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The map label for this gene is purH

Identifier: 88608740

GI number: 88608740

Start: 154794

End: 156266

Strand: Reverse

Name: purH

Synonym: NSE_0185

Alternate gene names: 88608740

Gene position: 156266-154794 (Counterclockwise)

Preceding gene: 88608203

Following gene: 88608824

Centisome position: 18.19

GC content: 40.46

Gene sequence:

>1473_bases
ATGATAAAAAGAGCGCTTATATCCGTTTACGATAAGACGGATCTCTTGCCTCTGGCACACAAATTACAAGCTGCAAATGT
TGAAATCATTGCAACTGGAAAGACACATAAATATCTCCTAGAGAATCAAATCAGAGCAATCAATTTATCTGATTATACGC
ACCAACCAGAAATACTAGGTGGTAGGGTGAAAACGCTGCATCCCCTAATACACGCAGGCTTGCTGGCAGATCCGGCTTTA
CACGAAGCTGAAATGCAAACACTGACAATAAAACAAATAGATCTGGTAGTTGTAAATCTTTACCCATTTGAAAAGTGCGT
AAATAGTGGTGCCCCAGAAAGTGAAATTATAGAAAACATAGATATTGGTGGTGTTTCACTATTAAGGTCTGCAGCTAAGA
ATTTTAAAAATGTTTGTGTCCTCTCTGACCCAAGCGATTACGGTACATTCGACGTAAACCCAACTTTATCTTTCAGAACT
AAAATGGCAAGAAAAGCATTTGCACGGGTCGCAAGATATGACTGTAAGATAGCAGAATGGTTCGCTTCCTCTACTTCTTT
GCCAGAGGTGCTAAATCTATCTGTCATGAAGAAAAATGATTTCAGATACGGTGAAAATCCCCATCAGAACGCATCTTTCT
ACTCTAATGGAGAGTTTCCACTCACAAAACTTCAGGGTAAAGAACTAAGTTATAACAACCTGCTCGACCTAGACAGCGCA
TTGTCGATTGTGACAAACTTCACTGAACCTACTTGTGCAATTATTAAACACAGTAATCCCTGCGGTGTAGCCTCACACAA
ATCCAGTCTGGAACAGGCATACGAGAAGGCACTCGCTGCAGATTCGTTAAGTGCTTTTGGTGGTGTAGTTGCATTGAATC
AAATTGTTACTAGGAACGTGGCAGAAAAGCTTTTGAATACCTTCTTTGAAGTCATCGTTGCTTATGGAATCACACAGGAC
GCTATATTTCTCTTTTCAAACAAACCAAATTTACGCATCCTTACATGCAAGGGTTATACTGCACCAGAAAAACACATGCT
CAGTTTGCTCGGGGGACTCTTAGTACAATCTGGAAATACAAAATTATTTCAGGACTTTGATATTGTCACAAAAAGACAAC
CAACAAATGAAGAAGTTAATCAACTTATTTTTGCCTGGAAGATCTGCAAATATGTCAAGTCAAACGCAATAGTGACTGCC
CACGATTACACAACTGTAGGAATAGGTGCCGGTCAAATGAGCCGCGTTAAGAGTGTAGAAATAGCACTGGGAAAAAGCAA
AGTCGGGCAGCCTCTTGCGATGGCGTCGGATGCGTTCTTTCCATTTGCGGATAGTATAGAGTTAGCAGCAAAGAGTGGTG
TAAAGTCAATTATCCAACCAGGTGGCTCAATCAGAGATAAAGAAGTAATAGAAGCAGCCGATCATCATAACATTGCGATG
ATATTTACGCGAATGAGGCATTTCAGACACTGA

Upstream 100 bases:

>100_bases
TTGCCTATTACTTGTGCTAAAAACAACCTAGTACTTATTTTCGATTTGTACTTAAGGTTTCGGCTCAGTACCATGTGTAT
TTTTGACTTGAAAAACTAAG

Downstream 100 bases:

>100_bases
TCGCAACCTTCTTGGATGGTACGGGACCTAGAAAATTTTTAGATATTTTCCTTGAACTTGACTTTTGTTGTTTTAAAGAA
TTCCCCTTTTAAAACAAAAT

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MIKRALISVYDKTDLLPLAHKLQAANVEIIATGKTHKYLLENQIRAINLSDYTHQPEILGGRVKTLHPLIHAGLLADPAL
HEAEMQTLTIKQIDLVVVNLYPFEKCVNSGAPESEIIENIDIGGVSLLRSAAKNFKNVCVLSDPSDYGTFDVNPTLSFRT
KMARKAFARVARYDCKIAEWFASSTSLPEVLNLSVMKKNDFRYGENPHQNASFYSNGEFPLTKLQGKELSYNNLLDLDSA
LSIVTNFTEPTCAIIKHSNPCGVASHKSSLEQAYEKALAADSLSAFGGVVALNQIVTRNVAEKLLNTFFEVIVAYGITQD
AIFLFSNKPNLRILTCKGYTAPEKHMLSLLGGLLVQSGNTKLFQDFDIVTKRQPTNEEVNQLIFAWKICKYVKSNAIVTA
HDYTTVGIGAGQMSRVKSVEIALGKSKVGQPLAMASDAFFPFADSIELAAKSGVKSIIQPGGSIRDKEVIEAADHHNIAM
IFTRMRHFRH

Sequences:

>Translated_490_residues
MIKRALISVYDKTDLLPLAHKLQAANVEIIATGKTHKYLLENQIRAINLSDYTHQPEILGGRVKTLHPLIHAGLLADPAL
HEAEMQTLTIKQIDLVVVNLYPFEKCVNSGAPESEIIENIDIGGVSLLRSAAKNFKNVCVLSDPSDYGTFDVNPTLSFRT
KMARKAFARVARYDCKIAEWFASSTSLPEVLNLSVMKKNDFRYGENPHQNASFYSNGEFPLTKLQGKELSYNNLLDLDSA
LSIVTNFTEPTCAIIKHSNPCGVASHKSSLEQAYEKALAADSLSAFGGVVALNQIVTRNVAEKLLNTFFEVIVAYGITQD
AIFLFSNKPNLRILTCKGYTAPEKHMLSLLGGLLVQSGNTKLFQDFDIVTKRQPTNEEVNQLIFAWKICKYVKSNAIVTA
HDYTTVGIGAGQMSRVKSVEIALGKSKVGQPLAMASDAFFPFADSIELAAKSGVKSIIQPGGSIRDKEVIEAADHHNIAM
IFTRMRHFRH
>Mature_490_residues
MIKRALISVYDKTDLLPLAHKLQAANVEIIATGKTHKYLLENQIRAINLSDYTHQPEILGGRVKTLHPLIHAGLLADPAL
HEAEMQTLTIKQIDLVVVNLYPFEKCVNSGAPESEIIENIDIGGVSLLRSAAKNFKNVCVLSDPSDYGTFDVNPTLSFRT
KMARKAFARVARYDCKIAEWFASSTSLPEVLNLSVMKKNDFRYGENPHQNASFYSNGEFPLTKLQGKELSYNNLLDLDSA
LSIVTNFTEPTCAIIKHSNPCGVASHKSSLEQAYEKALAADSLSAFGGVVALNQIVTRNVAEKLLNTFFEVIVAYGITQD
AIFLFSNKPNLRILTCKGYTAPEKHMLSLLGGLLVQSGNTKLFQDFDIVTKRQPTNEEVNQLIFAWKICKYVKSNAIVTA
HDYTTVGIGAGQMSRVKSVEIALGKSKVGQPLAMASDAFFPFADSIELAAKSGVKSIIQPGGSIRDKEVIEAADHHNIAM
IFTRMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=464, Percent_Identity=33.4051724137931, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=45.3333333333333, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71985564, Length=505, Percent_Identity=32.0792079207921, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71985574, Length=296, Percent_Identity=26.0135135135135, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323056, Length=469, Percent_Identity=34.5415778251599, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323768, Length=464, Percent_Identity=34.2672413793103, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24649832, Length=464, Percent_Identity=33.1896551724138, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54018; Mature: 54018

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRALISVYDKTDLLPLAHKLQAANVEIIATGKTHKYLLENQIRAINLSDYTHQPEILG
CCHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHCCCEEEECCCCCCCCHHCC
GRVKTLHPLIHAGLLADPALHEAEMQTLTIKQIDLVVVNLYPFEKCVNSGAPESEIIENI
CCHHHHHHHHHHHHHCCCHHHHHHHHEEEEEEEEEEEEEECCHHHHHCCCCCHHHHHHCC
DIGGVSLLRSAAKNFKNVCVLSDPSDYGTFDVNPTLSFRTKMARKAFARVARYDCKIAEW
CCCHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHH
FASSTSLPEVLNLSVMKKNDFRYGENPHQNASFYSNGEFPLTKLQGKELSYNNLLDLDSA
HHCCCCCCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHH
LSIVTNFTEPTCAIIKHSNPCGVASHKSSLEQAYEKALAADSLSAFGGVVALNQIVTRNV
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKLLNTFFEVIVAYGITQDAIFLFSNKPNLRILTCKGYTAPEKHMLSLLGGLLVQSGNT
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCC
KLFQDFDIVTKRQPTNEEVNQLIFAWKICKYVKSNAIVTAHDYTTVGIGAGQMSRVKSVE
CEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHEEE
IALGKSKVGQPLAMASDAFFPFADSIELAAKSGVKSIIQPGGSIRDKEVIEAADHHNIAM
EEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHH
IFTRMRHFRH
HHHHHHHHCC
>Mature Secondary Structure
MIKRALISVYDKTDLLPLAHKLQAANVEIIATGKTHKYLLENQIRAINLSDYTHQPEILG
CCHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHCCCEEEECCCCCCCCHHCC
GRVKTLHPLIHAGLLADPALHEAEMQTLTIKQIDLVVVNLYPFEKCVNSGAPESEIIENI
CCHHHHHHHHHHHHHCCCHHHHHHHHEEEEEEEEEEEEEECCHHHHHCCCCCHHHHHHCC
DIGGVSLLRSAAKNFKNVCVLSDPSDYGTFDVNPTLSFRTKMARKAFARVARYDCKIAEW
CCCHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHH
FASSTSLPEVLNLSVMKKNDFRYGENPHQNASFYSNGEFPLTKLQGKELSYNNLLDLDSA
HHCCCCCCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHH
LSIVTNFTEPTCAIIKHSNPCGVASHKSSLEQAYEKALAADSLSAFGGVVALNQIVTRNV
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKLLNTFFEVIVAYGITQDAIFLFSNKPNLRILTCKGYTAPEKHMLSLLGGLLVQSGNT
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCC
KLFQDFDIVTKRQPTNEEVNQLIFAWKICKYVKSNAIVTAHDYTTVGIGAGQMSRVKSVE
CEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHEEE
IALGKSKVGQPLAMASDAFFPFADSIELAAKSGVKSIIQPGGSIRDKEVIEAADHHNIAM
EEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHH
IFTRMRHFRH
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA