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The map label for this gene is ribF

Identifier: 88608739

GI number: 88608739

Start: 30303

End: 31214

Strand: Direct

Name: ribF

Synonym: NSE_0040

Alternate gene names: 88608739

Gene position: 30303-31214 (Clockwise)

Preceding gene: 88608206

Following gene: 88608305

Centisome position: 3.53

GC content: 41.89

Gene sequence:

>912_bases
ATGCAGGTTTACCATACTCCCGAGCTGCTTCCCCACCCTTCAGTCGTTACCTTTGGTAACTTTGACGGGGTACATTTAGG
GCATCTGGCCTTGCTCTCTGCGCTGTCCGGTGTCAGTAAGTTAGCTGATTTACATAGGGTTGTCATCACTTTTGATCCTC
ATCCATTCTGTTTTCTTAAGCGCAAAAAGGACTTCCTTATTACAGATCAGTACACTAAAACAGTTCTCCTTTCTCCATAT
GCGGATTCTCTATATTTCATAAAGTTCGATGATGAGCTTAGGAGTTTGTCTCCCGAGGATTTTCTGAAATTTCTGTCTTC
TTTTCTGAATCTCAAATACCTTATAAACGCCGAAAATTGCAGATTCGGAAAGTTTAGTTCCGGTGGCATAGCTGAAATAG
AAAAATATCAGGAATTGCTTTCATATAAGGCTGTAGTCATAAAGCCCGAGCTTAACGATTCTGGCAGTAGGTACTCCTCT
ACTCTGGTCCGGACAAGCATCAAGGATGGTGATATGCTGAGTGCAAGGCAACTCTTGGGGCGGTATCACAGTATACGTGG
CATTATTCGGAGAGATAGGGGCCTTGCTAGTACTATTGGTTTTCCCACAATCAATATTGCAATTCCCGAGGAGATAATTA
AGCCAAGACTTGGTGTGTACGTGGTTGATGTTTCCATAGAAGGTACTATACATAGGGGCATAGCAAATATTGGTAAAAGA
CCCACTACCTCTGGCAGTGGTGAAGTTATCTTAGAAGTGCATATTTTTAACTTCTCTGACAATGTATATGGCAAAGATGC
AACAGTGTATCTAAAGGCTTTTCTACGAGAAGAAAAGCTTTTTCCGTCTGTTGAGAGACTAAGAGAGCAAATCCTAGCAG
ATATCCGCGCTGTCCTTTCTATGAATTTCTAA

Upstream 100 bases:

>100_bases
CGATCTTGTTGAGGAGCGTGACTCGGAAGTTTTCTCTGATTCTGAGTTTTCCTTTGCTGAAGATGAGGGGGATGAGTAGG
TAGGGTTTTTGCCTCTGTAG

Downstream 100 bases:

>100_bases
GTTCATTTTCTCATTTGTGCAATCTCATTCTTCCTCAGTATGATACGTCTTTTTAGTTTAGTTGATACTAAAATTTACAT
ATAACAGTCAAGCTTATTTG

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQVYHTPELLPHPSVVTFGNFDGVHLGHLALLSALSGVSKLADLHRVVITFDPHPFCFLKRKKDFLITDQYTKTVLLSPY
ADSLYFIKFDDELRSLSPEDFLKFLSSFLNLKYLINAENCRFGKFSSGGIAEIEKYQELLSYKAVVIKPELNDSGSRYSS
TLVRTSIKDGDMLSARQLLGRYHSIRGIIRRDRGLASTIGFPTINIAIPEEIIKPRLGVYVVDVSIEGTIHRGIANIGKR
PTTSGSGEVILEVHIFNFSDNVYGKDATVYLKAFLREEKLFPSVERLREQILADIRAVLSMNF

Sequences:

>Translated_303_residues
MQVYHTPELLPHPSVVTFGNFDGVHLGHLALLSALSGVSKLADLHRVVITFDPHPFCFLKRKKDFLITDQYTKTVLLSPY
ADSLYFIKFDDELRSLSPEDFLKFLSSFLNLKYLINAENCRFGKFSSGGIAEIEKYQELLSYKAVVIKPELNDSGSRYSS
TLVRTSIKDGDMLSARQLLGRYHSIRGIIRRDRGLASTIGFPTINIAIPEEIIKPRLGVYVVDVSIEGTIHRGIANIGKR
PTTSGSGEVILEVHIFNFSDNVYGKDATVYLKAFLREEKLFPSVERLREQILADIRAVLSMNF
>Mature_303_residues
MQVYHTPELLPHPSVVTFGNFDGVHLGHLALLSALSGVSKLADLHRVVITFDPHPFCFLKRKKDFLITDQYTKTVLLSPY
ADSLYFIKFDDELRSLSPEDFLKFLSSFLNLKYLINAENCRFGKFSSGGIAEIEKYQELLSYKAVVIKPELNDSGSRYSS
TLVRTSIKDGDMLSARQLLGRYHSIRGIIRRDRGLASTIGFPTINIAIPEEIIKPRLGVYVVDVSIEGTIHRGIANIGKR
PTTSGSGEVILEVHIFNFSDNVYGKDATVYLKAFLREEKLFPSVERLREQILADIRAVLSMNF

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=285, Percent_Identity=32.6315789473684, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 34149; Mature: 34149

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYHTPELLPHPSVVTFGNFDGVHLGHLALLSALSGVSKLADLHRVVITFDPHPFCFLK
CCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEE
RKKDFLITDQYTKTVLLSPYADSLYFIKFDDELRSLSPEDFLKFLSSFLNLKYLINAENC
CCCCEEEECCCCCEEEECCCCCCEEEEEECHHHHCCCHHHHHHHHHHHCCEEEEEECCCC
RFGKFSSGGIAEIEKYQELLSYKAVVIKPELNDSGSRYSSTLVRTSIKDGDMLSARQLLG
CCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHEECCCCCCHHHHHHHHH
RYHSIRGIIRRDRGLASTIGFPTINIAIPEEIIKPRLGVYVVDVSIEGTIHRGIANIGKR
HHHHHHHHHHHCCCCHHHCCCCEEEEECCHHHHCCCCCEEEEEEEECCHHHHHHHHCCCC
PTTSGSGEVILEVHIFNFSDNVYGKDATVYLKAFLREEKLFPSVERLREQILADIRAVLS
CCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MNF
CCC
>Mature Secondary Structure
MQVYHTPELLPHPSVVTFGNFDGVHLGHLALLSALSGVSKLADLHRVVITFDPHPFCFLK
CCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEE
RKKDFLITDQYTKTVLLSPYADSLYFIKFDDELRSLSPEDFLKFLSSFLNLKYLINAENC
CCCCEEEECCCCCEEEECCCCCCEEEEEECHHHHCCCHHHHHHHHHHHCCEEEEEECCCC
RFGKFSSGGIAEIEKYQELLSYKAVVIKPELNDSGSRYSSTLVRTSIKDGDMLSARQLLG
CCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHEECCCCCCHHHHHHHHH
RYHSIRGIIRRDRGLASTIGFPTINIAIPEEIIKPRLGVYVVDVSIEGTIHRGIANIGKR
HHHHHHHHHHHCCCCHHHCCCCEEEEECCHHHHCCCCCEEEEEEEECCHHHHHHHHCCCC
PTTSGSGEVILEVHIFNFSDNVYGKDATVYLKAFLREEKLFPSVERLREQILADIRAVLS
CCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MNF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]