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The map label for this gene is rrmJ

Identifier: 88608723

GI number: 88608723

Start: 475908

End: 476549

Strand: Reverse

Name: rrmJ

Synonym: NSE_0546

Alternate gene names: 88608723

Gene position: 476549-475908 (Counterclockwise)

Preceding gene: 88608604

Following gene: 88608242

Centisome position: 55.48

GC content: 40.5

Gene sequence:

>642_bases
GTGAAAAACAAACTTCATCGTGTAGGTAGATGCACTGCTTCTTCAAGTCGGTGGCTTTATAGACACGTTAATGATCCTTT
CGTGAAAAAAGCGAAAGCTGAACAATACAGATCCAGAGCGGCATACAAACTTCTAGAAATAGATGAAAAGTTTAATCTCA
TTCGGAAGGGTTTTGTTGTGCTTGAGCTCGGAAGTGCACCTGGTGGTTGGAGCCAAGTAATCGCAGATATTTTGAATGGT
ACTGGACGGCTAATTGCAGTTGATTTGGCTGATATGGATCCTATTTCTGGGGTTGAGGTCGTAAAATTGGATATAGAGTT
GCAGCGTAAAGAGCTCTATGAATACATTAGCGGTGTTGAGTTAGATGTTATTGTTTCGGATCTTGCACCAAGCGCTTCTG
GCAGCAGAGTCACGGATAGCATTTCATCAATAAGATTGGCTGAGCTAGTTCTGCATTATGCAAAAAACAGCTTGAAGAAG
TTTGGTACAGTGGTAACCAAGATACTTAGAGGTAGTGAGGACGAATATAGATTTGTTAATTCACTAAGGAAGCAGTTTAA
GAAAATAGAGTATTTCAAACCGGACGCTAGTAGGAAGGCTTCTCGGGAAATATACCTTATTTTGCTTGGTAAGCTTTCTT
AG

Upstream 100 bases:

>100_bases
GAGAAAATTGCACGTATGTATCCGAATATTCATGTTATTAAATGCATGGGAGTCGGTCGTACGAGTGAGGAGATCGTTAA
GATAGTACAAGGAAAAATAA

Downstream 100 bases:

>100_bases
CTTGTCTGTTTCTATAATACGAAATCTCTTTTTTTTTGTGGAGTAAAGCGGAATCCCTACTATTCCGATTACGGGTCGTA
AAAATCTGTGGAATTTTTGA

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKNKLHRVGRCTASSSRWLYRHVNDPFVKKAKAEQYRSRAAYKLLEIDEKFNLIRKGFVVLELGSAPGGWSQVIADILNG
TGRLIAVDLADMDPISGVEVVKLDIELQRKELYEYISGVELDVIVSDLAPSASGSRVTDSISSIRLAELVLHYAKNSLKK
FGTVVTKILRGSEDEYRFVNSLRKQFKKIEYFKPDASRKASREIYLILLGKLS

Sequences:

>Translated_213_residues
MKNKLHRVGRCTASSSRWLYRHVNDPFVKKAKAEQYRSRAAYKLLEIDEKFNLIRKGFVVLELGSAPGGWSQVIADILNG
TGRLIAVDLADMDPISGVEVVKLDIELQRKELYEYISGVELDVIVSDLAPSASGSRVTDSISSIRLAELVLHYAKNSLKK
FGTVVTKILRGSEDEYRFVNSLRKQFKKIEYFKPDASRKASREIYLILLGKLS
>Mature_213_residues
MKNKLHRVGRCTASSSRWLYRHVNDPFVKKAKAEQYRSRAAYKLLEIDEKFNLIRKGFVVLELGSAPGGWSQVIADILNG
TGRLIAVDLADMDPISGVEVVKLDIELQRKELYEYISGVELDVIVSDLAPSASGSRVTDSISSIRLAELVLHYAKNSLKK
FGTVVTKILRGSEDEYRFVNSLRKQFKKIEYFKPDASRKASREIYLILLGKLS

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=221, Percent_Identity=37.10407239819, Blast_Score=141, Evalue=3e-34,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=32.9842931937173, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=33.1578947368421, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=33.1578947368421, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=33.1578947368421, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=40.8866995073892, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17553474, Length=199, Percent_Identity=33.6683417085427, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=186, Percent_Identity=32.7956989247312, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=186, Percent_Identity=32.7956989247312, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=186, Percent_Identity=32.7956989247312, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17554650, Length=194, Percent_Identity=29.8969072164948, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319796, Length=183, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=31.3131313131313, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321302, Length=101, Percent_Identity=38.6138613861386, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356387, Length=205, Percent_Identity=32.6829268292683, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=34.7150259067358, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859957, Length=191, Percent_Identity=35.0785340314136, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24647580, Length=196, Percent_Identity=31.1224489795918, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_NEOSM (Q2GDL7)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506429.1
- ProteinModelPortal:   Q2GDL7
- SMR:   Q2GDL7
- STRING:   Q2GDL7
- GeneID:   3932156
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0546
- TIGR:   NSE_0546
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q2GDL7
- ProtClustDB:   CLSK2528105
- BioCyc:   NSEN222891:NSE_0546-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24053; Mature: 24053

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: ACT_SITE 167-167 BINDING 68-68 BINDING 70-70 BINDING 88-88 BINDING 104-104 BINDING 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLHRVGRCTASSSRWLYRHVNDPFVKKAKAEQYRSRAAYKLLEIDEKFNLIRKGFVV
CCCHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LELGSAPGGWSQVIADILNGTGRLIAVDLADMDPISGVEVVKLDIELQRKELYEYISGVE
EEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCCC
LDVIVSDLAPSASGSRVTDSISSIRLAELVLHYAKNSLKKFGTVVTKILRGSEDEYRFVN
CEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SLRKQFKKIEYFKPDASRKASREIYLILLGKLS
HHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKNKLHRVGRCTASSSRWLYRHVNDPFVKKAKAEQYRSRAAYKLLEIDEKFNLIRKGFVV
CCCHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LELGSAPGGWSQVIADILNGTGRLIAVDLADMDPISGVEVVKLDIELQRKELYEYISGVE
EEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCCC
LDVIVSDLAPSASGSRVTDSISSIRLAELVLHYAKNSLKKFGTVVTKILRGSEDEYRFVN
CEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SLRKQFKKIEYFKPDASRKASREIYLILLGKLS
HHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA