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The map label for this gene is topA [H]

Identifier: 88657600

GI number: 88657600

Start: 754252

End: 756714

Strand: Reverse

Name: topA [H]

Synonym: ECH_0750

Alternate gene names: 88657600

Gene position: 756714-754252 (Counterclockwise)

Preceding gene: 88658205

Following gene: 88657812

Centisome position: 64.33

GC content: 31.34

Gene sequence:

>2463_bases
ATGAGTTTAATTATAGTAGAATCCCCATCTAAAGCAAAAACTATAAGTAAATATTTAGGTAGCAAGTATAAAGTTGTTGC
ATCATTTGGGCATATTAGGGATTTTCCAGCAAAAAGTGGTTCTGTTGATCCGGATAAAGATTTCGCTATGACTTATGAAA
TTATTAGTAAGTCAGAAAAATATGTAAATAAGTTAATTCAGGTAGCTAATAAGGAAACACAAGGAATTTATCTTGCAACT
GATCCAGATCGTGAGGGTGAAGCAATAGCATGGCATATTATAGAAGTATTGAAGGAAAATAATGCTATTGATGATAATGT
TGTAATTAATAGAATGGTTTTTAATGAGGTAACTAAAAGTGCTATTCTTGAATCTATAAAACGCCCAAGAAGTATCAATA
TGGATCTTGTGTATGCACAGCAAGCACGTAGAGCATTAGATTATTTAGTTGGTTTTACGTTATCTCCTTTATTATGGAGA
AAACTTCCGGGTAGTAAATCTGCTGGTAGAGTTCAATCTGTTGCTTTAAGGTTAATATGTGACAGGGAAAGTGATATTGA
AAAGTTCATTACACAAGAATATTGGGATATTGAGGCTAAATGTTGCAATACAGAAGGTAAGCAGTTTTCTGCATTTTTAA
GTTGTTATGCTGGTAAAAAATTAGAGAAGTTTGATATTAAGAATGAAGAAGATGCACAAAAGTTGTCAGAAGAAGTAAGA
TCTCGTAGTTATTCTGTTATAAATGTTGAGAAAAAGCATACAAAGCGTAATCCTTATCCTCCTTTTATTACATCAAGTTT
ACAGCAAGAAGCATCAACAAAGCTTGGATTTACTGCAAAAAATACTATGCTTATTGCTCAAAAACTGTATGAAGGAATTG
ATGTTGGAGGGGAAATTGTTGGACTCATTACTTATATGAGGACGGATGGATTTTATATTGCAGATGAAGCATTAGAGCAT
ATTAGGAGTATCATTGTTTCTATGTTTGGTAAAGAATATTTACCGGAATCTGCTCGCAAGTATGTGAAAAAGGTAAAAAA
TGCTCAAGAAGCACATGAAGCAATTAGACCAACGAATATTACAATTACTCCTGGTAGTTTATCAAATTATCTCACTCCAG
AGCAACTCAAGCTTTATGATCTTATATGGAAAAGGACTGTTGCAAGTCAGATGAATTCTGCTGTAATTGATCAGGTTATA
GTAGAATTAGGGTCGCTGGATAAAGTAGTAACTTTGCGAGCCACAGGTTCTTCCTTGTTTTTTGATGGATTTTATAAGGT
GTATGGTCATGGTGAGGATGATGATAGTAAAAATAATATGCTACCGTTACTTCAAAAAAATGATAAATGCCCTTTAGATG
AAGTGGTACCTCATCAACATTTTACTAATCCTCCTGCTAGATATAATGAAGCAAGTTTAGTGAAGAAAATGGAGGAAATA
GGGATAGGAAGGCCTTCTACTTATGCTTCTATTATTTCTGTATTGCAAGATAGACAGTATGTTATACTAAATCAAAAAAG
ATTTTTTCCAACAGAGAGAGGTAGAATAGTAAATGTTTTTCTTGTTAACTTTTTTAGTCGCTATGTAGAATATGATTTTA
CTGCTGACCTTGAAGAAAAGTTGGATTTAATCTCTAATGGGAAGATTAATTGGAAGGAAGTACTACATCAATTCTGGTTT
CTGTTTATTGATAATGTTAATTCAGTAAAAAAACTAGAATTTACTGAAGTATTAAAAGTTATTAGTTGTGAGATGGAAAA
TTATATTTTTTCTTCTGATAAATCTGAATCTAGTAGAGTATGTCCTGTATGTAATGATGGAACATTAGAGCTTAATATTA
GTAAGAATGGTATTTTTTTAGGATGTTCTAAATATCCAGAGTGTAAGTATACAAAAAATGTTGGAAGTGGTATTGTTGAT
GATACAGACAATACACAATATCCTAAAATATTAGGTATTGATGCTGCAACAGGACAAGAAATATATCTTAAAAAAGGTCC
GTATGGTCTCTATTTACAGATTGGTAATGATAAAAGTGGTAAACGTGCAGCTATTCCAAAAAATATAGATGCTAATTTAT
TAGATTTGCAAATGGCAGAAAAAATATTAAGTTTACCTATAAGCTTAGGTACTCATCCGGATACTGGTCAAGAAATTAAG
TTAGGACTTGGTAAGTTTGGTGCATACATATTGTATGGTGGTAAGTATTTTTCAGTAAAAAATGAGCATAATTTTTTAGA
TATTAGCATTGAAAACGCTATTAATATTATTGCGAATAATTCTACAAGTGGGGTTATAAAGTCTCTGGGTATCACTGAAG
ATAATAAAGAAGTATTCTTATGTAAAGGAAGATATGGATTCTATTTAAAATATGATAAGTTGAATGTTGCTTTGAAGAAA
GATACAGATATTGAACAAATATCTTTATCGGATGCTATTAAGCTCATATCAGAAAAATTATAA

Upstream 100 bases:

>100_bases
AATTTAAAATTCTTTTCACAATTATCGTTAGTAATAGTGCTAATCTATTGATAGAGCATAGTAAGAAGGATTTGTTAAAT
GGTAATCTTAGGGTGTAATA

Downstream 100 bases:

>100_bases
CTAGAAATATAAAGTTATGTATAAATAGTAATATAGTACTGCTAAGTGATAATTTAATCTTTTAGTTCTTGACATTATAT
ATAAATACAATAATGACAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 820; Mature: 819

Protein sequence:

>820_residues
MSLIIVESPSKAKTISKYLGSKYKVVASFGHIRDFPAKSGSVDPDKDFAMTYEIISKSEKYVNKLIQVANKETQGIYLAT
DPDREGEAIAWHIIEVLKENNAIDDNVVINRMVFNEVTKSAILESIKRPRSINMDLVYAQQARRALDYLVGFTLSPLLWR
KLPGSKSAGRVQSVALRLICDRESDIEKFITQEYWDIEAKCCNTEGKQFSAFLSCYAGKKLEKFDIKNEEDAQKLSEEVR
SRSYSVINVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIVGLITYMRTDGFYIADEALEH
IRSIIVSMFGKEYLPESARKYVKKVKNAQEAHEAIRPTNITITPGSLSNYLTPEQLKLYDLIWKRTVASQMNSAVIDQVI
VELGSLDKVVTLRATGSSLFFDGFYKVYGHGEDDDSKNNMLPLLQKNDKCPLDEVVPHQHFTNPPARYNEASLVKKMEEI
GIGRPSTYASIISVLQDRQYVILNQKRFFPTERGRIVNVFLVNFFSRYVEYDFTADLEEKLDLISNGKINWKEVLHQFWF
LFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVD
DTDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIK
LGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFLCKGRYGFYLKYDKLNVALKK
DTDIEQISLSDAIKLISEKL

Sequences:

>Translated_820_residues
MSLIIVESPSKAKTISKYLGSKYKVVASFGHIRDFPAKSGSVDPDKDFAMTYEIISKSEKYVNKLIQVANKETQGIYLAT
DPDREGEAIAWHIIEVLKENNAIDDNVVINRMVFNEVTKSAILESIKRPRSINMDLVYAQQARRALDYLVGFTLSPLLWR
KLPGSKSAGRVQSVALRLICDRESDIEKFITQEYWDIEAKCCNTEGKQFSAFLSCYAGKKLEKFDIKNEEDAQKLSEEVR
SRSYSVINVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIVGLITYMRTDGFYIADEALEH
IRSIIVSMFGKEYLPESARKYVKKVKNAQEAHEAIRPTNITITPGSLSNYLTPEQLKLYDLIWKRTVASQMNSAVIDQVI
VELGSLDKVVTLRATGSSLFFDGFYKVYGHGEDDDSKNNMLPLLQKNDKCPLDEVVPHQHFTNPPARYNEASLVKKMEEI
GIGRPSTYASIISVLQDRQYVILNQKRFFPTERGRIVNVFLVNFFSRYVEYDFTADLEEKLDLISNGKINWKEVLHQFWF
LFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVD
DTDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIK
LGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFLCKGRYGFYLKYDKLNVALKK
DTDIEQISLSDAIKLISEKL
>Mature_819_residues
SLIIVESPSKAKTISKYLGSKYKVVASFGHIRDFPAKSGSVDPDKDFAMTYEIISKSEKYVNKLIQVANKETQGIYLATD
PDREGEAIAWHIIEVLKENNAIDDNVVINRMVFNEVTKSAILESIKRPRSINMDLVYAQQARRALDYLVGFTLSPLLWRK
LPGSKSAGRVQSVALRLICDRESDIEKFITQEYWDIEAKCCNTEGKQFSAFLSCYAGKKLEKFDIKNEEDAQKLSEEVRS
RSYSVINVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIVGLITYMRTDGFYIADEALEHI
RSIIVSMFGKEYLPESARKYVKKVKNAQEAHEAIRPTNITITPGSLSNYLTPEQLKLYDLIWKRTVASQMNSAVIDQVIV
ELGSLDKVVTLRATGSSLFFDGFYKVYGHGEDDDSKNNMLPLLQKNDKCPLDEVVPHQHFTNPPARYNEASLVKKMEEIG
IGRPSTYASIISVLQDRQYVILNQKRFFPTERGRIVNVFLVNFFSRYVEYDFTADLEEKLDLISNGKINWKEVLHQFWFL
FIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVDD
TDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIKL
GLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFLCKGRYGFYLKYDKLNVALKKD
TDIEQISLSDAIKLISEKL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=602, Percent_Identity=25.0830564784053, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI4507635, Length=629, Percent_Identity=22.0985691573927, Blast_Score=101, Evalue=3e-21,
Organism=Escherichia coli, GI1787529, Length=668, Percent_Identity=41.3173652694611, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1788061, Length=652, Percent_Identity=24.3865030674847, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=567, Percent_Identity=23.4567901234568, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=539, Percent_Identity=23.9332096474954, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=493, Percent_Identity=23.7322515212982, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585251, Length=632, Percent_Identity=24.2088607594937, Blast_Score=116, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 92568; Mature: 92437

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIIVESPSKAKTISKYLGSKYKVVASFGHIRDFPAKSGSVDPDKDFAMTYEIISKSEK
CEEEEEECCCHHHHHHHHHCCCEEEEEHHCCHHCCCCCCCCCCCCCCHHHHHHHHHCCHH
YVNKLIQVANKETQGIYLATDPDREGEAIAWHIIEVLKENNAIDDNVVINRMVFNEVTKS
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AILESIKRPRSINMDLVYAQQARRALDYLVGFTLSPLLWRKLPGSKSAGRVQSVALRLIC
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
DRESDIEKFITQEYWDIEAKCCNTEGKQFSAFLSCYAGKKLEKFDIKNEEDAQKLSEEVR
CCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHH
SRSYSVINVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIV
HCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCHHH
GLITYMRTDGFYIADEALEHIRSIIVSMFGKEYLPESARKYVKKVKNAQEAHEAIRPTNI
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEE
TITPGSLSNYLTPEQLKLYDLIWKRTVASQMNSAVIDQVIVELGSLDKVVTLRATGSSLF
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
FDGFYKVYGHGEDDDSKNNMLPLLQKNDKCPLDEVVPHQHFTNPPARYNEASLVKKMEEI
ECCCCEEECCCCCCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
GIGRPSTYASIISVLQDRQYVILNQKRFFPTERGRIVNVFLVNFFSRYVEYDFTADLEEK
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHH
LDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRV
HHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
CPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQE
CEEECCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
IYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIK
EEEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
ECHHCCCEEEEECCEEEEECCCCCEEEEEHHCEEEEEECCCCCHHHHHCCCCCCCCEEEE
CKGRYGFYLKYDKLNVALKKDTDIEQISLSDAIKLISEKL
EECCCEEEEEEECEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLIIVESPSKAKTISKYLGSKYKVVASFGHIRDFPAKSGSVDPDKDFAMTYEIISKSEK
EEEEEECCCHHHHHHHHHCCCEEEEEHHCCHHCCCCCCCCCCCCCCHHHHHHHHHCCHH
YVNKLIQVANKETQGIYLATDPDREGEAIAWHIIEVLKENNAIDDNVVINRMVFNEVTKS
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AILESIKRPRSINMDLVYAQQARRALDYLVGFTLSPLLWRKLPGSKSAGRVQSVALRLIC
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
DRESDIEKFITQEYWDIEAKCCNTEGKQFSAFLSCYAGKKLEKFDIKNEEDAQKLSEEVR
CCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHH
SRSYSVINVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIV
HCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCHHH
GLITYMRTDGFYIADEALEHIRSIIVSMFGKEYLPESARKYVKKVKNAQEAHEAIRPTNI
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEE
TITPGSLSNYLTPEQLKLYDLIWKRTVASQMNSAVIDQVIVELGSLDKVVTLRATGSSLF
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
FDGFYKVYGHGEDDDSKNNMLPLLQKNDKCPLDEVVPHQHFTNPPARYNEASLVKKMEEI
ECCCCEEECCCCCCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
GIGRPSTYASIISVLQDRQYVILNQKRFFPTERGRIVNVFLVNFFSRYVEYDFTADLEEK
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHH
LDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRV
HHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
CPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQE
CEEECCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
IYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIK
EEEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFL
ECHHCCCEEEEECCEEEEECCCCCEEEEEHHCEEEEEECCCCCHHHHHCCCCCCCCEEEE
CKGRYGFYLKYDKLNVALKKDTDIEQISLSDAIKLISEKL
EECCCEEEEEEECEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]