Click here to switch to the map view.

The map label for this gene is folE

Identifier: 88608376

GI number: 88608376

Start: 509055

End: 509597

Strand: Reverse

Name: folE

Synonym: NSE_0587

Alternate gene names: 88608376

Gene position: 509597-509055 (Counterclockwise)

Preceding gene: 88608572

Following gene: 88608362

Centisome position: 59.32

GC content: 42.91

Gene sequence:

>543_bases
GTGCAGAAGTTATCTGAGGGCGATATTCAGGATTTTTTCGGTGTCGGGGTAGGTGAGACTGATCGCCTAGGGGCACGTGG
TGCGTCACACTCATTTCTCAAGATGTTTGCAAACTATTTTTCGGGTTATGCATGCAGCGACTTCTCTTCTCTTGTGGCAA
AAATGGAAAATCCCGATGGAGGTGAGTCTTTGCTCGTGCTAAAAAGGACTCCATTTTTGTCTTTTTGCGAACACCATGTT
TTACCCATTTCAGGGTATATTTCTATCGGATACATTCCGGATAAGAGTATCACAAGTCTTGGGAGTCTAGGAAGATTGGT
AAGTGCTTGTACTAAAAGACTGCAGTTACAAGAGAGGATTTGTGCTCAGATTGCACTTGCAATTGAGGAATCTCTGTCTC
CAAAAGGAGTAATAGTGTATGCTTCAGCCAAGCATGCTTGCGTGGCGCATAATACCTCGGTCTTTGAGTCTGTAGCAAAA
AGAGGGGCTTTCGCTGCTAACACCAAAGGAGAAGTTCAAGAATTTTTTTCTATCTTAAAATAA

Upstream 100 bases:

>100_bases
GCAACTAAAAATGCTCAGGAAGTGGGTGATAAGCTGAGACGTCGCTATAACAGCGCAAGGCAGGAGAAGATTACAAAGGA
ATTGATAGAGGTTGTCAGTG

Downstream 100 bases:

>100_bases
ATTCTCATTCCTATTAATTAGAAATTTTTGAGGATTATACTCTCTGAAATTGTGTTGCGTGGTGCTTCCGGATAAGTGGT
GCATCTTTTTATGGGGCTGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MQKLSEGDIQDFFGVGVGETDRLGARGASHSFLKMFANYFSGYACSDFSSLVAKMENPDGGESLLVLKRTPFLSFCEHHV
LPISGYISIGYIPDKSITSLGSLGRLVSACTKRLQLQERICAQIALAIEESLSPKGVIVYASAKHACVAHNTSVFESVAK
RGAFAANTKGEVQEFFSILK

Sequences:

>Translated_180_residues
MQKLSEGDIQDFFGVGVGETDRLGARGASHSFLKMFANYFSGYACSDFSSLVAKMENPDGGESLLVLKRTPFLSFCEHHV
LPISGYISIGYIPDKSITSLGSLGRLVSACTKRLQLQERICAQIALAIEESLSPKGVIVYASAKHACVAHNTSVFESVAK
RGAFAANTKGEVQEFFSILK
>Mature_180_residues
MQKLSEGDIQDFFGVGVGETDRLGARGASHSFLKMFANYFSGYACSDFSSLVAKMENPDGGESLLVLKRTPFLSFCEHHV
LPISGYISIGYIPDKSITSLGSLGRLVSACTKRLQLQERICAQIALAIEESLSPKGVIVYASAKHACVAHNTSVFESVAK
RGAFAANTKGEVQEFFSILK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=87, Percent_Identity=42.5287356321839, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI4503949, Length=87, Percent_Identity=42.5287356321839, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI66932972, Length=85, Percent_Identity=42.3529411764706, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI66932970, Length=82, Percent_Identity=42.6829268292683, Blast_Score=80, Evalue=9e-16,
Organism=Escherichia coli, GI1788476, Length=120, Percent_Identity=31.6666666666667, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560486, Length=87, Percent_Identity=40.2298850574713, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321706, Length=131, Percent_Identity=30.5343511450382, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24656782, Length=134, Percent_Identity=35.8208955223881, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656796, Length=134, Percent_Identity=35.8208955223881, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656791, Length=134, Percent_Identity=35.8208955223881, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 19434; Mature: 19434

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLSEGDIQDFFGVGVGETDRLGARGASHSFLKMFANYFSGYACSDFSSLVAKMENPDG
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
GESLLVLKRTPFLSFCEHHVLPISGYISIGYIPDKSITSLGSLGRLVSACTKRLQLQERI
CCEEEEEECCCHHHHHHHCCCCEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CAQIALAIEESLSPKGVIVYASAKHACVAHNTSVFESVAKRGAFAANTKGEVQEFFSILK
HHHHHHHHHHCCCCCCEEEEECCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MQKLSEGDIQDFFGVGVGETDRLGARGASHSFLKMFANYFSGYACSDFSSLVAKMENPDG
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
GESLLVLKRTPFLSFCEHHVLPISGYISIGYIPDKSITSLGSLGRLVSACTKRLQLQERI
CCEEEEEECCCHHHHHHHCCCCEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CAQIALAIEESLSPKGVIVYASAKHACVAHNTSVFESVAKRGAFAANTKGEVQEFFSILK
HHHHHHHHHHCCCCCCEEEEECCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]