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The map label for this gene is ubiE

Identifier: 88608508

GI number: 88608508

Start: 836412

End: 837104

Strand: Direct

Name: ubiE

Synonym: NSE_0950

Alternate gene names: 88608508

Gene position: 836412-837104 (Clockwise)

Preceding gene: 88608736

Following gene: 88608379

Centisome position: 97.37

GC content: 40.55

Gene sequence:

>693_bases
ATGGAAAAGGACTTTGTAAATGAAATATTCTCCTCAGTGTCACAGAAATATGATCTGATGAATGACATAATGAGTGGGGG
CATACACAGACTCTGGAAACGCAACTTAGTGAAGTCGATTGGCTTACTTCCGAATCAACGTGTACTTGATATGGCTGCGG
GAACAGGAGATATCACGCTACGTCTTTTAAAGACACACATTCCAGTAGAAATAATCCTATGTGATAAAAATCATGAGATG
TTGGAAATAGCAAAGGACCGCCTACTTGATGAAGGTTATGTGAACTTAAAGGTAGTCTCAGCTGATGCAGCACAGTTACC
ATTCGAAGATTGCAGCTTCGATCATTACGTCGTTGCATTTGGTGTGCGCAATTTTTCCAATATTGAGAGATCTCTAACAG
AAGCTTACAGAGTCTTGAAACCAGGCGGAAGGTTCTCCTGTTTAGAGTTCAGCAAGGTAAAAAACAAGTGTATGAATTCG
CTGTATAATCTTTATTCACACACCTTCATTCCATGGGTGGGAGAGAAGATAACTAAGAATAGGCAAGCTTATACATACCT
AATCGAAAGTATACAAGACTTTCCTGACGCGGAGACCTTTTGTGAAATTATTCGTAGTGCGGGATTCGGAAGAACTAAAT
ACAGAAGGGAAACGTTTGGGGTAGCAGCGATTCACACAGCGACAAAACTATGA

Upstream 100 bases:

>100_bases
CCCACCAACAGTGGAAAGATAAGAAACATAAGAAGGCGCCTTGGAAATACCTTTAGGAGTGCTTGGGTTTTCACGCTCCC
AGGTTTTGATTTAAATAGAA

Downstream 100 bases:

>100_bases
TGTTTAAGTTAAAGTTTCCTGAATATAATTGCGAGGTAGCTTCAGTACAAACAAGCAAGAGGAGCTCGATAAAATTATCG
CACAATGCAGGTGCTTATCT

Product: ubiquinone/menaquinone biosynthesis methlytransferase UbiE

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MEKDFVNEIFSSVSQKYDLMNDIMSGGIHRLWKRNLVKSIGLLPNQRVLDMAAGTGDITLRLLKTHIPVEIILCDKNHEM
LEIAKDRLLDEGYVNLKVVSADAAQLPFEDCSFDHYVVAFGVRNFSNIERSLTEAYRVLKPGGRFSCLEFSKVKNKCMNS
LYNLYSHTFIPWVGEKITKNRQAYTYLIESIQDFPDAETFCEIIRSAGFGRTKYRRETFGVAAIHTATKL

Sequences:

>Translated_230_residues
MEKDFVNEIFSSVSQKYDLMNDIMSGGIHRLWKRNLVKSIGLLPNQRVLDMAAGTGDITLRLLKTHIPVEIILCDKNHEM
LEIAKDRLLDEGYVNLKVVSADAAQLPFEDCSFDHYVVAFGVRNFSNIERSLTEAYRVLKPGGRFSCLEFSKVKNKCMNS
LYNLYSHTFIPWVGEKITKNRQAYTYLIESIQDFPDAETFCEIIRSAGFGRTKYRRETFGVAAIHTATKL
>Mature_230_residues
MEKDFVNEIFSSVSQKYDLMNDIMSGGIHRLWKRNLVKSIGLLPNQRVLDMAAGTGDITLRLLKTHIPVEIILCDKNHEM
LEIAKDRLLDEGYVNLKVVSADAAQLPFEDCSFDHYVVAFGVRNFSNIERSLTEAYRVLKPGGRFSCLEFSKVKNKCMNS
LYNLYSHTFIPWVGEKITKNRQAYTYLIESIQDFPDAETFCEIIRSAGFGRTKYRRETFGVAAIHTATKL

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=260, Percent_Identity=42.3076923076923, Blast_Score=211, Evalue=5e-55,
Organism=Escherichia coli, GI48994983, Length=231, Percent_Identity=39.8268398268398, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17557039, Length=227, Percent_Identity=42.2907488986784, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323526, Length=248, Percent_Identity=39.5161290322581, Blast_Score=172, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24641702, Length=240, Percent_Identity=44.5833333333333, Blast_Score=210, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26318; Mature: 26318

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDFVNEIFSSVSQKYDLMNDIMSGGIHRLWKRNLVKSIGLLPNQRVLDMAAGTGDITL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHH
RLLKTHIPVEIILCDKNHEMLEIAKDRLLDEGYVNLKVVSADAAQLPFEDCSFDHYVVAF
HHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCCEEEEE
GVRNFSNIERSLTEAYRVLKPGGRFSCLEFSKVKNKCMNSLYNLYSHTFIPWVGEKITKN
ECHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
RQAYTYLIESIQDFPDAETFCEIIRSAGFGRTKYRRETFGVAAIHTATKL
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure
MEKDFVNEIFSSVSQKYDLMNDIMSGGIHRLWKRNLVKSIGLLPNQRVLDMAAGTGDITL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHH
RLLKTHIPVEIILCDKNHEMLEIAKDRLLDEGYVNLKVVSADAAQLPFEDCSFDHYVVAF
HHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCCEEEEE
GVRNFSNIERSLTEAYRVLKPGGRFSCLEFSKVKNKCMNSLYNLYSHTFIPWVGEKITKN
ECHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
RQAYTYLIESIQDFPDAETFCEIIRSAGFGRTKYRRETFGVAAIHTATKL
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA