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The map label for this gene is truA

Identifier: 88608581

GI number: 88608581

Start: 17293

End: 18024

Strand: Direct

Name: truA

Synonym: NSE_0020

Alternate gene names: 88608581

Gene position: 17293-18024 (Clockwise)

Preceding gene: 88608674

Following gene: 88608703

Centisome position: 2.01

GC content: 40.85

Gene sequence:

>732_bases
ATGCGCTACAAGGCAATAGTTGAATATGTTGGTACTGGTTTTTCTGGCTGGCAGAAACAAATGTCCGCTCCATCTGTGCA
GGAAGAGTTGGAATCAGTCCTCAGTTTTTTGTTAAAGGAGAAAATAGCAGTTAATGTTGCTGGAAGAACAGATGCAGGAG
TGCACGCTCTCGGACAGGTCTTTCATTTTGATGCACGTGAATCTACTTTGCAGCCTTTTCAAATAGTAAATGCGGTAAAT
TATCATTTGAAAGAGAAGTTAATAGTTCTGCTGAAGATAGAGATTGTTGATGAAACCTTTGATGCCAGATTTTCTGCTAT
TAGGAGACACTATCAATACAAAATCACAAACAGAAAGACACCACTGGCAGTATTTAAAAACCGCTCCTGGCATGTGCCGC
AGACTTTAAATCTGGATCTCATTAGGGAGCAAGCTCAATATCTTGTTGGAAAACACGATTTTCAGAGTTTTCGTTCATCC
AAATGTGGTGCTTCTAACGCTATTAGGACCCTAGATAGGTTGGAAGTTGAGAAAAACGGAGAGGAAATGATTTTTCATGC
CTCTGCTAAGTCGTTTTTGCATCATCAGGTGCGGATAATGGTTGGTACTTTGGTTGCCATTGCATCTGGAAAACTCATGA
GTGTTACAGAAATTCTCTCTAAGAAGAATCGTCGTTACGCTGGCCCGACTGCTCCTCCATGTGGTTTATATCTCCTGAAA
GTGGATTATTAG

Upstream 100 bases:

>100_bases
TCTCAGTAACTGCGTTAACCTAATATATGTTTCCCGTGTGAGGCCGTTTTTCACCTCCTATTCGTGTGTTCATTTTTAAC
GGGTAGTTTTCTAGGTCTTT

Downstream 100 bases:

>100_bases
AGCAAATGCAATCCATACTTGTATAAGTAAGAAAGATGTAGGTATAAATAGACTTCGTTAATCTTCTATGCTAGGATAGA
AGCAGAGTGTATTTTCAGTA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRYKAIVEYVGTGFSGWQKQMSAPSVQEELESVLSFLLKEKIAVNVAGRTDAGVHALGQVFHFDARESTLQPFQIVNAVN
YHLKEKLIVLLKIEIVDETFDARFSAIRRHYQYKITNRKTPLAVFKNRSWHVPQTLNLDLIREQAQYLVGKHDFQSFRSS
KCGASNAIRTLDRLEVEKNGEEMIFHASAKSFLHHQVRIMVGTLVAIASGKLMSVTEILSKKNRRYAGPTAPPCGLYLLK
VDY

Sequences:

>Translated_243_residues
MRYKAIVEYVGTGFSGWQKQMSAPSVQEELESVLSFLLKEKIAVNVAGRTDAGVHALGQVFHFDARESTLQPFQIVNAVN
YHLKEKLIVLLKIEIVDETFDARFSAIRRHYQYKITNRKTPLAVFKNRSWHVPQTLNLDLIREQAQYLVGKHDFQSFRSS
KCGASNAIRTLDRLEVEKNGEEMIFHASAKSFLHHQVRIMVGTLVAIASGKLMSVTEILSKKNRRYAGPTAPPCGLYLLK
VDY
>Mature_243_residues
MRYKAIVEYVGTGFSGWQKQMSAPSVQEELESVLSFLLKEKIAVNVAGRTDAGVHALGQVFHFDARESTLQPFQIVNAVN
YHLKEKLIVLLKIEIVDETFDARFSAIRRHYQYKITNRKTPLAVFKNRSWHVPQTLNLDLIREQAQYLVGKHDFQSFRSS
KCGASNAIRTLDRLEVEKNGEEMIFHASAKSFLHHQVRIMVGTLVAIASGKLMSVTEILSKKNRRYAGPTAPPCGLYLLK
VDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=32.7205882352941, Blast_Score=111, Evalue=6e-25,
Organism=Escherichia coli, GI1788657, Length=239, Percent_Identity=36.8200836820084, Blast_Score=148, Evalue=3e-37,
Organism=Drosophila melanogaster, GI116007752, Length=300, Percent_Identity=29.6666666666667, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_NEOSM (Q2GF33)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_505923.1
- ProteinModelPortal:   Q2GF33
- SMR:   Q2GF33
- STRING:   Q2GF33
- GeneID:   3932291
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0020
- TIGR:   NSE_0020
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   MVIDVRC
- PhylomeDB:   Q2GF33
- ProtClustDB:   CLSK2528006
- BioCyc:   NSEN222891:NSE_0020-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27611; Mature: 27611

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKAIVEYVGTGFSGWQKQMSAPSVQEELESVLSFLLKEKIAVNVAGRTDAGVHALGQV
CCHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHH
FHFDARESTLQPFQIVNAVNYHLKEKLIVLLKIEIVDETFDARFSAIRRHYQYKITNRKT
HCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHEEECCCCC
PLAVFKNRSWHVPQTLNLDLIREQAQYLVGKHDFQSFRSSKCGASNAIRTLDRLEVEKNG
CEEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
EEMIFHASAKSFLHHQVRIMVGTLVAIASGKLMSVTEILSKKNRRYAGPTAPPCGLYLLK
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
VDY
ECC
>Mature Secondary Structure
MRYKAIVEYVGTGFSGWQKQMSAPSVQEELESVLSFLLKEKIAVNVAGRTDAGVHALGQV
CCHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHH
FHFDARESTLQPFQIVNAVNYHLKEKLIVLLKIEIVDETFDARFSAIRRHYQYKITNRKT
HCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHEEECCCCC
PLAVFKNRSWHVPQTLNLDLIREQAQYLVGKHDFQSFRSSKCGASNAIRTLDRLEVEKNG
CEEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
EEMIFHASAKSFLHHQVRIMVGTLVAIASGKLMSVTEILSKKNRRYAGPTAPPCGLYLLK
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
VDY
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA