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The map label for this gene is hemC [H]

Identifier: 88608800

GI number: 88608800

Start: 315148

End: 316008

Strand: Direct

Name: hemC [H]

Synonym: NSE_0385

Alternate gene names: 88608800

Gene position: 315148-316008 (Clockwise)

Preceding gene: 88608325

Following gene: 88608027

Centisome position: 36.69

GC content: 41.93

Gene sequence:

>861_bases
ATGTTGTCTCTAAAGGTTGGGACGCGTTGTTCGCCATTAGCACTGATTCAGGCACGTCTCGTAGAGAGAGCTCTTGCTCC
CTATTGTGCAAGAACTGATTTAGTCGAAGTAAAGACCTCTGGTGATATAGTCAGTGATGTTCCTTTAACAGAGGTAGGTG
GTAAAGCTCTTTTTCTTAAGGAGCTGGAAGAAAAGCTTCTCACAGGTGAAATAGATATAGCAGTCCATTCAATGAAGGAT
GTACCTGCGTTTTATCATGATGATCTTGAAGTTGTTCCTGTACTCAAGAGGGCTATTCCTAACGATGTTTTTGTTTCTTT
TCGATACCCGAATATGTTGTCTCTGCCAAATGGGGCAGTGATTGGAACTTGTGCTCCACGCAGGGTAGTACAACTAAATC
AAAGATTTCGTGTTATCCCTTTAAGGGGCAATATTGCTACTAGGATAGAAAAAGCTAAAAACTTAGACGGGATAATCCTC
GCCTTCTGTGCTTTAGAAAGACTTGGTTTACGCAGAATGATTTCAGAGGTTATACACGAAAACGTTATGCTTCCGGGTGT
TGCTCAGGGTGCACTTTGTGTTGAATTTAGGAAAAAAGATCGTTTTCTTAGGGAATTGATTCTAAACATTTCTGATAGAG
AGACTATCATTTGTACTGCAGCTGAGCGTGCTTTCCTCGAAGAGATCAATGGTGATTGCAAAACCGCTTTAGGTGCGCTC
GCTATAGTGAAAAACAATATTTTGTATTTCACAGGGATGCTGGCTAAAGACAACAGACCACGTTACTTTAGGACTTCGGG
ATCCTGTTTGAATGCGAAACAAGTTGGTAGAAGGGCTGCTCTGGCGCTTTTGCGCATGTAA

Upstream 100 bases:

>100_bases
GCTAGAGGATTTGTATTGGATGTAATAGAATCGAGTAGTTGGTTGAAGCGTTTTTTTATGGAGCGTGCTGCCGGAAAAGG
TCTGTAGTGATCAATGGGAT

Downstream 100 bases:

>100_bases
AATGCTATCTTAGAACTTATTTTTGCAAGTGTTGCCATTTCTATTGTTCTGAAGGACGTCCAGGCAAAGCTTGCCTACTT
TTACGACATGTTATTCATTA

Product: putative porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLSLKVGTRCSPLALIQARLVERALAPYCARTDLVEVKTSGDIVSDVPLTEVGGKALFLKELEEKLLTGEIDIAVHSMKD
VPAFYHDDLEVVPVLKRAIPNDVFVSFRYPNMLSLPNGAVIGTCAPRRVVQLNQRFRVIPLRGNIATRIEKAKNLDGIIL
AFCALERLGLRRMISEVIHENVMLPGVAQGALCVEFRKKDRFLRELILNISDRETIICTAAERAFLEEINGDCKTALGAL
AIVKNNILYFTGMLAKDNRPRYFRTSGSCLNAKQVGRRAALALLRM

Sequences:

>Translated_286_residues
MLSLKVGTRCSPLALIQARLVERALAPYCARTDLVEVKTSGDIVSDVPLTEVGGKALFLKELEEKLLTGEIDIAVHSMKD
VPAFYHDDLEVVPVLKRAIPNDVFVSFRYPNMLSLPNGAVIGTCAPRRVVQLNQRFRVIPLRGNIATRIEKAKNLDGIIL
AFCALERLGLRRMISEVIHENVMLPGVAQGALCVEFRKKDRFLRELILNISDRETIICTAAERAFLEEINGDCKTALGAL
AIVKNNILYFTGMLAKDNRPRYFRTSGSCLNAKQVGRRAALALLRM
>Mature_286_residues
MLSLKVGTRCSPLALIQARLVERALAPYCARTDLVEVKTSGDIVSDVPLTEVGGKALFLKELEEKLLTGEIDIAVHSMKD
VPAFYHDDLEVVPVLKRAIPNDVFVSFRYPNMLSLPNGAVIGTCAPRRVVQLNQRFRVIPLRGNIATRIEKAKNLDGIIL
AFCALERLGLRRMISEVIHENVMLPGVAQGALCVEFRKKDRFLRELILNISDRETIICTAAERAFLEEINGDCKTALGAL
AIVKNNILYFTGMLAKDNRPRYFRTSGSCLNAKQVGRRAALALLRM

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps [H]

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family [H]

Homologues:

Organism=Homo sapiens, GI20149500, Length=263, Percent_Identity=35.361216730038, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI66933009, Length=263, Percent_Identity=35.361216730038, Blast_Score=151, Evalue=7e-37,
Organism=Escherichia coli, GI48994974, Length=290, Percent_Identity=38.2758620689655, Blast_Score=177, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6319996, Length=254, Percent_Identity=33.8582677165354, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI20130425, Length=258, Percent_Identity=34.4961240310078, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418 [H]

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC [H]

EC number: =2.5.1.61 [H]

Molecular weight: Translated: 31775; Mature: 31775

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLKVGTRCSPLALIQARLVERALAPYCARTDLVEVKTSGDIVSDVPLTEVGGKALFLK
CEEECCCCCCCHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCEECCCCHHHCCCCHHHHH
ELEEKLLTGEIDIAVHSMKDVPAFYHDDLEVVPVLKRAIPNDVFVSFRYPNMLSLPNGAV
HHHHHHHCCCCEEEEECHHCCCHHHCCCCHHHHHHHHHCCCCEEEEEECCCEEECCCCCE
IGTCAPRRVVQLNQRFRVIPLRGNIATRIEKAKNLDGIILAFCALERLGLRRMISEVIHE
EECCCCHHHHHHCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NVMLPGVAQGALCVEFRKKDRFLRELILNISDRETIICTAAERAFLEEINGDCKTALGAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHH
AIVKNNILYFTGMLAKDNRPRYFRTSGSCLNAKQVGRRAALALLRM
HHHHCCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSLKVGTRCSPLALIQARLVERALAPYCARTDLVEVKTSGDIVSDVPLTEVGGKALFLK
CEEECCCCCCCHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCEECCCCHHHCCCCHHHHH
ELEEKLLTGEIDIAVHSMKDVPAFYHDDLEVVPVLKRAIPNDVFVSFRYPNMLSLPNGAV
HHHHHHHCCCCEEEEECHHCCCHHHCCCCHHHHHHHHHCCCCEEEEEECCCEEECCCCCE
IGTCAPRRVVQLNQRFRVIPLRGNIATRIEKAKNLDGIILAFCALERLGLRRMISEVIHE
EECCCCHHHHHHCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NVMLPGVAQGALCVEFRKKDRFLRELILNISDRETIICTAAERAFLEEINGDCKTALGAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHH
AIVKNNILYFTGMLAKDNRPRYFRTSGSCLNAKQVGRRAALALLRM
HHHHCCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA