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The map label for this gene is pyrG

Identifier: 88608707

GI number: 88608707

Start: 782794

End: 784440

Strand: Direct

Name: pyrG

Synonym: NSE_0877

Alternate gene names: 88608707

Gene position: 782794-784440 (Clockwise)

Preceding gene: 88608627

Following gene: 88608121

Centisome position: 91.13

GC content: 42.5

Gene sequence:

>1647_bases
TTGAATAGGAGATCCTGTGCCTTCATATTTGTTACAGGGGGTGTCTCGTCGTCTATTGGTAAGGGGCTTACGACCTCCGC
CTTGGCATCTTTGATGCAGTCTATGGGGTTTCGTGTTTGCATCAGGAAAATGGATCCCTATCTTAACGTCGATCCTGGAA
CTATGAGCCCACTGCAGCACGGTGAGGTCTTCGTCACTGAAGATGGTACTGAGGCTGATCTTGATCTTGGTCATTACGAG
CGATTTACTGGTATAAACTGCACCCACCGTGATAGCACGACGGCAGGGAGGATATATGTTGACCTTATAGAACGCGAGCG
GAATGGTGAGTATCTTGGTGCAACTGTTCAAGTTATTCCACACGTCACGAATCTTATTAAGGACTTTATTTACTCCGGGT
CTGACCAGTATGATGTGATACTGTGTGAGATTGGTGGTACAGTCGGAGATATTGAGGGGCAGCCATTTTTTGAATCCGCA
AGGCAGGTTGCCTACGAAGTTGGGAAGGAAAATGTCATATACATACATCTTACCCTTGTTCCTTACTTAGAAGCTTCTGG
TGAGCTTAAAACAAAACCTTCTCAACACTCTGTCAAGGCATTGAATGCTTTAGGTATACAACCTGATTTCTTAATATGCA
GAACACAATCAGCACAAATGTCTGCTGCAGACAAAAGGAAGATAGCTCTATTTTGTAATATTAGGGAGGGTAACGTTATA
GAAGCGCAGGACGTAGACAACATATACAAGATCCCTCTGATCTATAAAGAGCAGGGGCTTGACACAAAAATAAGTGCGCT
TCTTGGGTGCGGTCCACTAGAAGCAGGCGTATCTCACTGGAAAGAGATTGTAGGAAGAACACGATCTCTTGCAAACAGTG
GTAAAAAACTTTTTGTAGCGATAATTGGTAAGTACTCAGGTTTATGCGACGCTTACATTTCGGTTGATTCTGCTTTGCAG
CATGCTGCTTTCGCTGTAGGAGTAAATTTATCTGTCCAAATTATAGACGCACGAGGAAAAGACATGTCCGACCTTTCAAA
ATTTGACATGATACTCATCCCGGGTGGTTTTGGTAATAATGGAATAGACGGCAAGCTATTAGCAATAACGCATGCAAGAA
TCAACAATATTCCTTTTTTAGGTATTTGTTTTGGTTTTCAGTTAGCAATTTTAGAATTTGCGAGGAATGTTGTCGGAATT
AAAGATGCTGATTCAACGGAGTTTAACAAAAATTGTAAAGCACCTGTAATTTGCTTTTTGGACGAGTGGCACCGTTCAGA
CTCATCTGTGGAAAGGCGCCGTCAATCCTCACAAGTTGGGGGCACGATGCGTCTTGGTGGATATAATTGCGTTCTAAAAA
AGGAATCAAAAGCTTACGCGATCTACAACGAAACAGAGGTAATTTCAGAACGACACAGACACAGATACGAAGCCAATCCA
CATTATTTGCCTGAACTGGAGGCAAAAGGTATGCTTTTTTCTGGATATTCAGAGAAAGGTTGCGATATTCCAGAAATCCT
CGAACTCAAAGATCATCCTTGGTTTCTGGGGGTACAGTTTCACCCAGAATTCAAATCCAGGATATTTAAACCACATCCGC
TGTTTTTATCATTCGTAAGAGCAGGACTGCTACGTAAATACAGCTGA

Upstream 100 bases:

>100_bases
ACTTCGAACACCGAGGTTATAACAAATGAAGTGGAAGAAGAGTTAGAGAGGGCCCTAGAAGCAGATCATAGGGAAGCTGA
AAATGTCGACTATACCTCCT

Downstream 100 bases:

>100_bases
TACAGACGCACACACAGGAAACCTAGTCTCTTACGTGAATCTATGCTACAATTAGCCTGATGGGGGTGCCATTAAAGACC
GTGGAGGCAACAGGAGTCGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNRRSCAFIFVTGGVSSSIGKGLTTSALASLMQSMGFRVCIRKMDPYLNVDPGTMSPLQHGEVFVTEDGTEADLDLGHYE
RFTGINCTHRDSTTAGRIYVDLIERERNGEYLGATVQVIPHVTNLIKDFIYSGSDQYDVILCEIGGTVGDIEGQPFFESA
RQVAYEVGKENVIYIHLTLVPYLEASGELKTKPSQHSVKALNALGIQPDFLICRTQSAQMSAADKRKIALFCNIREGNVI
EAQDVDNIYKIPLIYKEQGLDTKISALLGCGPLEAGVSHWKEIVGRTRSLANSGKKLFVAIIGKYSGLCDAYISVDSALQ
HAAFAVGVNLSVQIIDARGKDMSDLSKFDMILIPGGFGNNGIDGKLLAITHARINNIPFLGICFGFQLAILEFARNVVGI
KDADSTEFNKNCKAPVICFLDEWHRSDSSVERRRQSSQVGGTMRLGGYNCVLKKESKAYAIYNETEVISERHRHRYEANP
HYLPELEAKGMLFSGYSEKGCDIPEILELKDHPWFLGVQFHPEFKSRIFKPHPLFLSFVRAGLLRKYS

Sequences:

>Translated_548_residues
MNRRSCAFIFVTGGVSSSIGKGLTTSALASLMQSMGFRVCIRKMDPYLNVDPGTMSPLQHGEVFVTEDGTEADLDLGHYE
RFTGINCTHRDSTTAGRIYVDLIERERNGEYLGATVQVIPHVTNLIKDFIYSGSDQYDVILCEIGGTVGDIEGQPFFESA
RQVAYEVGKENVIYIHLTLVPYLEASGELKTKPSQHSVKALNALGIQPDFLICRTQSAQMSAADKRKIALFCNIREGNVI
EAQDVDNIYKIPLIYKEQGLDTKISALLGCGPLEAGVSHWKEIVGRTRSLANSGKKLFVAIIGKYSGLCDAYISVDSALQ
HAAFAVGVNLSVQIIDARGKDMSDLSKFDMILIPGGFGNNGIDGKLLAITHARINNIPFLGICFGFQLAILEFARNVVGI
KDADSTEFNKNCKAPVICFLDEWHRSDSSVERRRQSSQVGGTMRLGGYNCVLKKESKAYAIYNETEVISERHRHRYEANP
HYLPELEAKGMLFSGYSEKGCDIPEILELKDHPWFLGVQFHPEFKSRIFKPHPLFLSFVRAGLLRKYS
>Mature_548_residues
MNRRSCAFIFVTGGVSSSIGKGLTTSALASLMQSMGFRVCIRKMDPYLNVDPGTMSPLQHGEVFVTEDGTEADLDLGHYE
RFTGINCTHRDSTTAGRIYVDLIERERNGEYLGATVQVIPHVTNLIKDFIYSGSDQYDVILCEIGGTVGDIEGQPFFESA
RQVAYEVGKENVIYIHLTLVPYLEASGELKTKPSQHSVKALNALGIQPDFLICRTQSAQMSAADKRKIALFCNIREGNVI
EAQDVDNIYKIPLIYKEQGLDTKISALLGCGPLEAGVSHWKEIVGRTRSLANSGKKLFVAIIGKYSGLCDAYISVDSALQ
HAAFAVGVNLSVQIIDARGKDMSDLSKFDMILIPGGFGNNGIDGKLLAITHARINNIPFLGICFGFQLAILEFARNVVGI
KDADSTEFNKNCKAPVICFLDEWHRSDSSVERRRQSSQVGGTMRLGGYNCVLKKESKAYAIYNETEVISERHRHRYEANP
HYLPELEAKGMLFSGYSEKGCDIPEILELKDHPWFLGVQFHPEFKSRIFKPHPLFLSFVRAGLLRKYS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=562, Percent_Identity=42.1708185053381, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI28559083, Length=562, Percent_Identity=42.1708185053381, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI221316689, Length=562, Percent_Identity=42.1708185053381, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI148491070, Length=565, Percent_Identity=40.8849557522124, Blast_Score=428, Evalue=1e-120,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=49.4464944649446, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25148299, Length=616, Percent_Identity=35.8766233766234, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322563, Length=576, Percent_Identity=39.7569444444444, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=579, Percent_Identity=39.7236614853195, Blast_Score=412, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24664469, Length=564, Percent_Identity=39.7163120567376, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI21357815, Length=510, Percent_Identity=38.6274509803922, Blast_Score=367, Evalue=1e-101,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_NEOSM (Q2GCQ2)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506744.1
- HSSP:   P17812
- ProteinModelPortal:   Q2GCQ2
- SMR:   Q2GCQ2
- STRING:   Q2GCQ2
- GeneID:   3931541
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0877
- TIGR:   NSE_0877
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- BioCyc:   NSEN222891:NSE_0877-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60777; Mature: 60777

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 383-383 ACT_SITE 521-521 ACT_SITE 523-523

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRSCAFIFVTGGVSSSIGKGLTTSALASLMQSMGFRVCIRKMDPYLNVDPGTMSPLQH
CCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCCC
GEVFVTEDGTEADLDLGHYERFTGINCTHRDSTTAGRIYVDLIERERNGEYLGATVQVIP
CCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEHHHHHHH
HVTNLIKDFIYSGSDQYDVILCEIGGTVGDIEGQPFFESARQVAYEVGKENVIYIHLTLV
HHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
PYLEASGELKTKPSQHSVKALNALGIQPDFLICRTQSAQMSAADKRKIALFCNIREGNVI
EEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEE
EAQDVDNIYKIPLIYKEQGLDTKISALLGCGPLEAGVSHWKEIVGRTRSLANSGKKLFVA
ECCCCCCEEECCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IIGKYSGLCDAYISVDSALQHAAFAVGVNLSVQIIDARGKDMSDLSKFDMILIPGGFGNN
EECCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCCHHHHHHCCEEEEECCCCCC
GIDGKLLAITHARINNIPFLGICFGFQLAILEFARNVVGIKDADSTEFNKNCKAPVICFL
CCCCEEEEEEECHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
DEWHRSDSSVERRRQSSQVGGTMRLGGYNCVLKKESKAYAIYNETEVISERHRHRYEANP
EHHCCCCHHHHHHHHHHHCCCEEEECCEEEEEEECCCEEEEECHHHHHHHHHHHCCCCCC
HYLPELEAKGMLFSGYSEKGCDIPEILELKDHPWFLGVQFHPEFKSRIFKPHPLFLSFVR
CCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEEECCHHHHCCCCCCHHHHHHHH
AGLLRKYS
HHHHHCCC
>Mature Secondary Structure
MNRRSCAFIFVTGGVSSSIGKGLTTSALASLMQSMGFRVCIRKMDPYLNVDPGTMSPLQH
CCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCCC
GEVFVTEDGTEADLDLGHYERFTGINCTHRDSTTAGRIYVDLIERERNGEYLGATVQVIP
CCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEHHHHHHH
HVTNLIKDFIYSGSDQYDVILCEIGGTVGDIEGQPFFESARQVAYEVGKENVIYIHLTLV
HHHHHHHHHHHCCCCCEEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
PYLEASGELKTKPSQHSVKALNALGIQPDFLICRTQSAQMSAADKRKIALFCNIREGNVI
EEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEE
EAQDVDNIYKIPLIYKEQGLDTKISALLGCGPLEAGVSHWKEIVGRTRSLANSGKKLFVA
ECCCCCCEEECCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IIGKYSGLCDAYISVDSALQHAAFAVGVNLSVQIIDARGKDMSDLSKFDMILIPGGFGNN
EECCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCCHHHHHHCCEEEEECCCCCC
GIDGKLLAITHARINNIPFLGICFGFQLAILEFARNVVGIKDADSTEFNKNCKAPVICFL
CCCCEEEEEEECHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
DEWHRSDSSVERRRQSSQVGGTMRLGGYNCVLKKESKAYAIYNETEVISERHRHRYEANP
EHHCCCCHHHHHHHHHHHCCCEEEECCEEEEEEECCCEEEEECHHHHHHHHHHHCCCCCC
HYLPELEAKGMLFSGYSEKGCDIPEILELKDHPWFLGVQFHPEFKSRIFKPHPLFLSFVR
CCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEEECCHHHHCCCCCCHHHHHHHH
AGLLRKYS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA