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The map label for this gene is ligA

Identifier: 88608555

GI number: 88608555

Start: 420546

End: 422558

Strand: Reverse

Name: ligA

Synonym: NSE_0500

Alternate gene names: 88608555

Gene position: 422558-420546 (Counterclockwise)

Preceding gene: 88608470

Following gene: 88608382

Centisome position: 49.19

GC content: 38.7

Gene sequence:

>2013_bases
ATGGAAGCAAAGAAGGAAATTCAAGAGCTTTACGAGAAGCTCCTACGACACAATAAGAAGTACTATCAAGATGATAGTCC
AGAGATAACAGATGCTGAATATGACTCGATTAAGGACCGTTACCTTAAGCTTCTGGAAGCAAATCCGAGTCTAGGCTTTC
CGGTAGTTGTTGGCTACCCTGCTTCTGAGAAGTTTCAAAAAGTCAAACACCTGTCTCCTATGCTTTCATTACGCAACATC
TTCTCGGAAGAAGAACTGGTAGAGTATATCGAAAAAACAAAAAGATTCCTTAATCTGAAAAGTGCACTTGAATTCCTGTG
TGAACCCAAAATAGATGGTGTGTCATTTTCTGCACGTTATGTCAACGGAAAACTAGTCTCTTGTGCAACAAGAGGAGATG
GGAAAATAGGAGAAAACATTATTGATAACATGAAAGTTATCAATGGTTTCCCCATAGAAATTATGGATGTACCAGATCTT
TTGGAAGTACGTGGAGAAGTTTTCCTTGACCATGATACTTTTCAAACTCTTGAAGGCTTTTCAAATCCTCGGAATGCTGC
TGCGGGGTCGCTTAGGCAACTTAATCCTGAAATAACAAATGAACGCAATCTGCAATACTTCGCTTATAGCGCGAGCAAAA
TCGATGGAATAGAACATCAAGAAGATGTGTTACACTTTCTCAGTGGGCGTGGTTTTATGACAAATCCGTTGAGACTAGCT
TCGTCAAAAGTTCCTGAAATCATGAGCTTTTACGATTCTGTTTATCGTAGACGCTCGCAAATCAAGTACGATATAGATGG
GCTGGTCTACAAGGTGAATGATCTCAAGTTACATGCGAGGCTGGGTACACTATCAGATGCACCTAGATGGGCCATAGCGC
ATAAATTTCCCTCACATCGAGCAAAGACTATACTTGAAAAAATTAAGCTCAGCGTTGGGCGTACAGGTATCATCACGCCA
GTTGCTCACTTAAAACCAATTACCATAGGCGGAGTCGTTATATCAAGGGCGAGCTTATATAACGAAGACGAACTAGAGAG
AAAAGATATCCGCGAAGGAGATCTGGTAATAGTAGAAAGAGCTGGAGATGTAATTCCAAAAGTGCTTGAAGTAGACATCT
CGTATAGAACTAATCAGGAGAGGTTTATCTTTCCTGATAAATGTCCATCCTGTTCTTCTACACTGATAAGAAAAAATAAC
GAAGCGGCGACTCGATGCAATAATAGCAAAAAATGCCCCGAACAGGTAATACAACAAATAAAACATCTTGTCTCAGCGCA
AGCGTTCGATATAGATGGTATTGGGACATCACACATCTCTTTTTTTATTGAGAAGGGATTTATTTCAGAACCAGCAGATA
TTTTTCGCTTAGATAAGCACAGAGATGAGATCAAAAAATATGATGGTTGGGGAGAGAAATCAGTAGAGAATATTCTTAAA
AATATTAAAAACTCTCGGAAAATAAGTTTGGAAAAATTTATTTTTAGCCTTGGAATAAAAAATATAGGCGAAAAGACTGC
TTACATGCTTGCACAACAGTTCAAAAGTTTTGCCAACTGGTTTGACAAAATGTCCATGCTCAAAGACGATACTCAAACTG
AGGATGAAATTAGGAATCTCGATGGTATGGGAAGTTGTATCTGTGAATCTTTACTGGATTTTTTCAGTGATTCAGATAAT
TGCAATATGGTAAAAAGTTTATCCAATCACGTCATGATTACTGATCACACTGTTAATATAGGTGGCTCACTTAGCGGGAA
AAAATTCGTTTTTACAGGCACGCTTCTCTCAATTACTAGAGAAGAAGCAAAAGAGATCATAAAAAAAGCCGGTGGTATAG
TTGTGAACAGTATTTCTAAGCAAATCGACTACGTTGTAGCTGGAGAAAAAGCGGGCTCAAAACTTGCAAAAGCCAATGAG
CTTGGTATTGCAATCATAGAAGAAAAAACCCTCATAGAATTCACAAATGGGAACAACACACCTGCTCTTAAGAAAGAGAC
AAGCTCCAACTAA

Upstream 100 bases:

>100_bases
ACTTGAAGTAAAAAAACCGTCTTTTTTTAAACTGCTTGAGTCTGCCTCTGTGCAGGTGGAATATGATAGTGAAACCGATT
CTTAAGAGGCAGCACGTTCA

Downstream 100 bases:

>100_bases
TAATGGATAAAGCGATTTTTTCACCGTACAGTCACTACAAACTTCCTAGAATAGTATTGAATCTCTCAGAGTTATCGTTG
GCAAAAGACTCGACTGATTC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MEAKKEIQELYEKLLRHNKKYYQDDSPEITDAEYDSIKDRYLKLLEANPSLGFPVVVGYPASEKFQKVKHLSPMLSLRNI
FSEEELVEYIEKTKRFLNLKSALEFLCEPKIDGVSFSARYVNGKLVSCATRGDGKIGENIIDNMKVINGFPIEIMDVPDL
LEVRGEVFLDHDTFQTLEGFSNPRNAAAGSLRQLNPEITNERNLQYFAYSASKIDGIEHQEDVLHFLSGRGFMTNPLRLA
SSKVPEIMSFYDSVYRRRSQIKYDIDGLVYKVNDLKLHARLGTLSDAPRWAIAHKFPSHRAKTILEKIKLSVGRTGIITP
VAHLKPITIGGVVISRASLYNEDELERKDIREGDLVIVERAGDVIPKVLEVDISYRTNQERFIFPDKCPSCSSTLIRKNN
EAATRCNNSKKCPEQVIQQIKHLVSAQAFDIDGIGTSHISFFIEKGFISEPADIFRLDKHRDEIKKYDGWGEKSVENILK
NIKNSRKISLEKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDN
CNMVKSLSNHVMITDHTVNIGGSLSGKKFVFTGTLLSITREEAKEIIKKAGGIVVNSISKQIDYVVAGEKAGSKLAKANE
LGIAIIEEKTLIEFTNGNNTPALKKETSSN

Sequences:

>Translated_670_residues
MEAKKEIQELYEKLLRHNKKYYQDDSPEITDAEYDSIKDRYLKLLEANPSLGFPVVVGYPASEKFQKVKHLSPMLSLRNI
FSEEELVEYIEKTKRFLNLKSALEFLCEPKIDGVSFSARYVNGKLVSCATRGDGKIGENIIDNMKVINGFPIEIMDVPDL
LEVRGEVFLDHDTFQTLEGFSNPRNAAAGSLRQLNPEITNERNLQYFAYSASKIDGIEHQEDVLHFLSGRGFMTNPLRLA
SSKVPEIMSFYDSVYRRRSQIKYDIDGLVYKVNDLKLHARLGTLSDAPRWAIAHKFPSHRAKTILEKIKLSVGRTGIITP
VAHLKPITIGGVVISRASLYNEDELERKDIREGDLVIVERAGDVIPKVLEVDISYRTNQERFIFPDKCPSCSSTLIRKNN
EAATRCNNSKKCPEQVIQQIKHLVSAQAFDIDGIGTSHISFFIEKGFISEPADIFRLDKHRDEIKKYDGWGEKSVENILK
NIKNSRKISLEKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDN
CNMVKSLSNHVMITDHTVNIGGSLSGKKFVFTGTLLSITREEAKEIIKKAGGIVVNSISKQIDYVVAGEKAGSKLAKANE
LGIAIIEEKTLIEFTNGNNTPALKKETSSN
>Mature_670_residues
MEAKKEIQELYEKLLRHNKKYYQDDSPEITDAEYDSIKDRYLKLLEANPSLGFPVVVGYPASEKFQKVKHLSPMLSLRNI
FSEEELVEYIEKTKRFLNLKSALEFLCEPKIDGVSFSARYVNGKLVSCATRGDGKIGENIIDNMKVINGFPIEIMDVPDL
LEVRGEVFLDHDTFQTLEGFSNPRNAAAGSLRQLNPEITNERNLQYFAYSASKIDGIEHQEDVLHFLSGRGFMTNPLRLA
SSKVPEIMSFYDSVYRRRSQIKYDIDGLVYKVNDLKLHARLGTLSDAPRWAIAHKFPSHRAKTILEKIKLSVGRTGIITP
VAHLKPITIGGVVISRASLYNEDELERKDIREGDLVIVERAGDVIPKVLEVDISYRTNQERFIFPDKCPSCSSTLIRKNN
EAATRCNNSKKCPEQVIQQIKHLVSAQAFDIDGIGTSHISFFIEKGFISEPADIFRLDKHRDEIKKYDGWGEKSVENILK
NIKNSRKISLEKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDN
CNMVKSLSNHVMITDHTVNIGGSLSGKKFVFTGTLLSITREEAKEIIKKAGGIVVNSISKQIDYVVAGEKAGSKLAKANE
LGIAIIEEKTLIEFTNGNNTPALKKETSSN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=36.3905325443787, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI87082305, Length=441, Percent_Identity=23.1292517006803, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NEOSM (Q2GDR2)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506384.1
- ProteinModelPortal:   Q2GDR2
- STRING:   Q2GDR2
- GeneID:   3931734
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0500
- TIGR:   NSE_0500
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q2GDR2
- ProtClustDB:   CLSK2527991
- BioCyc:   NSEN222891:NSE_0500-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75577; Mature: 75577

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 110-110 BINDING 108-108 BINDING 131-131 BINDING 166-166 BINDING 271-271 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKKEIQELYEKLLRHNKKYYQDDSPEITDAEYDSIKDRYLKLLEANPSLGFPVVVGYP
CCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECC
ASEKFQKVKHLSPMLSLRNIFSEEELVEYIEKTKRFLNLKSALEFLCEPKIDGVSFSARY
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VNGKLVSCATRGDGKIGENIIDNMKVINGFPIEIMDVPDLLEVRGEVFLDHDTFQTLEGF
ECCEEEEEEECCCCCHHHHHHHCHHHHCCCCEEEECCCHHHHCCCCEEECCHHHHHHHCC
SNPRNAAAGSLRQLNPEITNERNLQYFAYSASKIDGIEHQEDVLHFLSGRGFMTNPLRLA
CCCCHHHHCHHHHCCCCCCCCCCCEEEEEECHHCCCCCHHHHHHHHHCCCCCCCCCHHHH
SSKVPEIMSFYDSVYRRRSQIKYDIDGLVYKVNDLKLHARLGTLSDAPRWAIAHKFPSHR
HCCCHHHHHHHHHHHHHHHCCEEECCCEEEEEECEEEEEEECCCCCCCCCHHHCCCCCHH
AKTILEKIKLSVGRTGIITPVAHLKPITIGGVVISRASLYNEDELERKDIREGDLVIVER
HHHHHHHHHHHCCCCCCCCCHHHCCCEEEHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEC
AGDVIPKVLEVDISYRTNQERFIFPDKCPSCSSTLIRKNNEAATRCNNSKKCPEQVIQQI
CCCCCCHHHEEEEEEECCCCCEECCCCCCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHH
KHLVSAQAFDIDGIGTSHISFFIEKGFISEPADIFRLDKHRDEIKKYDGWGEKSVENILK
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NIKNSRKISLEKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNL
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
DGMGSCICESLLDFFSDSDNCNMVKSLSNHVMITDHTVNIGGSLSGKKFVFTGTLLSITR
CCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEHHHHHHHH
EEAKEIIKKAGGIVVNSISKQIDYVVAGEKAGSKLAKANELGIAIIEEKTLIEFTNGNNT
HHHHHHHHHHCCEEEECHHHHCCEEEECCHHCHHHHHHCCCCEEEEECCEEEEEECCCCC
PALKKETSSN
CCCCCCCCCC
>Mature Secondary Structure
MEAKKEIQELYEKLLRHNKKYYQDDSPEITDAEYDSIKDRYLKLLEANPSLGFPVVVGYP
CCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECC
ASEKFQKVKHLSPMLSLRNIFSEEELVEYIEKTKRFLNLKSALEFLCEPKIDGVSFSARY
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VNGKLVSCATRGDGKIGENIIDNMKVINGFPIEIMDVPDLLEVRGEVFLDHDTFQTLEGF
ECCEEEEEEECCCCCHHHHHHHCHHHHCCCCEEEECCCHHHHCCCCEEECCHHHHHHHCC
SNPRNAAAGSLRQLNPEITNERNLQYFAYSASKIDGIEHQEDVLHFLSGRGFMTNPLRLA
CCCCHHHHCHHHHCCCCCCCCCCCEEEEEECHHCCCCCHHHHHHHHHCCCCCCCCCHHHH
SSKVPEIMSFYDSVYRRRSQIKYDIDGLVYKVNDLKLHARLGTLSDAPRWAIAHKFPSHR
HCCCHHHHHHHHHHHHHHHCCEEECCCEEEEEECEEEEEEECCCCCCCCCHHHCCCCCHH
AKTILEKIKLSVGRTGIITPVAHLKPITIGGVVISRASLYNEDELERKDIREGDLVIVER
HHHHHHHHHHHCCCCCCCCCHHHCCCEEEHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEC
AGDVIPKVLEVDISYRTNQERFIFPDKCPSCSSTLIRKNNEAATRCNNSKKCPEQVIQQI
CCCCCCHHHEEEEEEECCCCCEECCCCCCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHH
KHLVSAQAFDIDGIGTSHISFFIEKGFISEPADIFRLDKHRDEIKKYDGWGEKSVENILK
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NIKNSRKISLEKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNL
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
DGMGSCICESLLDFFSDSDNCNMVKSLSNHVMITDHTVNIGGSLSGKKFVFTGTLLSITR
CCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEHHHHHHHH
EEAKEIIKKAGGIVVNSISKQIDYVVAGEKAGSKLAKANELGIAIIEEKTLIEFTNGNNT
HHHHHHHHHHCCEEEECHHHHCCEEEECCHHCHHHHHHCCCCEEEEECCEEEEEECCCCC
PALKKETSSN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA