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The map label for this gene is hemC

Identifier: 88657743

GI number: 88657743

Start: 709417

End: 710313

Strand: Reverse

Name: hemC

Synonym: ECH_0701

Alternate gene names: 88657743

Gene position: 710313-709417 (Counterclockwise)

Preceding gene: 88658221

Following gene: 88658168

Centisome position: 60.39

GC content: 31.66

Gene sequence:

>897_bases
ATGAATATAAGAATTGGGACGAGAGGAAGTATTTTAGCGATAGCACAGACATTAGAAATTAAAAATCTGTTAAACAGATA
TTTTCCTGAAATTAGTGTTCAAATTGTTAAAATTAAGACTTCTGGAGATATCAATAATCAGGTTCCTCTGAGTGCTATAG
GTGGAAAAAGCTTATTTATAAAGGAAATAGAGGAAGCATTATTAATGGGGAAAGTAGATTTAGCTGTGCATTCAGTAAAA
GACATACCAGCATTTTATTGTGAGGGGTTGATCATTCCTTGTGTGTTAAAACGTAATAGCCCATATGATGTTTTTATTTC
ATCTAAGCATAAAGATATAAAATCACTTCCTCTTAATGCAACCATAGGTACATCTTCTGTGAGAAGAAAAGTACAGTTAA
ATTATTTGCGTCCAGATCTTCAAGTAGTTCCTGTTAGAGGTAATATAGACACTAGAATATTAAAGGCTAATGTTGGTGAA
TTTGATGGTATAGTATTGGCTGAAGCTGGATTAATCAGAATAAATAGATGTGATGTAATTAAAGAAATATTAAGTCCTAA
AATAATGTTAAGTGCAGTAGGACAAGGTGCAATAGGTATTCAATGCAGAGTTGATGATCATAGTATAATAAATAAGATTA
AGGTTTTAAATTGCCATCAGTCTTACGTTTGTGTAATGGCTGAAAGAAGTTTTTTAAAAACAATTAATGGTTCTTGTGAT
ACGCCATTAGCAGCGCTAGCTCAGTACGTTAATAACGATACAATTCATATGTCTTGTATGCTTGCAAATGAAAAAAATAT
GGTATTTGCAAGCTGCTGCTTTAATGAGTGTGATGCGGAAAAATCAGGTATTAATATGGGAAAGAAATTAATGGATGAAT
TGAGTCAGTATTATTAA

Upstream 100 bases:

>100_bases
CTTGATATTGGGTTTTCGTTTGATTTGTTTATAAATGCTGTAGCATGTGGCGTATAGTTTTGTTATTATTGAGCTTTTAT
GAGTATTTTGCTAATTCATT

Downstream 100 bases:

>100_bases
AAATATTAGTATTTTTTATTATTATAGTATTAATTTGCGTTGGTTTCTAAGTAAGAATAACAATTTTTTGTTGTATTATA
TGTTTTGATATACTTGACTT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNIRIGTRGSILAIAQTLEIKNLLNRYFPEISVQIVKIKTSGDINNQVPLSAIGGKSLFIKEIEEALLMGKVDLAVHSVK
DIPAFYCEGLIIPCVLKRNSPYDVFISSKHKDIKSLPLNATIGTSSVRRKVQLNYLRPDLQVVPVRGNIDTRILKANVGE
FDGIVLAEAGLIRINRCDVIKEILSPKIMLSAVGQGAIGIQCRVDDHSIINKIKVLNCHQSYVCVMAERSFLKTINGSCD
TPLAALAQYVNNDTIHMSCMLANEKNMVFASCCFNECDAEKSGINMGKKLMDELSQYY

Sequences:

>Translated_298_residues
MNIRIGTRGSILAIAQTLEIKNLLNRYFPEISVQIVKIKTSGDINNQVPLSAIGGKSLFIKEIEEALLMGKVDLAVHSVK
DIPAFYCEGLIIPCVLKRNSPYDVFISSKHKDIKSLPLNATIGTSSVRRKVQLNYLRPDLQVVPVRGNIDTRILKANVGE
FDGIVLAEAGLIRINRCDVIKEILSPKIMLSAVGQGAIGIQCRVDDHSIINKIKVLNCHQSYVCVMAERSFLKTINGSCD
TPLAALAQYVNNDTIHMSCMLANEKNMVFASCCFNECDAEKSGINMGKKLMDELSQYY
>Mature_298_residues
MNIRIGTRGSILAIAQTLEIKNLLNRYFPEISVQIVKIKTSGDINNQVPLSAIGGKSLFIKEIEEALLMGKVDLAVHSVK
DIPAFYCEGLIIPCVLKRNSPYDVFISSKHKDIKSLPLNATIGTSSVRRKVQLNYLRPDLQVVPVRGNIDTRILKANVGE
FDGIVLAEAGLIRINRCDVIKEILSPKIMLSAVGQGAIGIQCRVDDHSIINKIKVLNCHQSYVCVMAERSFLKTINGSCD
TPLAALAQYVNNDTIHMSCMLANEKNMVFASCCFNECDAEKSGINMGKKLMDELSQYY

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=246, Percent_Identity=39.0243902439024, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI66933009, Length=246, Percent_Identity=39.0243902439024, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI48994974, Length=291, Percent_Identity=37.4570446735395, Blast_Score=211, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319996, Length=262, Percent_Identity=33.969465648855, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI20130425, Length=250, Percent_Identity=39.6, Blast_Score=186, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_EHRCR (Q2GGC7)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507506.1
- ProteinModelPortal:   Q2GGC7
- SMR:   Q2GGC7
- STRING:   Q2GGC7
- GeneID:   3927748
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0701
- TIGR:   ECH_0701
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   DADILEM
- PhylomeDB:   Q2GGC7
- ProtClustDB:   PRK00072
- BioCyc:   ECHA205920:ECH_0701-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32920; Mature: 32920

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRIGTRGSILAIAQTLEIKNLLNRYFPEISVQIVKIKTSGDINNQVPLSAIGGKSLFI
CEEEECCCCCEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEECCCCHHHH
KEIEEALLMGKVDLAVHSVKDIPAFYCEGLIIPCVLKRNSPYDVFISSKHKDIKSLPLNA
HHHHHHHHHCHHHHHHHHHHCCHHHHHCCEEEEEEEECCCCEEEEEECCCCCHHCCCCCE
TIGTSSVRRKVQLNYLRPDLQVVPVRGNIDTRILKANVGEFDGIVLAEAGLIRINRCDVI
EECHHHHHHEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEECCCEEEEHHHHHH
KEILSPKIMLSAVGQGAIGIQCRVDDHSIINKIKVLNCHQSYVCVMAERSFLKTINGSCD
HHHHCCCEEEHHCCCCCEEEEEEECCHHHHHHEEEEEECCCEEEEEHHHHHHHHHCCCCC
TPLAALAQYVNNDTIHMSCMLANEKNMVFASCCFNECDAEKSGINMGKKLMDELSQYY
CHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIRIGTRGSILAIAQTLEIKNLLNRYFPEISVQIVKIKTSGDINNQVPLSAIGGKSLFI
CEEEECCCCCEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEECCCCHHHH
KEIEEALLMGKVDLAVHSVKDIPAFYCEGLIIPCVLKRNSPYDVFISSKHKDIKSLPLNA
HHHHHHHHHCHHHHHHHHHHCCHHHHHCCEEEEEEEECCCCEEEEEECCCCCHHCCCCCE
TIGTSSVRRKVQLNYLRPDLQVVPVRGNIDTRILKANVGEFDGIVLAEAGLIRINRCDVI
EECHHHHHHEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEECCCEEEEHHHHHH
KEILSPKIMLSAVGQGAIGIQCRVDDHSIINKIKVLNCHQSYVCVMAERSFLKTINGSCD
HHHHCCCEEEHHCCCCCEEEEEEECCHHHHHHEEEEEECCCEEEEEHHHHHHHHHCCCCC
TPLAALAQYVNNDTIHMSCMLANEKNMVFASCCFNECDAEKSGINMGKKLMDELSQYY
CHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA