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The map label for this gene is rnhB

Identifier: 88608875

GI number: 88608875

Start: 621093

End: 621680

Strand: Direct

Name: rnhB

Synonym: NSE_0701

Alternate gene names: 88608875

Gene position: 621093-621680 (Clockwise)

Preceding gene: 88608806

Following gene: 88608533

Centisome position: 72.3

GC content: 39.46

Gene sequence:

>588_bases
ATGGTATACAGAAAATCAGAAAATGGACTCATCGCAGGAGTAGATGAGGTAGGAAGAGGTTGCTTAGCTGGACCGGTCGT
GGCATGTGCAGTTATTTTAACAGAAAATCTAGCAATTCCAGGGGACTCAAAAGTCCTTTCACGCGCTGTTAGGCTCTCCT
GGTATGAGGAAATTATTAGTAACTCTTATAATGCTATAGGAATGAGCAGTGTGAAAGAAATAGAAACAATGAACATTCTG
AATGCAACCTTGCTGGCCATGGAAAGAGCACTTGAAAGACTTCCTGTAGTACCAACAACAGTACTCATAGACGGTAGAGA
TATCATTAATAATGCAGCAAAATATAAAGAAGTAAAAAGTGTAGTCTCTGGTGATAAGATTTATGGAGAAATAAAGGCTG
CCTCAATTGTTGCAAAGGTTACTCGGGATAGGTTGATGGAAGGATTAGACCTGACATATCCATATTACCGTTGGAAGTTT
AACAAAGGTTATGGAACAAAGGAACACCTTGAAGCACTAGCAAAATACGGAGTTACAGATCACCATAGAAGGAAATTTGC
TCCAGTAAAGAGGATGCTTCTTAGATAG

Upstream 100 bases:

>100_bases
TTTAAAATCACTCTCTGTAAAGTAAAATCACGAAGTTACTATACTCTGAACACTCACAAATTTGTCATTGCGAAAATAGC
TCAATACCCAAATCTAGTAC

Downstream 100 bases:

>100_bases
AATGAAAATAAAAAAGAGAAGAAGTGTGCTTCGTGATTTTGTTTTGAGACACTTACCAATCGTAAAAATTGAACAATTCC
GCTGCTGCTGCGGCGAAGTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MVYRKSENGLIAGVDEVGRGCLAGPVVACAVILTENLAIPGDSKVLSRAVRLSWYEEIISNSYNAIGMSSVKEIETMNIL
NATLLAMERALERLPVVPTTVLIDGRDIINNAAKYKEVKSVVSGDKIYGEIKAASIVAKVTRDRLMEGLDLTYPYYRWKF
NKGYGTKEHLEALAKYGVTDHHRRKFAPVKRMLLR

Sequences:

>Translated_195_residues
MVYRKSENGLIAGVDEVGRGCLAGPVVACAVILTENLAIPGDSKVLSRAVRLSWYEEIISNSYNAIGMSSVKEIETMNIL
NATLLAMERALERLPVVPTTVLIDGRDIINNAAKYKEVKSVVSGDKIYGEIKAASIVAKVTRDRLMEGLDLTYPYYRWKF
NKGYGTKEHLEALAKYGVTDHHRRKFAPVKRMLLR
>Mature_195_residues
MVYRKSENGLIAGVDEVGRGCLAGPVVACAVILTENLAIPGDSKVLSRAVRLSWYEEIISNSYNAIGMSSVKEIETMNIL
NATLLAMERALERLPVVPTTVLIDGRDIINNAAKYKEVKSVVSGDKIYGEIKAASIVAKVTRDRLMEGLDLTYPYYRWKF
NKGYGTKEHLEALAKYGVTDHHRRKFAPVKRMLLR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=197, Percent_Identity=49.238578680203, Blast_Score=160, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_NEOSM (Q2GD69)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506577.1
- ProteinModelPortal:   Q2GD69
- SMR:   Q2GD69
- STRING:   Q2GD69
- GeneID:   3931613
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0701
- TIGR:   NSE_0701
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q2GD69
- ProtClustDB:   CLSK2528202
- BioCyc:   NSEN222891:NSE_0701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21733; Mature: 21733

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRKSENGLIAGVDEVGRGCLAGPVVACAVILTENLAIPGDSKVLSRAVRLSWYEEIIS
CCEEECCCCCEECHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
NSYNAIGMSSVKEIETMNILNATLLAMERALERLPVVPTTVLIDGRDIINNAAKYKEVKS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHH
VVSGDKIYGEIKAASIVAKVTRDRLMEGLDLTYPYYRWKFNKGYGTKEHLEALAKYGVTD
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCH
HHRRKFAPVKRMLLR
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVYRKSENGLIAGVDEVGRGCLAGPVVACAVILTENLAIPGDSKVLSRAVRLSWYEEIIS
CCEEECCCCCEECHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
NSYNAIGMSSVKEIETMNILNATLLAMERALERLPVVPTTVLIDGRDIINNAAKYKEVKS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHH
VVSGDKIYGEIKAASIVAKVTRDRLMEGLDLTYPYYRWKFNKGYGTKEHLEALAKYGVTD
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCH
HHRRKFAPVKRMLLR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA