Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 88608395

GI number: 88608395

Start: 400180

End: 400950

Strand: Direct

Name: thiG

Synonym: NSE_0483

Alternate gene names: 88608395

Gene position: 400180-400950 (Clockwise)

Preceding gene: 88608310

Following gene: 88608257

Centisome position: 46.59

GC content: 43.45

Gene sequence:

>771_bases
ATGCTCGATCATTTAGAAATCGCGGGAAGAAATTTCTCCTCTCGTTTGATTGTTGGTACTGGTAAATACAAGAATTTCGA
TGAGACTGTTAAAGCCATAGAAGCCTCCGGTGCTGAGGTGGTCACAGTTGCTCTAAAAAGGGTGAACATTACCGATAATA
AACGTGAAAGTTTGCAGGATTATCTCGATCCTAAGAAATATACTTACCTTCCTAATACTGCGTTCTGTTTTACTGCGGGA
GAAGCAGTTCGACATCTAGCTCTAGCAAGAGAAATAGGCGGCTGGAACCTTGTTAAAGTAGAGGTTTTTTCAGAGAAGGA
GTTTCTTTACCCAGACATGCGGGAAACGTTAAAAGCAGTCAAAATTTTGGCTGGAGAAGGATTTCACGTTATGCCGTACT
GCAATGATGATCCCATAATGTGTAAAAGGCTAGAAGAGAGCGGTGCGGTTGCGATTATGCCACTTGCAGCGCCGATAGGT
TCAGGTTTGGGTATACAGAATCTTTTTAATTTGAAAGTTATTGTGAAGCAATCTAGTGTTCCGGTAATAGTGGATGCTGG
AGTTGGTACGCCGTCGGACGCTGTAATAGCGATGGAAGCCGGTTGCGATGGGGTGCTTATAAATACTTCAATAGCAAAAG
CGCGTTTCCCTGTTCAAATGGCAAAGGCGATGAAACATGCTGTTCGTGCTGGTAGATTAGGCTACTTAGCTGGCAGGATG
CTACCTCAACAGTTTGCTTCTCCTTCCTCAAATAGTTCCGGTATTGTTTGA

Upstream 100 bases:

>100_bases
TCCTCTGTCTTCTAGGTAGTAGGGTTTTGAAAAGCTTGTCTTGGATCCTGTTTGGCTGGTAATCTTTTTAAGTCGCTAAT
GATTTGAAATCCTGTTATTT

Downstream 100 bases:

>100_bases
ATTTATCGTTAGATATCTTCTTTATCTGGAAAAGAATTTTAGGCCCTTTAGTTTTCCGCTGATCCTCTAGTATGTGTATA
CGTTACCGTAGGCATCTTCT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLDHLEIAGRNFSSRLIVGTGKYKNFDETVKAIEASGAEVVTVALKRVNITDNKRESLQDYLDPKKYTYLPNTAFCFTAG
EAVRHLALAREIGGWNLVKVEVFSEKEFLYPDMRETLKAVKILAGEGFHVMPYCNDDPIMCKRLEESGAVAIMPLAAPIG
SGLGIQNLFNLKVIVKQSSVPVIVDAGVGTPSDAVIAMEAGCDGVLINTSIAKARFPVQMAKAMKHAVRAGRLGYLAGRM
LPQQFASPSSNSSGIV

Sequences:

>Translated_256_residues
MLDHLEIAGRNFSSRLIVGTGKYKNFDETVKAIEASGAEVVTVALKRVNITDNKRESLQDYLDPKKYTYLPNTAFCFTAG
EAVRHLALAREIGGWNLVKVEVFSEKEFLYPDMRETLKAVKILAGEGFHVMPYCNDDPIMCKRLEESGAVAIMPLAAPIG
SGLGIQNLFNLKVIVKQSSVPVIVDAGVGTPSDAVIAMEAGCDGVLINTSIAKARFPVQMAKAMKHAVRAGRLGYLAGRM
LPQQFASPSSNSSGIV
>Mature_256_residues
MLDHLEIAGRNFSSRLIVGTGKYKNFDETVKAIEASGAEVVTVALKRVNITDNKRESLQDYLDPKKYTYLPNTAFCFTAG
EAVRHLALAREIGGWNLVKVEVFSEKEFLYPDMRETLKAVKILAGEGFHVMPYCNDDPIMCKRLEESGAVAIMPLAAPIG
SGLGIQNLFNLKVIVKQSSVPVIVDAGVGTPSDAVIAMEAGCDGVLINTSIAKARFPVQMAKAMKHAVRAGRLGYLAGRM
LPQQFASPSSNSSGIV

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=45.6349206349206, Blast_Score=214, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_NEOSM (Q2GDS9)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506367.1
- ProteinModelPortal:   Q2GDS9
- SMR:   Q2GDS9
- STRING:   Q2GDS9
- GeneID:   3932299
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0483
- TIGR:   NSE_0483
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q2GDS9
- ProtClustDB:   PRK00208
- BioCyc:   NSEN222891:NSE_0483-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27677; Mature: 27677

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: ACT_SITE 99-99 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDHLEIAGRNFSSRLIVGTGKYKNFDETVKAIEASGAEVVTVALKRVNITDNKRESLQD
CCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHH
YLDPKKYTYLPNTAFCFTAGEAVRHLALAREIGGWNLVKVEVFSEKEFLYPDMRETLKAV
HCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHH
KILAGEGFHVMPYCNDDPIMCKRLEESGAVAIMPLAAPIGSGLGIQNLFNLKVIVKQSSV
HHHCCCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHEEEEEEEECCCC
PVIVDAGVGTPSDAVIAMEAGCDGVLINTSIAKARFPVQMAKAMKHAVRAGRLGYLAGRM
CEEEECCCCCCCCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHCHHHHHHHHH
LPQQFASPSSNSSGIV
CHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDHLEIAGRNFSSRLIVGTGKYKNFDETVKAIEASGAEVVTVALKRVNITDNKRESLQD
CCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHH
YLDPKKYTYLPNTAFCFTAGEAVRHLALAREIGGWNLVKVEVFSEKEFLYPDMRETLKAV
HCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHH
KILAGEGFHVMPYCNDDPIMCKRLEESGAVAIMPLAAPIGSGLGIQNLFNLKVIVKQSSV
HHHCCCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHEEEEEEEECCCC
PVIVDAGVGTPSDAVIAMEAGCDGVLINTSIAKARFPVQMAKAMKHAVRAGRLGYLAGRM
CEEEECCCCCCCCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHCHHHHHHHHH
LPQQFASPSSNSSGIV
CHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA