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The map label for this gene is rpoA

Identifier: 88608775

GI number: 88608775

Start: 235540

End: 236541

Strand: Direct

Name: rpoA

Synonym: NSE_0289

Alternate gene names: 88608775

Gene position: 235540-236541 (Clockwise)

Preceding gene: 88608270

Following gene: 88608662

Centisome position: 27.42

GC content: 42.81

Gene sequence:

>1002_bases
ATGAGTGCTGTCTTAGATAAGGGTTCTTTAGTCGATGTTTTTTCTTCTCCTTCTGTTGTTTTTAACCAAATCCGAGAAGG
GTACTGTGCCGAGTTCATTGTTGAGCCCTTGCGTGTGGGCTTTGGGCTGACAATTGGGAATGCGATGCGTAGGGTTCTCC
TTTCATCCCTTAGTGGTTTTGCGATAAGTGCTGTTGGGATTAAGGGTTTAACTCACGAGTTTTCTTGTATACCTGGAGTG
CGTGAGGATTTTGCTGACCTTGCGCTCAACTTAAAAAAAGTGGTTTTAAAGAGCATTTCTGGTGCTACATGCGGAAATTT
GCATCTTTCAGTTACGGATGGAGGTGCTGTGTTCTCTAATATGATTTCTCCTAGTCACGACTTCGAAGTTGTTAATGGGG
ATTTGCTGATATGTAATGTTGCTGAGGGGGTTTCATTAGAGATGGAAATGAAGGTGAGTAGCGGTTTCGGATATGTGTCG
TCTGTCTCTGTCAGGAAAGATGAATATGACCTTGAAGGTGCTGTCCCAATAGACGCAATTTATAATCCTGTTCGTGCTGT
TAATTTTACAGTCAAGCCTACTAGTGCTGGCAGCTTTGCTGGGCACGACAAGTTGATTCTTTATGTTGAAACAAATGGTG
CCATGGATCCAAAGACTGCTGTTCTTGAGGCAAGTAAGATATTGTCGACTCAGGCAAGGTGTTTTCTTAATATTGCGGAC
CCCGAACATCGAGTTCATGGAGTTCCCTGTGGTGTTTCCACTTCTGACAGGAATGATGCCAGTGATCTTTTGTCTGCGCG
CATCGATCGTTTATATTTGTCTGCAAGAGCAAGGAAATGTCTCAATGGAGAGAATATAGTTTACATAAGGGATTTGGTTT
CTAGAACAGAGGCTGATTTGTTGAAAGCACCAAACTTCGGTAGGCGCTCTTTAGAAGAAGTAAAAAAAGAGCTGTTTAGC
AAGGGATTGAGTCTTGGTATGAATCTCGATAGTCATGGTTGA

Upstream 100 bases:

>100_bases
CTTCAGGACATGACGCCTGTTCCACATAATGGAGTAAGGTTGAGAAGAAGGCGTAGGGTCTAGTCTTTTATATATGATTT
TTGACGTTGTGTAGTTTATT

Downstream 100 bases:

>100_bases
ACTTTATGGGAATTTATTATGAAGCACAGGGTAAAGAAGCATAGATTATCAAGGAATTCCTCACATAGGATGTCACTTAT
CTGCAATCTTTCCGTCTCAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSAVLDKGSLVDVFSSPSVVFNQIREGYCAEFIVEPLRVGFGLTIGNAMRRVLLSSLSGFAISAVGIKGLTHEFSCIPGV
REDFADLALNLKKVVLKSISGATCGNLHLSVTDGGAVFSNMISPSHDFEVVNGDLLICNVAEGVSLEMEMKVSSGFGYVS
SVSVRKDEYDLEGAVPIDAIYNPVRAVNFTVKPTSAGSFAGHDKLILYVETNGAMDPKTAVLEASKILSTQARCFLNIAD
PEHRVHGVPCGVSTSDRNDASDLLSARIDRLYLSARARKCLNGENIVYIRDLVSRTEADLLKAPNFGRRSLEEVKKELFS
KGLSLGMNLDSHG

Sequences:

>Translated_333_residues
MSAVLDKGSLVDVFSSPSVVFNQIREGYCAEFIVEPLRVGFGLTIGNAMRRVLLSSLSGFAISAVGIKGLTHEFSCIPGV
REDFADLALNLKKVVLKSISGATCGNLHLSVTDGGAVFSNMISPSHDFEVVNGDLLICNVAEGVSLEMEMKVSSGFGYVS
SVSVRKDEYDLEGAVPIDAIYNPVRAVNFTVKPTSAGSFAGHDKLILYVETNGAMDPKTAVLEASKILSTQARCFLNIAD
PEHRVHGVPCGVSTSDRNDASDLLSARIDRLYLSARARKCLNGENIVYIRDLVSRTEADLLKAPNFGRRSLEEVKKELFS
KGLSLGMNLDSHG
>Mature_332_residues
SAVLDKGSLVDVFSSPSVVFNQIREGYCAEFIVEPLRVGFGLTIGNAMRRVLLSSLSGFAISAVGIKGLTHEFSCIPGVR
EDFADLALNLKKVVLKSISGATCGNLHLSVTDGGAVFSNMISPSHDFEVVNGDLLICNVAEGVSLEMEMKVSSGFGYVSS
VSVRKDEYDLEGAVPIDAIYNPVRAVNFTVKPTSAGSFAGHDKLILYVETNGAMDPKTAVLEASKILSTQARCFLNIADP
EHRVHGVPCGVSTSDRNDASDLLSARIDRLYLSARARKCLNGENIVYIRDLVSRTEADLLKAPNFGRRSLEEVKKELFSK
GLSLGMNLDSHG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=332, Percent_Identity=36.144578313253, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_NEOSM (Q2GEB6)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506180.1
- ProteinModelPortal:   Q2GEB6
- STRING:   Q2GEB6
- GeneID:   3931778
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0289
- TIGR:   NSE_0289
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   NSEN222891:NSE_0289-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35796; Mature: 35665

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVLDKGSLVDVFSSPSVVFNQIREGYCAEFIVEPLRVGFGLTIGNAMRRVLLSSLSGF
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AISAVGIKGLTHEFSCIPGVREDFADLALNLKKVVLKSISGATCGNLHLSVTDGGAVFSN
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHH
MISPSHDFEVVNGDLLICNVAEGVSLEMEMKVSSGFGYVSSVSVRKDEYDLEGAVPIDAI
HCCCCCCCEEECCCEEEEEECCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHH
YNPVRAVNFTVKPTSAGSFAGHDKLILYVETNGAMDPKTAVLEASKILSTQARCFLNIAD
HCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECC
PEHRVHGVPCGVSTSDRNDASDLLSARIDRLYLSARARKCLNGENIVYIRDLVSRTEADL
CCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
LKAPNFGRRSLEEVKKELFSKGLSLGMNLDSHG
HCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCC
>Mature Secondary Structure 
SAVLDKGSLVDVFSSPSVVFNQIREGYCAEFIVEPLRVGFGLTIGNAMRRVLLSSLSGF
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AISAVGIKGLTHEFSCIPGVREDFADLALNLKKVVLKSISGATCGNLHLSVTDGGAVFSN
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHH
MISPSHDFEVVNGDLLICNVAEGVSLEMEMKVSSGFGYVSSVSVRKDEYDLEGAVPIDAI
HCCCCCCCEEECCCEEEEEECCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHH
YNPVRAVNFTVKPTSAGSFAGHDKLILYVETNGAMDPKTAVLEASKILSTQARCFLNIAD
HCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECC
PEHRVHGVPCGVSTSDRNDASDLLSARIDRLYLSARARKCLNGENIVYIRDLVSRTEADL
CCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
LKAPNFGRRSLEEVKKELFSKGLSLGMNLDSHG
HCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA