Click here to switch to the map view.

The map label for this gene is ispG

Identifier: 88608788

GI number: 88608788

Start: 710334

End: 711578

Strand: Reverse

Name: ispG

Synonym: NSE_0799

Alternate gene names: 88608788

Gene position: 711578-710334 (Counterclockwise)

Preceding gene: 88608685

Following gene: 88608588

Centisome position: 82.84

GC content: 41.61

Gene sequence:

>1245_bases
ATGTCTGAGCTCGATAGGAAGTCGCTTGTTGTTGATGTAGCGGGCGTTAGGATAGGGGGGGGAAACCCGATAGTTGTGCA
GTCCATGACCTCTGGCAGCAGAACAAATCCAAATGATGTATCAGCAATAGCTGTAGATGAATCCCGCGAGGCTATAGCTT
TGGCTAAGGCCGGCTCTGAGCTAATAAGGATTGCCATAAATAGTGAGAAGGCTGCAGCGGCGATTCCTTATATAAGGGAA
AATCTAGATAAATCCGGCTTTGAAAAAGTTCCGCTTGTTGGATGTGGTCAGTATGAGATTAAGCGCGTACTTGAAACACA
GACAGAAGCTATCAGATCCTTGGGTAAGATAAGAATTAATCCAGGTAATATAGGATTTGGTTCAAAAAGAGATACAAATT
TTGACAAAGTAATTGAGTTGATTTGCAAACATGATCTCCCCATTAGAATTGGCGTTAACTGGGGAAGTCTAGATCAATCG
GTGCTGCGTCAAATAATGGATGATAATGCAAAAAATGAGAAACCCAGCTCCTATAATGGAGTACTGAGATGTGCATTGAT
TCAGTCTGCTTTGGCTAGTGCGAAGCGTGCTGAGGAAGTTGGACTATCACCAGATAAAATTATACTTTCGTGTAAGGTAA
GCAGTTTTCAGGATTTAGTAGCGGTTTACTCCTCCTTGGCCGAACAATGTCGTTATCCACTGCACTTGGGTTTGACAGAA
GCAGGTATGGGTACTTCAGGAATAATAAAAACTACTGCAGCACTCTCAGTACTCCTTAGTAGGGGTATTGGTGATACAAT
ACGTGCTTCTTTAACACAGAAACCTGGTGAATCAAGGGTAATAGAAGTAGAAACCTGTCAGTTGATATTGCAGTCAATGG
GTTTAAGAGTCTTTGTTCCTCAGGTCACTTCATGTCCTGGTTGTGGAAGGACGAATGGAAATTATTTCCAGCAGATTAGC
AGTGACCTGAATGACTTCATAAAGGATAATCTAGTCGATTGGAAGAGGCTTTATCTAGGTGTTGAGAATTTCAAACTTGC
TGTAATGGGTTGTATTGTTAATGGTCCGGGTGAGAGTAAACATGCAGACGTGGGTATTAGCTTGCCTGGTTATAATGAAA
ATATGGTAGCGGCGGTTTTTATTGACGGAAAACCTTCAGCAAAACTTATTGGCGAAAACATTCTCGAGGAATCAAAGCGG
ATCATTTTAGAGTACATCAAGAACAAATATGCTCCACGAAGTTAA

Upstream 100 bases:

>100_bases
CCTGGATCTATGTGAAAAATTCAGGCAATTATTAGGTGCGACTCAGTAAGGGCCGACCTGATTTTCAGGTGAAGCTATAA
TCAGTAAGTTTGAGATTGTG

Downstream 100 bases:

>100_bases
GTGAAGGACCTCGTTGGGCATCAAAGCGGACTATCTTAGAATACATTAAGACTAGTGCGTTTGATAAAGTGAGACCGGAT
CTACTTTTACGGAAGTCATG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSELDRKSLVVDVAGVRIGGGNPIVVQSMTSGSRTNPNDVSAIAVDESREAIALAKAGSELIRIAINSEKAAAAIPYIRE
NLDKSGFEKVPLVGCGQYEIKRVLETQTEAIRSLGKIRINPGNIGFGSKRDTNFDKVIELICKHDLPIRIGVNWGSLDQS
VLRQIMDDNAKNEKPSSYNGVLRCALIQSALASAKRAEEVGLSPDKIILSCKVSSFQDLVAVYSSLAEQCRYPLHLGLTE
AGMGTSGIIKTTAALSVLLSRGIGDTIRASLTQKPGESRVIEVETCQLILQSMGLRVFVPQVTSCPGCGRTNGNYFQQIS
SDLNDFIKDNLVDWKRLYLGVENFKLAVMGCIVNGPGESKHADVGISLPGYNENMVAAVFIDGKPSAKLIGENILEESKR
IILEYIKNKYAPRS

Sequences:

>Translated_414_residues
MSELDRKSLVVDVAGVRIGGGNPIVVQSMTSGSRTNPNDVSAIAVDESREAIALAKAGSELIRIAINSEKAAAAIPYIRE
NLDKSGFEKVPLVGCGQYEIKRVLETQTEAIRSLGKIRINPGNIGFGSKRDTNFDKVIELICKHDLPIRIGVNWGSLDQS
VLRQIMDDNAKNEKPSSYNGVLRCALIQSALASAKRAEEVGLSPDKIILSCKVSSFQDLVAVYSSLAEQCRYPLHLGLTE
AGMGTSGIIKTTAALSVLLSRGIGDTIRASLTQKPGESRVIEVETCQLILQSMGLRVFVPQVTSCPGCGRTNGNYFQQIS
SDLNDFIKDNLVDWKRLYLGVENFKLAVMGCIVNGPGESKHADVGISLPGYNENMVAAVFIDGKPSAKLIGENILEESKR
IILEYIKNKYAPRS
>Mature_413_residues
SELDRKSLVVDVAGVRIGGGNPIVVQSMTSGSRTNPNDVSAIAVDESREAIALAKAGSELIRIAINSEKAAAAIPYIREN
LDKSGFEKVPLVGCGQYEIKRVLETQTEAIRSLGKIRINPGNIGFGSKRDTNFDKVIELICKHDLPIRIGVNWGSLDQSV
LRQIMDDNAKNEKPSSYNGVLRCALIQSALASAKRAEEVGLSPDKIILSCKVSSFQDLVAVYSSLAEQCRYPLHLGLTEA
GMGTSGIIKTTAALSVLLSRGIGDTIRASLTQKPGESRVIEVETCQLILQSMGLRVFVPQVTSCPGCGRTNGNYFQQISS
DLNDFIKDNLVDWKRLYLGVENFKLAVMGCIVNGPGESKHADVGISLPGYNENMVAAVFIDGKPSAKLIGENILEESKRI
ILEYIKNKYAPRS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=392, Percent_Identity=30.3571428571429, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44801; Mature: 44670

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELDRKSLVVDVAGVRIGGGNPIVVQSMTSGSRTNPNDVSAIAVDESREAIALAKAGSE
CCCCCCHHEEEEEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEEHHHCCCE
LIRIAINSEKAAAAIPYIRENLDKSGFEKVPLVGCGQYEIKRVLETQTEAIRSLGKIRIN
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEC
PGNIGFGSKRDTNFDKVIELICKHDLPIRIGVNWGSLDQSVLRQIMDDNAKNEKPSSYNG
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHH
VLRCALIQSALASAKRAEEVGLSPDKIILSCKVSSFQDLVAVYSSLAEQCRYPLHLGLTE
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCEEECCHH
AGMGTSGIIKTTAALSVLLSRGIGDTIRASLTQKPGESRVIEVETCQLILQSMGLRVFVP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCEEEEC
QVTSCPGCGRTNGNYFQQISSDLNDFIKDNLVDWKRLYLGVENFKLAVMGCIVNGPGESK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
HADVGISLPGYNENMVAAVFIDGKPSAKLIGENILEESKRIILEYIKNKYAPRS
CCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SELDRKSLVVDVAGVRIGGGNPIVVQSMTSGSRTNPNDVSAIAVDESREAIALAKAGSE
CCCCCHHEEEEEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEEHHHCCCE
LIRIAINSEKAAAAIPYIRENLDKSGFEKVPLVGCGQYEIKRVLETQTEAIRSLGKIRIN
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEC
PGNIGFGSKRDTNFDKVIELICKHDLPIRIGVNWGSLDQSVLRQIMDDNAKNEKPSSYNG
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHH
VLRCALIQSALASAKRAEEVGLSPDKIILSCKVSSFQDLVAVYSSLAEQCRYPLHLGLTE
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCEEECCHH
AGMGTSGIIKTTAALSVLLSRGIGDTIRASLTQKPGESRVIEVETCQLILQSMGLRVFVP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCEEEEC
QVTSCPGCGRTNGNYFQQISSDLNDFIKDNLVDWKRLYLGVENFKLAVMGCIVNGPGESK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
HADVGISLPGYNENMVAAVFIDGKPSAKLIGENILEESKRIILEYIKNKYAPRS
CCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA