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The map label for this gene is bioF

Identifier: 88608742

GI number: 88608742

Start: 536418

End: 537599

Strand: Direct

Name: bioF

Synonym: NSE_0613

Alternate gene names: 88608742

Gene position: 536418-537599 (Clockwise)

Preceding gene: 88608683

Following gene: 88608178

Centisome position: 62.45

GC content: 41.12

Gene sequence:

>1182_bases
ATGGAAGAAGCTTCGGAAAATACATCTTGCTATGCAGATTTTCTCACACAAAGAAGCGCAAAGAATCTCTATCGTCAACT
CGAGACGTGCCTGAATGAAAGAAGTTGCATTAATTTCTCGTCAAATGATTACCTTGGCTTAAGTAAATCTAGGGAGACAT
TAGAAGCTGCCTATTCATATGGTTTAAAGAGCGGGATTGGGGCGACTGGATCGAGACTTCTCTCAGGAAACAACACAATT
TTTAAAGAACTCGAACAACAAATCGCAAAAGACAAAAATACCGAAGCTGCCTTAGTAATGGGTTCGGGTTTTCAGACAAA
CATAACGGTACTTTCCACTCTTCTTGATAGATCTGTCCTTGGCCAAAGAGCAATTGTGTTCTTCGATAGGTTGAATCACT
CCAGCCTGTACCACGCAATACTTCTTGCTGGTGCAGAAATGGTCCGTTATAGACACTGTGATATGGAAGATCTTTCCCGA
TTAATGGAAAAATACTCTGCGGATAGACGTCCAAAATTCATAGTAACGGAGACATTATTCGGAATGGATGGCGATGTACA
AGACATGGAGCGTATAGCTACTTTAGCACATCAACATAAGGCAGTGCTGTATCTGGATGAAGCACATGCTACAGGCATTC
TTGGAAAGAGTGGGTATGGTCTCTCAACTAACTTTGACCTTTCAGGGATCACACATGTAATCATGGGAACATTTAGCAAG
GCAATTGGGGTCTTTGGAAGCTATATCGCATCTTCTACATTGATAAAAAATTACCTGATCAACAAATGCCCTGGTTTGAT
ATACTCGACCGCACTCCCACCTATTCTGCTAGGTGCAATAGCAAAAGCATGGGAAACGGTAGCGCTCTTAAGCAAAGAAA
GAAAAACACTGCTAGAAATGGCAAGTAAAGCAAGAAAATTTCTTAGCCAGGAAGGGTACAATATCGGCAATTCAACCAAT
CATATAATTCCCATCATACTTGGAGAGGAAGAAAAAGTTCTCACATTGAAGCAAAAACTTCTCGGAAAAGGAATAATTGT
CTCTGCTGTGCGTCCACCAACTGTACCTGTGAACACATCAAGAATTCGTATAGCATTGTGTGCATTTCACACTAAACACG
ACATTGCCCACCTTCTACAAAACATCAAAAGCACCAAAAACCTAACCGAAGACACTGCATGA

Upstream 100 bases:

>100_bases
AGTCGCGTTGCCTCATACGAGCGTGAATAGAAGTAACATTTTTTAAGAAACAACTATAAAATCAAGATCATCTGGATCAC
TTAAAAATCTCTGATTCTTT

Downstream 100 bases:

>100_bases
ATAGGAAAGTGATACTCTTATGTCATGGTTGGGGTTTCGATCAAAAATTCTGGTCACCGATAATACAACTTTTTGCAAAA
GATGATGTCAAACTACTTGA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MEEASENTSCYADFLTQRSAKNLYRQLETCLNERSCINFSSNDYLGLSKSRETLEAAYSYGLKSGIGATGSRLLSGNNTI
FKELEQQIAKDKNTEAALVMGSGFQTNITVLSTLLDRSVLGQRAIVFFDRLNHSSLYHAILLAGAEMVRYRHCDMEDLSR
LMEKYSADRRPKFIVTETLFGMDGDVQDMERIATLAHQHKAVLYLDEAHATGILGKSGYGLSTNFDLSGITHVIMGTFSK
AIGVFGSYIASSTLIKNYLINKCPGLIYSTALPPILLGAIAKAWETVALLSKERKTLLEMASKARKFLSQEGYNIGNSTN
HIIPIILGEEEKVLTLKQKLLGKGIIVSAVRPPTVPVNTSRIRIALCAFHTKHDIAHLLQNIKSTKNLTEDTA

Sequences:

>Translated_393_residues
MEEASENTSCYADFLTQRSAKNLYRQLETCLNERSCINFSSNDYLGLSKSRETLEAAYSYGLKSGIGATGSRLLSGNNTI
FKELEQQIAKDKNTEAALVMGSGFQTNITVLSTLLDRSVLGQRAIVFFDRLNHSSLYHAILLAGAEMVRYRHCDMEDLSR
LMEKYSADRRPKFIVTETLFGMDGDVQDMERIATLAHQHKAVLYLDEAHATGILGKSGYGLSTNFDLSGITHVIMGTFSK
AIGVFGSYIASSTLIKNYLINKCPGLIYSTALPPILLGAIAKAWETVALLSKERKTLLEMASKARKFLSQEGYNIGNSTN
HIIPIILGEEEKVLTLKQKLLGKGIIVSAVRPPTVPVNTSRIRIALCAFHTKHDIAHLLQNIKSTKNLTEDTA
>Mature_393_residues
MEEASENTSCYADFLTQRSAKNLYRQLETCLNERSCINFSSNDYLGLSKSRETLEAAYSYGLKSGIGATGSRLLSGNNTI
FKELEQQIAKDKNTEAALVMGSGFQTNITVLSTLLDRSVLGQRAIVFFDRLNHSSLYHAILLAGAEMVRYRHCDMEDLSR
LMEKYSADRRPKFIVTETLFGMDGDVQDMERIATLAHQHKAVLYLDEAHATGILGKSGYGLSTNFDLSGITHVIMGTFSK
AIGVFGSYIASSTLIKNYLINKCPGLIYSTALPPILLGAIAKAWETVALLSKERKTLLEMASKARKFLSQEGYNIGNSTN
HIIPIILGEEEKVLTLKQKLLGKGIIVSAVRPPTVPVNTSRIRIALCAFHTKHDIAHLLQNIKSTKNLTEDTA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=343, Percent_Identity=30.9037900874636, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI284448556, Length=343, Percent_Identity=30.9037900874636, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI83977440, Length=353, Percent_Identity=32.8611898016997, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI83977444, Length=353, Percent_Identity=32.8611898016997, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI83977442, Length=353, Percent_Identity=32.8611898016997, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4502025, Length=350, Percent_Identity=30.8571428571429, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI40316939, Length=350, Percent_Identity=30.8571428571429, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI4758668, Length=374, Percent_Identity=29.9465240641711, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI119220554, Length=374, Percent_Identity=28.6096256684492, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI5454084, Length=257, Percent_Identity=25.6809338521401, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786993, Length=357, Percent_Identity=32.2128851540616, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1790046, Length=346, Percent_Identity=30.9248554913295, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994529, Length=353, Percent_Identity=33.4277620396601, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32566772, Length=371, Percent_Identity=29.1105121293801, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17560914, Length=363, Percent_Identity=25.6198347107438, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560912, Length=363, Percent_Identity=25.6198347107438, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71982625, Length=283, Percent_Identity=22.6148409893993, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71982617, Length=283, Percent_Identity=22.6148409893993, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320438, Length=369, Percent_Identity=30.8943089430894, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320267, Length=369, Percent_Identity=28.1842818428184, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323954, Length=229, Percent_Identity=26.6375545851528, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24662918, Length=345, Percent_Identity=29.2753623188406, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17137420, Length=367, Percent_Identity=30.2452316076294, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17136286, Length=374, Percent_Identity=28.6096256684492, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24653280, Length=381, Percent_Identity=25.4593175853018, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653276, Length=381, Percent_Identity=25.4593175853018, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653278, Length=381, Percent_Identity=25.4593175853018, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43366; Mature: 43366

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEASENTSCYADFLTQRSAKNLYRQLETCLNERSCINFSSNDYLGLSKSRETLEAAYSY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHH
GLKSGIGATGSRLLSGNNTIFKELEQQIAKDKNTEAALVMGSGFQTNITVLSTLLDRSVL
HHHCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
GQRAIVFFDRLNHSSLYHAILLAGAEMVRYRHCDMEDLSRLMEKYSADRRPKFIVTETLF
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEHHHH
GMDGDVQDMERIATLAHQHKAVLYLDEAHATGILGKSGYGLSTNFDLSGITHVIMGTFSK
CCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AIGVFGSYIASSTLIKNYLINKCPGLIYSTALPPILLGAIAKAWETVALLSKERKTLLEM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKARKFLSQEGYNIGNSTNHIIPIILGEEEKVLTLKQKLLGKGIIVSAVRPPTVPVNTS
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
RIRIALCAFHTKHDIAHLLQNIKSTKNLTEDTA
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEEASENTSCYADFLTQRSAKNLYRQLETCLNERSCINFSSNDYLGLSKSRETLEAAYSY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHH
GLKSGIGATGSRLLSGNNTIFKELEQQIAKDKNTEAALVMGSGFQTNITVLSTLLDRSVL
HHHCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
GQRAIVFFDRLNHSSLYHAILLAGAEMVRYRHCDMEDLSRLMEKYSADRRPKFIVTETLF
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEHHHH
GMDGDVQDMERIATLAHQHKAVLYLDEAHATGILGKSGYGLSTNFDLSGITHVIMGTFSK
CCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AIGVFGSYIASSTLIKNYLINKCPGLIYSTALPPILLGAIAKAWETVALLSKERKTLLEM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKARKFLSQEGYNIGNSTNHIIPIILGEEEKVLTLKQKLLGKGIIVSAVRPPTVPVNTS
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
RIRIALCAFHTKHDIAHLLQNIKSTKNLTEDTA
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA