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The map label for this gene is braB [H]

Identifier: 88608633

GI number: 88608633

Start: 747634

End: 748776

Strand: Direct

Name: braB [H]

Synonym: NSE_0845

Alternate gene names: 88608633

Gene position: 747634-748776 (Clockwise)

Preceding gene: 88608543

Following gene: 88608479

Centisome position: 87.03

GC content: 41.64

Gene sequence:

>1143_bases
ATGTTTGTCTCCAAATCAACACAAGTCGCTATTGCCTCTTTAACTGTTTTCGCGATTTTCTTCGGTGCAGGAAATCTCAT
ATATCCAACTTTTGTTGGCCTGGAAACAAAAAGTAACTTCGCATATGGCTTTCTTGGATTTGCATCCACAGGAATTTTCT
TACCATTATTGGCGCTGATAAGCATGATCAAGGCTGGATGCAATATGGATAAATTTTTCTCCTTGGCAGGAAAGCAAGTT
GGCACAATATTAGTGATGCTAATACTGCTATTAATGGGACCACTCGGTGGAATCCCTAGGTGCATCATAGTTTCTTTCGA
CGCACTCACAGTTATGAGCAGTAGCATCCGTTTTCTCCATTTTAATTTATTTTTCGGCGTGCTGACATTTCTGCTTGTCC
TACGCGATAGTGAAGTTGTTCAGATCGTTGGTAAGTGGCTCACACCTGCATTGATAATGGGAATACTAGTGATAGTAATT
GCTGGGATATCATCAAATCAGGCAATGTCCGACGCTACACTTTCTCGATTTCCTTCGTTTACATTTGCAGTCTTCGAGGG
TTATCAAACGATGGACGCGTGTGCAGCAATTTTTTTCGCAAAAATCATACTGGATTACTTTAAGAAGGAAGCGCCAGAAG
GTAATGCTTTGAACTACTCTTTACTTTCTTCGATACTCGGCTTTTTCCTTTTGGGAATTGTATATGCAGCGCTGCTTTTC
TTGGGCGCAAAGTACGCAACTAATCTCGACGAAATACCAAAAGCAAGCTTATTCAACTCTCTAACATCTCTGGTTGCAGG
CAAAGCATCATCAGTGGTGGTGGCATTTACTGTCTTTTTTGCTTGTCTTACCACCGTGATTGCATTGGTACAATTAATAT
CAAAATGGCTCACAGAGCGCACTGGCATTGACCTTAGACTATCTGTTTGTGGCACTGTCATTTGTAGCATGATTTCCGCT
TCGTTTGGATTTCAGGCTTTAACCACTTTTCTGGTGGGTATCTTGAGCTTACTTTATCCTGCCCTTATTGTACTCATCTT
ATGCAATTTAGCTTCTAGCAGTTCGCAGTACGTGCGCAAGTATTCTCCAGTGATCTTCTGGCTCACAGCAGCGGTTAGTG
CGTATGTAAGTATAGGGGTATAA

Upstream 100 bases:

>100_bases
TGCAGAAGATTTGGCTCTACTAGCACTTCAACAGAAAGATTTCAAAAATGAATCTCAACCTGTTGTCCCACTGTACATAT
AACCAAGATCACAAATTTCT

Downstream 100 bases:

>100_bases
AGCCACCAAAAAGGTTAAAAATATTAAAGACAGCCTTGATATTACTCTGAAAGAGATATTATCATGATTCTCGCCGGTGG
TTTCAAAAATGGGTAACCTT

Product: branched-chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MFVSKSTQVAIASLTVFAIFFGAGNLIYPTFVGLETKSNFAYGFLGFASTGIFLPLLALISMIKAGCNMDKFFSLAGKQV
GTILVMLILLLMGPLGGIPRCIIVSFDALTVMSSSIRFLHFNLFFGVLTFLLVLRDSEVVQIVGKWLTPALIMGILVIVI
AGISSNQAMSDATLSRFPSFTFAVFEGYQTMDACAAIFFAKIILDYFKKEAPEGNALNYSLLSSILGFFLLGIVYAALLF
LGAKYATNLDEIPKASLFNSLTSLVAGKASSVVVAFTVFFACLTTVIALVQLISKWLTERTGIDLRLSVCGTVICSMISA
SFGFQALTTFLVGILSLLYPALIVLILCNLASSSSQYVRKYSPVIFWLTAAVSAYVSIGV

Sequences:

>Translated_380_residues
MFVSKSTQVAIASLTVFAIFFGAGNLIYPTFVGLETKSNFAYGFLGFASTGIFLPLLALISMIKAGCNMDKFFSLAGKQV
GTILVMLILLLMGPLGGIPRCIIVSFDALTVMSSSIRFLHFNLFFGVLTFLLVLRDSEVVQIVGKWLTPALIMGILVIVI
AGISSNQAMSDATLSRFPSFTFAVFEGYQTMDACAAIFFAKIILDYFKKEAPEGNALNYSLLSSILGFFLLGIVYAALLF
LGAKYATNLDEIPKASLFNSLTSLVAGKASSVVVAFTVFFACLTTVIALVQLISKWLTERTGIDLRLSVCGTVICSMISA
SFGFQALTTFLVGILSLLYPALIVLILCNLASSSSQYVRKYSPVIFWLTAAVSAYVSIGV
>Mature_380_residues
MFVSKSTQVAIASLTVFAIFFGAGNLIYPTFVGLETKSNFAYGFLGFASTGIFLPLLALISMIKAGCNMDKFFSLAGKQV
GTILVMLILLLMGPLGGIPRCIIVSFDALTVMSSSIRFLHFNLFFGVLTFLLVLRDSEVVQIVGKWLTPALIMGILVIVI
AGISSNQAMSDATLSRFPSFTFAVFEGYQTMDACAAIFFAKIILDYFKKEAPEGNALNYSLLSSILGFFLLGIVYAALLF
LGAKYATNLDEIPKASLFNSLTSLVAGKASSVVVAFTVFFACLTTVIALVQLISKWLTERTGIDLRLSVCGTVICSMISA
SFGFQALTTFLVGILSLLYPALIVLILCNLASSSSQYVRKYSPVIFWLTAAVSAYVSIGV

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=357, Percent_Identity=26.0504201680672, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 40875; Mature: 40875

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFVSKSTQVAIASLTVFAIFFGAGNLIYPTFVGLETKSNFAYGFLGFASTGIFLPLLALI
CCCCCCHHHHHHHHHHHHHHHHCCCEECHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
SMIKAGCNMDKFFSLAGKQVGTILVMLILLLMGPLGGIPRCIIVSFDALTVMSSSIRFLH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHHHHHHHHHHHHH
FNLFFGVLTFLLVLRDSEVVQIVGKWLTPALIMGILVIVIAGISSNQAMSDATLSRFPSF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
TFAVFEGYQTMDACAAIFFAKIILDYFKKEAPEGNALNYSLLSSILGFFLLGIVYAALLF
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAKYATNLDEIPKASLFNSLTSLVAGKASSVVVAFTVFFACLTTVIALVQLISKWLTER
HHHHHHCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIDLRLSVCGTVICSMISASFGFQALTTFLVGILSLLYPALIVLILCNLASSSSQYVRK
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YSPVIFWLTAAVSAYVSIGV
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]