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The map label for this gene is gshA

Identifier: 88608518

GI number: 88608518

Start: 251072

End: 252247

Strand: Reverse

Name: gshA

Synonym: NSE_0308

Alternate gene names: NA

Gene position: 252247-251072 (Counterclockwise)

Preceding gene: 88608331

Following gene: 88608399

Centisome position: 29.36

GC content: 39.03

Gene sequence:

>1176_bases
ATGGATAGACATTTCTCAAAGATTTCTACGAATATAGGAGAAATCCGTCGGTGGTGCCACAGCAGAATTAGTGGAAAGTT
GTTGTACCTCTCGGCAGACATAAGGAACGCTAGATTTAAACTTGCCCCGGTAGATGTAAACATCTTTCCAGCTGGATTCC
ACTTACTCAATAAGGAAAACCAGACACTCGCAACAAGGTTGATTAGTGAACGCTATACAGGAAAGACAATTTTACTAATC
ATAGAGACACATACCAGAAATACTCACTATTTAAGCAACGTTCACACACTGAAAAAATTATTTGAAGATGCAAAAAATAC
CGTTATTCTAGGAAATCCTAGCGACCAGAAAATAGAAAATATAAATACAGAAACTTTTCATATAAAGGATAAAAGAATCA
TCACGGAAAGTGGCACGACTCCGGATTTAATAGTACTAAACAACGACATGATCTCACTCGACCCTTCTGTCCTCCTAGAA
GTAACCCAACCGTGCCTCCCTTCCCCATTACTAGGTTGGTGGAAAAGGACAAAAACTCAGTATTTCCCATACTATGAAAA
AACGGTAGAGGAATTCTCCAATACTTTTGAGTTAGATCCGTGGTTATTCTATGCTTATTCAGAAGCTTGTGACAATATTA
ACTTTGACACTCAAGAAAGTCTCGCAAGGCTTGCAGATGTTGTAGATGGAATAATTCAAAAGATCCAGAAAAACTACAAG
CAGTATGGAATATCGTCAAAACCGTTTGTTTTTATCAAAGCAAACAGCGGTAGTTATGGTATGGGGATCCTCACTGCAAG
CAGTGGAAAAGAAATTCTCTCTATGAACAGAAAAGAGCGTAAAAAATTAGGAACCACTAAAGGTAATACTGCAATTACAA
GTGTTATAGTCCAGGAAGGCATAGAAACGGAAGAATATTATCTAAACTACCCTGCAGAGACCCTTATGTACTCGGTTCTA
GGGAAAAAAATTGCATTCGCAACACGCTATAATACGAAACATGATGCGCGACAAAACCTAAACAGCTCTGGAATGCATTT
TTCTGGCCACACGCCTATGAAAGCAAGTCAAGTGCAACTAGAAATTGTAAGTGATCTTATCATTATCGCAGCAGAAAAGG
AGGCCCAATCCATAGACGATAAAAACGTCGCCGCACTACCGCATCAAGCTGGTTAA

Upstream 100 bases:

>100_bases
TGTTGTTTTACACGCGTGAAAATCCATGTTAGAATCACCCCTTCCCCTTTTACGCAAACGCTTCCTAACGTAGTAAGTCT
CTAAAGGAGCGCTCTTCTCA

Downstream 100 bases:

>100_bases
TTTCATCTGAAATTGCTCGATGTGGAAAAAGAATAACGGACCGCTATTAGTACCCATACCGACCTGTTACGGAAGACCAT
ATAACACTACTTCTCGGGAT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MDRHFSKISTNIGEIRRWCHSRISGKLLYLSADIRNARFKLAPVDVNIFPAGFHLLNKENQTLATRLISERYTGKTILLI
IETHTRNTHYLSNVHTLKKLFEDAKNTVILGNPSDQKIENINTETFHIKDKRIITESGTTPDLIVLNNDMISLDPSVLLE
VTQPCLPSPLLGWWKRTKTQYFPYYEKTVEEFSNTFELDPWLFYAYSEACDNINFDTQESLARLADVVDGIIQKIQKNYK
QYGISSKPFVFIKANSGSYGMGILTASSGKEILSMNRKERKKLGTTKGNTAITSVIVQEGIETEEYYLNYPAETLMYSVL
GKKIAFATRYNTKHDARQNLNSSGMHFSGHTPMKASQVQLEIVSDLIIIAAEKEAQSIDDKNVAALPHQAG

Sequences:

>Translated_391_residues
MDRHFSKISTNIGEIRRWCHSRISGKLLYLSADIRNARFKLAPVDVNIFPAGFHLLNKENQTLATRLISERYTGKTILLI
IETHTRNTHYLSNVHTLKKLFEDAKNTVILGNPSDQKIENINTETFHIKDKRIITESGTTPDLIVLNNDMISLDPSVLLE
VTQPCLPSPLLGWWKRTKTQYFPYYEKTVEEFSNTFELDPWLFYAYSEACDNINFDTQESLARLADVVDGIIQKIQKNYK
QYGISSKPFVFIKANSGSYGMGILTASSGKEILSMNRKERKKLGTTKGNTAITSVIVQEGIETEEYYLNYPAETLMYSVL
GKKIAFATRYNTKHDARQNLNSSGMHFSGHTPMKASQVQLEIVSDLIIIAAEKEAQSIDDKNVAALPHQAG
>Mature_391_residues
MDRHFSKISTNIGEIRRWCHSRISGKLLYLSADIRNARFKLAPVDVNIFPAGFHLLNKENQTLATRLISERYTGKTILLI
IETHTRNTHYLSNVHTLKKLFEDAKNTVILGNPSDQKIENINTETFHIKDKRIITESGTTPDLIVLNNDMISLDPSVLLE
VTQPCLPSPLLGWWKRTKTQYFPYYEKTVEEFSNTFELDPWLFYAYSEACDNINFDTQESLARLADVVDGIIQKIQKNYK
QYGISSKPFVFIKANSGSYGMGILTASSGKEILSMNRKERKKLGTTKGNTAITSVIVQEGIETEEYYLNYPAETLMYSVL
GKKIAFATRYNTKHDARQNLNSSGMHFSGHTPMKASQVQLEIVSDLIIIAAEKEAQSIDDKNVAALPHQAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44281; Mature: 44281

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRHFSKISTNIGEIRRWCHSRISGKLLYLSADIRNARFKLAPVDVNIFPAGFHLLNKEN
CCCCHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEEEEEECCCEEEECCCC
QTLATRLISERYTGKTILLIIETHTRNTHYLSNVHTLKKLFEDAKNTVILGNPSDQKIEN
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHC
INTETFHIKDKRIITESGTTPDLIVLNNDMISLDPSVLLEVTQPCLPSPLLGWWKRTKTQ
CCCEEEEEECCEEEECCCCCCEEEEEECCEEECCHHHHHHHHCCCCCCHHHHHHHHCCCC
YFPYYEKTVEEFSNTFELDPWLFYAYSEACDNINFDTQESLARLADVVDGIIQKIQKNYK
CCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QYGISSKPFVFIKANSGSYGMGILTASSGKEILSMNRKERKKLGTTKGNTAITSVIVQEG
HHCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCC
IETEEYYLNYPAETLMYSVLGKKIAFATRYNTKHDARQNLNSSGMHFSGHTPMKASQVQL
CCCCCEEECCCHHHHHHHHHHHHHEEEEECCCCHHHHHCCCCCCCEECCCCCCCHHHHHH
EIVSDLIIIAAEKEAQSIDDKNVAALPHQAG
EECCCEEEEEECHHHHCCCCCCEECCCCCCC
>Mature Secondary Structure
MDRHFSKISTNIGEIRRWCHSRISGKLLYLSADIRNARFKLAPVDVNIFPAGFHLLNKEN
CCCCHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEEEEEECCCEEEECCCC
QTLATRLISERYTGKTILLIIETHTRNTHYLSNVHTLKKLFEDAKNTVILGNPSDQKIEN
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHC
INTETFHIKDKRIITESGTTPDLIVLNNDMISLDPSVLLEVTQPCLPSPLLGWWKRTKTQ
CCCEEEEEECCEEEECCCCCCEEEEEECCEEECCHHHHHHHHCCCCCCHHHHHHHHCCCC
YFPYYEKTVEEFSNTFELDPWLFYAYSEACDNINFDTQESLARLADVVDGIIQKIQKNYK
CCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QYGISSKPFVFIKANSGSYGMGILTASSGKEILSMNRKERKKLGTTKGNTAITSVIVQEG
HHCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCC
IETEEYYLNYPAETLMYSVLGKKIAFATRYNTKHDARQNLNSSGMHFSGHTPMKASQVQL
CCCCCEEECCCHHHHHHHHHHHHHEEEEECCCCHHHHHCCCCCCCEECCCCCCCHHHHHH
EIVSDLIIIAAEKEAQSIDDKNVAALPHQAG
EECCCEEEEEECHHHHCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA