Click here to switch to the map view.

The map label for this gene is fabI

Identifier: 88608759

GI number: 88608759

Start: 34305

End: 35102

Strand: Direct

Name: fabI

Synonym: NSE_0043

Alternate gene names: 88608759

Gene position: 34305-35102 (Clockwise)

Preceding gene: 88608305

Following gene: 88608119

Centisome position: 3.99

GC content: 40.1

Gene sequence:

>798_bases
ATGCTGAAGGGAAAGTTGGGTGTAATATTGGGTGTTGCGAATGACCGTTCGATTGCATATGCAATAGGAAAGATTTGTAG
AGAAAATGGGGCGGAGCTGATTCTTACATGTCCGACTGACCAATTTAAAAAACGTTTAGGTCCAATAGCTGAGGAATTTG
GAGGTTCCGCACTTGTGTGTTGTGATGTTTCACAGGAAAGCCAGGTGGAAGAACTCTTTCGCTTTATTAAGGAAAAATAT
GGAAGGCTCGACTTTGTCGTACACTCCGTTGCTTTTTCGGATAAGGAAGAACTACAGGGTGATTATATAAATACTTCTCT
TAGTAATTTTTTGAATTCAATGCATATTTCATGCTATTCCCTCACTTCGGTTTGTAGATATGCTTTGCCATTGATGAAGG
AAAGTGGTGGCAGTGTTCTTACTTTGTCCTACTACGGAGCTGAAAAAGTGATTCCTCACTATAATGTTATGGGTGTGTGT
AAAGCTGCTTTAGAAGCAAGTGTCAAATATTTAGCTGTTGATCTTGGTAAATACAATATAAGGATTAATACAATTTCTGC
AGGACCGATTAAGACTCTTGCTTCCTCTGCAATTGGAAAATTTTCCTACATACTTAAGTGGAACAAGCATAATGCGCCTA
TAAAGAGGAATACCACTAAGGAAGACGTTGCTGGTGCAGCCCTCTACTTTTTGAGTGATCTAAGCGGTGGAACCACAGGT
AGTACATTACACGTTGACTGTGGATATCATGTGATTGGTATGAGAGCCGTGGAGCTACCATCGGATATCATGACCTAA

Upstream 100 bases:

>100_bases
GAAAATACATTTTACTCCTTCTTGGAGTTGCTAATCATTAAGTTCTATTTCTTAGTTTATCATAGTACAATACTGTGGTT
GTCTTAGATGTGGTGGGTTA

Downstream 100 bases:

>100_bases
TGATCAATTTGTATCTGATCTATCTAATTAGCGTATAGGGATCCTGATTAAAAACTTTCACCCACCTCCGACAAACTCTA
CGATTTCTACTGAATCACCT

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLKGKLGVILGVANDRSIAYAIGKICRENGAELILTCPTDQFKKRLGPIAEEFGGSALVCCDVSQESQVEELFRFIKEKY
GRLDFVVHSVAFSDKEELQGDYINTSLSNFLNSMHISCYSLTSVCRYALPLMKESGGSVLTLSYYGAEKVIPHYNVMGVC
KAALEASVKYLAVDLGKYNIRINTISAGPIKTLASSAIGKFSYILKWNKHNAPIKRNTTKEDVAGAALYFLSDLSGGTTG
STLHVDCGYHVIGMRAVELPSDIMT

Sequences:

>Translated_265_residues
MLKGKLGVILGVANDRSIAYAIGKICRENGAELILTCPTDQFKKRLGPIAEEFGGSALVCCDVSQESQVEELFRFIKEKY
GRLDFVVHSVAFSDKEELQGDYINTSLSNFLNSMHISCYSLTSVCRYALPLMKESGGSVLTLSYYGAEKVIPHYNVMGVC
KAALEASVKYLAVDLGKYNIRINTISAGPIKTLASSAIGKFSYILKWNKHNAPIKRNTTKEDVAGAALYFLSDLSGGTTG
STLHVDCGYHVIGMRAVELPSDIMT
>Mature_265_residues
MLKGKLGVILGVANDRSIAYAIGKICRENGAELILTCPTDQFKKRLGPIAEEFGGSALVCCDVSQESQVEELFRFIKEKY
GRLDFVVHSVAFSDKEELQGDYINTSLSNFLNSMHISCYSLTSVCRYALPLMKESGGSVLTLSYYGAEKVIPHYNVMGVC
KAALEASVKYLAVDLGKYNIRINTISAGPIKTLASSAIGKFSYILKWNKHNAPIKRNTTKEDVAGAALYFLSDLSGGTTG
STLHVDCGYHVIGMRAVELPSDIMT

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=25.1082251082251, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=39.7683397683398, Blast_Score=197, Evalue=4e-52,
Organism=Escherichia coli, GI1787905, Length=200, Percent_Identity=28, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=25.4826254826255, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=273, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=196, Percent_Identity=23.9795918367347, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=273, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357041, Length=221, Percent_Identity=26.2443438914027, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644337, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571526, Length=199, Percent_Identity=24.6231155778894, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28875; Mature: 28875

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGKLGVILGVANDRSIAYAIGKICRENGAELILTCPTDQFKKRLGPIAEEFGGSALVC
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCHHHHHHCCCEEEE
CDVSQESQVEELFRFIKEKYGRLDFVVHSVAFSDKEELQGDYINTSLSNFLNSMHISCYS
EECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
LTSVCRYALPLMKESGGSVLTLSYYGAEKVIPHYNVMGVCKAALEASVKYLAVDLGKYNI
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECEEEE
RINTISAGPIKTLASSAIGKFSYILKWNKHNAPIKRNTTKEDVAGAALYFLSDLSGGTTG
EEEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
STLHVDCGYHVIGMRAVELPSDIMT
CEEEEECCEEEEEEEEEECCHHHCC
>Mature Secondary Structure
MLKGKLGVILGVANDRSIAYAIGKICRENGAELILTCPTDQFKKRLGPIAEEFGGSALVC
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCHHHHHHCCCEEEE
CDVSQESQVEELFRFIKEKYGRLDFVVHSVAFSDKEELQGDYINTSLSNFLNSMHISCYS
EECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
LTSVCRYALPLMKESGGSVLTLSYYGAEKVIPHYNVMGVCKAALEASVKYLAVDLGKYNI
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECEEEE
RINTISAGPIKTLASSAIGKFSYILKWNKHNAPIKRNTTKEDVAGAALYFLSDLSGGTTG
EEEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
STLHVDCGYHVIGMRAVELPSDIMT
CEEEEECCEEEEEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]