACTTTAGGGCAGGTATTTTCTGGGTATGCGTATCAGATTTTACAGGGTATGAGTAGGGTCAAGT
CAGCGTTAGGTCATTTACTTGAGCTAGCTCAAGGTGGTACTGCAGTAGGTACTGGTATTAATTCTAGGAAACAGTTTGAT
GTCCATTTTGCAAGTGAAATAAAAAAATTAACAGGTTTTAATTTTGTTGCTTCTGTTAATAAATTTGAGGCATTAGCTAC
ACATGATGCATTGGTTGAATTTAGTGGAGCTTTAAATGTGCTTGCAGTAAGTTTAATGAAAATAGCTAATGATATTAGGT
TACTTAGTTCAGGTCCAAGATGTGGAATAGGGGAAATTATATTGCCTACTAATGAACCTGGATCTTCTATAATGCCTGGG
AAAGTAAATCCTACCCAGTGTGAAGCTGTTACTATGGTGTGTGCTCAAGTTATGGGGAATCATACAACTGTTACTGTTGC
AGGGTCAAATGGTCATTTTGAGTTGAATGTATTTAAACCAGTGATTATTTATAATGTTTTACAATCTATTAGATTATTGT
CTGACGTTAGCATTAATTTTTCTCACAAGTGCGTTCAGGGGATAAAAGCTGATACAAATCGTATTTCGTTTTTGTTGAGT
CAATCTTTAATGTTGGTAACAGCATTAAACAAACATATAGGTTATGATAATGCGGCAAAAATTGCAAAGACTGCCTTTGA
TGAGGGAATAACGTTAAAAAATGCTGCAGTTAAGTTGAATTTAATAAGTGAAGAGGAGTTTGATAAAATCGTTAATCCAG
AAAATATGATATATCCTACAGGTTAA

Upstream 100 bases:

>100_bases
TTATATAGTGTAGCACTAATATAGTTGTGTTAGTTATGGTGGACATAAATACAAAACTTTATTAATATAATTTATCACAT
GTAGGTGGAAAATTTAGAAT

Downstream 100 bases:

>100_bases
ATATTGTTTAATACAACTTGTTTAATAACTAACAGTGTTGACAGTTAATACGTGTTATTATAACATGAAGTATTATGTTC
TTAATGAAAAGTGATTTTAT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRKERDSLGEIDVPACHYWGAQTQRSIDNFKIGSEKMPKPLIKALGLVKLAAARVNMKNGDISETIGNAICNAASEIIDG
KFDDEFPLVIWQTGSGTQSNMNVNEVIANRAIEILGGEKGSKYPVHPNDHVNYSQSSNDTFPTAMHIAAVIETENSLLPN
LKNLYDALHSKSIAFQNIVKIGRTHLQDATPLTLGQVFSGYAYQILQGMSRVKSALGHLLELAQGGTAVGTGINSRKQFD
VHFASEIKKLTGFNFVASVNKFEALATHDALVEFSGALNVLAVSLMKIANDIRLLSSGPRCGIGEIILPTNEPGSSIMPG
KVNPTQCEAVTMVCAQVMGNHTTVTVAGSNGHFELNVFKPVIIYNVLQSIRLLSDVSINFSHKCVQGIKADTNRISFLLS
QSLMLVTALNKHIGYDNAAKIAKTAFDEGITLKNAAVKLNLISEEEFDKIVNPENMIYPTG

Sequences:

>Translated_461_residues
MRKERDSLGEIDVPACHYWGAQTQRSIDNFKIGSEKMPKPLIKALGLVKLAAARVNMKNGDISETIGNAICNAASEIIDG
KFDDEFPLVIWQTGSGTQSNMNVNEVIANRAIEILGGEKGSKYPVHPNDHVNYSQSSNDTFPTAMHIAAVIETENSLLPN
LKNLYDALHSKSIAFQNIVKIGRTHLQDATPLTLGQVFSGYAYQILQGMSRVKSALGHLLELAQGGTAVGTGINSRKQFD
VHFASEIKKLTGFNFVASVNKFEALATHDALVEFSGALNVLAVSLMKIANDIRLLSSGPRCGIGEIILPTNEPGSSIMPG
KVNPTQCEAVTMVCAQVMGNHTTVTVAGSNGHFELNVFKPVIIYNVLQSIRLLSDVSINFSHKCVQGIKADTNRISFLLS
QSLMLVTALNKHIGYDNAAKIAKTAFDEGITLKNAAVKLNLISEEEFDKIVNPENMIYPTG
>Mature_461_residues
MRKERDSLGEIDVPACHYWGAQTQRSIDNFKIGSEKMPKPLIKALGLVKLAAARVNMKNGDISETIGNAICNAASEIIDG
KFDDEFPLVIWQTGSGTQSNMNVNEVIANRAIEILGGEKGSKYPVHPNDHVNYSQSSNDTFPTAMHIAAVIETENSLLPN
LKNLYDALHSKSIAFQNIVKIGRTHLQDATPLTLGQVFSGYAYQILQGMSRVKSALGHLLELAQGGTAVGTGINSRKQFD
VHFASEIKKLTGFNFVASVNKFEALATHDALVEFSGALNVLAVSLMKIANDIRLLSSGPRCGIGEIILPTNEPGSSIMPG
KVNPTQCEAVTMVCAQVMGNHTTVTVAGSNGHFELNVFKPVIIYNVLQSIRLLSDVSINFSHKCVQGIKADTNRISFLLS
QSLMLVTALNKHIGYDNAAKIAKTAFDEGITLKNAAVKLNLISEEEFDKIVNPENMIYPTG

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=64.7826086956522, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI266458395, Length=63, Percent_Identity=60.3174603174603, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1787896, Length=457, Percent_Identity=59.9562363238512, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=39.1774891774892, Blast_Score=324, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=64.4251626898048, Blast_Score=607, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=64.1104294478528, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=62.6898047722343, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=61.6883116883117, Blast_Score=580, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=61.6883116883117, Blast_Score=579, Evalue=1e-165,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=59.95670995671, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=58.3153347732181, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=52.1645021645022, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 49924; Mature: 49924

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKERDSLGEIDVPACHYWGAQTQRSIDNFKIGSEKMPKPLIKALGLVKLAAARVNMKNG
CCCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
DISETIGNAICNAASEIIDGKFDDEFPLVIWQTGSGTQSNMNVNEVIANRAIEILGGEKG
CHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHEECCCCC
SKYPVHPNDHVNYSQSSNDTFPTAMHIAAVIETENSLLPNLKNLYDALHSKSIAFQNIVK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCCCHHHHHHHHHHCHHHHHHHHH
IGRTHLQDATPLTLGQVFSGYAYQILQGMSRVKSALGHLLELAQGGTAVGTGINSRKQFD
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEE
VHFASEIKKLTGFNFVASVNKFEALATHDALVEFSGALNVLAVSLMKIANDIRLLSSGPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
CGIGEIILPTNEPGSSIMPGKVNPTQCEAVTMVCAQVMGNHTTVTVAGSNGHFELNVFKP
CCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCH
VIIYNVLQSIRLLSDVSINFSHKCVQGIKADTNRISFLLSQSLMLVTALNKHIGYDNAAK
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IAKTAFDEGITLKNAAVKLNLISEEEFDKIVNPENMIYPTG
HHHHHHHCCCEEECCEEEEEECCHHHHHHHCCCCCEEECCC
>Mature Secondary Structure
MRKERDSLGEIDVPACHYWGAQTQRSIDNFKIGSEKMPKPLIKALGLVKLAAARVNMKNG
CCCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
DISETIGNAICNAASEIIDGKFDDEFPLVIWQTGSGTQSNMNVNEVIANRAIEILGGEKG
CHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHEECCCCC
SKYPVHPNDHVNYSQSSNDTFPTAMHIAAVIETENSLLPNLKNLYDALHSKSIAFQNIVK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCCCHHHHHHHHHHCHHHHHHHHH
IGRTHLQDATPLTLGQVFSGYAYQILQGMSRVKSALGHLLELAQGGTAVGTGINSRKQFD
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEE
VHFASEIKKLTGFNFVASVNKFEALATHDALVEFSGALNVLAVSLMKIANDIRLLSSGPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
CGIGEIILPTNEPGSSIMPGKVNPTQCEAVTMVCAQVMGNHTTVTVAGSNGHFELNVFKP
CCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCH
VIIYNVLQSIRLLSDVSINFSHKCVQGIKADTNRISFLLSQSLMLVTALNKHIGYDNAAK
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IAKTAFDEGITLKNAAVKLNLISEEEFDKIVNPENMIYPTG
HHHHHHHCCCEEECCEEEEEECCHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA