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The map label for this gene is smpB

Identifier: 88608497

GI number: 88608497

Start: 719542

End: 719985

Strand: Reverse

Name: smpB

Synonym: NSE_0811

Alternate gene names: 88608497

Gene position: 719985-719542 (Counterclockwise)

Preceding gene: 88608184

Following gene: 88608315

Centisome position: 83.82

GC content: 38.51

Gene sequence:

>444_bases
ATGAAAGTCATTGCAATCAATAAAAAGGCCCGATTTGATTACTCACTAATTGATGAATTTGAAGCCGGGATTGTCTTACT
TGGAACGGAAGTTAAGGCTTTAAAGTATCATTCAGCTTCATTAAACGAGGCCTTTGTTGCCTTTAGGCGTTTTGAACTGT
GGCTTAATAATATGCATGTTCCACCCTATAAGCCTGCAAGTAGGTTCAATCACGTTCCCACTAGATCTCGGAAATTACTT
GTTCACAAAAGACAGCTAAATAAAATATCTGGCGCAGTAAGAACCAAAGGACTAACTCTAGTTCCATTATCTATATACGT
AAACGAAAATGGGCTTATAAAAGTGAAGTTTGCCTTAGCAAAAGGTAAGAAAAAACATGATAAACGTCAGGACATCAAGG
ATCGTGATTGGGCAAGAAAGCAAGCCCGGCAAGATTTCAGCTAG

Upstream 100 bases:

>100_bases
AGCTTTGTAGGGATCTTGTCTTGCTGATAACGTAAAAATGTTCTCACTTTCTCACCAAGTAATAGAGTGTTAGGATCTAC
GTGCAGGTAGCGTCCACAGA

Downstream 100 bases:

>100_bases
GTGTGCATGCATCTATCTGTTTGTGAAGCAATTAGTTTGCTTTGTATTGCTACAACTCTATGCGCAGGTGAATCAGTTTC
TCCTGGGTATCCGAGGCTTC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKVIAINKKARFDYSLIDEFEAGIVLLGTEVKALKYHSASLNEAFVAFRRFELWLNNMHVPPYKPASRFNHVPTRSRKLL
VHKRQLNKISGAVRTKGLTLVPLSIYVNENGLIKVKFALAKGKKKHDKRQDIKDRDWARKQARQDFS

Sequences:

>Translated_147_residues
MKVIAINKKARFDYSLIDEFEAGIVLLGTEVKALKYHSASLNEAFVAFRRFELWLNNMHVPPYKPASRFNHVPTRSRKLL
VHKRQLNKISGAVRTKGLTLVPLSIYVNENGLIKVKFALAKGKKKHDKRQDIKDRDWARKQARQDFS
>Mature_147_residues
MKVIAINKKARFDYSLIDEFEAGIVLLGTEVKALKYHSASLNEAFVAFRRFELWLNNMHVPPYKPASRFNHVPTRSRKLL
VHKRQLNKISGAVRTKGLTLVPLSIYVNENGLIKVKFALAKGKKKHDKRQDIKDRDWARKQARQDFS

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=45.1388888888889, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_NEOSM (Q2GCW3)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506683.1
- ProteinModelPortal:   Q2GCW3
- SMR:   Q2GCW3
- STRING:   Q2GCW3
- GeneID:   3931343
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0811
- TIGR:   NSE_0811
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q2GCW3
- ProtClustDB:   PRK05422
- BioCyc:   NSEN222891:NSE_0811-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17030; Mature: 17030

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIAINKKARFDYSLIDEFEAGIVLLGTEVKALKYHSASLNEAFVAFRRFELWLNNMHV
CEEEEECCCCCCCHHHHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
PPYKPASRFNHVPTRSRKLLVHKRQLNKISGAVRTKGLTLVPLSIYVNENGLIKVKFALA
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEEEC
KGKKKHDKRQDIKDRDWARKQARQDFS
CCCCCCHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVIAINKKARFDYSLIDEFEAGIVLLGTEVKALKYHSASLNEAFVAFRRFELWLNNMHV
CEEEEECCCCCCCHHHHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
PPYKPASRFNHVPTRSRKLLVHKRQLNKISGAVRTKGLTLVPLSIYVNENGLIKVKFALA
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEEEC
KGKKKHDKRQDIKDRDWARKQARQDFS
CCCCCCHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA