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The map label for this gene is miaB

Identifier: 88608596

GI number: 88608596

Start: 737392

End: 738807

Strand: Direct

Name: miaB

Synonym: NSE_0832

Alternate gene names: 88608596

Gene position: 737392-738807 (Clockwise)

Preceding gene: 88608832

Following gene: 88608474

Centisome position: 85.84

GC content: 40.4

Gene sequence:

>1416_bases
TTGCTGTTATACTTAGCAGACGTAGTCATATTTTCTCACGCGAAGGTTTACATGGAAAAAATAGAGAAAAAAAACAACAG
TCTGAAAAAGTTCCATATTAAGACTTATGGCTGCCAGATGAATGTTTACGATTCAGAAATGATTGAAAAAATCGTTAGCG
GACTTGGGTTCACACTCTCAGAAAGAGCTGAGGATGCAGACTTAATAATTCTAAATACCTGTAACATTAGGGAAAAAGCA
GCTGAGAAACTTTACTCAGAGCTTGGGCAGATAAGACTTCTACAAAAAAAGAAGCAAGAGAGAATTCTCATCGTGGTTGC
TGGTTGTGTTGCACAAGCTGAAGGCGAAGAGATAATGCGTAGAGCAGAAAATGTTGACGTAGTTGTTGGTCCACAGAGCA
TCCACTCGTTGCCTGAACTTATTGCAAAAGTCAACAGACAATCCGGAAAAGCAATAAAAATGGAATTTGACCCTATAGAA
AAATTTGACTACTTAGCAGAAGAAACTAGGAAAAGGCGGGTACCTCAGTCATCAGCTTTTCTCTCTATACAAGAGGGTTG
TGATAAATTCTGTGCGTTCTGTGTTGTCCCGTACACACGAGGAGCTGAGTATTCCAGATCAACAGAAGAAGTCTACCGCG
AGGCATTAAGCCTAACAACCAAAGGCGTTAAAGAGATCACCTTACTTGGACAAAACGTGAATGGCTACCATGGGACATTG
GACTCGGGAAATAAGGTACTTAACTTAGGACAACTAATTTCACGCTTAGGTAAGATTCCATCATTAAAGAGAATACGCTA
CACCACCTCACATCCTGTCGATATGCATAAAGAGCTTTATGATGCACACGCTAATGAAAGTAAGCTTATGCCATTCGTAC
ATCTACCGGTCCAATCCGGATCAGACAAAATCCTAAAACAAATGAACAGGAAATACACCACCGCAGACTATCTCAAAATC
ATTAACGAGTTCCAGAACGCACGAAGCGATATCGCTTTTTCATCGGATTTTATAGTGGGATTCCCCGGTGAAAGCGACGA
CGATTTTCAGCAGACACTCGCATTGATAGAACAAGTAAACTACGCACAATGCTATAGCTTTAAATACAGTCCAAGACCTG
GTACCCCAGGTGCTACTTATCCACAAATCTCTGAAGAAACAAAAAACACAAGGCTCCAAAAACTACAACAATTGCTCAAG
GAAAAACAATTAGAGTTCAACAAAAAAATGATTGGAAAAACCGTTACGGTCCTATTCGACAAAAAGCACCCAGACAAAAT
CTCTGGAAGGACCGAATATATGCAACAAGTCTTTTCTGATGATAGCAATTTGCTGGATAAAATAGTCACCATGCGGGTAG
AAGATGCATCGACATTCACGCTAAAATGCACTGCAGAAGACATCATTTCGACCTAG

Upstream 100 bases:

>100_bases
AAGGAAGAAGTACAGATTCGGTACTATTTGGAAAAATCATAAAATAATCAGCTAGTCTATATTGCACTAGCACAAACCAA
ACTTCTTAAAGAATTGCGCT

Downstream 100 bases:

>100_bases
TGAGCATATCTTATATGGTTCAAATGCGCGCAACAAATCTTCCGCTACAACACTAATCCGCTTGGCAAAATAAATAATAC
CTTGGTAGAACGTGCATCGA

Product: tRNA-i(6)A37 modification enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLLYLADVVIFSHAKVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLSERAEDADLIILNTCNIREKA
AEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMRRAENVDVVVGPQSIHSLPELIAKVNRQSGKAIKMEFDPIE
KFDYLAEETRKRRVPQSSAFLSIQEGCDKFCAFCVVPYTRGAEYSRSTEEVYREALSLTTKGVKEITLLGQNVNGYHGTL
DSGNKVLNLGQLISRLGKIPSLKRIRYTTSHPVDMHKELYDAHANESKLMPFVHLPVQSGSDKILKQMNRKYTTADYLKI
INEFQNARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVNYAQCYSFKYSPRPGTPGATYPQISEETKNTRLQKLQQLLK
EKQLEFNKKMIGKTVTVLFDKKHPDKISGRTEYMQQVFSDDSNLLDKIVTMRVEDASTFTLKCTAEDIIST

Sequences:

>Translated_471_residues
MLLYLADVVIFSHAKVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLSERAEDADLIILNTCNIREKA
AEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMRRAENVDVVVGPQSIHSLPELIAKVNRQSGKAIKMEFDPIE
KFDYLAEETRKRRVPQSSAFLSIQEGCDKFCAFCVVPYTRGAEYSRSTEEVYREALSLTTKGVKEITLLGQNVNGYHGTL
DSGNKVLNLGQLISRLGKIPSLKRIRYTTSHPVDMHKELYDAHANESKLMPFVHLPVQSGSDKILKQMNRKYTTADYLKI
INEFQNARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVNYAQCYSFKYSPRPGTPGATYPQISEETKNTRLQKLQQLLK
EKQLEFNKKMIGKTVTVLFDKKHPDKISGRTEYMQQVFSDDSNLLDKIVTMRVEDASTFTLKCTAEDIIST
>Mature_471_residues
MLLYLADVVIFSHAKVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLSERAEDADLIILNTCNIREKA
AEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMRRAENVDVVVGPQSIHSLPELIAKVNRQSGKAIKMEFDPIE
KFDYLAEETRKRRVPQSSAFLSIQEGCDKFCAFCVVPYTRGAEYSRSTEEVYREALSLTTKGVKEITLLGQNVNGYHGTL
DSGNKVLNLGQLISRLGKIPSLKRIRYTTSHPVDMHKELYDAHANESKLMPFVHLPVQSGSDKILKQMNRKYTTADYLKI
INEFQNARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVNYAQCYSFKYSPRPGTPGATYPQISEETKNTRLQKLQQLLK
EKQLEFNKKMIGKTVTVLFDKKHPDKISGRTEYMQQVFSDDSNLLDKIVTMRVEDASTFTLKCTAEDIIST

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=447, Percent_Identity=31.3199105145414, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI28872782, Length=448, Percent_Identity=31.25, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI93277076, Length=377, Percent_Identity=27.8514588859416, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=42.7272727272727, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1787057, Length=410, Percent_Identity=29.0243902439024, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=32.5431034482759, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=330, Percent_Identity=26.0606060606061, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=31.554524361949, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI19922432, Length=449, Percent_Identity=26.5033407572383, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NEOSM (Q2GCU4)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506702.1
- ProteinModelPortal:   Q2GCU4
- SMR:   Q2GCU4
- STRING:   Q2GCU4
- GeneID:   3932248
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0832
- TIGR:   NSE_0832
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q2GCU4
- ProtClustDB:   CLSK2528018
- BioCyc:   NSEN222891:NSE_0832-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53532; Mature: 53532

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLYLADVVIFSHAKVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLS
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECEEEECCHHHHHHHHHHHCCCCHH
ERAEDADLIILNTCNIREKAAEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCHHHHHH
RAENVDVVVGPQSIHSLPELIAKVNRQSGKAIKMEFDPIEKFDYLAEETRKRRVPQSSAF
HHCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHH
LSIQEGCDKFCAFCVVPYTRGAEYSRSTEEVYREALSLTTKGVKEITLLGQNVNGYHGTL
EEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCC
DSGNKVLNLGQLISRLGKIPSLKRIRYTTSHPVDMHKELYDAHANESKLMPFVHLPVQSG
CCCCHHHHHHHHHHHHCCCCCCHHEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCC
SDKILKQMNRKYTTADYLKIINEFQNARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVN
CHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEECCCEEEECCCCCCHHHHHHHHHHHHCC
YAQCYSFKYSPRPGTPGATYPQISEETKNTRLQKLQQLLKEKQLEFNKKMIGKTVTVLFD
HHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
KKHPDKISGRTEYMQQVFSDDSNLLDKIVTMRVEDASTFTLKCTAEDIIST
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHEEECCCEEEEEEEHHHHHCC
>Mature Secondary Structure
MLLYLADVVIFSHAKVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLS
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECEEEECCHHHHHHHHHHHCCCCHH
ERAEDADLIILNTCNIREKAAEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCHHHHHH
RAENVDVVVGPQSIHSLPELIAKVNRQSGKAIKMEFDPIEKFDYLAEETRKRRVPQSSAF
HHCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHH
LSIQEGCDKFCAFCVVPYTRGAEYSRSTEEVYREALSLTTKGVKEITLLGQNVNGYHGTL
EEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCC
DSGNKVLNLGQLISRLGKIPSLKRIRYTTSHPVDMHKELYDAHANESKLMPFVHLPVQSG
CCCCHHHHHHHHHHHHCCCCCCHHEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCC
SDKILKQMNRKYTTADYLKIINEFQNARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVN
CHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEECCCEEEECCCCCCHHHHHHHHHHHHCC
YAQCYSFKYSPRPGTPGATYPQISEETKNTRLQKLQQLLKEKQLEFNKKMIGKTVTVLFD
HHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
KKHPDKISGRTEYMQQVFSDDSNLLDKIVTMRVEDASTFTLKCTAEDIIST
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHEEECCCEEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA