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The map label for this gene is lipB [H]

Identifier: 88608665

GI number: 88608665

Start: 505948

End: 506742

Strand: Reverse

Name: lipB [H]

Synonym: NSE_0580

Alternate gene names: 88608665

Gene position: 506742-505948 (Counterclockwise)

Preceding gene: 88608437

Following gene: 88608553

Centisome position: 58.99

GC content: 41.76

Gene sequence:

>795_bases
ATGAAGATTACGGTTTTAGGTTGTGGCAATTCTTCAGGTGTGCCAGTTTTAGGTTGTGCATGTTCGGTATGTTTATCAAG
TAATCCGCTTGATACGAGAACGAGGTGCTCTGTTTTTGTGGAGACTGCTTCTACCCGCTTACTCATAGATGCATCACCAG
ATTTGCGAGCTCAAGCGATGCGGGAGGGGTTCTCGAGTGTGGATCAGGTTTTTTACACTCACATACACTTCGATCATCAC
GCTGGAACTCACGAGCTTTCTTCCTTTGCCACCTCAGAAGGGAATTGTATTCATGTATTCAGTGATGAATTTACTATCTC
TCATATAGAGAGTCTCTATGGATATCTTTTCAAGCCGGAACATTTAAGCAATGCAATGTGGAAAAGATGTTACCTTAGTG
CAAAGGTTGTCCCGTACTACGAGGAGTTTGCTGTGGGGGATATGCTTTGCATGATTTTCCCCCAAACACACGGCAGTATA
AACAGTTGTGGAATTATTCTGAATGGAAAGTTTGCATATTGTACTGATGTTCGTTTAATACCGGATCGTGCCCTTGATAT
TATGAAGTCCCTCGAGCTAGAGGTTTTTATTATTGAGTGTTTTGACTATCAAAGTTCCTATGCTCACTCCAATTTTTCTG
AAGCTTTGGAGTATATAAATCTCATACAACCGAAAAGTGCTTTTTTTACCCACATGACGCATAAGTTAGCGTATGACGAT
CTGCTTACCAGGCTAAAAGTCAGTGGTGTTAGTCATGTGATGCCAGCACACGATGGTCTCTCTTTGGAGATATGA

Upstream 100 bases:

>100_bases
TTCTCTCGCTGGAGGGTTTTGATGACCATCCCGATGCTTGCAATGAGAGAATACTAGAATCTATACAAGCGGCTTGGATG
TCTGAGCGGGAAGACCGGGA

Downstream 100 bases:

>100_bases
TCTTTGAGACAGTTAGTTTGCTACGTGTTGGTGGGGTTGTTATCTTTCCAACTGAGACTGTGTATGCGCTTGCTTGTGAT
GCATCAAATTATCGTGCCAT

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKITVLGCGNSSGVPVLGCACSVCLSSNPLDTRTRCSVFVETASTRLLIDASPDLRAQAMREGFSSVDQVFYTHIHFDHH
AGTHELSSFATSEGNCIHVFSDEFTISHIESLYGYLFKPEHLSNAMWKRCYLSAKVVPYYEEFAVGDMLCMIFPQTHGSI
NSCGIILNGKFAYCTDVRLIPDRALDIMKSLELEVFIIECFDYQSSYAHSNFSEALEYINLIQPKSAFFTHMTHKLAYDD
LLTRLKVSGVSHVMPAHDGLSLEI

Sequences:

>Translated_264_residues
MKITVLGCGNSSGVPVLGCACSVCLSSNPLDTRTRCSVFVETASTRLLIDASPDLRAQAMREGFSSVDQVFYTHIHFDHH
AGTHELSSFATSEGNCIHVFSDEFTISHIESLYGYLFKPEHLSNAMWKRCYLSAKVVPYYEEFAVGDMLCMIFPQTHGSI
NSCGIILNGKFAYCTDVRLIPDRALDIMKSLELEVFIIECFDYQSSYAHSNFSEALEYINLIQPKSAFFTHMTHKLAYDD
LLTRLKVSGVSHVMPAHDGLSLEI
>Mature_264_residues
MKITVLGCGNSSGVPVLGCACSVCLSSNPLDTRTRCSVFVETASTRLLIDASPDLRAQAMREGFSSVDQVFYTHIHFDHH
AGTHELSSFATSEGNCIHVFSDEFTISHIESLYGYLFKPEHLSNAMWKRCYLSAKVVPYYEEFAVGDMLCMIFPQTHGSI
NSCGIILNGKFAYCTDVRLIPDRALDIMKSLELEVFIIECFDYQSSYAHSNFSEALEYINLIQPKSAFFTHMTHKLAYDD
LLTRLKVSGVSHVMPAHDGLSLEI

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 29492; Mature: 29492

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVLGCGNSSGVPVLGCACSVCLSSNPLDTRTRCSVFVETASTRLLIDASPDLRAQAM
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEECCCCHHHHHH
REGFSSVDQVFYTHIHFDHHAGTHELSSFATSEGNCIHVFSDEFTISHIESLYGYLFKPE
HHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCH
HLSNAMWKRCYLSAKVVPYYEEFAVGDMLCMIFPQTHGSINSCGIILNGKFAYCTDVRLI
HHHHHHHHHHHHCCEECCCHHHHHHHCEEEEEEECCCCCCCCCEEEEECCEEEEEEEEEC
PDRALDIMKSLELEVFIIECFDYQSSYAHSNFSEALEYINLIQPKSAFFTHMTHKLAYDD
CHHHHHHHHHCCEEEEEEEEECCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLTRLKVSGVSHVMPAHDGLSLEI
HHHHHHHCCCHHCCCCCCCCEEEC
>Mature Secondary Structure
MKITVLGCGNSSGVPVLGCACSVCLSSNPLDTRTRCSVFVETASTRLLIDASPDLRAQAM
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEECCCCHHHHHH
REGFSSVDQVFYTHIHFDHHAGTHELSSFATSEGNCIHVFSDEFTISHIESLYGYLFKPE
HHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCH
HLSNAMWKRCYLSAKVVPYYEEFAVGDMLCMIFPQTHGSINSCGIILNGKFAYCTDVRLI
HHHHHHHHHHHHCCEECCCHHHHHHHCEEEEEEECCCCCCCCCEEEEECCEEEEEEEEEC
PDRALDIMKSLELEVFIIECFDYQSSYAHSNFSEALEYINLIQPKSAFFTHMTHKLAYDD
CHHHHHHHHHCCEEEEEEEEECCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLTRLKVSGVSHVMPAHDGLSLEI
HHHHHHHCCCHHCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]