Click here to switch to the map view.

The map label for this gene is nuoM

Identifier: 88608616

GI number: 88608616

Start: 611895

End: 613256

Strand: Direct

Name: nuoM

Synonym: NSE_0691

Alternate gene names: 88608616

Gene position: 611895-613256 (Clockwise)

Preceding gene: 88608724

Following gene: 88608865

Centisome position: 71.23

GC content: 42.51

Gene sequence:

>1362_bases
ATGACACCGCTGTTTCTACTGCTTCCCCTGTTTGCATCACTGCTTGTACCGTTCTTTGTGTCGAACAGCAAATACTATTT
TTCCATTCTCTTTTCTGTATCCACATTCTTGGCTTCGGTCATCGTAACCGCGCGTGCTGGTTCTGAGTTTATAGTCTTTC
AACTGGGGACGATCACACTCAAAGCGGATGAACTTTCCTGCGTAATGCTGCTTTTGACGTCCTTGCTTACGATGCTCTGC
ATGATCATGGCAAAGGATAAACCCCCTGGTTACTTTAGCCTGTTGTTTTTGCTTGAATTCGTCCTTACGGTGTTCTTCTT
AGCAACGGATTTACTTACTTTCTACATAATGTTTGAGCTTTCGCTCATCCCAATTTTTTTCATTATTGGTCTTTGGGGAG
GAAAGAAGAGAGTATATGCAGCGTTTAAGATCTTTTTATACACACTGTTTGGATCGATTGGATTTTTGATATCGATTTTG
TATCTATTTGCAGAACTAGGGACACTCGAAATTAGCTCCTTGGTACATTTGGTAGAGAACACAATCCCGATTGCAGCACA
AAAACTTATTTGGATTGCCTTCTTCCTGGCATTTGCCGTCAAAATACCGATGTTCCCATTTCATACTTGGCTGCCGGATG
CGCATGTTCAGGCACCAACTGAAGGTTCAGTGATGCTTGCGGGTGTACTGATAAAACTAGGGGCATATGGAATGCTCAAA
GTCTTGTTACCAATTTTTCCCTCACTTAGCAGAGAATTCTCTACTTTAGTACTTTCACTAAGCGCAATTGCAGTGATCTA
TACCTCGTTAGTTGCACTTGCACAGACAGATATAAAGAAACTCATTGCATACTCGTCGATTGCACATATGGGAATCGTGA
CTGCTGGTCTTTTCGCATTCAATATTGAGGGATACAAAGGAGCGGTATTTCAAATGCTAAGTCATGGTTTGGTCTCCTCA
GGGTTATTTTTTTGCATAGGCTACATCTACGACCGCAGTGCAACAAGACAAATCGCCGACCACTCCGGTATAATGAATTT
TGCTCCCAGGCTAGGGTTTGCATTTATGATCTTTTCACTTGCGGCAATTGGCCTTCCTGGTACCGCAGGTTTCGTGGGTG
AGTTCCTAGCATTTACAGGGCTATTTCAAAGCATGGAGACGTTTGCGATACTCTGTGCTACTGGTGTAGTTTTAGGAGCT
TGTTATATGCTCTACCTTTGCAAACGAATCATATGGGGCACTCCTGAAGGCAAAATCTTTCAAGATATTCACCTACACGA
GTCTATACCACTCATTACTTTAATCATCCTAGTGATGTTTCTGGGTCTCCGCCCGAGCTTTCTTCTTAATTTTTTGAGAT
GA

Upstream 100 bases:

>100_bases
AGACCACATTTGAGATGACAGAATTTATTTACTTGCAATATCAAGGATGGTACAATCTATGCTCATTCGTTGTTCAAAAG
GTGTTATCCCTAGCCAAATA

Downstream 100 bases:

>100_bases
ATCAATACCTGTTTTTACTACCTGAAATTACACTTTTTACTCTTTCCTGTTTACTGCTTTTCTTAAAGAGCAGGAATGAA
TTTGGACTAATTGCAGTATT

Product: NADH dehydrogenase I subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTPLFLLLPLFASLLVPFFVSNSKYYFSILFSVSTFLASVIVTARAGSEFIVFQLGTITLKADELSCVMLLLTSLLTMLC
MIMAKDKPPGYFSLLFLLEFVLTVFFLATDLLTFYIMFELSLIPIFFIIGLWGGKKRVYAAFKIFLYTLFGSIGFLISIL
YLFAELGTLEISSLVHLVENTIPIAAQKLIWIAFFLAFAVKIPMFPFHTWLPDAHVQAPTEGSVMLAGVLIKLGAYGMLK
VLLPIFPSLSREFSTLVLSLSAIAVIYTSLVALAQTDIKKLIAYSSIAHMGIVTAGLFAFNIEGYKGAVFQMLSHGLVSS
GLFFCIGYIYDRSATRQIADHSGIMNFAPRLGFAFMIFSLAAIGLPGTAGFVGEFLAFTGLFQSMETFAILCATGVVLGA
CYMLYLCKRIIWGTPEGKIFQDIHLHESIPLITLIILVMFLGLRPSFLLNFLR

Sequences:

>Translated_453_residues
MTPLFLLLPLFASLLVPFFVSNSKYYFSILFSVSTFLASVIVTARAGSEFIVFQLGTITLKADELSCVMLLLTSLLTMLC
MIMAKDKPPGYFSLLFLLEFVLTVFFLATDLLTFYIMFELSLIPIFFIIGLWGGKKRVYAAFKIFLYTLFGSIGFLISIL
YLFAELGTLEISSLVHLVENTIPIAAQKLIWIAFFLAFAVKIPMFPFHTWLPDAHVQAPTEGSVMLAGVLIKLGAYGMLK
VLLPIFPSLSREFSTLVLSLSAIAVIYTSLVALAQTDIKKLIAYSSIAHMGIVTAGLFAFNIEGYKGAVFQMLSHGLVSS
GLFFCIGYIYDRSATRQIADHSGIMNFAPRLGFAFMIFSLAAIGLPGTAGFVGEFLAFTGLFQSMETFAILCATGVVLGA
CYMLYLCKRIIWGTPEGKIFQDIHLHESIPLITLIILVMFLGLRPSFLLNFLR
>Mature_452_residues
TPLFLLLPLFASLLVPFFVSNSKYYFSILFSVSTFLASVIVTARAGSEFIVFQLGTITLKADELSCVMLLLTSLLTMLCM
IMAKDKPPGYFSLLFLLEFVLTVFFLATDLLTFYIMFELSLIPIFFIIGLWGGKKRVYAAFKIFLYTLFGSIGFLISILY
LFAELGTLEISSLVHLVENTIPIAAQKLIWIAFFLAFAVKIPMFPFHTWLPDAHVQAPTEGSVMLAGVLIKLGAYGMLKV
LLPIFPSLSREFSTLVLSLSAIAVIYTSLVALAQTDIKKLIAYSSIAHMGIVTAGLFAFNIEGYKGAVFQMLSHGLVSSG
LFFCIGYIYDRSATRQIADHSGIMNFAPRLGFAFMIFSLAAIGLPGTAGFVGEFLAFTGLFQSMETFAILCATGVVLGAC
YMLYLCKRIIWGTPEGKIFQDIHLHESIPLITLIILVMFLGLRPSFLLNFLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=362, Percent_Identity=36.4640883977901, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI251831117, Length=285, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788613, Length=379, Percent_Identity=39.3139841688654, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1788827, Length=352, Percent_Identity=28.4090909090909, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788831, Length=362, Percent_Identity=23.7569060773481, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788829, Length=468, Percent_Identity=23.7179487179487, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788614, Length=488, Percent_Identity=25.8196721311475, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693160, Length=362, Percent_Identity=26.7955801104972, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367154, Length=292, Percent_Identity=25, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50062; Mature: 49931

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLFLLLPLFASLLVPFFVSNSKYYFSILFSVSTFLASVIVTARAGSEFIVFQLGTITL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
KADELSCVMLLLTSLLTMLCMIMAKDKPPGYFSLLFLLEFVLTVFFLATDLLTFYIMFEL
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIPIFFIIGLWGGKKRVYAAFKIFLYTLFGSIGFLISILYLFAELGTLEISSLVHLVEN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
TIPIAAQKLIWIAFFLAFAVKIPMFPFHTWLPDAHVQAPTEGSVMLAGVLIKLGAYGMLK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VLLPIFPSLSREFSTLVLSLSAIAVIYTSLVALAQTDIKKLIAYSSIAHMGIVTAGLFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
NIEGYKGAVFQMLSHGLVSSGLFFCIGYIYDRSATRQIADHSGIMNFAPRLGFAFMIFSL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AAIGLPGTAGFVGEFLAFTGLFQSMETFAILCATGVVLGACYMLYLCKRIIWGTPEGKIF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QDIHLHESIPLITLIILVMFLGLRPSFLLNFLR
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHC
>Mature Secondary Structure 
TPLFLLLPLFASLLVPFFVSNSKYYFSILFSVSTFLASVIVTARAGSEFIVFQLGTITL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEE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HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA