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The map label for this gene is folD

Identifier: 88608844

GI number: 88608844

Start: 729743

End: 730639

Strand: Direct

Name: folD

Synonym: NSE_0821

Alternate gene names: 88608844

Gene position: 729743-730639 (Clockwise)

Preceding gene: 88608014

Following gene: 88608138

Centisome position: 84.95

GC content: 41.58

Gene sequence:

>897_bases
ATGACACAACTCATTGATGGTTTCCGAATTGCCGCAGGTATCTGTGAATCTATTGCAACTTCTGTAGCACACCTCAGGAA
AAACAATGGCTTAGTACCTAGTCTGAGAGTCATCATTGTTGGTGAAAACCCGGCAAGTCAAGTGTATGTTCGAAGTAAAC
AGAAAAAAGCAGAGTCGCTTGGTATAGACGCAGAGACTATAGCACTACCGAAAGATACAAGTGAGGTAGAACTAATAAGG
TTAGTAAACTCCTTGAACGACGACACCAGCATAAATGCTATACTCGTCCAACTACCATTGCCTCCTCATATTAATCCGAC
CAGAATTATTGAATCAATTGATTCAAAAAAAGACGTTGATTGTTTCCATCCTGAGAATGTGGGCAGACTAGCTCTCAGTA
AAGATGCGTTGTTAAAACCATGTACCCCAGCAGGCTCTCTATATCTAATAAAATCGGCTTTGGGAGACAATTTGTCTGGT
ATGGATGCCGTTGTCATAGGGCGTTCGAACATTGTTGGTAAGCCAATGGCGATGCTTCTGTTGCATGAAAATTGCACTAT
CACGCTCACACACTCTAAAACTAGGAATATACAAGAAAAAACCAGACAAGCGGATATCGTCATCTCAGCTGTTGGCATAC
CGCATTTTGTAAAAAAAGACTTCATCAAGCCCGGAGCGACTGTTATAGATGTCGGAATGAATAAAGTGGATGGTAAATTA
GTCGGCGACGTTGATTTTGAAGACGTTCTTCAAAAAGTGAAGTTCATTACACCTGTACCAAGAGGCGTCGGTCCAATGAC
AATCGCTTTTCTCATGCTAAACACGGTAATTACGACATGCTTGCAAAACAAGTTGGAGCATCAAGAGGTGATTGGAACTG
TAATCCAGAAACTCTAG

Upstream 100 bases:

>100_bases
TTTGCAAGGGAAAATCGTGAAGATTTGCTCTATGACAAAAAAAAATTACTCGATAATGGCAGTAAATGGGAAAGTGCATT
AATTAAGATGCTCAATAGAA

Downstream 100 bases:

>100_bases
CGCAGGCCAATTGATTGACACCACAAACCTAAAGCAGAGCGAGAGACCGGATGGGGTGGGATTCGAACCCACGGTATGCT
TCCACATACGGCAGTTTTCA

Product: FolD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTQLIDGFRIAAGICESIATSVAHLRKNNGLVPSLRVIIVGENPASQVYVRSKQKKAESLGIDAETIALPKDTSEVELIR
LVNSLNDDTSINAILVQLPLPPHINPTRIIESIDSKKDVDCFHPENVGRLALSKDALLKPCTPAGSLYLIKSALGDNLSG
MDAVVIGRSNIVGKPMAMLLLHENCTITLTHSKTRNIQEKTRQADIVISAVGIPHFVKKDFIKPGATVIDVGMNKVDGKL
VGDVDFEDVLQKVKFITPVPRGVGPMTIAFLMLNTVITTCLQNKLEHQEVIGTVIQKL

Sequences:

>Translated_298_residues
MTQLIDGFRIAAGICESIATSVAHLRKNNGLVPSLRVIIVGENPASQVYVRSKQKKAESLGIDAETIALPKDTSEVELIR
LVNSLNDDTSINAILVQLPLPPHINPTRIIESIDSKKDVDCFHPENVGRLALSKDALLKPCTPAGSLYLIKSALGDNLSG
MDAVVIGRSNIVGKPMAMLLLHENCTITLTHSKTRNIQEKTRQADIVISAVGIPHFVKKDFIKPGATVIDVGMNKVDGKL
VGDVDFEDVLQKVKFITPVPRGVGPMTIAFLMLNTVITTCLQNKLEHQEVIGTVIQKL
>Mature_297_residues
TQLIDGFRIAAGICESIATSVAHLRKNNGLVPSLRVIIVGENPASQVYVRSKQKKAESLGIDAETIALPKDTSEVELIRL
VNSLNDDTSINAILVQLPLPPHINPTRIIESIDSKKDVDCFHPENVGRLALSKDALLKPCTPAGSLYLIKSALGDNLSGM
DAVVIGRSNIVGKPMAMLLLHENCTITLTHSKTRNIQEKTRQADIVISAVGIPHFVKKDFIKPGATVIDVGMNKVDGKLV
GDVDFEDVLQKVKFITPVPRGVGPMTIAFLMLNTVITTCLQNKLEHQEVIGTVIQKL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=304, Percent_Identity=46.0526315789474, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI222136639, Length=286, Percent_Identity=42.6573426573427, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.1780821917808, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI36796743, Length=216, Percent_Identity=27.3148148148148, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=48.4098939929329, Blast_Score=250, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=285, Percent_Identity=42.1052631578947, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=45.3287197231834, Blast_Score=236, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=42.7609427609428, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=316, Percent_Identity=25.6329113924051, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62472483, Length=285, Percent_Identity=45.6140350877193, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45551871, Length=285, Percent_Identity=45.6140350877193, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=45.7746478873239, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=45.7746478873239, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=43.4931506849315, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=43.4931506849315, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_NEOSM (Q2GCV3)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506693.1
- ProteinModelPortal:   Q2GCV3
- SMR:   Q2GCV3
- STRING:   Q2GCV3
- GeneID:   3931653
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0821
- TIGR:   NSE_0821
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- PhylomeDB:   Q2GCV3
- BioCyc:   NSEN222891:NSE_0821-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32304; Mature: 32173

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLIDGFRIAAGICESIATSVAHLRKNNGLVPSLRVIIVGENPASQVYVRSKQKKAESL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHEEHHHHHHHHHC
GIDAETIALPKDTSEVELIRLVNSLNDDTSINAILVQLPLPPHINPTRIIESIDSKKDVD
CCCHHEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
CFHPENVGRLALSKDALLKPCTPAGSLYLIKSALGDNLSGMDAVVIGRSNIVGKPMAMLL
CCCCCCCCCEEECCCCCCCCCCCCCCCEEEHHHHCCCCCCCEEEEEECCCCCCCCCEEEE
LHENCTITLTHSKTRNIQEKTRQADIVISAVGIPHFVKKDFIKPGATVIDVGMNKVDGKL
EECCCEEEEECCCCCCHHHHHHHHCEEEEECCCCHHHHHHHCCCCCEEEECCCCCCCCEE
VGDVDFEDVLQKVKFITPVPRGVGPMTIAFLMLNTVITTCLQNKLEHQEVIGTVIQKL
ECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TQLIDGFRIAAGICESIATSVAHLRKNNGLVPSLRVIIVGENPASQVYVRSKQKKAESL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHEEHHHHHHHHHC
GIDAETIALPKDTSEVELIRLVNSLNDDTSINAILVQLPLPPHINPTRIIESIDSKKDVD
CCCHHEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
CFHPENVGRLALSKDALLKPCTPAGSLYLIKSALGDNLSGMDAVVIGRSNIVGKPMAMLL
CCCCCCCCCEEECCCCCCCCCCCCCCCEEEHHHHCCCCCCCEEEEEECCCCCCCCCEEEE
LHENCTITLTHSKTRNIQEKTRQADIVISAVGIPHFVKKDFIKPGATVIDVGMNKVDGKL
EECCCEEEEECCCCCCHHHHHHHHCEEEEECCCCHHHHHHHCCCCCEEEECCCCCCCCEE
VGDVDFEDVLQKVKFITPVPRGVGPMTIAFLMLNTVITTCLQNKLEHQEVIGTVIQKL
ECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA