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The map label for this gene is glnA

Identifier: 88608432

GI number: 88608432

Start: 288531

End: 289937

Strand: Reverse

Name: glnA

Synonym: NSE_0350

Alternate gene names: 88608432

Gene position: 289937-288531 (Counterclockwise)

Preceding gene: 88608226

Following gene: 88608599

Centisome position: 33.75

GC content: 44.42

Gene sequence:

>1407_bases
GTGACAAATAACAGGGCTGCTTCCATACTCGAGTTCGTTGCCAAAAACGAGATTAGGTTTATTGATTTCAGATTTACCTC
GATCACCGGGAAATTTTTACATATCACGCGTCATAGCGATAGCATCACTGCAGATGCTCTCACGCATGGTATTTCGCTCG
ACAGCTCATTGATTCAAGGCTGGCATGATGTGGATAGATCCGATGTAGTGCTCGTACCGGATCTCGAGTCGTGTAGTTCT
TTCTTGGATCCATTCGCCGCACAGCCGACATTGTGCGTTGTTTGTGACATTGTTGAGCCGCACGGACGTGGTTCGTATGA
GCATAGTCCACGCGCAACAACGAAGAAAGCTATGGAATTTTTACGCAGCATTGGAATAGCTGACAAGGCTTTTTTTGGTC
TCGAGATGGAGTTTTTCGTACTGGATGATGCTAGGTTTGCCGTCAAAGAAAATGGAGCTTTCTTTGAACTGGATTCATCA
GAAGGGATCTATAACTCTGGCAGACGGTATGAATATAATAATCACGCTCACAGAGAAGGCAGCAGAAACTGCTATCTGGC
AAGTCAACCTTCGGATTCTCTAAATGACATAAGGGCAGAGATTCTAGAAACACTCAGAGAAGTAAAAATAAAACCATTAC
TGCACCATCACGGAGCTGCATCGTCCCAGTGCAGAATATGCTTCCACCATGGGGAGGCTTTAGAGACTTCTGATAATATG
CAAAAGTGTAAGTACATCACACGCAACGTTGCCAGTTCTTATGGAAAGTCGGCTACTTTCATGCCTAAGCCATTCAAGGA
CTACAACGGCAATGGAATGCGCTTACACGTATCGCTCTGGAAGGATGAGCAAAATTTGTTCGTACCACAAGATCCAAGAG
AGGAGATTTCACAGTTGTGTAAATACTACATTGGTGGAATCATAAAGCATAGGAAAGCGCTGAACGCTATTTTGAACCCG
CTTGCTAATAGCTACAAGCGCCTCGCAGATATCCATAATGCATCTTACGGGCTAGGTTATTCGCCTGAAAATCGTCTAGC
AGTGGTAAGGATTCCACACTGCAGGAAGGGTAATCCTTCCGGAAAAAGAATAGAGATTAGGTTTCCTGATGCGGGTACTA
ACCCCTATATCGGAACCGCCGCTTTGATTATGGCAGGTATAGCGGGTATACGGGAAAAAGTACTACCGGAAGCTGCTGTG
GAACTCGAAAAGTCTTCAAAAGTTTCTTTGTGTAGGTCTTTGGAGGAGGCACTCCTCGCACTCGACACTGATAGACAATT
CCTGACAGAAGGTGATGTGTTCACGAATGATCAGCTTGATACCTATATAAAGCTCCTAAGTGATGACTCTGAGGAGGTAT
CAAAACATCCCGTACCGATTGAATTTATCAAATACTATAACGTTTAG

Upstream 100 bases:

>100_bases
TACTTTTCTCATGCAAAAAAGCACCTTAGAAGAAACCCTAAAACAGATTGCACAATCAATGAACGGAGAATACAATTATC
TTGGTTTCTTTGCTACACAA

Downstream 100 bases:

>100_bases
GGTCGTATGAGTATTGGCACCGATTGGGTGGAGGCGCTTCATATCATTGTTGTGACAATGTGGATGGCAGGCCTTTTTTA
CTTACCCAGAATCATGGTCT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTNNRAASILEFVAKNEIRFIDFRFTSITGKFLHITRHSDSITADALTHGISLDSSLIQGWHDVDRSDVVLVPDLESCSS
FLDPFAAQPTLCVVCDIVEPHGRGSYEHSPRATTKKAMEFLRSIGIADKAFFGLEMEFFVLDDARFAVKENGAFFELDSS
EGIYNSGRRYEYNNHAHREGSRNCYLASQPSDSLNDIRAEILETLREVKIKPLLHHHGAASSQCRICFHHGEALETSDNM
QKCKYITRNVASSYGKSATFMPKPFKDYNGNGMRLHVSLWKDEQNLFVPQDPREEISQLCKYYIGGIIKHRKALNAILNP
LANSYKRLADIHNASYGLGYSPENRLAVVRIPHCRKGNPSGKRIEIRFPDAGTNPYIGTAALIMAGIAGIREKVLPEAAV
ELEKSSKVSLCRSLEEALLALDTDRQFLTEGDVFTNDQLDTYIKLLSDDSEEVSKHPVPIEFIKYYNV

Sequences:

>Translated_468_residues
MTNNRAASILEFVAKNEIRFIDFRFTSITGKFLHITRHSDSITADALTHGISLDSSLIQGWHDVDRSDVVLVPDLESCSS
FLDPFAAQPTLCVVCDIVEPHGRGSYEHSPRATTKKAMEFLRSIGIADKAFFGLEMEFFVLDDARFAVKENGAFFELDSS
EGIYNSGRRYEYNNHAHREGSRNCYLASQPSDSLNDIRAEILETLREVKIKPLLHHHGAASSQCRICFHHGEALETSDNM
QKCKYITRNVASSYGKSATFMPKPFKDYNGNGMRLHVSLWKDEQNLFVPQDPREEISQLCKYYIGGIIKHRKALNAILNP
LANSYKRLADIHNASYGLGYSPENRLAVVRIPHCRKGNPSGKRIEIRFPDAGTNPYIGTAALIMAGIAGIREKVLPEAAV
ELEKSSKVSLCRSLEEALLALDTDRQFLTEGDVFTNDQLDTYIKLLSDDSEEVSKHPVPIEFIKYYNV
>Mature_467_residues
TNNRAASILEFVAKNEIRFIDFRFTSITGKFLHITRHSDSITADALTHGISLDSSLIQGWHDVDRSDVVLVPDLESCSSF
LDPFAAQPTLCVVCDIVEPHGRGSYEHSPRATTKKAMEFLRSIGIADKAFFGLEMEFFVLDDARFAVKENGAFFELDSSE
GIYNSGRRYEYNNHAHREGSRNCYLASQPSDSLNDIRAEILETLREVKIKPLLHHHGAASSQCRICFHHGEALETSDNMQ
KCKYITRNVASSYGKSATFMPKPFKDYNGNGMRLHVSLWKDEQNLFVPQDPREEISQLCKYYIGGIIKHRKALNAILNPL
ANSYKRLADIHNASYGLGYSPENRLAVVRIPHCRKGNPSGKRIEIRFPDAGTNPYIGTAALIMAGIAGIREKVLPEAAVE
LEKSSKVSLCRSLEEALLALDTDRQFLTEGDVFTNDQLDTYIKLLSDDSEEVSKHPVPIEFIKYYNV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=472, Percent_Identity=40.6779661016949, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI87081870, Length=389, Percent_Identity=26.2210796915167, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52618; Mature: 52487

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNRAASILEFVAKNEIRFIDFRFTSITGKFLHITRHSDSITADALTHGISLDSSLIQG
CCCCHHHHHHHHHHCCCEEEEEEEEEEECCEEEEEEECCCCCCHHHHHHCCCCCHHHHHH
WHDVDRSDVVLVPDLESCSSFLDPFAAQPTLCVVCDIVEPHGRGSYEHSPRATTKKAMEF
HHCCCCCCEEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
LRSIGIADKAFFGLEMEFFVLDDARFAVKENGAFFELDSSEGIYNSGRRYEYNNHAHREG
HHHCCCCCHHEECCEEEEEEECCCEEEEECCCCEEEECCCCCCCCCCCCEECCCCCCCCC
SRNCYLASQPSDSLNDIRAEILETLREVKIKPLLHHHGAASSQCRICFHHGEALETSDNM
CCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCHH
QKCKYITRNVASSYGKSATFMPKPFKDYNGNGMRLHVSLWKDEQNLFVPQDPREEISQLC
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
KYYIGGIIKHRKALNAILNPLANSYKRLADIHNASYGLGYSPENRLAVVRIPHCRKGNPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GKRIEIRFPDAGTNPYIGTAALIMAGIAGIREKVLPEAAVELEKSSKVSLCRSLEEALLA
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHH
LDTDRQFLTEGDVFTNDQLDTYIKLLSDDSEEVSKHPVPIEFIKYYNV
HCCCHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCEEHHEECCC
>Mature Secondary Structure 
TNNRAASILEFVAKNEIRFIDFRFTSITGKFLHITRHSDSITADALTHGISLDSSLIQG
CCCHHHHHHHHHHCCCEEEEEEEEEEECCEEEEEEECCCCCCHHHHHHCCCCCHHHHHH
WHDVDRSDVVLVPDLESCSSFLDPFAAQPTLCVVCDIVEPHGRGSYEHSPRATTKKAMEF
HHCCCCCCEEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
LRSIGIADKAFFGLEMEFFVLDDARFAVKENGAFFELDSSEGIYNSGRRYEYNNHAHREG
HHHCCCCCHHEECCEEEEEEECCCEEEEECCCCEEEECCCCCCCCCCCCEECCCCCCCCC
SRNCYLASQPSDSLNDIRAEILETLREVKIKPLLHHHGAASSQCRICFHHGEALETSDNM
CCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCHH
QKCKYITRNVASSYGKSATFMPKPFKDYNGNGMRLHVSLWKDEQNLFVPQDPREEISQLC
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
KYYIGGIIKHRKALNAILNPLANSYKRLADIHNASYGLGYSPENRLAVVRIPHCRKGNPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GKRIEIRFPDAGTNPYIGTAALIMAGIAGIREKVLPEAAVELEKSSKVSLCRSLEEALLA
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHH
LDTDRQFLTEGDVFTNDQLDTYIKLLSDDSEEVSKHPVPIEFIKYYNV
HCCCHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2878685 [H]