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The map label for this gene is tsf

Identifier: 88608372

GI number: 88608372

Start: 834437

End: 835309

Strand: Reverse

Name: tsf

Synonym: NSE_0947

Alternate gene names: 88608372

Gene position: 835309-834437 (Counterclockwise)

Preceding gene: 88608048

Following gene: 88608491

Centisome position: 97.24

GC content: 44.22

Gene sequence:

>873_bases
ATGAGTAAGATAAACTTGGAAGATCTAAAAAGTTTGTCAAAGGAGACTTCTGCTGGTTTGGTCCATTGCAAGCAGGCTCT
GAGCGAGGCGCATGGTGATCTGACTAGAGCAAGGGAAATACTAAAGGAGTTAGGGCATGCGGTTTCTGTGAAGAAGGCCA
ATAGGGGTGCTCGAGATGGTGTCGTTGGGGCTCTCTCTTCTGGAAAATTTGGTGTTATACTTGAGCTGAATTGCGAGACG
GACTTTGTTGCACGTAATGAAAAATTTCAGCAGTTCGCACAGTCGGTGCTTGAAGCTGCGTGTGCTGCCAAAGTCAAGTC
AGTTGAAGAGTGTCTTAGTACTCCGTTGCCAGGAGGTCAAAAGGTCCGCGACGCAATAGTGGAGCAAGTCGCAGTTTTTA
GGGAGAATATAGTTCTTTCGAGGTGTGTTACTTACGAGGTTTCTCAGAGTGGTTTGTTGGGTGTCTATGTTCACAACAAG
TACACCGAAAATCTTGGTAAGATAGGTGTTGCAGTTGCGGTGGTGTCAGAAGCTGATCCTTCCTTTCTTTCCACTGTTGC
AAAAGATATTGCTATTCAGGTGATGTCGGAGTGTCCATGCGCAATCGATGTCGCTCGTATACCACCAAACCTTCTTGAAT
CTGAAAAGCACAAATACAATCTGGAGGTCGAGGGAAAGCCTGCTTCGGTTGCTGAAAAAATTATTGCTGGCAAACTGAGT
AAATTCTATAAAAAAGTTGTCCTCCTAGAACAGCCTCTATTTTCAGATCCTGAGAGGTCCGTAAAGCAATATATTTCTGA
CAAGGAAATGGAATCTTCAGCTAAGATTGATGTAGTTTGGTATGAGGTGTTTGTTCTAGGAGAAGCTAGTTGA

Upstream 100 bases:

>100_bases
CAGAAACGAAAAGTTTCTCCGAAATCGGAAAAACAAGATAATGTGGATGCAGCTAAGCTTCCTGAGAACAAAGGTTGATG
TATAAGGCAGTGTAATTTCA

Downstream 100 bases:

>100_bases
GTCTCTATGCGGAGAGTTCTGCTGAAAATCTCAGGGGAAAGATTGGCTGCACGTGGGGTCTCTGTTCACGATGCTGAGGT
CATCGGTATGCTTGCTAGGG

Product: translation elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSKINLEDLKSLSKETSAGLVHCKQALSEAHGDLTRAREILKELGHAVSVKKANRGARDGVVGALSSGKFGVILELNCET
DFVARNEKFQQFAQSVLEAACAAKVKSVEECLSTPLPGGQKVRDAIVEQVAVFRENIVLSRCVTYEVSQSGLLGVYVHNK
YTENLGKIGVAVAVVSEADPSFLSTVAKDIAIQVMSECPCAIDVARIPPNLLESEKHKYNLEVEGKPASVAEKIIAGKLS
KFYKKVVLLEQPLFSDPERSVKQYISDKEMESSAKIDVVWYEVFVLGEAS

Sequences:

>Translated_290_residues
MSKINLEDLKSLSKETSAGLVHCKQALSEAHGDLTRAREILKELGHAVSVKKANRGARDGVVGALSSGKFGVILELNCET
DFVARNEKFQQFAQSVLEAACAAKVKSVEECLSTPLPGGQKVRDAIVEQVAVFRENIVLSRCVTYEVSQSGLLGVYVHNK
YTENLGKIGVAVAVVSEADPSFLSTVAKDIAIQVMSECPCAIDVARIPPNLLESEKHKYNLEVEGKPASVAEKIIAGKLS
KFYKKVVLLEQPLFSDPERSVKQYISDKEMESSAKIDVVWYEVFVLGEAS
>Mature_289_residues
SKINLEDLKSLSKETSAGLVHCKQALSEAHGDLTRAREILKELGHAVSVKKANRGARDGVVGALSSGKFGVILELNCETD
FVARNEKFQQFAQSVLEAACAAKVKSVEECLSTPLPGGQKVRDAIVEQVAVFRENIVLSRCVTYEVSQSGLLGVYVHNKY
TENLGKIGVAVAVVSEADPSFLSTVAKDIAIQVMSECPCAIDVARIPPNLLESEKHKYNLEVEGKPASVAEKIIAGKLSK
FYKKVVLLEQPLFSDPERSVKQYISDKEMESSAKIDVVWYEVFVLGEAS

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=285, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI291084500, Length=306, Percent_Identity=25.1633986928105, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI291084498, Length=95, Percent_Identity=41.0526315789474, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI291084502, Length=95, Percent_Identity=41.0526315789474, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786366, Length=279, Percent_Identity=34.4086021505376, Blast_Score=136, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19921466, Length=92, Percent_Identity=40.2173913043478, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_NEOSM (Q2GCI5)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506811.1
- STRING:   Q2GCI5
- GeneID:   3932029
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0947
- TIGR:   NSE_0947
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   NSEN222891:NSE_0947-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 31559; Mature: 31427

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKINLEDLKSLSKETSAGLVHCKQALSEAHGDLTRAREILKELGHAVSVKKANRGARDG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VVGALSSGKFGVILELNCETDFVARNEKFQQFAQSVLEAACAAKVKSVEECLSTPLPGGQ
CEEEECCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KVRDAIVEQVAVFRENIVLSRCVTYEVSQSGLLGVYVHNKYTENLGKIGVAVAVVSEADP
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEECHHHHHHHHHHHHEEEECCCCH
SFLSTVAKDIAIQVMSECPCAIDVARIPPNLLESEKHKYNLEVEGKPASVAEKIIAGKLS
HHHHHHHHHHHHHHHHHCCCEEEHHHCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
KFYKKVVLLEQPLFSDPERSVKQYISDKEMESSAKIDVVWYEVFVLGEAS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCC
>Mature Secondary Structure 
SKINLEDLKSLSKETSAGLVHCKQALSEAHGDLTRAREILKELGHAVSVKKANRGARDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VVGALSSGKFGVILELNCETDFVARNEKFQQFAQSVLEAACAAKVKSVEECLSTPLPGGQ
CEEEECCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KVRDAIVEQVAVFRENIVLSRCVTYEVSQSGLLGVYVHNKYTENLGKIGVAVAVVSEADP
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEECHHHHHHHHHHHHEEEECCCCH
SFLSTVAKDIAIQVMSECPCAIDVARIPPNLLESEKHKYNLEVEGKPASVAEKIIAGKLS
HHHHHHHHHHHHHHHHHCCCEEEHHHCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
KFYKKVVLLEQPLFSDPERSVKQYISDKEMESSAKIDVVWYEVFVLGEAS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA