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The map label for this gene is lepB

Identifier: 88608750

GI number: 88608750

Start: 696464

End: 697222

Strand: Direct

Name: lepB

Synonym: NSE_0784

Alternate gene names: 88608750

Gene position: 696464-697222 (Clockwise)

Preceding gene: 88608472

Following gene: 88608188

Centisome position: 81.08

GC content: 43.08

Gene sequence:

>759_bases
ATGGCACATTCATCAAGAATCTTACACACACTCAGGAATGCTTGTAGGAGTAAAACGGTCTGGTTGATAATCGGTCTTGT
AGCTACGCGTAGTCTGTTTTACGAGCCGTTCATCATACCTTCGGGCTCGATGAAGAAGACGCTTCTGGCTGGGGACTATA
TAGTGGCTTCTAAGTATGCGTATGGATATAGCAAGTACTCGTTTCCCTTCTCTCCGTCTTTTATAAAGGGAGACCCAAGA
ATTCTATATAAACCGCCAAAACGCGGAGACGTAGTCATATTCAGAAACCCGCACAAGGACAACACAAATTATGTCAAAAG
AGTCATTGGACTACCAGGAGATAGAATCCAACTGATTGGTAGCAGAGTTTATGTGAATCACAAGCCTATAAAAAGAGTTG
AAGACGGAATTTTTATGGACTCTAGCACACCAGAAGTACAAAGTTTCACAGAAATTCTTGATACCGGGCTTGCATACCCA
GTGCTACAGAAAACAACTGATGGACCAGCAAACAACACCGGTGTGTACTATGTGCCACAAGGACATTTTTTCGTTCTTGG
TGATAATAGAGATGATTCTACAGATAGTAGATTCCTCGCAGCTGTAGGCTTCATTCCAGCAGAGTATTTGGTCGGCAGAG
CAGAGCGTGTTCTGTTTTCGTTCCGTATAGGAGAGAGCAAATTCATACCGATAAAACTACGCTTGGAAAGAACATGGAAA
TCGCTTCGTCCAGAGGAGCTCGGCCAAAAAAAGAGATAA

Upstream 100 bases:

>100_bases
TCTCCTGACATGAAACATGCTGTCACTGTTCAGGGTGGAGTTGAATTGACTAGGGCATAAGTATTCTGCTATCAGTAGAT
GAGGCTCCGTTTCCATATAA

Downstream 100 bases:

>100_bases
AACTACCTAGGAAGGCATGACCAGATCGAGTAGTAAGTTCTTGGAACCCTACCTTTCCTATACTGGAAGCGAAAGCGTTA
TATCATCTATTAGGCCAGAA

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MAHSSRILHTLRNACRSKTVWLIIGLVATRSLFYEPFIIPSGSMKKTLLAGDYIVASKYAYGYSKYSFPFSPSFIKGDPR
ILYKPPKRGDVVIFRNPHKDNTNYVKRVIGLPGDRIQLIGSRVYVNHKPIKRVEDGIFMDSSTPEVQSFTEILDTGLAYP
VLQKTTDGPANNTGVYYVPQGHFFVLGDNRDDSTDSRFLAAVGFIPAEYLVGRAERVLFSFRIGESKFIPIKLRLERTWK
SLRPEELGQKKR

Sequences:

>Translated_252_residues
MAHSSRILHTLRNACRSKTVWLIIGLVATRSLFYEPFIIPSGSMKKTLLAGDYIVASKYAYGYSKYSFPFSPSFIKGDPR
ILYKPPKRGDVVIFRNPHKDNTNYVKRVIGLPGDRIQLIGSRVYVNHKPIKRVEDGIFMDSSTPEVQSFTEILDTGLAYP
VLQKTTDGPANNTGVYYVPQGHFFVLGDNRDDSTDSRFLAAVGFIPAEYLVGRAERVLFSFRIGESKFIPIKLRLERTWK
SLRPEELGQKKR
>Mature_251_residues
AHSSRILHTLRNACRSKTVWLIIGLVATRSLFYEPFIIPSGSMKKTLLAGDYIVASKYAYGYSKYSFPFSPSFIKGDPRI
LYKPPKRGDVVIFRNPHKDNTNYVKRVIGLPGDRIQLIGSRVYVNHKPIKRVEDGIFMDSSTPEVQSFTEILDTGLAYPV
LQKTTDGPANNTGVYYVPQGHFFVLGDNRDDSTDSRFLAAVGFIPAEYLVGRAERVLFSFRIGESKFIPIKLRLERTWKS
LRPEELGQKKR

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=258, Percent_Identity=32.1705426356589, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 28628; Mature: 28497

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSSRILHTLRNACRSKTVWLIIGLVATRSLFYEPFIIPSGSMKKTLLAGDYIVASKYA
CCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCEEECCCCCCCEEEECCEEEEEEEE
YGYSKYSFPFSPSFIKGDPRILYKPPKRGDVVIFRNPHKDNTNYVKRVIGLPGDRIQLIG
CCCCEECCCCCCCEECCCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEE
SRVYVNHKPIKRVEDGIFMDSSTPEVQSFTEILDTGLAYPVLQKTTDGPANNTGVYYVPQ
EEEEECCCCHHHHHCCEEECCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEC
GHFFVLGDNRDDSTDSRFLAAVGFIPAEYLVGRAERVLFSFRIGESKFIPIKLRLERTWK
CEEEEEECCCCCCCCHHEEHHHHCCCHHHHHHHHHEEEEEEEECCCEEEEEEEEEEHHHH
SLRPEELGQKKR
HCCHHHHCCCCC
>Mature Secondary Structure 
AHSSRILHTLRNACRSKTVWLIIGLVATRSLFYEPFIIPSGSMKKTLLAGDYIVASKYA
CCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCEEECCCCCCCEEEECCEEEEEEEE
YGYSKYSFPFSPSFIKGDPRILYKPPKRGDVVIFRNPHKDNTNYVKRVIGLPGDRIQLIG
CCCCEECCCCCCCEECCCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEE
SRVYVNHKPIKRVEDGIFMDSSTPEVQSFTEILDTGLAYPVLQKTTDGPANNTGVYYVPQ
EEEEECCCCHHHHHCCEEECCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEC
GHFFVLGDNRDDSTDSRFLAAVGFIPAEYLVGRAERVLFSFRIGESKFIPIKLRLERTWK
CEEEEEECCCCCCCCHHEEHHHHCCCHHHHHHHHHEEEEEEEECCCEEEEEEEEEEHHHH
SLRPEELGQKKR
HCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA